FastQCFastQC Report
Mon 6 Feb 2017
SRR2077422_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077422_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3647695
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49450.025.5822411
GGTATCA21250.024.7443491
CGCACGA3250.023.01772535
TATCAAC67000.018.617762
TCAACGC68150.018.0135544
ATCAACG68950.017.8673843
CAACGCA69000.017.6636265
CGAATCG3700.017.2440537
AACGCAG71200.017.054876
TATACCG953.348835E-416.2103965
GTACATG91900.015.3933271
TACATGG93000.014.6902052
CGTGCCG3900.014.66795710
GTACCGA4650.014.6655436
ACGCAGA82350.014.5058587
TACCGAT4550.014.5049787
GCGCACG5350.014.394381534
CAACGAC4800.014.20802218
ATCGTTG5900.014.17015622
ACATGGG96350.014.0192213
Produced by FastQC (version 0.11.5)