FastQCFastQC Report
Mon 6 Feb 2017
SRR2077422_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077422_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3647695
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38010.10420279107765315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23450.025.7087061
GTATCAA52950.025.4306771
GCACCGT5250.018.856726
CGTGCCG3550.017.9726610
TATCAAC77000.017.3724692
GTACCGA4600.017.2170056
ATCAACG78600.017.0472893
TCAACGC78750.016.986884
CAACGCA79050.016.783945
AACGCAG80600.016.4313956
GCGTTAG953.3457056E-416.2123851
CGCACGA4350.016.18088235
CGAATCG4150.015.90020637
ACCGTAT1401.5879596E-615.7145818
ACCGTGC7850.015.1340428
GCGCACG5100.015.09521734
AGCACCG6500.014.8942215
GTACATG87400.014.6515671
AACGACT4800.014.21191219
TAATCGG1554.5592133E-614.19751415
Produced by FastQC (version 0.11.5)