FastQCFastQC Report
Mon 6 Feb 2017
SRR2077421_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077421_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983055
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58900.018.676471
TATACCG1602.1334927E-816.4995545
GGTATCA33850.016.183811
ATACCGT2051.05501385E-1016.0952366
GTACATG86800.015.15728951
TATCAAC74050.014.8549252
TACATGG87000.014.8184872
TTATACC3750.014.6665154
TCAACGC74500.014.587774
ATCAACG75050.014.5392473
CAACGCA75100.014.4416875
GGACCGT3250.014.213336
ACCGTAT2803.6379788E-1214.141058
AACGCAG76300.014.12646
TCGCTAA1251.6722266E-414.08009059
ACATGGG90250.013.9187363
GACCGTA1901.7586171E-713.8927297
GTATAGT4550.013.5390031
CCGTATA1302.3321787E-413.5385499
AGAGTAC73350.013.40726511
Produced by FastQC (version 0.11.5)