FastQCFastQC Report
Mon 6 Feb 2017
SRR2077421_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077421_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983055
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38560.12926345642302942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67000.020.8568361
GGTATCA35400.018.6495111
CGCGTAA600.007412144418.33438110
TATACCG1259.429346E-615.8435625
ATACCGT1552.6693306E-715.6137956
TATCAAC88800.015.5367572
ATCAACG91300.015.35259153
TCAACGC91950.015.1962014
GTACATG84450.015.1660621
CAACGCA92150.015.0679575
ACCGTAT2051.8117134E-915.0249988
TACATGG85100.014.8677082
AACGCAG94500.014.7373516
CGTCGAA950.00624099613.89529922
ACATGGG89150.013.8716483
CATGGGG59600.013.5128794
TATACTG6550.013.4381355
CTTAGAC5750.013.3940923
GCGTCGA1150.001424288613.39184621
ACGATCG1000.00878542313.19522343
Produced by FastQC (version 0.11.5)