FastQCFastQC Report
Mon 6 Feb 2017
SRR2077420_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077420_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257056
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28900.12804290190407328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16700.030.1707211
GTATCAA40250.025.0360761
TATCAAC52200.018.7550262
TCAACGC52900.018.3833074
ATCAACG53300.018.3266853
CAACGCA54600.017.8504375
GCGATAC1002.4838171E-517.60061314
ACCGTGC2700.017.1109498
AACGCAG56900.017.0504256
GTACATG68250.016.086611
TACATGG69000.015.6871542
GCACCGT3000.015.3995116
ACGCAGA64250.014.8263187
ACATGGG70900.014.7702853
CGCAGAG65700.014.2647048
GTGGTAT6500.014.2168251
CCTATAC3150.013.96843053
GAGTACT35350.013.81660512
CATGGGT13850.013.3440174
AGAGTAC67650.013.17118911
Produced by FastQC (version 0.11.5)