##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077420_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2257056 Sequences flagged as poor quality 0 Sequence length 50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.063335158720033 31.0 30.0 34.0 23.0 34.0 2 29.084075893553372 31.0 30.0 34.0 22.0 34.0 3 29.159717348616958 31.0 30.0 34.0 22.0 34.0 4 32.515327488551456 35.0 33.0 37.0 23.0 37.0 5 32.60254464222421 35.0 33.0 37.0 25.0 37.0 6 32.679281772361875 37.0 33.0 37.0 25.0 37.0 7 32.53241523471283 37.0 33.0 37.0 23.0 37.0 8 32.453547896020304 37.0 33.0 37.0 22.0 37.0 9 33.65639576510286 38.0 34.0 39.0 19.0 39.0 10 33.56568202118157 38.0 34.0 39.0 18.0 39.0 11 33.63685703854933 38.0 34.0 39.0 19.0 39.0 12 33.55231283583571 38.0 34.0 39.0 18.0 39.0 13 33.56383891228219 38.0 34.0 39.0 18.0 39.0 14 34.409823681822694 39.0 34.0 41.0 17.0 41.0 15 34.513258421589896 39.0 34.0 41.0 17.0 41.0 16 34.40748435129655 39.0 34.0 41.0 17.0 41.0 17 34.235311839848016 39.0 33.0 41.0 17.0 41.0 18 34.06216637956701 39.0 33.0 41.0 17.0 41.0 19 34.42917942665135 39.0 34.0 41.0 18.0 41.0 20 34.46809693689479 39.0 34.0 41.0 18.0 41.0 21 34.47971871322643 39.0 34.0 41.0 18.0 41.0 22 34.39283163554648 39.0 34.0 41.0 18.0 41.0 23 34.13673519841776 39.0 33.0 41.0 18.0 41.0 24 34.29001274226249 39.0 34.0 41.0 18.0 41.0 25 34.194854270341544 39.0 33.0 41.0 18.0 41.0 26 34.24497575602909 39.0 34.0 41.0 18.0 41.0 27 34.22525360469567 39.0 34.0 41.0 18.0 41.0 28 34.10854537946776 39.0 33.0 41.0 17.0 41.0 29 34.07195169282463 39.0 33.0 41.0 16.0 41.0 30 33.844527118511905 39.0 33.0 41.0 16.0 41.0 31 33.74948694228234 39.0 33.0 41.0 15.0 41.0 32 33.766188344462876 39.0 33.0 41.0 15.0 41.0 33 33.74592566600031 38.0 33.0 41.0 15.0 41.0 34 33.73433756185048 38.0 33.0 41.0 15.0 41.0 35 33.66762277940822 38.0 33.0 41.0 10.0 41.0 36 33.53365002906441 38.0 33.0 41.0 9.0 41.0 37 33.44170725050685 38.0 33.0 41.0 9.0 41.0 38 33.32789084541987 38.0 33.0 40.0 8.0 41.0 39 33.267267183446044 38.0 33.0 40.0 8.0 41.0 40 33.2165652956772 38.0 33.0 40.0 7.0 41.0 41 33.09082140629209 38.0 32.0 40.0 2.0 41.0 42 32.90502805424411 38.0 32.0 40.0 2.0 41.0 43 32.80072581274013 38.0 32.0 40.0 2.0 41.0 44 32.726706825174034 38.0 32.0 40.0 2.0 41.0 45 32.628936986942286 38.0 32.0 40.0 2.0 41.0 46 32.45772103129032 38.0 32.0 40.0 2.0 41.0 47 32.25717704833199 38.0 31.0 40.0 2.0 41.0 48 32.060486314916425 38.0 31.0 40.0 2.0 41.0 49 31.924318669984263 38.0 31.0 40.0 2.0 41.0 50 31.71339966753151 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0935347437792053 1101 2 1.035534299694156 1101 3 1.0870828029792143 1101 4 1.095701528555054 1101 5 0.9628348629582106 1101 6 1.0054391634008724 1101 7 1.0398918787894118 1101 8 1.03041535268504 1101 9 1.3821812040266295 1101 10 1.4020547606279976 1101 11 1.3039107878415876 1101 12 1.2215684061015182 1101 13 1.2878294380142847 1101 14 1.645639730377539 1101 15 1.5578129397311145 1101 16 1.5931105507334067 1101 17 1.6347168792005178 1101 18 1.8295968148913033 1101 19 1.3818889458504415 1101 20 1.4394174521685201 1101 21 1.3871269000568844 1101 22 1.3477135270362908 1101 23 1.5118765299999453 1101 24 1.4275484557377638 1101 25 1.4360285177319128 1101 26 1.500662049623493 1101 27 1.461199693935022 1101 28 1.6199621992989535 1101 29 1.640142541048391 1101 30 1.712453178219107 1101 31 1.786585787943821 1101 32 1.7514394547360084 1101 33 1.7107694192546035 1101 34 1.649425161902073 1101 35 1.6221575704509732 1101 36 1.6081491886354513 1101 37 1.6616012021154845 1101 38 1.7176351541974384 1101 39 1.6910759789888559 1101 40 1.676105978296782 1101 41 1.6998700717705333 1101 42 1.6995590808222119 1101 43 1.6235201264867172 1101 44 1.5052714895762307 1101 45 1.657708018813949 1101 46 1.6865262567027273 1101 47 1.6465067788211414 1101 48 1.6837277272849391 1101 49 1.710122153453483 1101 50 1.7344356435542956 1102 1 0.8359461953202398 1102 2 0.8576025662409918 1102 3 0.7629680446220739 1102 4 0.8920925787630267 1102 5 0.8525775563118358 1102 6 0.952714762015539 1102 7 0.9478680656453378 1102 8 1.0674491597395246 1102 9 1.1300106208478837 1102 10 1.151261937209874 1102 11 0.8766753273916166 1102 12 0.8819443420017592 1102 13 1.0432223755839019 1102 14 1.325195730832938 1102 15 1.2554415861428865 1102 16 1.3330514844986467 1102 17 1.4089851087356564 1102 18 1.5004995524746505 1102 19 1.3272359656344364 1102 20 1.231310635323858 1102 21 1.2169370438235987 1102 22 1.3348300574810068 1102 23 1.480388696064317 1102 24 1.3149511523971356 1102 25 1.2572338204398719 1102 26 1.0801928254976048 1102 27 1.1388043288240723 1102 28 1.1845519136513047 1102 29 1.0761064103332814 1102 30 1.3363038198793404 1102 31 1.2985553167166373 1102 32 1.207691561147719 1102 33 1.0969942846264615 1102 34 1.0267539013624685 1102 35 1.192921903747532 1102 36 1.169335553220371 1102 37 1.2420730199080552 1102 38 1.334405236620917 1102 39 1.3840712107806397 1102 40 1.1836793425798646 1102 41 1.2916993180263674 1102 42 1.254962054608498 1102 43 1.3893300370244859 1102 44 1.3256510244403117 1102 45 1.278959799760699 1102 46 1.1483256766295113 1102 47 1.2180708260101198 1102 48 1.2241973468277152 1102 49 1.2147790668946357 1102 50 1.13872921746864 1103 1 0.6156111373798439 1103 2 0.6107909385480426 1103 3 0.7206906629837384 1103 4 0.7222988113569002 1103 5 0.6069505956216688 1103 6 0.625609621005907 1103 7 0.8009501005216109 1103 8 0.8338844449599279 1103 9 0.9453518709679258 1103 10 0.9127017140113054 1103 11 0.9567167519730688 1103 12 0.9868480180957491 1103 13 0.9020191720562636 1103 14 1.1846440066260229 1103 15 0.9787201802080006 1103 16 1.1423518582301995 1103 17 1.2415984386277827 1103 18 1.1319600765791336 1103 19 0.958956831379183 1103 20 1.0063774588457406 1103 21 0.9164292650854833 1103 22 1.014785151674161 1103 23 1.1754235983499228 1103 24 1.0947573941942466 1103 25 0.8372407328730489 1103 26 0.8697427470699992 1103 27 0.8443866938165527 1103 28 0.9740180557333176 1103 29 0.973158672729646 1103 30 1.1052895028397458 1103 31 1.0108315379465083 1103 32 0.8843429924515434 1103 33 0.9119681554289869 1103 34 0.9030333517290003 1103 35 0.6922629217858827 1103 36 0.954448412946995 1103 37 0.9443874845996305 1103 38 0.891931235772617 1103 39 0.9713816940707574 1103 40 0.9446279464884455 1103 41 1.060936589545939 1103 42 1.0201036344446024 1103 43 1.1025796008709605 1103 44 1.075594254457279 1103 45 0.895385281530281 1103 46 0.8991492165155464 1103 47 0.8759834112401208 1103 48 0.9842964016950262 1103 49 0.8827864554954203 1103 50 0.8581982205577283 1104 1 0.6216563653000726 1104 2 0.5962209599155663 1104 3 0.6398591102771825 1104 4 0.5792432558013445 1104 5 0.5883341426302167 1104 6 0.6399614728577561 1104 7 0.8199894523734628 1104 8 0.7306169948174741 1104 9 1.0149877327912833 1104 10 1.0611214077434994 1104 11 0.9479516166454331 1104 12 0.8503140081242364 1104 13 0.8137624056744954 1104 14 1.1650081448026555 1104 15 1.1151874166752336 1104 16 1.071954778458121 1104 17 1.282566209283054 1104 18 1.3159834027899606 1104 19 1.0251872444845915 1104 20 0.9057720457403278 1104 21 0.9542141653703808 1104 22 1.148959831161342 1104 23 1.184803049916873 1104 24 1.140185634934987 1104 25 1.3330429052377184 1104 26 1.0547854821127345 1104 27 1.1136530755829313 1104 28 1.2758832622860226 1104 29 1.2670600260059999 1104 30 1.4494668532670971 1104 31 1.5134757687157432 1104 32 1.4134695950156413 1104 33 1.3492366739475017 1104 34 1.3120255169711683 1104 35 1.3532983562588186 1104 36 1.3303298232034066 1104 37 1.3231223492719906 1104 38 1.320301071954951 1104 39 1.2210122140137827 1104 40 1.2642549051779 1104 41 1.4423379428222916 1104 42 1.3566554506839168 1104 43 1.5230122931786525 1104 44 1.4746282644287874 1104 45 1.3263236718436744 1104 46 1.4044510327548636 1104 47 1.4110795650862755 1104 48 1.4723038803275088 1104 49 1.350321177717646 1104 50 1.4101079427799483 1105 1 0.6500185684212099 1105 2 0.7060418589593809 1105 3 0.6427389786703834 1105 4 0.6477061032205143 1105 5 0.6375798759939286 1105 6 0.5352661933468212 1105 7 0.7718644129835326 1105 8 0.7635350169016917 1105 9 0.8945206449957794 1105 10 0.978082266678264 1105 11 0.9319925142994876 1105 12 0.9911315087261983 1105 13 0.9340316954276204 1105 14 1.191258724541612 1105 15 1.1854700384042545 1105 16 1.0692622041262965 1105 17 1.0610338562135482 1105 18 1.2521914333258408 1105 19 1.0725091333266192 1105 20 1.041299256707127 1105 21 1.0560468676568533 1105 22 1.1518868866692245 1105 23 1.5007661922555116 1105 24 1.1839137078746305 1105 25 1.1653882179573927 1105 26 1.0963083654393557 1105 27 0.9986626018857123 1105 28 1.1283650297989851 1105 29 0.9393755720594967 1105 30 1.35201948225194 1105 31 1.2942168003359527 1105 32 1.1540348384736845 1105 33 1.1273358735441477 1105 34 1.1524260903269266 1105 35 1.1561523686987627 1105 36 1.1639611463979733 1105 37 1.0471224914797332 1105 38 1.1900576866349937 1105 39 1.1887417219588272 1105 40 1.1722195083779 1105 41 1.2590538165999945 1105 42 1.2170888934202537 1105 43 1.1270029979634586 1105 44 1.0420584897149396 1105 45 1.1601253211302947 1105 46 1.1677709694724214 1105 47 1.2743002795832226 1105 48 1.4183809666201341 1105 49 1.2249918779130198 1105 50 1.111849502831209 1106 1 0.44781582785380536 1106 2 0.5180591547950399 1106 3 0.5716352127356785 1106 4 0.6081396332234164 1106 5 0.4348074515298137 1106 6 0.537340486243508 1106 7 0.47622833658201813 1106 8 0.57326155110988 1106 9 -0.7348914186042919 1106 10 -0.3444457886408827 1106 11 0.32943045793893333 1106 12 0.5336481815173855 1106 13 0.6657973905654231 1106 14 0.8397929094779002 1106 15 0.7612250945579007 1106 16 0.9054602040444237 1106 17 1.0520432360473606 1106 18 -0.7440849540770458 1106 19 0.29203128378019017 1106 20 0.6071079808589062 1106 21 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0.2970733032720929 1107 9 0.4978534292048238 1107 10 0.6597089681430859 1107 11 0.6498643807089124 1107 12 0.6283665542546828 1107 13 0.5689891559054772 1107 14 0.959681884522972 1107 15 0.7807241316615716 1107 16 0.6394240388994419 1107 17 0.9707531000101639 1107 18 0.8987574293404492 1107 19 0.7805548768208936 1107 20 0.8183756869637833 1107 21 0.7492157454693356 1107 22 0.8331537756431757 1107 23 0.956145120835572 1107 24 0.8330855628052802 1107 25 0.8199719889491917 1107 26 0.7275296194631338 1107 27 0.7136378776845831 1107 28 0.7741381575773261 1107 29 0.7612262041687927 1107 30 1.0112827367595258 1107 31 0.955058907250617 1107 32 1.0703403718694844 1107 33 0.9374575790185347 1107 34 0.7338313650259565 1107 35 0.6845763547679766 1107 36 0.7751271966205948 1107 37 0.72557451810858 1107 38 0.8331104878973079 1107 39 0.832059879930199 1107 40 0.8565486057696532 1107 41 0.8199738735488253 1107 42 0.9932683939861064 1107 43 0.9847705211519511 1107 44 0.9726004305635243 1107 45 0.9317514926821673 1107 46 0.9799763855029084 1107 47 1.1967949880582651 1107 48 1.0902060396352553 1107 49 1.2100257595221393 1107 50 1.2471324355199407 1108 1 0.07009369794792875 1108 2 0.1900581169106168 1108 3 0.13358487768716287 1108 4 0.08130396312520816 1108 5 0.19540523917499542 1108 6 0.2511839735539425 1108 7 0.21659207002871028 1108 8 0.1982197624202442 1108 9 0.3640527029088858 1108 10 0.24307843706895227 1108 11 0.32502249897947166 1108 12 0.24775418977494468 1108 13 0.5027005630337058 1108 14 0.25501307164969234 1108 15 0.33168592791059126 1108 16 0.3512812998899051 1108 17 0.1597249180207072 1108 18 0.662955533972621 1108 19 0.3788041928383876 1108 20 0.3839011291325676 1108 21 0.6007964330052786 1108 22 0.4778547179509687 1108 23 0.13387658846409778 1108 24 0.3263454789895732 1108 25 0.3839308087571993 1108 26 0.3705085504673775 1108 27 0.33653720899338424 1108 28 0.40867892666063454 1108 29 0.3474233274219358 1108 30 0.42543883450223774 1108 31 0.5321913611155438 1108 32 0.47361782884808434 1108 33 0.5608211907951741 1108 34 0.4248674508657331 1108 35 0.3734341658683107 1108 36 0.3565256546623914 1108 37 0.3466148411855414 1108 38 0.2487803899079566 1108 39 0.5113448832938161 1108 40 0.4644626895962105 1108 41 0.43180734281801136 1108 42 0.5101460147008012 1108 43 0.5511445897320044 1108 44 0.5597277653177599 1108 45 0.4073553107700505 1108 46 0.4112693606258233 1108 47 0.39017688102570247 1108 48 0.4719782800019061 1108 49 0.311668991697033 1108 50 0.5333605186641073 1109 1 0.04269106371071629 1109 2 0.01747873836426095 1109 3 -0.06711100274843673 1109 4 0.013025145344379041 1109 5 -0.07608087729363433 1109 6 -0.04390919343511257 1109 7 -0.043275663036311585 1109 8 0.0220546510154378 1109 9 0.10039360188092417 1109 10 -0.08407015115705718 1109 11 -0.0798114249107158 1109 12 0.02943481878415355 1109 13 -0.03068036147642772 1109 14 -0.7042465857640963 1109 15 -0.0945907253148448 1109 16 -1.1561633700973672 1109 17 -0.9526282010164877 1109 18 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-2.202355385673904 2216 21 -2.470520819516885 2216 22 -2.5289598544360103 2216 23 -4.319019964819848 2216 24 -3.0490651640873843 2216 25 -2.5910161645700818 2216 26 -2.5772867697564763 2216 27 -2.563032463919864 2216 28 -3.0982949377805014 2216 29 -2.646591867785485 2216 30 -2.5515127626236875 2216 31 -2.4354782712281455 2216 32 -2.6344648779188304 2216 33 -2.7916826717941774 2216 34 -3.071309804879384 2216 35 -3.1187638807145888 2216 36 -3.0165429623115116 2216 37 -2.7763615401230624 2216 38 -2.982973292804182 2216 39 -3.17486143198677 2216 40 -2.9393654615769833 2216 41 -3.0888125409297125 2216 42 -3.288014961626086 2216 43 -3.437891469819018 2216 44 -3.353253097018566 2216 45 -3.2739398970747153 2216 46 -3.563245324281592 2216 47 -3.3024042086132326 2216 48 -3.436338938594499 2216 49 -3.769440525610051 2216 50 -3.6314130198070025 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 109885.0 3 5412.0 4 10668.0 5 8529.0 6 6811.0 7 5878.0 8 5247.0 9 4779.0 10 4608.0 11 4521.0 12 4628.0 13 4969.0 14 5265.0 15 5690.0 16 6127.0 17 6608.0 18 7296.0 19 8469.0 20 10970.0 21 14773.0 22 19015.0 23 22102.0 24 23145.0 25 23635.0 26 25267.0 27 28629.0 28 33160.0 29 37617.0 30 42637.0 31 50641.0 32 61484.0 33 76026.0 34 95887.0 35 122038.0 36 158055.0 37 215085.0 38 337277.0 39 644223.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.76107256334447 18.092047367424723 12.938173283626886 24.20870678560393 2 15.758793506442837 20.17239422432353 39.35544138667309 24.713370882560536 3 18.646203336389412 25.436910248748458 29.356290000230533 26.560596414631593 4 12.398805306242258 16.73382680665063 37.90780497191955 32.95956291518756 5 13.77918172986151 38.0352524209379 34.2130992927079 13.97246655649269 6 31.653644471005073 37.04471820807967 17.89730257896221 13.404334741953045 7 27.962069132122842 32.57106940206977 21.703245435835743 17.763616029971644 8 26.385365107582775 34.480572592604084 20.168018424395033 18.966043875418105 9 27.06448169407192 15.748089789216285 19.075686727204697 38.1117417895071 10 14.561845215057408 28.971757934782076 33.81151947125541 22.654877378905102 11 34.62134181859126 23.51024055848081 23.558515387213774 18.309902235714155 12 23.43079557314412 25.312324595899987 30.050019818169403 21.20686001278649 13 27.732692199414167 22.475701447117757 25.880713091536705 23.910893261931374 14 22.23374919089547 21.560751558357495 27.209897232640827 28.995602018106208 15 23.673802387244383 29.166548427717576 23.832937232853872 23.32671195218417 16 23.40982399978718 28.469026489816642 25.290603198109423 22.830546312286764 17 22.42358588830421 27.96314616267711 26.534922505042836 23.078345443975845 18 22.28636691523043 27.546507611868105 27.63562346291783 22.531502009983637 19 23.26672051968458 27.140945643624846 27.81551232929672 21.776821507393855 20 23.583701330287372 26.64396976446273 27.555940045059767 22.21638886019013 21 23.887199042256018 26.406863831862726 27.421096971578063 22.284840154303197 22 23.416257793566157 26.32352067450982 27.39965575737368 22.86056577455034 23 23.313845790965736 26.403905548230117 27.121320813777068 23.16092784702708 24 22.83551765061061 26.53179654573013 27.52820520095527 23.104480602703994 25 23.206578561148145 26.48991764383508 27.398965825783055 22.904537969233715 26 22.77034850076515 26.672895463960405 27.579988089309776 22.976767945964667 27 22.96655512314644 26.836510779165557 27.245631778685357 22.951302319002647 28 23.043406147001537 26.734010735788203 27.21922330510429 23.003359812105966 29 23.14031262375041 26.817936509414675 27.35557592471629 22.68617494211862 30 23.301341510565017 26.741578314492358 27.22300750919584 22.734072665746787 31 23.39373963041491 26.455022936125573 27.254661491517666 22.89657594194185 32 22.83283013864939 26.797212025059668 27.20315245668232 23.166805379608622 33 23.033671496727738 26.554014507919057 27.273158576166573 23.139155419186636 34 23.276640190951444 26.601488172820986 27.315827086802397 22.806044549425177 35 23.30718814558357 26.70272216499714 27.092527714505017 22.897561974914275 36 23.28603292994958 26.71321321987149 27.085792584774886 22.91496126540404 37 23.35213958430528 26.295742118137905 27.196881136570454 23.15523716098636 38 23.192317941519057 26.6201487762561 27.196072597705083 22.991460684519758 39 23.332576760384185 26.757458375927506 26.984873565134283 22.92509129855403 40 23.39242964302814 26.58646769874625 27.31996240534837 22.701140252877234 41 23.271493591786776 26.63445899129214 27.193423818045844 22.900623598875246 42 23.399742779585335 26.636987160702468 27.05615624389035 22.907113815821848 43 23.15263010229296 26.502160522691803 27.168777843129522 23.176431531885715 44 23.236046830136594 26.6093981173271 27.02147105206471 23.133084000471598 45 23.3667954147138 26.65807065865397 26.891262123872938 23.083871802759294 46 23.359974540745 26.52997091510597 26.769936803959304 23.34011774018972 47 23.277379266636085 26.694898540319063 26.807693074770146 23.22002911827471 48 23.287393739201047 26.87085052078762 26.80883827436629 23.03291746564504 49 23.329417657775867 26.70684297549969 26.653205608429488 23.31053375829495 50 23.535585949568123 26.76983413214023 26.573563887338754 23.121016030952898 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1448.0 1 1172.5 2 897.0 3 916.5 4 936.0 5 914.5 6 893.0 7 905.0 8 917.0 9 1029.5 10 1142.0 11 1508.0 12 1874.0 13 3258.0 14 4642.0 15 6736.0 16 8830.0 17 9833.0 18 10836.0 19 11676.5 20 12517.0 21 14124.5 22 15732.0 23 19527.5 24 23323.0 25 28350.0 26 33377.0 27 39048.0 28 44719.0 29 50449.0 30 56179.0 31 62160.5 32 68142.0 33 74717.5 34 81293.0 35 88847.0 36 96401.0 37 101345.0 38 106289.0 39 113033.5 40 119778.0 41 126009.5 42 132241.0 43 136668.5 44 141096.0 45 144838.0 46 148580.0 47 151812.0 48 155044.0 49 154786.5 50 154529.0 51 153038.0 52 151547.0 53 145553.0 54 139559.0 55 133448.5 56 127338.0 57 119089.5 58 110841.0 59 100416.0 60 89991.0 61 79191.0 62 68391.0 63 59084.5 64 49778.0 65 42163.0 66 34548.0 67 28943.5 68 23339.0 69 19306.5 70 15274.0 71 12324.0 72 9374.0 73 7935.5 74 6497.0 75 5048.5 76 3600.0 77 2856.5 78 2113.0 79 1694.5 80 1276.0 81 1033.5 82 791.0 83 663.5 84 536.0 85 393.0 86 250.0 87 194.5 88 139.0 89 117.0 90 95.0 91 70.0 92 45.0 93 35.0 94 25.0 95 18.0 96 11.0 97 15.5 98 20.0 99 21.5 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06260367487559015 2 0.06769880765031971 3 0.06397714544964768 4 0.0775346291806672 5 0.08130059688372819 6 0.0629138133923128 7 0.04683091602512299 8 0.05985673372747508 9 0.056888265067415254 10 0.06419867296159244 11 0.05414132391930018 12 0.06867352870287667 13 0.0650847830093715 14 0.06539492152609416 15 0.07691435214722188 16 0.07359143946805041 17 0.08316142798406419 18 0.06933811123871096 19 0.06796464066465342 20 0.08103476386939447 21 0.07780046219500092 22 0.07399018898955098 23 0.0708001928175464 24 0.060033955737030895 25 0.07664851913288814 26 0.08590836913217927 27 0.07678143564005502 28 0.09605432917924943 29 0.08918697630896176 30 0.07478768803255215 31 0.07363574497043937 32 0.07970559879772589 33 0.07691435214722188 34 0.06220492535408958 35 0.08103476386939447 36 0.08710461769668099 37 0.09445933109324714 38 0.07633838061616549 39 0.05626798803396991 40 0.06273659138275701 41 0.05666673755547049 42 0.06216061985170063 43 0.03938759162377894 44 0.039963563154835326 45 0.04297633731728411 46 0.039963563154835326 47 0.03283037727021394 48 0.04652077750840033 49 0.05166021578551883 50 0.04355230884834049 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2257056.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.70534190894141 #Duplication Level Percentage of deduplicated Percentage of total 1 77.42863764981966 48.55189197375476 2 12.605797037661686 15.809016265625939 3 4.450534166821997 8.372167998239972 4 2.0420576631449285 5.121916958611066 5 1.0853170690132459 3.4027588946042875 6 0.6277313980242756 2.3617267164053994 7 0.39330183253178613 1.7263488117623211 8 0.2811573736963736 1.4104055398280895 9 0.20133662009019382 1.136239344139162 >10 0.8337484496282219 8.880197561617827 >50 0.03698187395029629 1.592522446665528 >100 0.013043107214636166 1.3147329894761077 >500 1.423033610719347E-4 0.055546801169736634 >1k 2.134550416070587E-4 0.26452769809983456 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2890 0.12804290190407328 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 1.3291650716685806E-4 13 0.0 0.0 0.0 0.0 1.7722200955581075E-4 14 0.0 0.0 0.0 4.430550238895269E-5 1.7722200955581075E-4 15 0.0 8.861100477790538E-5 0.0 4.430550238895269E-5 2.658330143337161E-4 16 0.0 8.861100477790538E-5 0.0 8.861100477790538E-5 2.658330143337161E-4 17 0.0 8.861100477790538E-5 0.0 1.3291650716685806E-4 2.658330143337161E-4 18 0.0 8.861100477790538E-5 0.0 1.3291650716685806E-4 2.658330143337161E-4 19 0.0 8.861100477790538E-5 0.0 1.3291650716685806E-4 2.658330143337161E-4 20 0.0 8.861100477790538E-5 0.0 1.3291650716685806E-4 3.544440191116215E-4 21 0.0 8.861100477790538E-5 0.0 1.3291650716685806E-4 3.544440191116215E-4 22 0.0 8.861100477790538E-5 0.0 1.3291650716685806E-4 3.544440191116215E-4 23 0.0 8.861100477790538E-5 0.0 2.2152751194476345E-4 3.544440191116215E-4 24 0.0 8.861100477790538E-5 0.0 3.987495215005742E-4 3.987495215005742E-4 25 0.0 8.861100477790538E-5 0.0 4.8736052627847956E-4 4.430550238895269E-4 26 0.0 8.861100477790538E-5 0.0 7.08888038223243E-4 4.8736052627847956E-4 27 0.0 8.861100477790538E-5 0.0 7.08888038223243E-4 5.316660286674322E-4 28 0.0 8.861100477790538E-5 0.0 0.0010633320573348645 5.316660286674322E-4 29 0.0 8.861100477790538E-5 0.0 0.001993747607502871 5.316660286674322E-4 30 0.0 8.861100477790538E-5 0.0 0.0032343016743935464 5.316660286674322E-4 31 0.0 8.861100477790538E-5 0.0 0.006468603348787093 6.202770334453377E-4 32 0.0 8.861100477790538E-5 0.0 0.01054470956857074 6.202770334453377E-4 33 0.0 8.861100477790538E-5 0.0 0.015152481817021819 6.202770334453377E-4 34 0.0 8.861100477790538E-5 0.0 0.02126664114669729 6.202770334453377E-4 35 0.0 8.861100477790538E-5 0.0 0.0280010775098181 6.202770334453377E-4 36 0.0 8.861100477790538E-5 0.0 0.035488707413551104 6.202770334453377E-4 37 0.0 1.7722200955581075E-4 0.0 0.04528022344150965 6.202770334453377E-4 38 0.0 1.7722200955581075E-4 0.0 0.057951597124750114 6.645825358342903E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1670 0.0 30.170721 1 GTATCAA 4025 0.0 25.036076 1 TATCAAC 5220 0.0 18.755026 2 TCAACGC 5290 0.0 18.383307 4 ATCAACG 5330 0.0 18.326685 3 CAACGCA 5460 0.0 17.850437 5 GCGATAC 100 2.4838171E-5 17.600613 14 ACCGTGC 270 0.0 17.110949 8 AACGCAG 5690 0.0 17.050425 6 GTACATG 6825 0.0 16.08661 1 TACATGG 6900 0.0 15.687154 2 GCACCGT 300 0.0 15.399511 6 ACGCAGA 6425 0.0 14.826318 7 ACATGGG 7090 0.0 14.770285 3 CGCAGAG 6570 0.0 14.264704 8 GTGGTAT 650 0.0 14.216825 1 CCTATAC 315 0.0 13.9684305 3 GAGTACT 3535 0.0 13.816605 12 CATGGGT 1385 0.0 13.344017 4 AGAGTAC 6765 0.0 13.171189 11 >>END_MODULE