FastQCFastQC Report
Mon 6 Feb 2017
SRR2077420_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077420_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257056
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31480.13947372152042306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20700.029.0204871
GTATCAA48100.024.7951411
GTATACG550.00447943720.0041311
TATAGCG550.00447943720.0041315
TATCAAC68250.017.4086462
ATCAACG70600.016.7983883
TCAACGC70850.016.677374
CAACGCA72200.016.2443525
CATGGGT12250.016.1658864
AACGCAG74050.015.8654196
GTGGTAT7500.015.5498781
CCTATAC3000.015.4021573
GTACATG64450.014.6127921
ACAGCGT1851.2659439E-714.2713198
ATGGGTA6800.014.2382345
TACATGG65500.014.0749422
AATAGCG950.00623351313.8976075
TTACCGC1901.7518687E-713.89668316
TACCGCG1606.3388397E-613.75192517
ACATGGG65800.013.7435663
Produced by FastQC (version 0.11.5)