FastQCFastQC Report
Mon 6 Feb 2017
SRR2077419_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077419_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008231
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57890.28826365094453776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33580.1672118396738224No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25580.12737578495700944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24420.12159955702307154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29100.026.2312411
GTATCAA65400.024.1170251
TCGCGTC550.004483801320.00063517
TATCAAC87550.017.9638652
ATCAACG87500.017.5472093
TTAACCG1408.360439E-817.28927832
TCAACGC88650.017.2455714
TATTGCG902.2187065E-417.108675
CAACGCA90100.017.0652665
AACGCAG91400.016.6067736
GGTACGA1753.6288839E-916.34337618
TAACCGT1501.838689E-716.13384433
AACCGTG1105.6286786E-515.9985147
ACCGTGA1259.454727E-615.8385288
GTACATG112350.015.7813561
GGACCGT850.00298270415.5279696
TACATGG112500.015.4658512
AACCGTT1603.8256803E-715.12548134
ACCGTGC2203.0559022E-1014.9986088
TAGCCCT10000.014.9582384
Produced by FastQC (version 0.11.5)