FastQCFastQC Report
Mon 6 Feb 2017
SRR2077419_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077419_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008231
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72930.36315543381214616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43730.2177538340957788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42990.21406899903447363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34550.027.8307021
GCGTATC757.006565E-826.4041391
GTATCAA80500.024.81881
TTCGCGT500.002586520521.99194543
CGTATCA1152.0213156E-719.1320042
CGTTACG750.001290347917.60188324
CCGTATT1651.6589183E-917.3351927
TATCAAC116700.017.005682
TCGGCGT1053.7720798E-516.76411613
GTCGGCG1053.7720798E-516.76411612
ATCAACG120250.016.6321333
TAACCGT1753.6379788E-916.34012833
TCAACGC122150.016.3374024
CAACGCA122750.016.2220975
CGCACTG953.34433E-416.2126645
TATATCG953.3449646E-416.2122614
GTACATG106150.016.0439661
AACGCAG125800.015.9329446
CGAAATC1704.487447E-815.5314613
CGTATTA1857.565177E-915.46111428
Produced by FastQC (version 0.11.5)