##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077419_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2008231 Sequences flagged as poor quality 0 Sequence length 50 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.54806294694186 31.0 31.0 34.0 27.0 34.0 2 30.45556960329763 31.0 30.0 34.0 26.0 34.0 3 30.643468804136575 31.0 31.0 34.0 27.0 34.0 4 34.0800888941561 37.0 35.0 37.0 30.0 37.0 5 33.74662775348055 37.0 35.0 37.0 28.0 37.0 6 33.68176320353585 37.0 35.0 37.0 28.0 37.0 7 33.489024419999495 37.0 35.0 37.0 27.0 37.0 8 33.446723509397074 37.0 35.0 37.0 27.0 37.0 9 34.84558549290395 39.0 35.0 39.0 27.0 39.0 10 34.73385332663424 39.0 35.0 39.0 27.0 39.0 11 34.66695265634282 39.0 35.0 39.0 27.0 39.0 12 34.60581526726756 39.0 35.0 39.0 27.0 39.0 13 34.56545885408601 39.0 35.0 39.0 26.0 39.0 14 35.694157196059614 40.0 36.0 41.0 27.0 41.0 15 35.705525410174424 40.0 36.0 41.0 27.0 41.0 16 35.673347339026236 40.0 36.0 41.0 27.0 41.0 17 35.704986129583695 40.0 36.0 41.0 27.0 41.0 18 35.723487985196925 40.0 36.0 41.0 27.0 41.0 19 35.79823984392234 40.0 36.0 41.0 27.0 41.0 20 35.81846460890206 40.0 36.0 41.0 27.0 41.0 21 35.76950559970442 40.0 36.0 41.0 27.0 41.0 22 35.766249998132686 40.0 36.0 41.0 27.0 41.0 23 35.70588841622303 40.0 36.0 41.0 27.0 41.0 24 35.688576164793794 40.0 35.0 41.0 27.0 41.0 25 35.677254758043276 40.0 35.0 41.0 27.0 41.0 26 35.59022094569798 40.0 35.0 41.0 27.0 41.0 27 35.53035183701476 40.0 35.0 41.0 26.0 41.0 28 35.46547732805639 40.0 35.0 41.0 26.0 41.0 29 35.37529895714188 40.0 35.0 41.0 25.0 41.0 30 35.21139351000956 39.0 35.0 41.0 25.0 41.0 31 35.078309218411626 39.0 35.0 41.0 25.0 41.0 32 34.95780017338643 39.0 35.0 41.0 24.0 41.0 33 34.82766623959096 39.0 34.0 41.0 24.0 41.0 34 34.78029619102583 39.0 34.0 41.0 24.0 41.0 35 34.68828187593957 39.0 34.0 41.0 24.0 41.0 36 34.55155607098984 39.0 34.0 41.0 22.0 41.0 37 34.45607054168569 39.0 34.0 41.0 22.0 41.0 38 34.34890906474405 39.0 34.0 41.0 21.0 41.0 39 34.24443353379168 39.0 34.0 41.0 19.0 41.0 40 34.130475030013976 39.0 33.0 41.0 18.0 41.0 41 34.04502370494231 39.0 33.0 41.0 16.0 41.0 42 33.898112318752176 39.0 33.0 41.0 15.0 41.0 43 33.92671112038406 39.0 33.0 41.0 15.0 41.0 44 33.811345407973484 39.0 33.0 41.0 9.0 41.0 45 33.67393492083331 39.0 33.0 41.0 2.0 41.0 46 33.44138846576913 39.0 33.0 41.0 2.0 41.0 47 33.212573155179854 39.0 33.0 41.0 2.0 41.0 48 32.99946071940927 39.0 33.0 41.0 2.0 41.0 49 32.83014055653956 39.0 33.0 41.0 2.0 41.0 50 32.68149281631446 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7085085117141894 1101 2 0.7665985125108712 1101 3 0.7580962175456243 1101 4 0.6978851497548391 1101 5 0.8260773654586373 1101 6 0.7269164364772323 1101 7 0.8776128799613687 1101 8 0.7688330330655972 1101 9 0.8778283591518985 1101 10 0.9335394117471196 1101 11 0.8839263501818451 1101 12 0.8453799114377887 1101 13 0.9064701892461215 1101 14 1.0823020894144832 1101 15 1.049590171112925 1101 16 1.0418600085019136 1101 17 1.1487494597371821 1101 18 1.047481767451572 1101 19 1.0552035501026253 1101 20 0.933207901176516 1101 21 1.0604170170185299 1101 22 0.9613624755852683 1101 23 1.1553943845403012 1101 24 1.050006394486772 1101 25 1.0186605539820661 1101 26 0.9977018029257891 1101 27 0.957067482817088 1101 28 1.0380972645310393 1101 29 1.0126994137679688 1101 30 1.0579134214004497 1101 31 1.121959023676439 1101 32 1.1134415473159578 1101 33 1.1327799297881214 1101 34 1.105628605246558 1101 35 1.1125849971163646 1101 36 1.1370229666816556 1101 37 1.239244240012816 1101 38 1.177194021639707 1101 39 1.1757894792177197 1101 40 1.1833109121699579 1101 41 1.078570728723946 1101 42 1.1591462749517234 1101 43 1.2489117123465263 1101 44 1.249392747058124 1101 45 1.1557190034800229 1101 46 1.1723337717623323 1101 47 1.250118842058015 1101 48 1.3128447534792897 1101 49 1.2303595315176707 1101 50 1.4398015265318236 1102 1 0.5866297098892161 1102 2 0.7084931551720928 1102 3 0.616389548276846 1102 4 0.670260415912928 1102 5 0.5607858367863798 1102 6 0.5076644317351722 1102 7 0.5583361648740777 1102 8 0.5043437410436837 1102 9 0.6376081215247922 1102 10 0.5697083421532696 1102 11 0.7163269772129297 1102 12 0.6838260773534373 1102 13 0.6965525295536992 1102 14 0.7643178127673664 1102 15 0.7940347126510687 1102 16 0.8031437522017413 1102 17 0.8399678448165986 1102 18 0.7695405081657753 1102 19 0.847037096556619 1102 20 0.7563979776183771 1102 21 0.809864432530027 1102 22 0.8106452687378365 1102 23 0.736142962360411 1102 24 0.8308273313826717 1102 25 0.7025816197212933 1102 26 0.6213860619989475 1102 27 0.6768802036445578 1102 28 0.7508955727705029 1102 29 0.8894464934323736 1102 30 0.911707187596889 1102 31 0.829964301689671 1102 32 0.8551442842901125 1102 33 0.7548739155134072 1102 34 0.9083110370163894 1102 35 0.8542383376734648 1102 36 0.7988895638292348 1102 37 0.929385249185259 1102 38 0.8571316971972465 1102 39 0.9491322597675236 1102 40 1.0023938039697171 1102 41 1.0838329642285842 1102 42 0.8531457875413722 1102 43 0.9217640969191123 1102 44 0.9742476648052047 1102 45 0.8701304020855574 1102 46 0.9702933235001652 1102 47 1.2138433027753592 1102 48 1.340217043715647 1102 49 1.324189906891604 1102 50 1.1062277649376213 1103 1 0.5608660397188387 1103 2 0.7005500367415536 1103 3 0.649126807791891 1103 4 0.64990789437838 1103 5 0.6937109307035811 1103 6 0.6340750870685312 1103 7 0.686538836296684 1103 8 0.6743357723114727 1103 9 0.6616575656108523 1103 10 0.7430026539963279 1103 11 0.6961689306907104 1103 12 0.8163973216140548 1103 13 0.6924562217329537 1103 14 0.901737651115937 1103 15 0.8733283414599313 1103 16 0.8707774181980241 1103 17 0.8844343192217892 1103 18 0.8820950560350838 1103 19 0.9190061323400229 1103 20 0.9055071871960365 1103 21 0.875290569971682 1103 22 0.8133470484213632 1103 23 0.8348907330127062 1103 24 0.8237512869095553 1103 25 0.8054902069789094 1103 26 0.9118349672469321 1103 27 0.9831622208721953 1103 28 0.9383381789254059 1103 29 0.9391969962214333 1103 30 0.9840746599909238 1103 31 0.8322837719587071 1103 32 0.8293369758242832 1103 33 0.8469061319593578 1103 34 0.8601182316431917 1103 35 0.9298350439308152 1103 36 0.9439166954650986 1103 37 0.9661182587097912 1103 38 0.8775582141023435 1103 39 0.8529297401302856 1103 40 0.876835992714831 1103 41 0.882985812101694 1103 42 0.7467698531126601 1103 43 0.8304405284449388 1103 44 0.8535270637351147 1103 45 0.8697869876735354 1103 46 0.7901515939058115 1103 47 0.7925829149637238 1103 48 0.8666552101483092 1103 49 0.8975591627637982 1103 50 1.0451910708231011 1104 1 0.26914075278807204 1104 2 0.47675792270971584 1104 3 0.323090154700445 1104 4 0.2833630961554974 1104 5 0.44611281889314114 1104 6 0.3635826768500934 1104 7 0.31206641867358087 1104 8 0.3191571347698243 1104 9 0.3684374619455397 1104 10 0.4410913248589665 1104 11 0.4449795563848937 1104 12 0.3819656296481142 1104 13 0.4859401165867112 1104 14 0.6709388728856567 1104 15 0.5834634765950639 1104 16 0.6064725311191665 1104 17 0.46222958023622596 1104 18 0.38980516340272686 1104 19 0.317927828008294 1104 20 0.4874444326606806 1104 21 0.4183440686758644 1104 22 0.413601583778636 1104 23 0.3897824397879788 1104 24 0.39983421915687245 1104 25 0.3842064231951241 1104 26 0.2843081253365298 1104 27 0.4426818432342827 1104 28 0.40114824926971693 1104 29 0.4259704134742961 1104 30 0.5426039084174334 1104 31 0.5637443421179071 1104 32 0.5059706300264324 1104 33 0.5830501526924152 1104 34 0.43656214130627546 1104 35 0.5423026485217122 1104 36 0.7288991093858712 1104 37 0.6571169628934257 1104 38 0.6575378512737586 1104 39 0.7187942347619085 1104 40 0.6636556891235728 1104 41 0.7249424948117991 1104 42 0.7529296087587127 1104 43 0.637137122299066 1104 44 0.7743711955381514 1104 45 0.6844019450967167 1104 46 0.7592186061235111 1104 47 0.7780558879367021 1104 48 0.7640172982638234 1104 49 0.7421074782025272 1104 50 0.7954132107712653 1105 1 0.28657368055089094 1105 2 0.41330080931508206 1105 3 0.2857600305076069 1105 4 0.28045787087022944 1105 5 0.2980917763577722 1105 6 0.29896795382983754 1105 7 0.2933073563790316 1105 8 0.29752279216330635 1105 9 0.5338963855475853 1105 10 0.44282554408319186 1105 11 0.5237417032368441 1105 12 0.43408857064976303 1105 13 0.6025253539400239 1105 14 0.5028846432144647 1105 15 0.4545929199337735 1105 16 0.5567925820931663 1105 17 0.6284878995352017 1105 18 0.6268432678183729 1105 19 0.6480503322186024 1105 20 0.6157060706732338 1105 21 0.6437636360272876 1105 22 0.6247018251396241 1105 23 0.5251176208537487 1105 24 0.5511308590156574 1105 25 0.5204102336366887 1105 26 0.5417930177597725 1105 27 0.572236068402475 1105 28 0.6622330678437081 1105 29 0.747042668668513 1105 30 0.7532430422196796 1105 31 0.7417471700041105 1105 32 0.7773016953469991 1105 33 0.7370251790349869 1105 34 0.5984903724364372 1105 35 0.6483058276421829 1105 36 0.6985183579246481 1105 37 0.7246141710312486 1105 38 0.7446684924113995 1105 39 0.6865741189446553 1105 40 0.7495191850753713 1105 41 0.6715274169548735 1105 42 0.8486597482617668 1105 43 0.7654086669193276 1105 44 0.8564226738741993 1105 45 0.871762014262849 1105 46 0.8116736436335259 1105 47 0.7862350309250701 1105 48 0.7675177080371469 1105 49 0.6526316728403501 1105 50 0.7704681474140074 1106 1 0.3447121384314009 1106 2 0.55286688811724 1106 3 0.4924602605813426 1106 4 0.32752219148365924 1106 5 0.2165454899553083 1106 6 0.30800625346791577 1106 7 0.4069075973277094 1106 8 0.32775819004353934 1106 9 0.5231148257056404 1106 10 0.3858187124820418 1106 11 0.4005522235582859 1106 12 0.32088834896651264 1106 13 0.4771545651752618 1106 14 0.4878013779675854 1106 15 0.5364251367509709 1106 16 0.5161235572270257 1106 17 0.4950442621516302 1106 18 0.46472606206463496 1106 19 0.4447871513604227 1106 20 0.45714000298574575 1106 21 0.5240519593657709 1106 22 0.33162674595847363 1106 23 0.49344341763960387 1106 24 0.47836505542151997 1106 25 0.43156934366955824 1106 26 0.44515605632834365 1106 27 0.45658092053264454 1106 28 0.4583075832276151 1106 29 0.416365124732323 1106 30 0.5464686053576671 1106 31 0.5498525562836747 1106 32 0.4864929198113117 1106 33 0.6156774660751267 1106 34 0.5953521117868945 1106 35 0.5117576005306219 1106 36 0.5505191843332824 1106 37 0.5261832577690697 1106 38 0.483886551183609 1106 39 0.6574288307706055 1106 40 0.680973579283183 1106 41 0.5923094366391766 1106 42 0.6454629285402333 1106 43 0.5884475972606182 1106 44 0.582879671312817 1106 45 0.6673991345687256 1106 46 0.5890764888590923 1106 47 0.6127595009676483 1106 48 0.6166426672561016 1106 49 0.6212437269430922 1106 50 0.6829669024616507 1107 1 0.4453014388317236 1107 2 0.564348422832726 1107 3 0.3419203307802938 1107 4 0.45199074526104255 1107 5 0.41098251628884697 1107 6 0.348228408407401 1107 7 0.46452661619229474 1107 8 0.3707979986506089 1107 9 0.40335689047680745 1107 10 0.48599212618044874 1107 11 0.5737924232179168 1107 12 0.47446642042588394 1107 13 0.41874948642469434 1107 14 0.5977255913336208 1107 15 0.559771774489839 1107 16 0.5336397015405652 1107 17 0.5211926069820905 1107 18 0.4464336384317207 1107 19 0.41486180685059537 1107 20 0.5752677729059954 1107 21 0.48111293311898606 1107 22 0.47347005107558005 1107 23 0.6464218978416056 1107 24 0.4290833317284779 1107 25 0.4423887765497838 1107 26 0.5289448947457274 1107 27 0.47792636929480636 1107 28 0.4748724611278732 1107 29 0.45051439829136797 1107 30 0.5409217483640134 1107 31 0.5518158987200223 1107 32 0.488848973259131 1107 33 0.5757249145804408 1107 34 0.49759173396785883 1107 35 0.42408927910548044 1107 36 0.5096959497893465 1107 37 0.4915698325381044 1107 38 0.47674057154929983 1107 39 0.4827866081495955 1107 40 0.5361814333333186 1107 41 0.39067370066004514 1107 42 0.43653195297549274 1107 43 0.5032316466023019 1107 44 0.5003491240518372 1107 45 0.5275942271056451 1107 46 0.6649596527618229 1107 47 0.608317635379855 1107 48 0.6226200818015855 1107 49 0.627879628766614 1107 50 0.7287620667794172 1108 1 0.29580420716627387 1108 2 0.41217833096237655 1108 3 0.3421875746487544 1108 4 0.3028213510067843 1108 5 0.33230965186874073 1108 6 0.299768011293132 1108 7 0.25515853834593116 1108 8 0.3410760755907063 1108 9 0.35129534008299856 1108 10 0.300146145481861 1108 11 0.3735928333571721 1108 12 0.33113445000817876 1108 13 0.334373030862416 1108 14 0.4502617735821346 1108 15 0.28106740142751363 1108 16 0.30245078246613843 1108 17 0.3858541367213988 1108 18 0.26913408714008114 1108 19 0.4484903800254614 1108 20 0.36428474542076117 1108 21 0.39013926338434857 1108 22 0.41492172482730183 1108 23 0.45595320942309314 1108 24 0.3025511581379874 1108 25 0.4283022004959278 1108 26 0.4186087955367128 1108 27 0.3980680844187461 1108 28 0.4788684735458588 1108 29 0.482026307293566 1108 30 0.6472487680258254 1108 31 0.5168084272727569 1108 32 0.4854786023423472 1108 33 0.5040596143420757 1108 34 0.5387665742643577 1108 35 0.5059223865741842 1108 36 0.5421372056669185 1108 37 0.4731179922858644 1108 38 0.5821129531179068 1108 39 0.5544144984615826 1108 40 0.5396215658592993 1108 41 0.4421158269770302 1108 42 0.4769029540651246 1108 43 0.4512721041204344 1108 44 0.5304336599474553 1108 45 0.599794647361378 1108 46 0.7118074185988661 1108 47 0.5626801596963062 1108 48 0.675825743662223 1108 49 0.6728217013397639 1108 50 0.6794981465985046 1109 1 0.14694640735428877 1109 2 0.239965608628669 1109 3 0.17836838427444945 1109 4 0.20689884381096135 1109 5 0.21504860807541348 1109 6 0.22637552731900001 1109 7 0.10938274908850332 1109 8 0.1284725481345319 1109 9 0.20523929258540363 1109 10 0.20927876646190668 1109 11 0.10165715267516617 1109 12 0.1856416167748023 1109 13 0.2127292296434291 1109 14 0.26718632071761306 1109 15 0.24896854632548582 1109 16 0.1876639177682975 1109 17 0.3174680132915668 1109 18 0.33775823811636485 1109 19 0.3290643646730018 1109 20 0.31159702635552833 1109 21 0.17792681082902817 1109 22 0.31054374040389376 1109 23 0.32754284744275 1109 24 0.2363146496513835 1109 25 0.3016169675422944 1109 26 0.26281281440459736 1109 27 0.18409314890615747 1109 28 0.11435636775952673 1109 29 0.17601886141007128 1109 30 0.3660713098777961 1109 31 0.266607961178714 1109 32 0.37762815502902924 1109 33 0.23712064443896708 1109 34 0.12247330855547034 1109 35 0.24059399364404044 1109 36 0.2709315868056734 1109 37 0.2093693593359589 1109 38 0.08100655044668059 1109 39 0.1378818563143156 1109 40 0.19557163195500493 1109 41 0.13749224046014064 1109 42 0.03594644441639616 1109 43 0.0029410984903606163 1109 44 -0.030703725733950193 1109 45 -0.03932716173098072 1109 46 -0.09812125614593015 1109 47 0.08317190344003933 1109 48 0.04283257320585676 1109 49 0.011932200492033473 1109 50 0.06031700701309006 1110 1 -0.03193339828607833 1110 2 0.03833103428282669 1110 3 0.022734991845283048 1110 4 0.012451800244200228 1110 5 -0.056433958653634875 1110 6 -0.04742737165994981 1110 7 0.035195316802479226 1110 8 -0.06144750833482249 1110 9 0.016575022583104726 1110 10 0.051243785011429566 1110 11 0.0793090782144219 1110 12 -0.014240191557711057 1110 13 -0.15191504626844932 1110 14 0.08544857489535929 1110 15 -0.0616075559759679 1110 16 0.059331158977791176 1110 17 0.13198876249541058 1110 18 0.11321992431749095 1110 19 0.301391098971564 1110 20 0.16501719773344092 1110 21 0.19874947408127497 1110 22 0.09340061357766416 1110 23 0.06450523951077969 1110 24 0.02760622192887041 1110 25 -0.0352149212262205 1110 26 -0.07130518027678079 1110 27 -0.11559085253841062 1110 28 -0.03487046174899433 1110 29 -0.04918551168162821 1110 30 -0.041171212857690875 1110 31 0.016776220149786525 1110 32 -0.015157228601431427 1110 33 -0.08676966712740608 1110 34 -0.11251963277549493 1110 35 -0.048523754804691066 1110 36 -0.11193595644919441 1110 37 -0.0458109650345051 1110 38 -0.08888453264862761 1110 39 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2214 2 -1.8339683502691102 2214 3 -0.8396640857757269 2214 4 -0.532078068021363 2214 5 -0.4952211651063223 2214 6 -0.3522751156779691 2214 7 -0.38832356301859505 2214 8 -0.47522271910199265 2214 9 -0.6097974424608736 2214 10 -0.45979512066480765 2214 11 -0.36701488857042364 2214 12 -0.40167624761424037 2214 13 -0.5330776562123773 2214 14 -0.6460233452867428 2214 15 -0.6446503161336352 2214 16 -0.5701922058417352 2214 17 -0.49178163016996734 2214 18 -0.5208877751281307 2214 19 -0.5657605502864911 2214 20 -0.4892947059521049 2214 21 -0.7070384937504599 2214 22 -0.6668739389138665 2214 23 -0.5548551029511728 2214 24 -0.6434748570086839 2214 25 -0.6567198146728046 2214 26 -0.6959833786636054 2214 27 -0.6638394824526728 2214 28 -0.7575072125639011 2214 29 -0.7124358864228739 2214 30 -0.6062425524882613 2214 31 -0.6973745249706482 2214 32 -0.6320027400340109 2214 33 -0.5422161049044973 2214 34 -0.6363111418129108 2214 35 -0.7209615358606314 2214 36 -0.6081594965445234 2214 37 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-1.1732362350204326 2216 10 -1.2504504581271974 2216 11 -1.3973214989269422 2216 12 -1.245083638689465 2216 13 -1.3130897021082575 2216 14 -1.378904287527881 2216 15 -1.4034877765877383 2216 16 -1.4514334878396369 2216 17 -1.3881147766064217 2216 18 -1.4196328216879266 2216 19 -1.3933933825325582 2216 20 -1.4120390320753415 2216 21 -1.3906517744998865 2216 22 -1.4673633056283677 2216 23 -1.3933045774221995 2216 24 -1.4435861876290303 2216 25 -1.550383799831529 2216 26 -1.6841751706723116 2216 27 -1.6591507640608611 2216 28 -1.701542949306834 2216 29 -1.6234237025486493 2216 30 -1.7327745773327905 2216 31 -1.62967376384276 2216 32 -1.597105189834295 2216 33 -1.6250311124546855 2216 34 -1.5044285940374635 2216 35 -1.6175962685565466 2216 36 -1.7254720750789545 2216 37 -1.8413510709747243 2216 38 -1.8838010756213563 2216 39 -1.927212299581747 2216 40 -1.9493294015289422 2216 41 -2.197038055545594 2216 42 -2.051267479930427 2216 43 -1.804885533160025 2216 44 -1.7654714280161627 2216 45 -1.597996741899017 2216 46 -1.604735131950548 2216 47 -1.6353966537082378 2216 48 -1.6525570797464226 2216 49 -1.6745374584480857 2216 50 -1.7661515037730169 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45826.0 3 7605.0 4 13496.0 5 7815.0 6 6018.0 7 4500.0 8 3781.0 9 3428.0 10 3323.0 11 3230.0 12 3431.0 13 3740.0 14 4192.0 15 4479.0 16 5013.0 17 5423.0 18 5710.0 19 6491.0 20 7783.0 21 10243.0 22 13303.0 23 16651.0 24 19546.0 25 21375.0 26 21894.0 27 24515.0 28 28912.0 29 33722.0 30 37682.0 31 43437.0 32 53870.0 33 67110.0 34 83619.0 35 103294.0 36 130848.0 37 171117.0 38 264728.0 39 717081.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.250918098721385 19.206814498738517 14.410290275574042 24.131977126966056 2 14.035947346275174 21.59903197615793 39.80983111868002 24.555189558886873 3 17.12636781237162 27.75311531327416 30.08627021374597 25.03424666060825 4 10.944704820824976 17.35620484771467 38.636911389480346 33.062178941979994 5 11.883911563812575 40.04350676262662 34.210940551052445 13.861641122508347 6 29.2473495311686 38.96077116863686 18.52593788693657 13.265941413257973 7 26.394652154444394 34.03120107865419 22.167150556834805 17.40699621006661 8 26.341295048303703 34.220748205638266 20.655318686912203 18.782638059145828 9 27.396842695633026 14.960585995052952 19.943695701766533 37.698875607547485 10 14.13993595487653 30.272851751328794 33.82516946455228 21.762042829242393 11 33.16828674669268 24.307128119654458 24.314550234767975 18.21003489888489 12 23.105891166886007 26.983124401623105 30.687706287477944 19.22327814401294 13 27.781663095163378 23.04785216666218 27.04360222718347 22.126882510990963 14 21.86352093045035 21.88025851599386 29.617356876208618 26.638863677347175 15 22.83275044544722 29.656150110608394 25.885004131937063 21.626095312007322 16 21.849157266289904 29.03781155741972 27.274185473646984 21.838845702643397 17 20.41151718067292 28.35930638671596 28.369618227748216 22.859558204862903 18 20.245141434073403 27.325837143948856 31.00126486364232 21.42775655833542 19 21.569297556460366 26.88043051014476 31.503871133910195 20.04640079948468 20 22.09294331798658 26.219678047040563 31.277729013128596 20.40964962184426 21 22.723507026417376 25.991542095485947 30.79264085589331 20.49231002220337 22 21.69380883563906 26.392191264988966 30.777082514485205 21.136917384886765 23 21.34993108072497 26.314958347053256 30.778247313343837 21.556863258877932 24 21.03981887604516 26.310197188992085 31.32164197134197 21.328341963620787 25 21.201523086901034 26.272302592973755 31.320616368003616 21.20555795212159 26 20.994290741297004 26.520978698751296 31.353748379155356 21.130982180796348 27 21.115298302070542 26.860860446986095 30.83790589530473 21.185935355638634 28 20.912393160264337 26.385317463084935 31.240242576886253 21.46204679976448 29 21.0074401589253 26.628854825243938 31.385057116270914 20.978647899559846 30 21.246785876886506 26.64516778195867 31.040484252913757 21.067562088241065 31 21.581659464777157 26.18034195484602 30.915258458588095 21.322740121788726 32 20.90028774978868 26.873548985454114 30.84655818478049 21.37960507997671 33 21.202332107114362 26.323564422317386 30.946091190448442 21.528012280119814 34 21.297817629987705 26.447770589925707 31.204519431853665 21.04989234823292 35 21.57503562262775 26.608870270881386 30.650949048560825 21.165145057930037 36 21.248041406143386 26.534327314527513 30.536034640589932 21.68159663873917 37 21.568386248195264 26.254052096930593 30.497118647191034 21.68044300768311 38 21.18253784454787 26.601822315521055 30.92398220017581 21.291657639755265 39 21.513002364066196 26.80896880996384 30.314978079971873 21.363050745998095 40 21.70482800382651 26.246540879250357 30.810264643590422 21.23836647333271 41 21.428244182815416 26.561150778197522 30.361156953182654 21.64944808580441 42 21.676768189837922 26.582219227702826 30.008520436847924 21.732492145611328 43 21.28842390411266 26.412690034309506 30.35265587419517 21.946230187382668 44 21.474989094516182 26.877934272300468 29.760716754209316 21.88635987897403 45 21.82788663212807 26.562702296796914 29.52831109437961 22.08109997669541 46 21.816616431391626 26.71548941175182 29.216613543257523 22.25128061359903 47 21.858766751119397 26.922544497028223 28.92859561483181 22.290093137020573 48 21.772742256583186 27.118017828706186 28.826544077298227 22.282695837412398 49 22.04876931427177 26.99924809409281 28.5583324619192 22.393650129716217 50 22.112102920687214 27.040084133957038 28.503216271475424 22.34459667388033 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1570.0 1 1048.5 2 527.0 3 921.5 4 1316.0 5 1383.5 6 1451.0 7 1295.5 8 1140.0 9 1562.5 10 1985.0 11 2922.0 12 3859.0 13 7469.0 14 11079.0 15 15248.5 16 19418.0 17 21671.0 18 23924.0 19 24149.0 20 24374.0 21 24809.5 22 25245.0 23 27387.0 24 29529.0 25 33725.5 26 37922.0 27 43676.0 28 49430.0 29 54148.0 30 58866.0 31 64519.0 32 70172.0 33 78074.5 34 85977.0 35 92710.0 36 99443.0 37 105868.5 38 112294.0 39 117515.5 40 122737.0 41 126609.0 42 130481.0 43 130070.0 44 129659.0 45 130347.5 46 131036.0 47 131444.0 48 131852.0 49 129484.0 50 127116.0 51 122381.0 52 117646.0 53 111199.5 54 104753.0 55 97483.0 56 90213.0 57 82752.0 58 75291.0 59 66820.5 60 58350.0 61 50908.0 62 43466.0 63 36984.5 64 30503.0 65 25090.0 66 19677.0 67 15895.5 68 12114.0 69 10496.0 70 8878.0 71 7135.0 72 5392.0 73 4816.0 74 4240.0 75 3020.5 76 1801.0 77 1542.5 78 1284.0 79 1096.0 80 908.0 81 719.5 82 531.0 83 451.5 84 372.0 85 306.0 86 240.0 87 162.0 88 84.0 89 64.5 90 45.0 91 33.5 92 22.0 93 16.0 94 10.0 95 8.0 96 6.0 97 4.5 98 3.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013793233945696485 2 7.967210943362592E-4 3 2.9877041037609716E-4 4 0.0013444668466924372 5 0.036201014723903774 6 0.02355306735131566 7 0.02708851720743281 8 0.020615158315950705 9 0.02833339391733321 10 0.029080319943273456 11 0.03570306403994361 12 0.03570306403994361 13 0.0335120810305189 14 0.03849158787012052 15 0.0347569577404193 16 0.03864097307530857 17 0.04132990676869344 18 0.04481556155641458 19 0.04670777415546319 20 0.042674373615385876 21 0.04372007005170222 22 0.04172826731586157 23 0.04033400540077312 24 0.038143022391348404 25 0.036300604860695806 26 0.039338104032852796 27 0.03913892375926873 28 0.039089128690872714 29 0.037196916091824093 30 0.034109621851271095 31 0.03266556486778662 32 0.029080319943273456 33 0.02156126461547501 34 0.01832458516973396 35 0.018822535853694124 36 0.020565363247554688 37 0.015934421886725184 38 0.017229093665021605 39 0.01244876709900405 40 0.00667253916506617 41 0.006423563823086088 42 0.005875818070729911 43 0.002041597804236664 44 0.003336269582533085 45 0.0021411879410286965 46 6.971309575442268E-4 47 3.485654787721134E-4 48 4.4815561556414577E-4 49 4.97950683960162E-5 50 2.48975341980081E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2008231.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.27390705412618 #Duplication Level Percentage of deduplicated Percentage of total 1 72.20995894388446 38.46906641158767 2 13.980391311831685 14.895801346536686 3 5.616601329380665 8.976548915445212 4 2.9034290320100675 6.187080335582236 5 1.6088341510368624 4.28544405139209 6 1.0232108355656602 3.2706263370419855 7 0.6459824071424237 2.4089804701694324 8 0.43340388878539016 1.8471294790439767 9 0.30410510876719427 1.4580780569233593 >10 1.1557045576191352 10.14391924985103 >50 0.07596530201431968 2.814177098842037 >100 0.04015209362514684 3.747858107265246 >500 0.0019784085449283356 0.6940177914386408 >1k 1.8841986142174448E-4 0.435241704321366 >5k 9.420993071087224E-5 0.3660306445590086 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7293 0.36315543381214616 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4373 0.2177538340957788 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4299 0.21406899903447363 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.979506839601619E-5 0.0 3 0.0 0.0 0.0 4.979506839601619E-5 0.0 4 0.0 0.0 0.0 4.979506839601619E-5 0.0 5 0.0 0.0 0.0 4.979506839601619E-5 0.0 6 0.0 0.0 0.0 4.979506839601619E-5 0.0 7 0.0 0.0 0.0 4.979506839601619E-5 0.0 8 0.0 0.0 0.0 4.979506839601619E-5 0.0 9 0.0 0.0 0.0 4.979506839601619E-5 0.0 10 2.9877041037609716E-4 0.0 0.0 9.959013679203239E-5 0.0 11 2.9877041037609716E-4 0.0 0.0 9.959013679203239E-5 0.0 12 2.9877041037609716E-4 0.0 0.0 9.959013679203239E-5 0.0 13 2.9877041037609716E-4 0.0 0.0 9.959013679203239E-5 0.0 14 2.9877041037609716E-4 0.0 0.0 9.959013679203239E-5 0.0 15 2.9877041037609716E-4 0.0 0.0 9.959013679203239E-5 0.0 16 5.975408207521943E-4 0.0 0.0 1.4938520518804858E-4 0.0 17 5.975408207521943E-4 0.0 0.0 1.4938520518804858E-4 0.0 18 5.975408207521943E-4 0.0 0.0 1.4938520518804858E-4 0.0 19 5.975408207521943E-4 0.0 0.0 1.4938520518804858E-4 0.0 20 5.975408207521943E-4 0.0 0.0 1.9918027358406477E-4 0.0 21 6.971309575442268E-4 0.0 0.0 2.48975341980081E-4 0.0 22 6.971309575442268E-4 0.0 0.0 4.4815561556414577E-4 0.0 23 8.963112311282915E-4 0.0 0.0 4.97950683960162E-4 0.0 24 8.963112311282915E-4 0.0 0.0 9.461062995243078E-4 0.0 25 8.963112311282915E-4 0.0 0.0 9.461062995243078E-4 0.0 26 8.963112311282915E-4 0.0 0.0 0.0015934421886725182 0.0 27 8.963112311282915E-4 0.0 0.0 0.0018922125990486155 0.0 28 0.00104569643631634 0.0 0.0 0.0027387287617808907 0.0 29 0.00104569643631634 0.0 0.0 0.00458114629243349 0.0 30 0.0010954915047123562 0.0 0.0 0.0067721293018582025 0.0 31 0.0010954915047123562 0.0 0.0 0.01165204600466779 0.0 32 0.0010954915047123562 0.0 0.0 0.015486266271161037 0.0 33 0.001244876709900405 0.0 0.0 0.022059215299435175 0.0 34 0.0013444668466924372 0.0 0.0 0.029130115011669476 0.0 35 0.0013444668466924372 0.0 0.0 0.037595276638992224 0.0 36 0.0013444668466924372 0.0 0.0 0.04690695442904726 0.0 37 0.0013444668466924372 0.0 0.0 0.06060059823795171 0.0 38 0.0013444668466924372 0.0 0.0 0.07663461026146892 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3455 0.0 27.830702 1 GCGTATC 75 7.006565E-8 26.404139 1 GTATCAA 8050 0.0 24.8188 1 TTCGCGT 50 0.0025865205 21.991945 43 CGTATCA 115 2.0213156E-7 19.132004 2 CGTTACG 75 0.0012903479 17.601883 24 CCGTATT 165 1.6589183E-9 17.33519 27 TATCAAC 11670 0.0 17.00568 2 TCGGCGT 105 3.7720798E-5 16.764116 13 GTCGGCG 105 3.7720798E-5 16.764116 12 ATCAACG 12025 0.0 16.632133 3 TAACCGT 175 3.6379788E-9 16.340128 33 TCAACGC 12215 0.0 16.337402 4 CAACGCA 12275 0.0 16.222097 5 CGCACTG 95 3.34433E-4 16.212664 5 TATATCG 95 3.3449646E-4 16.212261 4 GTACATG 10615 0.0 16.043966 1 AACGCAG 12580 0.0 15.932944 6 CGAAATC 170 4.487447E-8 15.53146 13 CGTATTA 185 7.565177E-9 15.461114 28 >>END_MODULE