FastQCFastQC Report
Mon 6 Feb 2017
SRR2077418_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077418_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3016667
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46140.1529502593425128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68500.022.4835341
GGTATCA32750.018.810651
TATCAAC86800.017.514342
ATCAACG87250.017.2727153
TCAACGC87250.017.0964874
CAACGCA88550.016.794695
AACGCAG89800.016.5358646
GTACATG96300.015.6274061
TACATGG96250.015.06332
AGAGTAC92750.014.49314811
ACGCAGA102050.014.335587
ACATGGG101950.014.0053173
CTATGCG1100.001018648213.9999479
GTATAGA6950.013.9291611
CGCAGAG103500.013.922198
CGTGCCG1751.0539534E-613.82874910
GAGTACT50400.013.75040412
ATCGTCG1302.3363324E-413.53571544
AGTACTT52650.013.45528713
GGACCGT2155.4786142E-813.3016146
Produced by FastQC (version 0.11.5)