##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077418_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3016667 Sequences flagged as poor quality 0 Sequence length 50 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.391786697040143 31.0 28.0 33.0 10.0 34.0 2 27.436469786025437 31.0 27.0 34.0 10.0 34.0 3 27.52035408614872 31.0 27.0 34.0 10.0 34.0 4 30.747233619090206 35.0 32.0 37.0 10.0 37.0 5 30.81034598780707 35.0 32.0 37.0 7.0 37.0 6 30.821799025215576 35.0 32.0 37.0 8.0 37.0 7 30.6087725294174 35.0 31.0 37.0 7.0 37.0 8 30.498570442146914 35.0 31.0 37.0 2.0 37.0 9 31.529812206650586 37.0 31.0 39.0 2.0 39.0 10 31.416530893201006 37.0 31.0 39.0 2.0 39.0 11 31.466362710899148 37.0 31.0 39.0 2.0 39.0 12 31.37627222361633 37.0 31.0 39.0 2.0 39.0 13 31.375584378388467 37.0 31.0 39.0 2.0 39.0 14 32.05886264542954 38.0 31.0 40.0 2.0 41.0 15 32.153764734390634 38.0 31.0 40.0 2.0 41.0 16 32.05145016006075 38.0 31.0 40.0 2.0 41.0 17 31.865940788293834 38.0 30.0 40.0 2.0 41.0 18 31.66668081031151 38.0 30.0 40.0 2.0 41.0 19 32.033294360961946 38.0 31.0 40.0 2.0 41.0 20 32.06682971637241 38.0 31.0 40.0 2.0 41.0 21 32.07084905294486 38.0 31.0 40.0 2.0 41.0 22 31.95551083364521 38.0 30.0 40.0 2.0 41.0 23 31.68598091867614 38.0 30.0 40.0 2.0 41.0 24 31.84390355315983 38.0 30.0 40.0 2.0 41.0 25 31.732241576547892 38.0 30.0 40.0 2.0 41.0 26 31.76589792642012 38.0 30.0 40.0 2.0 41.0 27 31.730459145805618 38.0 30.0 40.0 2.0 41.0 28 31.60284877316588 38.0 30.0 40.0 2.0 41.0 29 31.560233529255964 38.0 30.0 40.0 2.0 41.0 30 31.322870903550175 38.0 30.0 40.0 2.0 41.0 31 31.244247376326257 38.0 30.0 40.0 2.0 41.0 32 31.24696196166166 38.0 30.0 40.0 2.0 41.0 33 31.199287491791438 38.0 30.0 40.0 2.0 41.0 34 31.173871693494842 38.0 30.0 40.0 2.0 41.0 35 31.102720651633078 38.0 30.0 40.0 2.0 41.0 36 30.975072488942267 38.0 30.0 40.0 2.0 41.0 37 30.872727417378186 38.0 29.0 40.0 2.0 41.0 38 30.75682135283742 37.0 29.0 40.0 2.0 41.0 39 30.6851236812018 37.0 29.0 40.0 2.0 41.0 40 30.650908767855384 37.0 29.0 40.0 2.0 41.0 41 30.5219313235435 37.0 29.0 40.0 2.0 41.0 42 30.33260283617648 37.0 28.0 40.0 2.0 41.0 43 30.226793676597385 37.0 28.0 40.0 2.0 41.0 44 30.153115673688877 37.0 28.0 40.0 2.0 41.0 45 30.048759773617704 37.0 28.0 40.0 2.0 41.0 46 29.876886974929615 37.0 27.0 40.0 2.0 41.0 47 29.675897273381516 37.0 27.0 40.0 2.0 41.0 48 29.47339000294033 37.0 26.0 40.0 2.0 41.0 49 29.326147035784857 37.0 26.0 40.0 2.0 41.0 50 29.106493358398524 36.0 26.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7279134976991308 1101 2 0.71393638002667 1101 3 0.7693962229311886 1101 4 0.7655092253255411 1101 5 0.7003912075323768 1101 6 0.7852907703545071 1101 7 0.7937669956463509 1101 8 0.8212573313419078 1101 9 1.0916481699161587 1101 10 1.0558355826808032 1101 11 1.006001688564357 1101 12 0.9933293104540795 1101 13 0.9993485442758399 1101 14 1.2366227487444377 1101 15 1.2605081806593716 1101 16 1.0854667825014275 1101 17 1.2742303283661087 1101 18 1.437014813157731 1101 19 1.1109477959082739 1101 20 1.1859152382943776 1101 21 1.165249287586036 1101 22 0.9660479919980958 1101 23 1.1049745616651698 1101 24 1.0308603374613732 1101 25 1.0154436093985773 1101 26 1.0301688799022024 1101 27 1.149799942035905 1101 28 1.246624600628767 1101 29 1.1621737167793675 1101 30 1.3477658554926144 1101 31 1.2461152713425818 1101 32 1.304697316096373 1101 33 1.2385000904878396 1101 34 1.1980433568094604 1101 35 1.175644223453677 1101 36 1.2668845874797299 1101 37 1.2929901768482566 1101 38 1.2384674850267814 1101 39 1.2165786341117233 1101 40 1.2737970020157512 1101 41 1.132844027517372 1101 42 1.2218944514728634 1101 43 1.3371578518546805 1101 44 1.0422935771554052 1101 45 1.1901975120705934 1101 46 1.197124886162893 1101 47 1.1426799087395203 1101 48 1.1683466815854224 1101 49 1.3347720235126275 1101 50 1.3862355177163863 1102 1 0.559047863222851 1102 2 0.5753706510899477 1102 3 0.48418995035043366 1102 4 0.483942098577959 1102 5 0.580717923542533 1102 6 0.5770538197718302 1102 7 0.6661002704192995 1102 8 0.6900783580836354 1102 9 0.8149722608935299 1102 10 0.9114389201133655 1102 11 0.6502710968753469 1102 12 0.5225838367897033 1102 13 0.6082803774449523 1102 14 0.9361657231535503 1102 15 0.9632694809035414 1102 16 1.1230582194459018 1102 17 1.112796006380755 1102 18 1.2222927840150248 1102 19 0.9638012905627278 1102 20 0.7815804460436979 1102 21 0.7062186910594264 1102 22 0.8802150724065925 1102 23 1.084660983549437 1102 24 0.9118419622319074 1102 25 0.9655363130295704 1102 26 0.7614333003963019 1102 27 0.8493070163846781 1102 28 0.862539428372127 1102 29 0.6791493021922328 1102 30 0.8536990406427201 1102 31 0.8884606910295645 1102 32 0.9083449659154752 1102 33 0.7062207931105817 1102 34 0.6259382638384849 1102 35 0.6972850344385861 1102 36 0.7916954501760038 1102 37 0.7480305707814026 1102 38 0.8285195528447922 1102 39 0.8460605274961814 1102 40 0.8261192266992055 1102 41 0.8396906527843946 1102 42 0.8691338386766532 1102 43 0.8568741140727951 1102 44 0.779708416356236 1102 45 0.760033212007194 1102 46 0.7483472959480757 1102 47 0.9683583816884429 1102 48 0.920363221074215 1102 49 0.8242017217738926 1102 50 0.8216504434847351 1103 1 0.3665638497477737 1103 2 0.345550285164947 1103 3 0.4321029271724939 1103 4 0.4448066821214205 1103 5 0.5279150873212011 1103 6 0.512999899290925 1103 7 0.593684338359509 1103 8 0.6979065105053159 1103 9 0.7339347001245606 1103 10 0.7144052525927371 1103 11 0.8076353879208789 1103 12 0.702023652818589 1103 13 0.6942397706630317 1103 14 0.9967612085992599 1103 15 0.8800638044264275 1103 16 1.031910820660272 1103 17 1.1307708112140347 1103 18 0.674428926673329 1103 19 0.6828188000941253 1103 20 0.795476215918427 1103 21 0.7767630397976504 1103 22 0.8447591790775633 1103 23 0.9900368938231772 1103 24 0.8659313859101196 1103 25 0.6602725605836817 1103 26 0.7010132128486397 1103 27 0.769060208646593 1103 28 0.9045246824418847 1103 29 0.8085131696200669 1103 30 0.9582352801279193 1103 31 0.954694023690486 1103 32 0.8612198171449705 1103 33 0.8674629007094765 1103 34 0.800575566402383 1103 35 0.6979886432579008 1103 36 0.7946792316699636 1103 37 0.7866942586760217 1103 38 0.6405519091677903 1103 39 0.8675464169924787 1103 40 0.7123727316313264 1103 41 0.7835373031787611 1103 42 0.7521558466245288 1103 43 0.8560737792130908 1103 44 0.8397081137721685 1103 45 0.7396301700287005 1103 46 0.6886965788238371 1103 47 0.7190820619176002 1103 48 0.6783687886210679 1103 49 0.6092478154135748 1103 50 0.7770820292269747 1104 1 0.6869452897470154 1104 2 0.6744446048666752 1104 3 0.7262989210836004 1104 4 0.7330314897517205 1104 5 0.7155221155985707 1104 6 0.8280979065077716 1104 7 0.7395649904096189 1104 8 0.9192621520277307 1104 9 1.148077203083563 1104 10 1.1036342661704062 1104 11 0.8909948020482368 1104 12 0.9247893838787036 1104 13 0.9491417594995326 1104 14 1.103943800073779 1104 15 1.1216308444332483 1104 16 1.1597648916123973 1104 17 1.3239588005249203 1104 18 1.4790212414362145 1104 19 1.2667345441979236 1104 20 1.1003697589189798 1104 21 1.1098684375156225 1104 22 1.174706366949657 1104 23 1.2326843792333868 1104 24 1.2056363756231683 1104 25 1.1708095302572126 1104 26 1.0730783835235904 1104 27 1.0075689877773257 1104 28 1.1973215387754514 1104 29 1.210111928871509 1104 30 1.3488457700140337 1104 31 1.3669701547714368 1104 32 1.1448080483067145 1104 33 1.105245791813843 1104 34 0.9960268621475343 1104 35 1.0482923184199997 1104 36 1.0703016384727277 1104 37 1.0511101462406351 1104 38 1.1551555093829613 1104 39 1.0188959944836178 1104 40 1.0230642028762134 1104 41 1.1134609591364502 1104 42 1.2299406340953283 1104 43 1.0847607220691167 1104 44 1.1468039874287754 1104 45 1.0578759777959483 1104 46 1.2109194379918904 1104 47 1.1327259053570593 1104 48 1.179013770275052 1104 49 1.1023351552238907 1104 50 1.2922268141403421 1105 1 0.39914449440238187 1105 2 0.3764166860659124 1105 3 0.41025540003439076 1105 4 0.36316108665388924 1105 5 0.36690516979967214 1105 6 0.34623722240829835 1105 7 0.4206344648809477 1105 8 0.4672595390876957 1105 9 0.6700097632282116 1105 10 0.6506556472359541 1105 11 0.6052238626110942 1105 12 0.6763176537704467 1105 13 0.7193286733742497 1105 14 0.9489116018288257 1105 15 0.8430715983354915 1105 16 0.8458402733745487 1105 17 0.810290870084593 1105 18 1.15287296430148 1105 19 0.8911462432301605 1105 20 0.7489246122925763 1105 21 0.7181086514435933 1105 22 0.7510608655588484 1105 23 1.1189026303098863 1105 24 0.8112128273906158 1105 25 0.8300323661914639 1105 26 0.794741370033659 1105 27 0.6767810399968965 1105 28 0.83711392678455 1105 29 0.6346151077305855 1105 30 0.950784376885796 1105 31 0.9782302703939365 1105 32 0.9874956338797318 1105 33 0.9842307609657226 1105 34 0.8127429469609595 1105 35 0.8119140906380196 1105 36 0.8083941633986491 1105 37 0.9104255101780225 1105 38 0.9376903727844343 1105 39 0.9177438564059095 1105 40 0.874448382183985 1105 41 0.9940812275580555 1105 42 1.0606262644829805 1105 43 1.0247405605422664 1105 44 1.0802973455490736 1105 45 1.0446006326526494 1105 46 0.997398088756281 1105 47 1.0701160271513004 1105 48 1.0954901785191424 1105 49 1.0525546664198586 1105 50 0.9378353304862195 1106 1 0.2832453677448754 1106 2 0.19331636741238967 1106 3 0.3311774826592604 1106 4 0.4057057119292047 1106 5 0.4293995294548658 1106 6 0.3685203131548853 1106 7 0.4216709667381551 1106 8 0.5139286488024553 1106 9 -1.0230733521376578 1106 10 -0.7056914256941269 1106 11 0.09846602154062367 1106 12 0.4191966594536467 1106 13 0.4988878561199144 1106 14 0.666077998325342 1106 15 0.5669715753398776 1106 16 0.6698098791375742 1106 17 0.7670764833632973 1106 18 -1.1262098807054315 1106 19 0.021898403741815287 1106 20 0.2201215661476681 1106 21 0.393155596104215 1106 22 0.5649212742464371 1106 23 -0.9223405149011938 1106 24 0.2425762030411711 1106 25 0.4097539908675394 1106 26 0.5013746232033007 1106 27 0.6307529000098597 1106 28 0.7325537339687678 1106 29 0.7122363529601294 1106 30 -0.3539803976080229 1106 31 0.3924520009857737 1106 32 0.5351577274775465 1106 33 0.6952529103873779 1106 34 0.7591501369987625 1106 35 0.6765287290650157 1106 36 0.7333734239215985 1106 37 0.6526670388993203 1106 38 0.639934352105751 1106 39 0.6637258541244755 1106 40 0.7565175042083823 1106 41 0.8377356838930048 1106 42 0.8802572887016282 1106 43 0.7482495365728283 1106 44 0.6750469255675853 1106 45 0.6703146200658523 1106 46 0.7525243361928737 1106 47 0.8259813249558405 1106 48 0.8702192437304035 1106 49 0.9030548493652653 1106 50 0.8933731767750714 1107 1 0.35548272111821433 1107 2 0.4440461762065375 1107 3 0.3758275186501514 1107 4 0.4186893077782017 1107 5 0.4065698844385963 1107 6 0.4859810322218401 1107 7 0.4903168699483267 1107 8 0.3263633870769098 1107 9 0.6811167009995565 1107 10 0.6657122450878354 1107 11 0.641435799533614 1107 12 0.716153125987617 1107 13 0.5150511983996573 1107 14 0.9813656445770462 1107 15 0.8099526065992748 1107 16 0.6552920476010229 1107 17 0.9288805682803698 1107 18 1.1244984555894888 1107 19 0.7493034890072039 1107 20 0.7548259224380018 1107 21 0.7096811016604647 1107 22 0.888797081782414 1107 23 1.0249435679403582 1107 24 0.8534317298249228 1107 25 0.8675898275092315 1107 26 0.7045428927928121 1107 27 0.7180350228454131 1107 28 0.7352206090568636 1107 29 0.6896359187219154 1107 30 0.9766887311143471 1107 31 0.9859439147034053 1107 32 0.8308175065800256 1107 33 0.6712741839050054 1107 34 0.6579059989677596 1107 35 0.6851171232397348 1107 36 0.7335300660363337 1107 37 0.828907862899257 1107 38 0.9622759705322679 1107 39 0.8308661112783788 1107 40 0.7522883068448145 1107 41 0.7372323237962135 1107 42 0.9107386425142927 1107 43 0.8553045241499859 1107 44 0.7637228511510763 1107 45 0.7311239460427039 1107 46 0.8825994731720463 1107 47 0.9251582808000904 1107 48 0.9173458833140842 1107 49 0.995914325784689 1107 50 1.0880644204016754 1108 1 0.09498035145870887 1108 2 0.2847617967732283 1108 3 0.1585863778929273 1108 4 0.09009930591494353 1108 5 0.1198713855611615 1108 6 0.16275246569519553 1108 7 0.18926122307463444 1108 8 0.292247761713714 1108 9 0.19795747168772948 1108 10 0.2761873115471474 1108 11 0.30922816976500656 1108 12 0.18740857509758868 1108 13 0.2891230740741655 1108 14 0.016618042369913155 1108 15 0.2215375112502258 1108 16 0.42109432815616543 1108 17 0.16828097709044698 1108 18 0.6939911418369817 1108 19 0.35905289912894034 1108 20 0.3534286165997287 1108 21 0.4507874992799259 1108 22 0.37672376346584713 1108 23 0.07076009043506204 1108 24 0.19379240878909698 1108 25 0.37405787261854684 1108 26 0.2943628115842394 1108 27 0.27895985418358293 1108 28 0.49679324571583194 1108 29 0.4657746743232245 1108 30 0.5371428128505329 1108 31 0.6796721574657489 1108 32 0.5015208038104113 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12 -1.7217893915497235 2216 13 -1.7293413677216236 2216 14 -1.7024293583818952 2216 15 -1.765258684872549 2216 16 -1.7724933780060361 2216 17 -1.8003311782022813 2216 18 -1.5037801671083457 2216 19 -1.8156272247466525 2216 20 -1.8029442020794058 2216 21 -2.067127855792762 2216 22 -2.183319990290663 2216 23 -3.697243638057344 2216 24 -2.562243470309955 2216 25 -2.111575461733498 2216 26 -2.2845684048618793 2216 27 -2.2429519150166257 2216 28 -2.7263761256358947 2216 29 -2.3705755798458625 2216 30 -2.149774864889551 2216 31 -1.9061603354287229 2216 32 -1.9550493906620474 2216 33 -1.9506795653507574 2216 34 -2.2413193506749565 2216 35 -2.3087736317995358 2216 36 -2.216624424117004 2216 37 -2.0538278035093747 2216 38 -2.2785176280331036 2216 39 -2.329783195859161 2216 40 -2.3500481385876313 2216 41 -2.3287667135291663 2216 42 -2.7335776111052787 2216 43 -2.491381567517113 2216 44 -2.4916644950372984 2216 45 -2.548764223855901 2216 46 -2.541723548154721 2216 47 -2.6405928845327153 2216 48 -2.739254033281558 2216 49 -2.9620984483055217 2216 50 -3.0076623196138357 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 266693.0 3 12771.0 4 23702.0 5 17694.0 6 13743.0 7 11363.0 8 9978.0 9 8993.0 10 8545.0 11 8331.0 12 8424.0 13 8616.0 14 9181.0 15 9394.0 16 10491.0 17 11227.0 18 12406.0 19 14200.0 20 18298.0 21 24815.0 22 30257.0 23 33481.0 24 34798.0 25 36564.0 26 39608.0 27 44314.0 28 49069.0 29 55187.0 30 63124.0 31 74415.0 32 87891.0 33 107166.0 34 131980.0 35 164201.0 36 209044.0 37 276242.0 38 426202.0 39 644259.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.77297272913023 18.361233544470064 13.147905394468342 23.71788833193136 2 15.155118756422786 20.574804836900242 39.957567326044945 24.312509080632033 3 18.312598722421576 26.3899752086895 29.35358830502931 25.943837763859616 4 12.046597960717182 16.991900261918623 39.0210646270521 31.940437150312096 5 13.181274540215679 38.80508329780687 34.57203190912303 13.441610252854419 6 30.64874575511095 38.11208828971189 18.12374411321208 13.115421841965077 7 27.346634332842928 33.10012695624719 22.179700797157878 17.373537913752003 8 25.93625474222024 35.058067529083516 20.4596578768784 18.54601985181785 9 26.72415737417827 16.28010799408288 19.460162256466045 37.5355723752728 10 14.40756228094443 29.40527677213179 33.92948956527565 22.257671381648127 11 33.76052345383213 24.209739735745764 24.12373787375404 17.90599893666807 12 23.196555123726018 25.776895099921582 30.41710177657532 20.609447999777082 13 27.43057571150836 23.015291471386583 26.29042418002267 23.263708637082388 14 22.0107402150365 21.910065211528632 27.861666242626427 28.217528330808445 15 23.31474531293594 29.71724512466713 24.206857496598722 22.76115206579821 16 22.833042900756322 28.904559973912487 25.8279982311976 22.434398894133594 17 21.907775036594572 28.470446314465175 26.887954910706586 22.733823738233667 18 21.902875480756155 28.19952881418679 27.910759981184412 21.98683572387264 19 22.74300211236957 27.746909730699414 28.227002770494764 21.28308538643625 20 23.278112025107532 27.11951788762323 27.77072789613285 21.831642191136392 21 23.618087618183164 26.96760722201598 27.737242986142974 21.677062173657884 22 23.13649307463051 26.711006812578457 27.694843193923614 22.457656918867418 23 22.938850277606477 26.930631387627677 27.480669387056444 22.6498489477094 24 22.54640475701623 27.01527880003887 27.816252386949714 22.622064055995178 25 22.72594875986778 26.90173290746017 27.767655844233396 22.60466248843866 26 22.50225942288832 27.03367966758282 28.029020147018425 22.435040762510432 27 22.57781558348666 27.220442154279585 27.61500237870748 22.58673988352627 28 22.477357830068005 27.170039834291998 27.795775010078856 22.556827325561137 29 22.685806523322345 27.276983232994525 27.62655713174218 22.410653111940952 30 22.928857800196727 27.227844468809664 27.52376576436106 22.319531966632553 31 23.094711818683344 26.86085205240511 27.45960359456602 22.584832534345527 32 22.670504855406072 27.206795412088304 27.499370494139708 22.62332923836592 33 22.810045174022996 26.961928353873464 27.429260704571444 22.798765767532096 34 22.930307939502736 26.959500429223272 27.697207196090684 22.412984435183304 35 23.14398021094874 27.061647035092655 27.424165414811004 22.370207339147605 36 23.070486471349973 27.042237072127474 27.288851965039278 22.598424491483275 37 23.037384061363653 26.704052829495005 27.651570076160397 22.60699303298094 38 22.928409843750156 26.962461753638195 27.48035307243147 22.628775330180183 39 22.94948480484409 27.2687591999135 27.3062387934357 22.475517201806717 40 23.138944853128972 26.95747874998922 27.629259704221344 22.274316692660463 41 22.95841286635633 27.091587216562544 27.363527966049695 22.586471951031427 42 23.030638946299668 27.18958957126173 27.20401827901671 22.575753203421893 43 22.852815617176745 26.799093600986122 27.40205521632102 22.946035565516116 44 22.866109855009213 27.102651565145703 27.276685606575622 22.75455297326946 45 23.023734290558018 27.098770836483844 27.069686646874825 22.807808226083313 46 23.01557718353167 26.962999712147763 27.085867516339263 22.93555558798131 47 23.034078395131118 27.117709418272167 27.032555708863182 22.81565647773353 48 22.845891696635963 27.298339008817674 27.058860985345447 22.796908309200916 49 23.09087067858743 27.015078283497466 27.045392057592192 22.84865898032291 50 23.113825754058478 27.08677674987464 27.01395097724493 22.785446518821946 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2984.0 1 2402.0 2 1820.0 3 1717.5 4 1615.0 5 1556.0 6 1497.0 7 1452.5 8 1408.0 9 1634.5 10 1861.0 11 2396.0 12 2931.0 13 4884.5 14 6838.0 15 9762.0 16 12686.0 17 13573.0 18 14460.0 19 15170.0 20 15880.0 21 18556.0 22 21232.0 23 26594.0 24 31956.0 25 39590.5 26 47225.0 27 55865.0 28 64505.0 29 73374.5 30 82244.0 31 90561.0 32 98878.0 33 109204.5 34 119531.0 35 129110.0 36 138689.0 37 147106.0 38 155523.0 39 164020.0 40 172517.0 41 179490.0 42 186463.0 43 189978.5 44 193494.0 45 198131.0 46 202768.0 47 206286.0 48 209804.0 49 207221.0 50 204638.0 51 199473.5 52 194309.0 53 186846.0 54 179383.0 55 168939.0 56 158495.0 57 146857.5 58 135220.0 59 122238.0 60 109256.0 61 96288.0 62 83320.0 63 71037.0 64 58754.0 65 49182.0 66 39610.0 67 32315.5 68 25021.0 69 20506.5 70 15992.0 71 12656.5 72 9321.0 73 7776.0 74 6231.0 75 4877.0 76 3523.0 77 2786.5 78 2050.0 79 1596.0 80 1142.0 81 890.5 82 639.0 83 510.0 84 381.0 85 298.5 86 216.0 87 178.0 88 140.0 89 108.0 90 76.0 91 60.5 92 45.0 93 33.0 94 21.0 95 16.0 96 11.0 97 16.5 98 22.0 99 32.0 100 42.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06215468926467521 2 0.066596677724124 3 0.06301656762247872 4 0.07862982556576513 5 0.08227623400262607 6 0.061823197588596945 7 0.04823203886938797 8 0.057314910793932514 9 0.05592264575440378 10 0.06699446773541792 11 0.05356905485424809 12 0.06971269947925972 13 0.06563535186349703 14 0.06682872189737879 15 0.07823203555447121 16 0.07286187040200327 17 0.08482871990842875 18 0.07110496451878845 19 0.06772374942279011 20 0.08061877562223474 21 0.07902761557705906 22 0.07435358294435548 23 0.07120441202161193 24 0.060861871727969974 25 0.07660772634168769 26 0.08695026663532965 27 0.08038673144897995 28 0.09719335942614812 29 0.08990054255242623 30 0.0779668422136086 31 0.07385634543023809 32 0.07949170392356862 33 0.07703866552058945 34 0.0636132526394196 35 0.0820441898293713 36 0.08804418916638794 37 0.09599998939226637 38 0.07717126219102075 39 0.05612154076005075 40 0.06116021423644041 41 0.05542540824028638 42 0.061127065068832594 43 0.04037568614633302 44 0.0386187802631182 45 0.04253038204084176 46 0.04080662532523477 47 0.031292814221788486 48 0.0450497187790366 49 0.05091712144562194 50 0.04146960867739131 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3016667.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.310588112913976 #Duplication Level Percentage of deduplicated Percentage of total 1 79.92866352895462 50.60330695097336 2 10.992276771342864 13.918550141872801 3 3.97254860723209 7.545131658930029 4 1.7860298117505637 4.522983910765009 5 0.9931582808612237 3.143871742526734 6 0.5720264252615588 2.1729197639662274 7 0.3563648194699663 1.5793166412377189 8 0.2588740837960874 1.3111576393857653 9 0.19398107150752983 1.1052950147923444 >10 0.8815812997715996 9.815952077843953 >50 0.047977960130140614 2.076572406005118 >100 0.016043032182308646 1.6933530664397634 >500 2.1080343979504402E-4 0.09829395100306938 >1k 2.635042997028764E-4 0.4132950342580759 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4614 0.1529502593425128 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.3149167607826785E-5 0.0 3 0.0 0.0 0.0 3.3149167607826785E-5 0.0 4 0.0 0.0 0.0 3.3149167607826785E-5 0.0 5 0.0 0.0 0.0 3.3149167607826785E-5 0.0 6 0.0 0.0 0.0 3.3149167607826785E-5 0.0 7 6.629833521565357E-5 0.0 0.0 3.3149167607826785E-5 0.0 8 6.629833521565357E-5 0.0 0.0 3.3149167607826785E-5 0.0 9 6.629833521565357E-5 0.0 0.0 6.629833521565357E-5 0.0 10 6.629833521565357E-5 3.3149167607826785E-5 0.0 6.629833521565357E-5 0.0 11 6.629833521565357E-5 3.3149167607826785E-5 0.0 6.629833521565357E-5 0.0 12 6.629833521565357E-5 3.3149167607826785E-5 0.0 9.944750282348035E-5 3.3149167607826785E-5 13 1.3259667043130714E-4 3.3149167607826785E-5 0.0 9.944750282348035E-5 3.3149167607826785E-5 14 1.3259667043130714E-4 3.3149167607826785E-5 0.0 9.944750282348035E-5 3.3149167607826785E-5 15 1.6574583803913393E-4 6.629833521565357E-5 0.0 9.944750282348035E-5 9.944750282348035E-5 16 1.6574583803913393E-4 6.629833521565357E-5 0.0 9.944750282348035E-5 9.944750282348035E-5 17 1.6574583803913393E-4 6.629833521565357E-5 0.0 9.944750282348035E-5 9.944750282348035E-5 18 1.6574583803913393E-4 9.944750282348035E-5 0.0 1.3259667043130714E-4 9.944750282348035E-5 19 2.9834250847044104E-4 9.944750282348035E-5 0.0 1.3259667043130714E-4 9.944750282348035E-5 20 2.9834250847044104E-4 9.944750282348035E-5 0.0 1.3259667043130714E-4 1.3259667043130714E-4 21 2.9834250847044104E-4 9.944750282348035E-5 0.0 1.3259667043130714E-4 1.3259667043130714E-4 22 2.9834250847044104E-4 1.3259667043130714E-4 0.0 1.6574583803913393E-4 1.3259667043130714E-4 23 2.9834250847044104E-4 1.3259667043130714E-4 0.0 2.3204417325478749E-4 1.3259667043130714E-4 24 3.3149167607826786E-4 2.3204417325478749E-4 0.0 2.651933408626143E-4 1.3259667043130714E-4 25 3.3149167607826786E-4 2.3204417325478749E-4 0.0 3.646408436860946E-4 1.3259667043130714E-4 26 3.3149167607826786E-4 2.651933408626143E-4 0.0 4.309391789017482E-4 1.6574583803913393E-4 27 3.3149167607826786E-4 2.651933408626143E-4 0.0 4.972375141174017E-4 1.6574583803913393E-4 28 3.977900112939214E-4 2.651933408626143E-4 0.0 8.618783578034964E-4 1.6574583803913393E-4 29 4.972375141174017E-4 2.651933408626143E-4 0.0 0.0014254142071365517 1.6574583803913393E-4 30 5.303866817252286E-4 2.651933408626143E-4 0.0 0.0023535909001557017 1.6574583803913393E-4 31 5.303866817252286E-4 2.9834250847044104E-4 0.0 0.004839778470742711 1.6574583803913393E-4 32 5.303866817252286E-4 2.9834250847044104E-4 0.0 0.007856352723054948 1.6574583803913393E-4 33 5.635358493330554E-4 2.9834250847044104E-4 3.3149167607826785E-5 0.010872926975367184 1.6574583803913393E-4 34 5.635358493330554E-4 2.9834250847044104E-4 3.3149167607826785E-5 0.014187843736149864 1.6574583803913393E-4 35 6.629833521565357E-4 2.9834250847044104E-4 3.3149167607826785E-5 0.017867401340618635 1.6574583803913393E-4 36 6.629833521565357E-4 3.646408436860946E-4 3.3149167607826785E-5 0.02307182065504744 1.6574583803913393E-4 37 6.629833521565357E-4 3.646408436860946E-4 3.3149167607826785E-5 0.029569057506181492 1.6574583803913393E-4 38 7.292816873721893E-4 3.646408436860946E-4 3.3149167607826785E-5 0.03729281355880513 1.988950056469607E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6850 0.0 22.483534 1 GGTATCA 3275 0.0 18.81065 1 TATCAAC 8680 0.0 17.51434 2 ATCAACG 8725 0.0 17.272715 3 TCAACGC 8725 0.0 17.096487 4 CAACGCA 8855 0.0 16.79469 5 AACGCAG 8980 0.0 16.535864 6 GTACATG 9630 0.0 15.627406 1 TACATGG 9625 0.0 15.0633 2 AGAGTAC 9275 0.0 14.493148 11 ACGCAGA 10205 0.0 14.33558 7 ACATGGG 10195 0.0 14.005317 3 CTATGCG 110 0.0010186482 13.999947 9 GTATAGA 695 0.0 13.929161 1 CGCAGAG 10350 0.0 13.92219 8 CGTGCCG 175 1.0539534E-6 13.828749 10 GAGTACT 5040 0.0 13.750404 12 ATCGTCG 130 2.3363324E-4 13.535715 44 AGTACTT 5265 0.0 13.455287 13 GGACCGT 215 5.4786142E-8 13.301614 6 >>END_MODULE