FastQCFastQC Report
Mon 6 Feb 2017
SRR2077418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3016667
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51750.1715469423705036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78250.022.2992671
GGTATCA38600.020.0658281
TATACCG1352.5956979E-919.559445
TCGATCG953.3555966E-416.20595241
TAGCGCG1401.5850383E-615.7171464
TATCAAC109950.015.6094182
GCGTAAC850.002980012715.53043611
TCAACGC111500.015.3337334
ATCAACG111550.015.2871553
CAACGCA112000.015.1672985
GTACATG93700.014.8885151
AACGCAG115000.014.7306756
CGTATAC900.00435622314.6690943
ATACCGT1503.2536664E-614.66690356
TACATGG95550.014.438572
GCACCGT3250.014.2156156
ACATGGG99000.013.7578333
ACCGTCC1952.4090878E-713.5386818
CGTCCGA1851.9680392E-613.081943511
GTATTAT6850.012.8490541
Produced by FastQC (version 0.11.5)