##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077418_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3016667
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.610503910441558	31.0	31.0	34.0	27.0	34.0
2	30.559756844225763	31.0	31.0	34.0	27.0	34.0
3	30.724496605027998	31.0	31.0	34.0	27.0	34.0
4	34.20293920409512	37.0	35.0	37.0	30.0	37.0
5	33.839927641996944	37.0	35.0	37.0	28.0	37.0
6	33.78659063131595	37.0	35.0	37.0	29.0	37.0
7	33.6079424742605	37.0	35.0	37.0	28.0	37.0
8	33.576606234629146	37.0	35.0	37.0	28.0	37.0
9	35.02080077118224	39.0	35.0	39.0	28.0	39.0
10	34.88760509529225	39.0	35.0	39.0	27.0	39.0
11	34.822144108050374	39.0	35.0	39.0	27.0	39.0
12	34.75189406056419	39.0	35.0	39.0	27.0	39.0
13	34.72183273791904	39.0	35.0	39.0	27.0	39.0
14	35.873951616137944	40.0	36.0	41.0	27.0	41.0
15	35.88350951563431	40.0	36.0	41.0	27.0	41.0
16	35.83618410649899	40.0	36.0	41.0	27.0	41.0
17	35.824988638122804	40.0	36.0	41.0	27.0	41.0
18	35.83211902407525	40.0	36.0	41.0	27.0	41.0
19	35.879885317139745	40.0	36.0	41.0	27.0	41.0
20	35.88527901820121	40.0	36.0	41.0	27.0	41.0
21	35.824352837088085	40.0	36.0	41.0	27.0	41.0
22	35.82404256087928	40.0	36.0	41.0	27.0	41.0
23	35.761086656233516	40.0	36.0	41.0	27.0	41.0
24	35.74237627156063	40.0	36.0	41.0	27.0	41.0
25	35.722066108058996	40.0	36.0	41.0	27.0	41.0
26	35.644752635938936	40.0	36.0	41.0	27.0	41.0
27	35.58107905181447	40.0	35.0	41.0	26.0	41.0
28	35.51906590949548	40.0	35.0	41.0	26.0	41.0
29	35.43942801774276	40.0	35.0	41.0	25.0	41.0
30	35.30487620940595	39.0	35.0	41.0	25.0	41.0
31	35.212601523469445	39.0	35.0	41.0	25.0	41.0
32	35.12351313552341	39.0	35.0	41.0	25.0	41.0
33	35.02148231806825	39.0	35.0	41.0	24.0	41.0
34	34.98767812290849	39.0	35.0	41.0	24.0	41.0
35	34.91450199839757	39.0	35.0	41.0	24.0	41.0
36	34.80365648578381	39.0	34.0	41.0	24.0	41.0
37	34.70779970079561	39.0	34.0	41.0	23.0	41.0
38	34.62928457134977	39.0	34.0	41.0	23.0	41.0
39	34.534154416115534	39.0	34.0	41.0	22.0	41.0
40	34.4385336532007	39.0	34.0	41.0	21.0	41.0
41	34.37672470975418	39.0	34.0	41.0	20.0	41.0
42	34.25730615941369	39.0	34.0	41.0	19.0	41.0
43	34.339995100553026	39.0	34.0	41.0	19.0	41.0
44	34.265866268965055	39.0	34.0	41.0	17.0	41.0
45	34.18189909592275	39.0	34.0	41.0	15.0	41.0
46	34.017607180374895	39.0	34.0	41.0	10.0	41.0
47	33.85781294388807	39.0	33.0	41.0	7.0	41.0
48	33.69035461985032	39.0	33.0	41.0	2.0	41.0
49	33.53947585199162	39.0	33.0	41.0	2.0	41.0
50	33.39238802294055	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6646501345674274
1101	2	0.8038859629371657
1101	3	0.6716682507566034
1101	4	0.776356324224146
1101	5	0.7043180494308316
1101	6	0.821630618751719
1101	7	0.7915249237773523
1101	8	0.7713390032334928
1101	9	0.8392137436728433
1101	10	0.9484571845883849
1101	11	0.9894446460426067
1101	12	0.9359485813511839
1101	13	0.9794593173585469
1101	14	1.077234629401211
1101	15	0.948113596158052
1101	16	1.0567690273289045
1101	17	1.0187889287794931
1101	18	0.9785603915240628
1101	19	0.9586573651507564
1101	20	0.8950856604359245
1101	21	1.00951390215819
1101	22	0.9614399211845424
1101	23	0.9929024278930214
1101	24	0.9701249842589945
1101	25	0.9790742606638787
1101	26	0.9585766893619123
1101	27	1.0272935032933859
1101	28	1.0115782820250985
1101	29	1.0106837591813118
1101	30	1.1350418308834662
1101	31	1.1798317823511084
1101	32	1.1609744636185368
1101	33	1.166343117460265
1101	34	1.1225557271315623
1101	35	1.0955531980227775
1101	36	1.07931708889857
1101	37	1.139845887681389
1101	38	1.1569083151571604
1101	39	1.0784339415839739
1101	40	1.2064485821762503
1101	41	1.2329321739222365
1101	42	1.214827726856413
1101	43	1.1539422024904127
1101	44	1.2780337758488258
1101	45	1.225334536485562
1101	46	1.2108483799115675
1101	47	1.26698671296095
1101	48	1.309483477634572
1101	49	1.3549180194628079
1101	50	1.3845808338206567
1102	1	0.6382933796273491
1102	2	0.798137064763484
1102	3	0.7069567879437173
1102	4	0.6592319445186305
1102	5	0.6680843985983813
1102	6	0.6374367278726254
1102	7	0.6521859941083719
1102	8	0.7185479390385865
1102	9	0.7279959677961543
1102	10	0.8151843975979531
1102	11	0.8678021037072696
1102	12	0.7294713447901557
1102	13	0.7747214259906059
1102	14	0.8580363974155318
1102	15	0.8962870978397532
1102	16	0.931762878488783
1102	17	0.8638489022490958
1102	18	0.8474306599088379
1102	19	0.8731687296840462
1102	20	0.8961674998429885
1102	21	0.9225445546990727
1102	22	0.8829636623272847
1102	23	0.9370011963427274
1102	24	0.860269933935264
1102	25	0.8570763331921825
1102	26	0.836122143675901
1102	27	0.8585769686421898
1102	28	0.8848223998893303
1102	29	0.9938818180319515
1102	30	1.0036708304939168
1102	31	0.9721042561707947
1102	32	0.982454615468157
1102	33	1.0033292437419092
1102	34	1.0844426954096562
1102	35	1.000797611235015
1102	36	0.9895366903315193
1102	37	0.9748406045164799
1102	38	1.0295205410372859
1102	39	0.9486597555096381
1102	40	1.0670119839743109
1102	41	0.9684815339590997
1102	42	1.0417937643808912
1102	43	0.9965387189115091
1102	44	0.9819871617846587
1102	45	0.8441262575751907
1102	46	1.0866718534588244
1102	47	1.1119556886889441
1102	48	1.0894571000015816
1102	49	1.1271191514604553
1102	50	1.1344210658216127
1103	1	0.4584212718267331
1103	2	0.6093650517725422
1103	3	0.4781330330771567
1103	4	0.4744994288346902
1103	5	0.5340932138808938
1103	6	0.6025242755296176
1103	7	0.587193659571426
1103	8	0.5440016134177839
1103	9	0.5952516311612968
1103	10	0.7006786182422573
1103	11	0.6121501359022119
1103	12	0.7330735072576289
1103	13	0.8016511054532103
1103	14	0.7986938117338056
1103	15	0.8492368890567477
1103	16	0.915684267147931
1103	17	0.8923103631249774
1103	18	0.8909307506840634
1103	19	0.8461079304586576
1103	20	0.8423660353360987
1103	21	0.883606624777542
1103	22	0.8820059837522507
1103	23	0.9246209692029481
1103	24	0.8895123642904963
1103	25	0.861684385962846
1103	26	0.7797975314878158
1103	27	0.7924452033667322
1103	28	0.8418482002241916
1103	29	0.9116065875637531
1103	30	0.9511789498330714
1103	31	0.9013935870565604
1103	32	0.8395871271176389
1103	33	0.8446165372282834
1103	34	0.8989584600395943
1103	35	0.9596084558480129
1103	36	0.9806194369879648
1103	37	0.9245530487905782
1103	38	0.8998261509459056
1103	39	0.767809393417366
1103	40	0.8827720751529782
1103	41	0.9554403623535919
1103	42	0.8916858427299914
1103	43	0.9321206889355125
1103	44	0.9134205025144908
1103	45	0.8366742171499624
1103	46	0.880063417415009
1103	47	0.8985664445439454
1103	48	0.9039322175050657
1103	49	0.8829317914055892
1103	50	0.8358941459257068
1104	1	0.4604703737067979
1104	2	0.6341947734585673
1104	3	0.5804371676295865
1104	4	0.522438477131054
1104	5	0.508144221599359
1104	6	0.43774379622448123
1104	7	0.5812920823707586
1104	8	0.5703078763998022
1104	9	0.5832620467714094
1104	10	0.5439329310452408
1104	11	0.568603316963177
1104	12	0.6051243219847962
1104	13	0.6726061814992832
1104	14	0.7854700598330027
1104	15	0.6962828000684169
1104	16	0.6695813396396488
1104	17	0.717465180428519
1104	18	0.7101039230781723
1104	19	0.7173942292999627
1104	20	0.7635671752871573
1104	21	0.747699015118819
1104	22	0.8092304551277749
1104	23	0.8066991914570991
1104	24	0.7795973574213448
1104	25	0.715243506617135
1104	26	0.8057394691283406
1104	27	0.8576378809059051
1104	28	0.8181846649630202
1104	29	0.8463320169891233
1104	30	0.8587130301624413
1104	31	0.8984768136658374
1104	32	0.9171239464237146
1104	33	0.919642052335476
1104	34	0.869770696871889
1104	35	0.903767173628033
1104	36	0.9684041040858631
1104	37	0.9745455402845593
1104	38	0.9724419881512318
1104	39	0.9200068598244471
1104	40	0.9205940775817467
1104	41	0.9577453059697447
1104	42	0.9450615525847397
1104	43	0.9075584516098871
1104	44	1.0043448104591448
1104	45	0.8926195923246496
1104	46	0.959022116882565
1104	47	0.9917686615364119
1104	48	0.9690940618230499
1104	49	1.0089324157659831
1104	50	0.9712218443604215
1105	1	0.42575934244941394
1105	2	0.5334641657297965
1105	3	0.39032236443766877
1105	4	0.5089509964910661
1105	5	0.5798700602221416
1105	6	0.5496733022334155
1105	7	0.5313084493070903
1105	8	0.549877477902406
1105	9	0.5309972358960735
1105	10	0.5802395943500471
1105	11	0.5624437426035342
1105	12	0.48976045191019324
1105	13	0.583333102340049
1105	14	0.575427453870553
1105	15	0.6546714992277813
1105	16	0.6792531219836278
1105	17	0.7411659370579429
1105	18	0.623970516584059
1105	19	0.6463126344487549
1105	20	0.709954400075965
1105	21	0.6868379084792835
1105	22	0.7340636652763521
1105	23	0.7590233733458831
1105	24	0.6235799696190014
1105	25	0.6207495959444529
1105	26	0.6096034219992603
1105	27	0.6567927815532144
1105	28	0.7593430286309584
1105	29	0.757827279905392
1105	30	0.8097270397598919
1105	31	0.8117593133335959
1105	32	0.7767336171098052
1105	33	0.7547397837165448
1105	34	0.6764095282072446
1105	35	0.7333820775025757
1105	36	0.8407431546920066
1105	37	0.7983341706297011
1105	38	0.7848420387714157
1105	39	0.8090401854839797
1105	40	0.8744372496363297
1105	41	0.8503859447841506
1105	42	0.9234112307369173
1105	43	0.8671386579640696
1105	44	0.8620895928010057
1105	45	0.7856847425398499
1105	46	0.804484725164464
1105	47	0.8468192942213975
1105	48	0.7770021961904021
1105	49	0.8357053675092985
1105	50	0.9017443919788803
1106	1	0.2927366009567507
1106	2	0.4568578049690011
1106	3	0.3850062804587395
1106	4	0.36326990526794845
1106	5	0.35453568925415624
1106	6	0.27386100762220167
1106	7	0.31160639922347144
1106	8	0.2607278210052897
1106	9	0.3608684831079927
1106	10	0.24721768356926077
1106	11	0.24493952957276122
1106	12	0.29260713157372464
1106	13	0.29283939139530446
1106	14	0.35763273712252897
1106	15	0.4379841780432301
1106	16	0.3535396820850636
1106	17	0.4976305745975864
1106	18	0.34987269799567144
1106	19	0.28673305395069804
1106	20	0.39199414510783726
1106	21	0.40480018358764625
1106	22	0.4052192912158574
1106	23	0.5227227560892018
1106	24	0.3975302424512108
1106	25	0.4465656476388986
1106	26	0.4385255808985775
1106	27	0.4608033074278097
1106	28	0.3508446196329942
1106	29	0.4510897535161362
1106	30	0.4763976711877618
1106	31	0.38916797184342755
1106	32	0.46973611566455276
1106	33	0.49127403868693875
1106	34	0.4364328221233009
1106	35	0.4857223250018521
1106	36	0.45453123480737645
1106	37	0.5356603394507431
1106	38	0.4761847183684935
1106	39	0.5576392087933755
1106	40	0.6159604013873761
1106	41	0.5604643866405539
1106	42	0.6480343990644428
1106	43	0.5952079589691195
1106	44	0.588587715580978
1106	45	0.5857476878438916
1106	46	0.5439771639872575
1106	47	0.5558955954843441
1106	48	0.5354866907645359
1106	49	0.5110857297072258
1106	50	0.5260404108677577
1107	1	0.3270353552443801
1107	2	0.5099594818640618
1107	3	0.4400716430258491
1107	4	0.492465973965416
1107	5	0.5199138972851358
1107	6	0.48712773304557544
1107	7	0.4825382060127126
1107	8	0.5112865484769813
1107	9	0.5003859303753089
1107	10	0.6115902985964112
1107	11	0.571310059578316
1107	12	0.6023534291046886
1107	13	0.6563227375391634
1107	14	0.6471293965885323
1107	15	0.5565504746407655
1107	16	0.6352569622115638
1107	17	0.626344094288612
1107	18	0.7384322435649295
1107	19	0.7247165170521441
1107	20	0.693588298849626
1107	21	0.7508466959088125
1107	22	0.6198174979035258
1107	23	0.7271864089236928
1107	24	0.6535529524371171
1107	25	0.6419395397441079
1107	26	0.7603687671901653
1107	27	0.7316967711338407
1107	28	0.6204067883870863
1107	29	0.6474837989359159
1107	30	0.6993180316909928
1107	31	0.7171624003586814
1107	32	0.7170035887594182
1107	33	0.7486721064973736
1107	34	0.6735228895227223
1107	35	0.6142369096729468
1107	36	0.6952508998252043
1107	37	0.7740028916747974
1107	38	0.7052155186890587
1107	39	0.6278069579193115
1107	40	0.5777658091221838
1107	41	0.5714602625233596
1107	42	0.5172162949808552
1107	43	0.5563432477313768
1107	44	0.5965441382383858
1107	45	0.573396296402656
1107	46	0.6805118435640054
1107	47	0.7040546756825918
1107	48	0.7967060250313551
1107	49	0.8113376426460661
1107	50	0.6661075261683678
1108	1	0.295368670129605
1108	2	0.4857203740883662
1108	3	0.28384817371150817
1108	4	0.26861012563838216
1108	5	0.3045201039666736
1108	6	0.3680964801054145
1108	7	0.3249301600547838
1108	8	0.2971434446565624
1108	9	0.29726178936535064
1108	10	0.356541835416067
1108	11	0.2630090565546723
1108	12	0.27839528601747077
1108	13	0.34073602312879814
1108	14	0.33285729437125866
1108	15	0.3609303322582704
1108	16	0.3922099158459318
1108	17	0.3121246153000854
1108	18	0.3165322636733663
1108	19	0.4033655989820346
1108	20	0.2359807140944099
1108	21	0.3995137278480172
1108	22	0.2497191602625577
1108	23	0.38214908840073036
1108	24	0.275084108524716
1108	25	0.2722722421521411
1108	26	0.3309238338708482
1108	27	0.28339450441477965
1108	28	0.22294926054427577
1108	29	0.29828928946189137
1108	30	0.37397429731423415
1108	31	0.3773054469899648
1108	32	0.33551617313645465
1108	33	0.25106860799812836
1108	34	0.303278765706402
1108	35	0.377437868605611
1108	36	0.4212633781256159
1108	37	0.40017144097090807
1108	38	0.3134526451379287
1108	39	0.4157566536177697
1108	40	0.41894226343268315
1108	41	0.4798192848474159
1108	42	0.4263875413903975
1108	43	0.3564834994742654
1108	44	0.3433618856874716
1108	45	0.4388040641631079
1108	46	0.3820118131246275
1108	47	0.38551806264457156
1108	48	0.3954135966884138
1108	49	0.33713547720712
1108	50	0.4322747103044762
1109	1	0.22674476773246255
1109	2	0.4072952686436011
1109	3	0.27551662138551336
1109	4	0.23793492292321616
1109	5	0.32036691358885605
1109	6	0.4044630059354972
1109	7	0.3817671732232739
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2210	49	-0.28063141220839327
2210	50	-0.25586231226218814
2211	1	-0.1714007618085418
2211	2	-0.19474634669807855
2211	3	-0.17376829507971436
2211	4	-0.15858377271256785
2211	5	-0.20845925218598182
2211	6	-0.21474790594663773
2211	7	-0.27594185378848834
2211	8	-0.2327201254330049
2211	9	-0.3461058227952236
2211	10	-0.37163262788128293
2211	11	-0.4702175504378161
2211	12	-0.3033348823769373
2211	13	-0.2949679879657623
2211	14	-0.5035940681475921
2211	15	-0.5605677708790751
2211	16	-0.4926920454619861
2211	17	-0.43489723912126976
2211	18	-0.3861811578538479
2211	19	-0.37325928507880235
2211	20	-0.2823891678924326
2211	21	-0.3267126459309466
2211	22	-0.4208571708792803
2211	23	-0.37600194672906184
2211	24	-0.35431040864317964
2211	25	-0.2557733306184886
2211	26	-0.3651847937832855
2211	27	-0.3228360796693792
2211	28	-0.321768408673492
2211	29	-0.2629767484183887
2211	30	-0.28322220911159945
2211	31	-0.35232281777681607
2211	32	-0.2866821046439938
2211	33	-0.26871688896920887
2211	34	-0.28158282829912196
2211	35	-0.27935741264811753
2211	36	-0.33831457660959785
2211	37	-0.33046222331199715
2211	38	-0.3633590322725837
2211	39	-0.39686180015877426
2211	40	-0.3949589537282776
2211	41	-0.3551011466154037
2211	42	-0.4095773216990253
2211	43	-0.5182986862171077
2211	44	-0.4980550110772697
2211	45	-0.572259514115629
2211	46	-0.5379606552179723
2211	47	-0.41084260473775913
2211	48	-0.5657989445022551
2211	49	-0.5828963181631437
2211	50	-0.5852838645650777
2212	1	-0.8329460445058636
2212	2	-2.2171563613355367
2212	3	-1.1699968806052432
2212	4	-0.6742432997622103
2212	5	-0.5884139535900275
2212	6	-0.42831355639896174
2212	7	-0.4325642323452712
2212	8	-0.5439800544858073
2212	9	-0.6148026137367921
2212	10	-0.49338046450623096
2212	11	-0.5226933961289149
2212	12	-0.47961368614245004
2212	13	-2.001017872870385
2212	14	-1.3767456289109106
2212	15	-1.010242572405204
2212	16	-0.7159038781169187
2212	17	-0.7058197045310237
2212	18	-0.7019907751326926
2212	19	-0.560033999323224
2212	20	-0.6317132795429856
2212	21	-0.6265044363776937
2212	22	-0.599498707823237
2212	23	-0.5472607575185506
2212	24	-0.603026460151888
2212	25	-0.6947330795701632
2212	26	-0.7606563364829029
2212	27	-0.7296946600894785
2212	28	-0.7661034582254231
2212	29	-0.6674369130567612
2212	30	-0.6646188181656711
2212	31	-0.6236848624909115
2212	32	-0.5357754807023696
2212	33	-0.5744972831338018
2212	34	-0.6180587018574784
2212	35	-0.6659162091824271
2212	36	-0.5786219043068357
2212	37	-0.6275539259054028
2212	38	-0.771883664254986
2212	39	-0.7403057849918397
2212	40	-0.5715039255984138
2212	41	-0.6645177722254942
2212	42	-0.6063806338306108
2212	43	-0.7080068319972028
2212	44	-0.6566659690573644
2212	45	-0.8562077374524648
2212	46	-0.7338862824904524
2212	47	-0.7849342837511699
2212	48	-0.7093407684433046
2212	49	-0.7464214271045009
2212	50	-0.7659682417120663
2213	1	-0.8199114668382279
2213	2	-2.122832700947356
2213	3	-1.1210414161938544
2213	4	-0.705165791749522
2213	5	-0.6813982276141246
2213	6	-0.6304152079961796
2213	7	-0.5887457276747341
2213	8	-0.586899667856585
2213	9	-0.6653153898522106
2213	10	-0.778594593174617
2213	11	-0.617738604476898
2213	12	-0.6597811274744032
2213	13	-0.7707188556762432
2213	14	-0.7214000325990071
2213	15	-0.7265659604430681
2213	16	-0.9357842333627318
2213	17	-0.8510335987256354
2213	18	-0.835355762426019
2213	19	-0.8229333036575639
2213	20	-0.9805760817210611
2213	21	-0.8858943446263012
2213	22	-0.9292186649686442
2213	23	-1.000913949157578
2213	24	-0.8675673514769002
2213	25	-0.9032211655967899
2213	26	-0.8899785900284201
2213	27	-0.8342502150438236
2213	28	-0.8688535650913565
2213	29	-0.9531430759294608
2213	30	-1.0263559892936556
2213	31	-0.9026050385987574
2213	32	-0.8470703080304673
2213	33	-0.8897049367186369
2213	34	-0.9507453384754285
2213	35	-0.8802874677474861
2213	36	-0.9315379915527231
2213	37	-0.8309361650578353
2213	38	-0.8779821468489146
2213	39	-0.9045162035636025
2213	40	-0.8810698695447527
2213	41	-0.814133099956706
2213	42	-0.8840973732743294
2213	43	-0.9855943403165099
2213	44	-0.937661463553539
2213	45	-0.9555554494393022
2213	46	-0.9118347451541098
2213	47	-0.9700651291373532
2213	48	-1.0459497455424298
2213	49	-0.9562372208974352
2213	50	-0.9588479945406263
2214	1	-0.7440179294778453
2214	2	-1.6674607788363467
2214	3	-0.8100636258837746
2214	4	-0.5004207452189462
2214	5	-0.37630061103344303
2214	6	-0.3301284545440595
2214	7	-0.2985191402785432
2214	8	-0.2885536455674256
2214	9	-0.4605591170791712
2214	10	-0.5128368559272474
2214	11	-0.5157213807794889
2214	12	-0.4569861893210927
2214	13	-0.47723031844022756
2214	14	-0.5456616595192685
2214	15	-0.608995988484466
2214	16	-0.5882754136084145
2214	17	-0.5501300182806901
2214	18	-0.6423989308502485
2214	19	-0.6392384743107513
2214	20	-0.7490686857184414
2214	21	-0.7259072261892214
2214	22	-0.6572444509872213
2214	23	-0.6070772883439659
2214	24	-0.6095683125313087
2214	25	-0.565348864324946
2214	26	-0.6247413101865931
2214	27	-0.5113689096651513
2214	28	-0.6847548111382338
2214	29	-0.6298787575725981
2214	30	-0.5623241877700949
2214	31	-0.600478414067922
2214	32	-0.6860936953752343
2214	33	-0.7082466830420984
2214	34	-0.6891008524425288
2214	35	-0.724734891181761
2214	36	-0.7850232445625096
2214	37	-0.6687502439545412
2214	38	-0.86780874982977
2214	39	-0.9670555752066008
2214	40	-0.8174406718931095
2214	41	-0.7219255430225928
2214	42	-0.7579778472506931
2214	43	-0.6430985881285451
2214	44	-0.7108222033144926
2214	45	-0.7475092712814799
2214	46	-0.6207327749925824
2214	47	-0.5990563940201099
2214	48	-0.6327720739663789
2214	49	-0.5482814690220508
2214	50	-0.6435482769796792
2215	1	-0.5449730730211257
2215	2	-0.27328348098290434
2215	3	-0.5666868717673559
2215	4	-0.5307680554813743
2215	5	-0.6992430492346884
2215	6	-0.722659580313568
2215	7	-0.8724769272399442
2215	8	-0.8402963627270665
2215	9	-0.9259526678378691
2215	10	-0.9655459394806982
2215	11	-1.0016762269508348
2215	12	-0.9776795380348844
2215	13	-0.8799976751329908
2215	14	-0.96144995892476
2215	15	-1.0602458074571999
2215	16	-1.1038550990393077
2215	17	-1.1439039067396664
2215	18	-1.1585003192243875
2215	19	-1.1679388144572087
2215	20	-1.0393862722276381
2215	21	-1.0566207743538953
2215	22	-1.1400700455378825
2215	23	-1.0926536993234066
2215	24	-1.1505486856474008
2215	25	-1.0762141965028391
2215	26	-1.1206274550399868
2215	27	-1.198164604391522
2215	28	-1.2947281619084237
2215	29	-1.2781968336534462
2215	30	-1.0319310956698118
2215	31	-1.0836180552256707
2215	32	-1.016132864467643
2215	33	-1.0636691511216583
2215	34	-1.1214321039482869
2215	35	-1.3254383438211619
2215	36	-1.1662498265224173
2215	37	-1.1664595559971644
2215	38	-1.1891231186737912
2215	39	-1.136083422660512
2215	40	-1.0807809534253678
2215	41	-1.165130023023238
2215	42	-1.088503185859885
2215	43	-1.0562622441703553
2215	44	-1.0223146038654178
2215	45	-1.0504082528581975
2215	46	-0.9365223680500065
2215	47	-1.0027634124346534
2215	48	-1.0141143285698462
2215	49	-0.9466769962120907
2215	50	-0.8659802182875325
2216	1	-1.0967054820458557
2216	2	-0.787684829501579
2216	3	-0.8500215124227779
2216	4	-2.2928990478791356
2216	5	-1.5882325115809408
2216	6	-1.4031146686981089
2216	7	-1.242589934074367
2216	8	-1.1449999800553954
2216	9	-1.3685183416412414
2216	10	-1.280062842517573
2216	11	-1.4469290056479878
2216	12	-1.3468125315920148
2216	13	-1.482555339197134
2216	14	-1.5683126983958786
2216	15	-1.6530092376407737
2216	16	-1.6157617740983454
2216	17	-1.5547918909191623
2216	18	-1.6391121746587984
2216	19	-1.6814739026631855
2216	20	-1.646401904611217
2216	21	-1.5944840106074096
2216	22	-1.7285504015886488
2216	23	-1.6795103453981568
2216	24	-1.7713784079020414
2216	25	-1.8788012274850985
2216	26	-1.924365328033332
2216	27	-1.9185703397729483
2216	28	-1.9142747953627364
2216	29	-1.8859076584716448
2216	30	-1.7971446578929218
2216	31	-1.7825195379840082
2216	32	-1.7583292676100584
2216	33	-1.7723531496906162
2216	34	-1.806577290037616
2216	35	-1.81646844028878
2216	36	-1.9985246275384299
2216	37	-1.9141644085854423
2216	38	-1.9794925073125853
2216	39	-2.1501225473031482
2216	40	-2.1303212065101675
2216	41	-2.160544192226432
2216	42	-2.0952889049284806
2216	43	-1.9399364856525168
2216	44	-1.9266769782429307
2216	45	-1.8132196107449516
2216	46	-1.818372496645857
2216	47	-1.795198449686957
2216	48	-1.798575737378389
2216	49	-1.7383868682794663
2216	50	-1.8262396994018317
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68116.0
3	11160.0
4	19624.0
5	11856.0
6	8891.0
7	6797.0
8	5931.0
9	5194.0
10	4997.0
11	4874.0
12	5118.0
13	5413.0
14	5805.0
15	6282.0
16	7032.0
17	7508.0
18	8127.0
19	9001.0
20	10744.0
21	13384.0
22	17129.0
23	21344.0
24	25046.0
25	28041.0
26	30384.0
27	34311.0
28	40306.0
29	46796.0
30	53223.0
31	62924.0
32	77050.0
33	96164.0
34	120467.0
35	153428.0
36	197356.0
37	264307.0
38	416231.0
39	1106306.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.217176119309954	18.989255062062966	14.41954823452338	25.374020584103697
2	14.125276092777117	20.72279539316639	39.24662763218392	25.905300881872567
3	17.182127736311646	25.80158400770125	30.192610881202075	26.823677374785028
4	11.48332670885778	17.066914275087342	38.37301775414698	33.07674126190789
5	12.8060308620051	38.71048937412761	34.82997244499362	13.65350731887367
6	30.88897878520601	38.207286907043525	17.791446439111088	13.112287868639374
7	27.01783554767536	32.864003575929985	22.344124237039324	17.774036639355327
8	26.27190466559015	34.62573485283048	20.337709016515745	18.76465146506361
9	26.54085326607604	15.258135553372155	19.668214464186057	38.532796716365745
10	14.015972739433579	29.205760107811347	34.350807358177605	22.427459794577466
11	34.20115957043563	23.385775878507424	24.1567777963121	18.25628675474485
12	23.158355875820824	25.278991263894845	30.9269663200811	20.63568654020323
13	27.70061349693251	22.220195655778475	26.719714806831373	23.359476040457636
14	21.806494875686948	21.097816458624276	28.556632754414025	28.539055911274748
15	23.223528522234663	29.296077032053358	24.620092642213226	22.860301803498757
16	22.651760918143523	28.33397782378017	26.323764584774594	22.690496673301713
17	21.60562183545406	27.903754357443166	27.55035000495814	22.940273802144638
18	21.528886766264197	27.458273012651173	28.90553822631491	22.10730199476972
19	22.52260708203224	27.135297561105038	29.081770226145444	21.260325130717277
20	22.929996298767716	26.38753095968045	28.80149057512374	21.880982166428097
21	23.16547740851722	26.269333390820133	28.725401592273776	21.83978760838887
22	22.58656027380905	26.18096735251589	28.73970231225375	22.492770061421314
23	22.426393285934783	26.251541315207	28.55646669200426	22.765598706853954
24	22.233077807017107	26.412533225882946	28.811495865315578	22.542893101784372
25	22.325053409060008	26.38271595931029	28.766076607926095	22.526154023703608
26	22.237282402141073	26.4959158428155	28.903761138987367	22.36304061605606
27	22.20371669294916	26.721851614176295	28.608520171192726	22.465911521681814
28	22.112346940873625	26.62139368787181	28.823435382700048	22.442823988554515
29	22.38041434759207	26.753379578303445	28.583623152022408	22.282582922082078
30	22.541557826122858	26.666085225752173	28.53973988367423	22.252617064450735
31	22.668133731005927	26.23236389032036	28.545212044518635	22.554290334155073
32	22.30724847214715	26.771894802813307	28.407484903852207	22.51337182118733
33	22.47388688949766	26.518691821196732	28.38329821964656	22.624123069659056
34	22.55072944297082	26.50086206896552	28.672745358090186	22.275663129973474
35	22.799536336140385	26.621679601111147	28.3530914350171	22.22569262773137
36	22.710713680593937	26.59893518713471	28.15533787433325	22.535013257938104
37	22.644569131552412	26.279847073174047	28.57746288812344	22.498120907150103
38	22.65231582116394	26.4612557675041	28.40864631589278	22.47778209543918
39	22.675745156013466	26.778570751279585	28.116738401187714	22.428945691519235
40	22.798147329783188	26.42949234568458	28.443513681693062	22.32884664283917
41	22.594361421466537	26.70699905915395	28.079082784506475	22.61955673487303
42	22.690893395145547	26.72275471017568	28.010593917083938	22.57575797759484
43	22.60821108400537	26.299940230346873	28.13237900789006	22.9594696777577
44	22.595346916016613	26.65904644962087	27.9750919680415	22.770514666321017
45	22.765865472909404	26.637929948531152	27.76029294081845	22.835911637740995
46	22.826661309688372	26.559507211036436	27.596029471336518	23.01780200793867
47	22.814999567400495	26.719102367628693	27.565472526284978	22.90042553868583
48	22.70131669672218	26.936856900392492	27.46091678158481	22.90090962130052
49	22.911192208869345	26.617740607910534	27.498747787703024	22.972319395517097
50	22.9110844516548	26.76665399255929	27.473597359188958	22.848664196596953
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	589.0
1	450.0
2	311.0
3	535.5
4	760.0
5	849.5
6	939.0
7	898.0
8	857.0
9	1164.5
10	1472.0
11	2182.0
12	2892.0
13	5784.5
14	8677.0
15	11988.5
16	15300.0
17	17545.0
18	19790.0
19	20898.5
20	22007.0
21	24098.0
22	26189.0
23	30545.5
24	34902.0
25	41458.0
26	48014.0
27	56195.5
28	64377.0
29	72873.0
30	81369.0
31	89040.5
32	96712.0
33	106879.5
34	117047.0
35	127333.5
36	137620.0
37	145777.5
38	153935.0
39	162325.5
40	170716.0
41	177625.0
42	184534.0
43	188763.5
44	192993.0
45	198198.0
46	203403.0
47	207671.5
48	211940.0
49	208808.0
50	205676.0
51	201143.5
52	196611.0
53	189143.5
54	181676.0
55	170719.0
56	159762.0
57	147216.5
58	134671.0
59	121805.0
60	108939.0
61	94924.0
62	80909.0
63	68608.5
64	56308.0
65	46685.0
66	37062.0
67	30103.5
68	23145.0
69	18744.5
70	14344.0
71	11188.5
72	8033.0
73	6824.0
74	5615.0
75	4245.0
76	2875.0
77	2254.5
78	1634.0
79	1285.0
80	936.0
81	729.0
82	522.0
83	411.0
84	300.0
85	228.0
86	156.0
87	115.0
88	74.0
89	56.5
90	39.0
91	28.0
92	17.0
93	12.5
94	8.0
95	7.0
96	6.0
97	4.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015116020429169014
2	5.966850169408821E-4
3	4.972375141174017E-4
4	0.0025193367381948356
5	0.04123756450413652
6	0.028077344963829284
7	0.03142541089221979
8	0.025093919879124876
9	0.03261878092610156
10	0.03411049346845376
11	0.041104967833705215
12	0.04143645950978348
13	0.03868507859833386
14	0.04428728792405658
15	0.039613255291353004
16	0.04524861378468356
17	0.04720441467354534
18	0.05078452477519064
19	0.0521104914795037
20	0.04823203886938797
21	0.049127066394799294
22	0.04730386217636882
23	0.04591159713684009
24	0.04382319957754701
25	0.041801100353469574
26	0.04498342044382094
27	0.04481767460578181
28	0.0440552437508018
29	0.04229833786758698
30	0.03878452610115733
31	0.03716021688837382
32	0.0330497201050033
33	0.02482872653826226
34	0.022110494794420465
35	0.022839776481792654
36	0.024464085694576164
37	0.019359113882970843
38	0.0209171247605387
39	0.015016572926345532
40	0.008751380248466271
41	0.007723756052623641
42	0.007259667706114066
43	0.0028508284142731034
44	0.004309391789017482
45	0.0028176792466652768
46	0.0013591158719208983
47	6.629833521565357E-4
48	8.950275254113231E-4
49	1.988950056469607E-4
50	5.966850169408821E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3016667.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.951377750797285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.87931993878718	42.64480435556676
2	13.489469259210235	15.364877188780996
3	4.92786802693985	8.419466205249822
4	2.32418042725951	5.294611098954632
5	1.2916711620702777	3.6781226140437844
6	0.7542213330962811	2.577236642932573
7	0.48625595759549683	1.9385062707217762
8	0.3370462740975141	1.5356199740501029
9	0.24087118805362268	1.2346151418112676
>10	1.1804551215054262	11.90152426350511
>50	0.06365648511268782	2.4765711075281795
>100	0.02422422006175698	2.314104045092614
>500	5.265735302117921E-4	0.19602867208381639
>1k	1.755245099597854E-4	0.2514751635995778
>5k	5.8508169986595136E-5	0.17243725607894728
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5175	0.1715469423705036	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	6.629833521565357E-5	3.3149167607826785E-5	0.0	3.3149167607826785E-5	0.0
6	6.629833521565357E-5	3.3149167607826785E-5	0.0	3.3149167607826785E-5	0.0
7	9.944750282348035E-5	3.3149167607826785E-5	0.0	3.3149167607826785E-5	0.0
8	9.944750282348035E-5	3.3149167607826785E-5	0.0	3.3149167607826785E-5	0.0
9	9.944750282348035E-5	3.3149167607826785E-5	0.0	3.3149167607826785E-5	0.0
10	9.944750282348035E-5	6.629833521565357E-5	0.0	3.3149167607826785E-5	0.0
11	9.944750282348035E-5	6.629833521565357E-5	0.0	6.629833521565357E-5	0.0
12	1.3259667043130714E-4	6.629833521565357E-5	0.0	9.944750282348035E-5	0.0
13	1.988950056469607E-4	6.629833521565357E-5	0.0	9.944750282348035E-5	3.3149167607826785E-5
14	1.988950056469607E-4	6.629833521565357E-5	0.0	9.944750282348035E-5	3.3149167607826785E-5
15	1.988950056469607E-4	6.629833521565357E-5	0.0	9.944750282348035E-5	6.629833521565357E-5
16	1.988950056469607E-4	6.629833521565357E-5	0.0	9.944750282348035E-5	6.629833521565357E-5
17	2.3204417325478749E-4	6.629833521565357E-5	0.0	9.944750282348035E-5	6.629833521565357E-5
18	2.3204417325478749E-4	6.629833521565357E-5	0.0	9.944750282348035E-5	6.629833521565357E-5
19	2.651933408626143E-4	6.629833521565357E-5	0.0	9.944750282348035E-5	6.629833521565357E-5
20	2.651933408626143E-4	6.629833521565357E-5	0.0	9.944750282348035E-5	1.3259667043130714E-4
21	2.651933408626143E-4	6.629833521565357E-5	0.0	1.3259667043130714E-4	1.3259667043130714E-4
22	3.3149167607826786E-4	9.944750282348035E-5	0.0	1.6574583803913393E-4	1.3259667043130714E-4
23	3.3149167607826786E-4	9.944750282348035E-5	0.0	1.988950056469607E-4	1.3259667043130714E-4
24	4.309391789017482E-4	9.944750282348035E-5	0.0	2.3204417325478749E-4	1.3259667043130714E-4
25	4.309391789017482E-4	1.3259667043130714E-4	0.0	2.9834250847044104E-4	1.6574583803913393E-4
26	4.309391789017482E-4	1.6574583803913393E-4	0.0	3.3149167607826786E-4	1.6574583803913393E-4
27	4.309391789017482E-4	1.6574583803913393E-4	0.0	4.6408834650957497E-4	1.6574583803913393E-4
28	4.6408834650957497E-4	1.6574583803913393E-4	0.0	8.618783578034964E-4	1.6574583803913393E-4
29	4.6408834650957497E-4	1.6574583803913393E-4	0.0	0.0014254142071365517	1.6574583803913393E-4
30	5.303866817252286E-4	1.6574583803913393E-4	0.0	0.0023204417325478747	1.6574583803913393E-4
31	5.303866817252286E-4	1.988950056469607E-4	0.0	0.004839778470742711	1.6574583803913393E-4
32	5.303866817252286E-4	1.988950056469607E-4	0.0	0.007823203555447121	1.6574583803913393E-4
33	5.303866817252286E-4	1.988950056469607E-4	0.0	0.011005523645798493	1.6574583803913393E-4
34	5.303866817252286E-4	1.988950056469607E-4	0.0	0.014386738741796825	1.988950056469607E-4
35	6.298341845487089E-4	1.988950056469607E-4	0.0	0.018198893016696905	1.988950056469607E-4
36	6.629833521565357E-4	2.3204417325478749E-4	0.0	0.02360220733677267	1.988950056469607E-4
37	6.629833521565357E-4	2.3204417325478749E-4	0.0	0.03039778669637716	1.988950056469607E-4
38	7.624308549800161E-4	2.3204417325478749E-4	0.0	0.03858563109551038	1.988950056469607E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7825	0.0	22.299267	1
GGTATCA	3860	0.0	20.065828	1
TATACCG	135	2.5956979E-9	19.55944	5
TCGATCG	95	3.3555966E-4	16.205952	41
TAGCGCG	140	1.5850383E-6	15.717146	4
TATCAAC	10995	0.0	15.609418	2
GCGTAAC	85	0.0029800127	15.530436	11
TCAACGC	11150	0.0	15.333733	4
ATCAACG	11155	0.0	15.287155	3
CAACGCA	11200	0.0	15.167298	5
GTACATG	9370	0.0	14.888515	1
AACGCAG	11500	0.0	14.730675	6
CGTATAC	90	0.004356223	14.669094	3
ATACCGT	150	3.2536664E-6	14.6669035	6
TACATGG	9555	0.0	14.43857	2
GCACCGT	325	0.0	14.215615	6
ACATGGG	9900	0.0	13.757833	3
ACCGTCC	195	2.4090878E-7	13.538681	8
CGTCCGA	185	1.9680392E-6	13.0819435	11
GTATTAT	685	0.0	12.849054	1
>>END_MODULE