FastQCFastQC Report
Mon 6 Feb 2017
SRR2077417_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077417_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741582
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27700.10103655480667732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17000.026.5289061
GTATCAA39800.025.31611
TATCAAC51750.019.0440042
TCAACGC52650.018.6349014
ATCAACG53250.018.5058633
CAACGCA54750.017.959995
AACGCAG56200.017.495976
CGTGCCG2400.017.41919510
GTATATA6100.015.5079011
GTACATG76150.015.2249231
GCGATAC1756.409937E-815.08735514
GTATTAC3250.014.8920241
TACATGG76600.014.7325932
ACGCAGA66900.014.534327
GCACCGT3700.014.26843556
CGCAGAG68250.014.1501298
TGCGATA1751.0524964E-613.83032813
ACATGGG80850.013.7120213
CTTACAC6450.013.6411573
GAGTACT30700.013.47402312
Produced by FastQC (version 0.11.5)