##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077417_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2741582 Sequences flagged as poor quality 0 Sequence length 50 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.181281464497506 31.0 28.0 34.0 15.0 34.0 2 28.2267201929397 31.0 28.0 34.0 15.0 34.0 3 28.27869201067121 31.0 28.0 34.0 15.0 34.0 4 31.582464431120425 35.0 32.0 37.0 16.0 37.0 5 31.650853412372857 35.0 32.0 37.0 16.0 37.0 6 31.638717353703083 35.0 32.0 37.0 15.0 37.0 7 31.463826724861775 35.0 32.0 37.0 11.0 37.0 8 31.371368793638126 35.0 32.0 37.0 10.0 37.0 9 32.483751717074306 38.0 32.0 39.0 10.0 39.0 10 32.374386394424825 37.0 32.0 39.0 10.0 39.0 11 32.44793079324273 37.0 32.0 39.0 10.0 39.0 12 32.35409008375456 37.0 32.0 39.0 8.0 39.0 13 32.36215185247058 37.0 32.0 39.0 8.0 39.0 14 33.12677133129704 38.0 32.0 41.0 8.0 41.0 15 33.225642348104124 38.0 32.0 41.0 8.0 41.0 16 33.11204698601027 38.0 32.0 41.0 8.0 41.0 17 32.91563447673642 38.0 32.0 41.0 7.0 41.0 18 32.71709545802387 38.0 32.0 40.0 7.0 41.0 19 33.076276762832556 39.0 32.0 41.0 2.0 41.0 20 33.11920234375627 39.0 32.0 41.0 2.0 41.0 21 33.11668481920293 39.0 32.0 41.0 2.0 41.0 22 33.01013101194857 39.0 32.0 41.0 2.0 41.0 23 32.73535900075212 38.0 32.0 40.0 2.0 41.0 24 32.89067735344046 38.0 32.0 41.0 2.0 41.0 25 32.78083931102553 38.0 32.0 41.0 2.0 41.0 26 32.81646509205269 38.0 32.0 41.0 2.0 41.0 27 32.800734758252716 38.0 32.0 41.0 2.0 41.0 28 32.66992269426922 38.0 32.0 40.0 2.0 41.0 29 32.627671176714756 38.0 32.0 40.0 2.0 41.0 30 32.39850713930862 38.0 31.0 40.0 2.0 41.0 31 32.331239043734605 38.0 31.0 40.0 2.0 41.0 32 32.34187888598627 38.0 31.0 40.0 2.0 41.0 33 32.32251597800102 38.0 31.0 40.0 2.0 41.0 34 32.29984767918669 38.0 31.0 40.0 2.0 41.0 35 32.235319607438335 38.0 31.0 40.0 2.0 41.0 36 32.10519364366997 38.0 31.0 40.0 2.0 41.0 37 32.00765725774389 38.0 30.0 40.0 2.0 41.0 38 31.895268498261224 38.0 30.0 40.0 2.0 41.0 39 31.830915507907477 38.0 30.0 40.0 2.0 41.0 40 31.787939955835718 38.0 30.0 40.0 2.0 41.0 41 31.665800621684852 38.0 30.0 40.0 2.0 41.0 42 31.470291970110686 38.0 30.0 40.0 2.0 41.0 43 31.355961630912372 38.0 30.0 40.0 2.0 41.0 44 31.276654500941426 38.0 30.0 40.0 2.0 41.0 45 31.182513964564986 38.0 30.0 40.0 2.0 41.0 46 31.014575526101353 38.0 30.0 40.0 2.0 41.0 47 30.821952070009214 37.0 30.0 40.0 2.0 41.0 48 30.62572850274039 37.0 29.0 40.0 2.0 41.0 49 30.4766364821479 37.0 29.0 40.0 2.0 41.0 50 30.263287401215795 37.0 29.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6474728961005063 1101 2 0.6677813787541673 1101 3 0.7833421098985234 1101 4 0.6613129362120489 1101 5 0.6226680905064583 1101 6 0.650173791341718 1101 7 0.7528179707444842 1101 8 0.7400829726809022 1101 9 0.9894439871700555 1101 10 0.9719033705466131 1101 11 0.9027927710783459 1101 12 0.9499177653403805 1101 13 0.8963197770528311 1101 14 1.1419452828453416 1101 15 1.1335926454088963 1101 16 1.0465714179807364 1101 17 1.2083868395569226 1101 18 1.3946020450997167 1101 19 0.9725847250351407 1101 20 0.9840640769516753 1101 21 0.9780123626542903 1101 22 0.8858468847120164 1101 23 1.0094907788558487 1101 24 0.8707097441503393 1101 25 0.9689853723361566 1101 26 0.8722587158977575 1101 27 0.966179495963047 1101 28 1.123825762351089 1101 29 0.9974491882191785 1101 30 1.1726379718878803 1101 31 1.0771154658506106 1101 32 1.1643454985677124 1101 33 1.0851125187484882 1101 34 1.0446904867245763 1101 35 1.122914237763183 1101 36 1.0684663769325766 1101 37 1.1476236730620926 1101 38 1.144330228182497 1101 39 1.0904572163172759 1101 40 1.0761739241572847 1101 41 1.1563940005430666 1101 42 1.359728487290461 1101 43 1.2809973463860693 1101 44 0.9698639603372996 1101 45 1.2002279633059274 1101 46 1.0495210072249748 1101 47 1.1238612898855713 1101 48 1.153062121580252 1101 49 1.127089670257245 1101 50 1.1044001641341161 1102 1 0.4329522961104644 1102 2 0.3682593339576101 1102 3 0.38821825603881166 1102 4 0.2627878822794507 1102 5 0.25093356295045055 1102 6 0.3976714084669801 1102 7 0.390467695029475 1102 8 0.4171210913589789 1102 9 0.6295574679912548 1102 10 0.624665765050004 1102 11 0.465190237261929 1102 12 0.460787666180309 1102 13 0.545548538322663 1102 14 0.8224204763473821 1102 15 0.6394457330083156 1102 16 0.7988424592807846 1102 17 0.8310225343155082 1102 18 0.9280109276047881 1102 19 0.7285609796385515 1102 20 0.6222778617920497 1102 21 0.734574809500316 1102 22 0.8439956261894608 1102 23 0.9950256984639836 1102 24 0.7940688416629698 1102 25 0.8508491626291388 1102 26 0.6985706028924099 1102 27 0.7281753910110211 1102 28 0.7204718360380653 1102 29 0.598212588643456 1102 30 0.864391877758834 1102 31 0.7983319423682076 1102 32 0.7414846912335094 1102 33 0.552825807975907 1102 34 0.5694669998165764 1102 35 0.5975490336896954 1102 36 0.728886037747813 1102 37 0.698057415460795 1102 38 0.8191129375519779 1102 39 0.8373330639177681 1102 40 0.7653370542656113 1102 41 0.8629935090411323 1102 42 0.8146877373213499 1102 43 0.7490408261084163 1102 44 0.7107068408858623 1102 45 0.7265805856937071 1102 46 0.7674815674874438 1102 47 0.7087555566422452 1102 48 0.6757534965702199 1102 49 0.7039150927121867 1102 50 0.4977120753427364 1103 1 0.2163791792724581 1103 2 0.18527925798227685 1103 3 0.2923648218841528 1103 4 0.2391858637883999 1103 5 0.2176977574924166 1103 6 0.27049455269361644 1103 7 0.2657785742615282 1103 8 0.2708469239953004 1103 9 0.42942103608582016 1103 10 0.4103989609191885 1103 11 0.40826038750939375 1103 12 0.3737862378814114 1103 13 0.4361321367536064 1103 14 0.6003585315879931 1103 15 0.4934776756029109 1103 16 0.629953082184457 1103 17 0.7386401381797683 1103 18 0.3566820779244111 1103 19 0.350623800834164 1103 20 0.5361675938450503 1103 21 0.49089018981666754 1103 22 0.5241364870192697 1103 23 0.7488128524213948 1103 24 0.5702910684945977 1103 25 0.3095072861850241 1103 26 0.4705341092566542 1103 27 0.5028138391579802 1103 28 0.5319686926995502 1103 29 0.5507850604243956 1103 30 0.698978271437042 1103 31 0.6139229247743359 1103 32 0.5850928856784776 1103 33 0.6450791342704889 1103 34 0.5209491265066006 1103 35 0.4404164642386945 1103 36 0.5843355867191207 1103 37 0.5819279214245086 1103 38 0.39149637372536006 1103 39 0.48295904826520086 1103 40 0.5174817667404774 1103 41 0.6496986142529195 1103 42 0.672002225717776 1103 43 0.8044995484455306 1103 44 0.5687161679201509 1103 45 0.575809891500711 1103 46 0.4741428041447975 1103 47 0.3871202902509161 1103 48 0.4245191968296176 1103 49 0.49465098654420814 1103 50 0.5944380894013577 1104 1 0.4074232396664037 1104 2 0.42916727193826887 1104 3 0.4328399075710365 1104 4 0.4596388816169039 1104 5 0.2578768417855386 1104 6 0.37482975414763686 1104 7 0.3944796697771764 1104 8 0.4273758879156766 1104 9 0.6206890524682436 1104 10 0.7209298372339603 1104 11 0.628714382809477 1104 12 0.5821803613222727 1104 13 0.5090094853446985 1104 14 0.8551803126447055 1104 15 0.7803587420359079 1104 16 0.7607990378382965 1104 17 0.9367691425789673 1104 18 0.9756096651048125 1104 19 0.7468447232108488 1104 20 0.6985628822020402 1104 21 0.5899365225445479 1104 22 0.636278983976176 1104 23 0.8807269545188774 1104 24 0.7268145125754586 1104 25 0.7724528335432339 1104 26 0.5514813482156811 1104 27 0.6455131901743556 1104 28 0.7913737229982942 1104 29 0.8267289673553009 1104 30 0.9552152588466356 1104 31 0.9914249214037838 1104 32 0.8452987526928979 1104 33 0.862602152288737 1104 34 0.7081756428956325 1104 35 0.7493436546806365 1104 36 0.7719185927432193 1104 37 0.7529846429503309 1104 38 0.8027096401996374 1104 39 0.6897789249772828 1104 40 0.9059735338423529 1104 41 0.857779774293185 1104 42 0.8737288430163801 1104 43 0.8678257922349921 1104 44 0.8006263601313108 1104 45 0.711677291156068 1104 46 0.8098043022867394 1104 47 0.8808574573427848 1104 48 0.8333382904144706 1104 49 0.655268771582108 1104 50 0.7493491078634982 1105 1 0.37285462050979845 1105 2 0.40071164675549653 1105 3 0.3365194487111651 1105 4 0.33871585149682204 1105 5 0.33296974818858516 1105 6 0.30871350933307795 1105 7 0.25169389446007884 1105 8 0.39213690905657117 1105 9 0.5458357525452087 1105 10 0.5559776020453953 1105 11 0.4607371158107938 1105 12 0.44304118125429426 1105 13 0.43772134138966123 1105 14 0.6739546918468662 1105 15 0.5819906573498201 1105 16 0.6664856197744555 1105 17 0.5517185004706207 1105 18 0.9263878645496106 1105 19 0.6533677404083349 1105 20 0.5551981139792446 1105 21 0.6352347669895977 1105 22 0.6178664711335031 1105 23 0.9835260428027652 1105 24 0.8183734656970572 1105 25 0.7447431181398727 1105 26 0.6549925254204894 1105 27 0.4020962267759245 1105 28 0.5554594375051565 1105 29 0.45945833755707355 1105 30 0.8185394487697693 1105 31 0.7844801362268043 1105 32 0.6961916738357772 1105 33 0.6574052829223902 1105 34 0.5009449789198257 1105 35 0.565130861242622 1105 36 0.6856925664316051 1105 37 0.4906704010709504 1105 38 0.5711446161498159 1105 39 0.5217902160961287 1105 40 0.5339240672191643 1105 41 0.7597521192632541 1105 42 0.7656466337638648 1105 43 0.49661208782121946 1105 44 0.4866648068755772 1105 45 0.5222683687088114 1105 46 0.4928636530137531 1105 47 0.47828744227651754 1105 48 0.7100412379443526 1105 49 0.6408523361929035 1105 50 0.6682589724151669 1106 1 0.1362197184268652 1106 2 0.12454697397658165 1106 3 0.21167528265420543 1106 4 0.23382635273501862 1106 5 0.2118997802301834 1106 6 0.26622938968585075 1106 7 0.1557420145855417 1106 8 0.19708810426454804 1106 9 -1.26545583839982 1106 10 -0.8634040462092969 1106 11 -0.10408016442414691 1106 12 0.18162830825978205 1106 13 0.2890890290505155 1106 14 0.40878208085835865 1106 15 0.32854696405517814 1106 16 0.4652675027814652 1106 17 0.5576044832663101 1106 18 -1.3434821197193472 1106 19 -0.1072975285590303 1106 20 -0.02410253996097822 1106 21 0.22680545678976927 1106 22 0.3290524202111058 1106 23 -1.274013516663782 1106 24 -0.059547295367607944 1106 25 0.17989979863615702 1106 26 0.32455564521741564 1106 27 0.41761479992150186 1106 28 0.5103261823782717 1106 29 0.3214645069800781 1106 30 -0.7591845551478684 1106 31 0.08909187909490157 1106 32 0.18570344419469365 1106 33 0.2726101549028215 1106 34 0.33660810657285367 1106 35 0.24717548900351716 1106 36 0.3494366298938445 1106 37 0.29706278670122543 1106 38 0.3434286068417194 1106 39 0.2856098377599565 1106 40 0.23339201293471135 1106 41 0.40919785403657016 1106 42 0.625018072409091 1106 43 0.4844626082353969 1106 44 0.3755518674753837 1106 45 0.27589843668459935 1106 46 0.3300542884949067 1106 47 0.28070584767380424 1106 48 0.37159695017874483 1106 49 0.41866041924731334 1106 50 0.39552289569527943 1107 1 0.18417488147108685 1107 2 0.20579962212548963 1107 3 0.19781279879926217 1107 4 0.27886115324049854 1107 5 0.148189854823638 1107 6 0.20963304491890966 1107 7 0.23986531883006634 1107 8 -4.1047348804568173E-4 1107 9 0.3275097079497513 1107 10 0.4326751618798639 1107 11 0.2795809244020262 1107 12 0.40228534677387984 1107 13 0.31801659836947493 1107 14 0.7313578207785554 1107 15 0.5910729059677493 1107 16 0.4324458260632298 1107 17 0.7090803994590686 1107 18 0.6563478319226093 1107 19 0.526364405058672 1107 20 0.4566485309349062 1107 21 0.4665371793436677 1107 22 0.605482159009199 1107 23 0.6291241044534317 1107 24 0.4754674056399608 1107 25 0.5290965545059052 1107 26 0.590671624083889 1107 27 0.47579101384059896 1107 28 0.5597782069947357 1107 29 0.5270348769693385 1107 30 0.7478622052074542 1107 31 0.7375474748358855 1107 32 0.7197638577980854 1107 33 0.5982263812659596 1107 34 0.5368398846649782 1107 35 0.33521088653134257 1107 36 0.5138558422625508 1107 37 0.5258148900958588 1107 38 0.4688613444862071 1107 39 0.4910354486341575 1107 40 0.6868892917794618 1107 41 0.33895349345600323 1107 42 0.4832175807731218 1107 43 0.47109299210539746 1107 44 0.45950839352259365 1107 45 0.47444897064189107 1107 46 0.517915757073947 1107 47 0.6136935068502396 1107 48 0.4467648102542334 1107 49 0.539039450497075 1107 50 0.6987127957820611 1108 1 -0.0026849694219848175 1108 2 0.12579503385270385 1108 3 0.05871423053462266 1108 4 0.01506867556614111 1108 5 0.21348603363778906 1108 6 0.07729083855548069 1108 7 0.11769197512139229 1108 8 0.09098291756513532 1108 9 0.10979198623988395 1108 10 0.058092608700306414 1108 11 0.2673810348005574 1108 12 0.020036265093125394 1108 13 0.06251611064845264 1108 14 -0.13390360957424718 1108 15 -0.031833633530979455 1108 16 0.28968564682324427 1108 17 0.0799216871641164 1108 18 0.36402808291892796 1108 19 0.2542970295067519 1108 20 0.1012995007094375 1108 21 0.30832712011989116 1108 22 0.25206323109256346 1108 23 0.12727004831665312 1108 24 0.27023851100294394 1108 25 0.3032641120857207 1108 26 0.19462299729109844 1108 27 0.23916804555258153 1108 28 0.21541856758218358 1108 29 0.2833640048019461 1108 30 0.3046517664310784 1108 31 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0.20586632091403345 2211 44 0.28807473450837406 2211 45 0.268004415340549 2211 46 0.11600792893504774 2211 47 0.23129533373093736 2211 48 0.3944074191675533 2211 49 0.26204498845989477 2211 50 0.34847384929220837 2212 1 -0.33208353438286053 2212 2 -0.2996028964844335 2212 3 -0.5374407542981743 2212 4 -0.47382958422877763 2212 5 -0.29878816632638205 2212 6 -0.3257400211623107 2212 7 -0.4797789430182178 2212 8 -0.4083684149928821 2212 9 -0.4847400015291825 2212 10 -0.3144095092425374 2212 11 -0.383252905726998 2212 12 -0.4523670914287692 2212 13 -0.5205710149918659 2212 14 -0.5804597130452507 2212 15 -0.5198782636728794 2212 16 -0.5786007020862058 2212 17 -0.2530910447847887 2212 18 -0.17600407337462798 2212 19 -0.3200678153748129 2212 20 -0.5824756906645376 2212 21 -0.449213971007822 2212 22 -0.3084374384506603 2212 23 -0.2090216994492593 2212 24 -0.32997168913126274 2212 25 -0.4815593471298385 2212 26 -0.4859118933501705 2212 27 -0.5684669486325618 2212 28 -0.6708972442655394 2212 29 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-0.1790934746284023 2216 10 -0.40852342095511673 2216 11 -0.3722647654710016 2216 12 -0.41039422127347436 2216 13 -0.5698195543822671 2216 14 -0.2819001036236486 2216 15 -0.4788784827029815 2216 16 -0.46713785534615937 2216 17 -0.3646961384168108 2216 18 -0.13228626711946845 2216 19 -0.47056112380443693 2216 20 -0.5074817463338661 2216 21 -0.6169088375399809 2216 22 -0.7349686194600835 2216 23 -2.345572860821804 2216 24 -1.2122775251905793 2216 25 -0.655944947618039 2216 26 -0.8107695014790011 2216 27 -0.9209349517248597 2216 28 -1.2128528842014639 2216 29 -0.7981413075025046 2216 30 -0.732644354339488 2216 31 -0.45541741114145395 2216 32 -0.5839780209929373 2216 33 -0.6064282245175683 2216 34 -0.7603942575328517 2216 35 -0.9476022350288993 2216 36 -0.7720563582564886 2216 37 -0.689781659028192 2216 38 -0.7476164548223316 2216 39 -0.842233472841837 2216 40 -0.8948196974578764 2216 41 -1.0264337692117387 2216 42 -1.1889133801578566 2216 43 -1.0765444310014374 2216 44 -1.1294813861961082 2216 45 -1.024276807323794 2216 46 -1.0584912788435972 2216 47 -1.0366618194960715 2216 48 -1.1195652946928263 2216 49 -1.4392598543849644 2216 50 -1.3577364316690428 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 199896.0 3 9551.0 4 17957.0 5 13627.0 6 10680.0 7 9005.0 8 8073.0 9 7083.0 10 6861.0 11 6814.0 12 6816.0 13 7063.0 14 7576.0 15 8000.0 16 8278.0 17 9360.0 18 10210.0 19 11413.0 20 14241.0 21 18924.0 22 23265.0 23 26453.0 24 28138.0 25 29768.0 26 32601.0 27 36826.0 28 41443.0 29 46727.0 30 53693.0 31 63760.0 32 76627.0 33 93269.0 34 116496.0 35 148903.0 36 192879.0 37 260945.0 38 409363.0 39 668998.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.79637067708552 17.569149295322283 12.88971697923833 24.744763048353864 2 15.863697582661446 19.704904390482483 39.126465897021305 25.304932129834768 3 18.814122380631545 24.949359059052995 28.74445504507865 27.492063515236808 4 12.84575501038141 16.340282502869126 37.08255338907075 33.73140909767871 5 14.206201651695446 37.349175922758796 34.1176264443574 14.326995981188354 6 32.430304709201934 36.641421136826246 17.360325647991107 13.567948505980713 7 28.628429837717906 31.906185111794738 21.347667582628116 18.11771746785924 8 26.73559332178595 34.52460461880852 19.74332977860697 18.996472280798564 9 27.142559962628283 15.803455424008408 18.656223996729977 38.397760616633335 10 15.012429816521966 28.65657608650028 33.16233438925637 23.16865970772138 11 34.99795445645656 23.205478042248775 23.2147842617595 18.581783239535156 12 23.72568751316297 24.776790684211697 29.73212692079003 21.765394881835302 13 28.175561271286625 21.952349198319443 25.56928279984685 24.302806730547076 14 22.757288342958173 21.007699141465793 26.489769638942605 29.745242876633434 15 23.90721403368394 28.67818034609665 23.390580499995252 24.02402512022416 16 23.958116222882257 28.003187348722303 24.67030542261755 23.36839100577789 17 23.01254023436231 27.63630217324879 25.998781424054574 23.35237616833433 18 23.091489788841642 27.23205518953151 26.779479130545873 22.896975891080977 19 23.819759486107507 26.879564568165254 26.855182487826294 22.445493457900945 20 24.048561388753214 26.376754139078283 26.80477101006141 22.76991346210709 21 24.239017192702974 26.22060598273064 26.65577140837944 22.884605416186943 22 24.070632038108446 25.88034531218631 26.593236115420417 23.45578653428483 23 23.98996498769167 26.032738990395703 26.41545797792969 23.561838043982934 24 23.45196840047958 26.18241806771378 26.59056941547457 23.775044116332076 25 23.824780396920882 25.9899295050754 26.594714048756558 23.590576049247158 26 23.41386270470483 26.500986219215562 26.704582168324237 23.38056890775537 27 23.600244576872882 26.38006150925833 26.532757786761817 23.486936127106965 28 23.765619158332555 26.182974168146554 26.54596077904477 23.505445894476118 29 23.624059195135413 26.35005680606788 26.56906683255791 23.456817166238796 30 23.87455329276617 26.29602590263151 26.520702758542647 23.308718046059667 31 23.938925502034053 25.995572354408758 26.417786505718887 23.647715637838303 32 23.56823522239732 26.3860795743884 26.506105901330912 23.53957930188337 33 23.853567670033367 26.146596594897492 26.347510856999346 23.65232487806979 34 23.888979121081665 26.184327945558966 26.44583908943754 23.480853843921828 35 23.853190579846906 26.289379165433107 26.391410259545932 23.466019995174054 36 23.93502626132676 26.264283828958757 26.2270101588092 23.57367975090528 37 23.99842566717465 25.979814073922082 26.4830018058094 23.538758453093862 38 23.951491205207446 26.111477363073483 26.476074499299497 23.460956932419577 39 23.953408336529733 26.2830280055809 26.303210038024698 23.46035361986467 40 23.881182062415622 26.09255807553716 26.554176887452673 23.472082974594542 41 23.77734518416007 26.188458509333003 26.43327048804005 23.60092581846688 42 24.05079738166104 26.12659927990204 26.356242278473157 23.46636105996376 43 23.90049972723906 26.051425016283552 26.300394636735337 23.747680619742052 44 23.824006562224735 26.320162420900452 26.233281542940173 23.622549473934644 45 23.847322662077634 26.273570068521696 26.045359040097853 23.83374822930282 46 23.909253257297493 26.081587703095316 26.07706291858818 23.93209612101901 47 23.814784801369846 26.273603510283877 26.216173911710932 23.695437776635348 48 23.845573912389806 26.44238032438003 26.08286630326022 23.629179459969947 49 24.00327712707626 26.1291034794842 26.05465649467832 23.812962898761224 50 24.101114780419273 26.317466109580543 25.9222755386889 23.65914357131128 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1883.0 1 1594.5 2 1306.0 3 1217.5 4 1129.0 5 1103.0 6 1077.0 7 1018.0 8 959.0 9 1084.0 10 1209.0 11 1521.0 12 1833.0 13 3176.0 14 4519.0 15 6442.5 16 8366.0 17 9200.0 18 10034.0 19 10576.5 20 11119.0 21 12946.5 22 14774.0 23 18467.0 24 22160.0 25 27335.0 26 32510.0 27 38322.0 28 44134.0 29 50458.0 30 56782.0 31 63023.5 32 69265.0 33 78143.5 34 87022.0 35 96234.0 36 105446.0 37 112884.0 38 120322.0 39 128819.0 40 137316.0 41 145840.5 42 154365.0 43 160320.5 44 166276.0 45 173341.5 46 180407.0 47 185405.5 48 190404.0 49 192766.5 50 195129.0 51 195521.0 52 195913.0 53 192555.0 54 189197.0 55 181108.5 56 173020.0 57 162683.5 58 152347.0 59 138779.5 60 125212.0 61 110325.0 62 95438.0 63 82294.5 64 69151.0 65 57991.5 66 46832.0 67 38512.0 68 30192.0 69 24487.5 70 18783.0 71 14698.0 72 10613.0 73 8589.5 74 6566.0 75 5114.0 76 3662.0 77 2862.5 78 2063.0 79 1620.5 80 1178.0 81 921.5 82 665.0 83 520.5 84 376.0 85 303.5 86 231.0 87 196.0 88 161.0 89 126.0 90 91.0 91 68.5 92 46.0 93 36.0 94 26.0 95 21.5 96 17.0 97 16.5 98 16.0 99 28.0 100 40.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.061169062242165284 2 0.06536372065471688 3 0.06215389508685131 4 0.07550385142592853 5 0.08079276855479792 6 0.06167971630978026 7 0.047126075382753464 8 0.05719325557287726 9 0.05624489801873517 10 0.06561904768852436 11 0.053946954714467775 12 0.06890182383747778 13 0.06474364071547012 14 0.06550962181689259 15 0.07718171479094917 16 0.07298705637839759 17 0.0845861987713663 18 0.07047026133086663 19 0.06802641686442354 20 0.08039154035881473 21 0.07893252873705765 22 0.07411779038525931 23 0.07200222353371155 24 0.060804309336726016 25 0.07637925839898277 26 0.08509685283898129 27 0.07867720170325017 28 0.09622181645487897 29 0.0889267583460936 30 0.07630630781789492 31 0.07211164940534334 32 0.07962555925739226 33 0.07678048659496597 34 0.06335757967480089 35 0.08101162029806148 36 0.08692061736617764 37 0.09644066819814254 38 0.07732761595312487 39 0.05547891691731271 40 0.06051250701237461 41 0.055442441626768785 42 0.061169062242165284 43 0.039648640821248464 44 0.038226104490035315 45 0.04143593005790088 46 0.040852325409198045 47 0.030456867604178903 48 0.044353953301415026 49 0.05022647507898724 50 0.04129002889572517 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2741582.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.37710471593218 #Duplication Level Percentage of deduplicated Percentage of total 1 78.5585670297827 48.216973949205496 2 11.541940046966207 14.168217257753119 3 4.153286043145007 7.647500171559921 4 1.97901402160409 4.858646033531691 5 1.1021761615289443 3.3824190840783093 6 0.6536529509423048 2.407159537071835 7 0.42248719543227986 1.815172858463128 8 0.29261172101547933 1.4367728193500966 9 0.21830912001448385 1.2059263547614782 >10 1.0094780013780065 10.818986622093306 >50 0.051748016968374555 2.13799356950699 >100 0.016131063103143013 1.5330267030121354 >500 3.5917687137345473E-4 0.12293893524406255 >1k 2.394512475125034E-4 0.24826610436841315 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2770 0.10103655480667732 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.647529054392683E-5 0.0 2 0.0 0.0 0.0 3.647529054392683E-5 0.0 3 0.0 0.0 0.0 3.647529054392683E-5 0.0 4 0.0 0.0 0.0 7.295058108785365E-5 0.0 5 0.0 0.0 0.0 7.295058108785365E-5 0.0 6 0.0 0.0 0.0 7.295058108785365E-5 0.0 7 3.647529054392683E-5 0.0 0.0 7.295058108785365E-5 0.0 8 7.295058108785365E-5 0.0 0.0 7.295058108785365E-5 0.0 9 7.295058108785365E-5 0.0 0.0 7.295058108785365E-5 0.0 10 1.0942587163178048E-4 0.0 0.0 7.295058108785365E-5 0.0 11 1.0942587163178048E-4 0.0 0.0 7.295058108785365E-5 0.0 12 1.0942587163178048E-4 0.0 0.0 7.295058108785365E-5 0.0 13 1.0942587163178048E-4 0.0 0.0 7.295058108785365E-5 0.0 14 1.0942587163178048E-4 0.0 0.0 7.295058108785365E-5 0.0 15 1.0942587163178048E-4 0.0 0.0 7.295058108785365E-5 0.0 16 1.0942587163178048E-4 0.0 0.0 1.8237645271963415E-4 0.0 17 1.0942587163178048E-4 0.0 0.0 1.8237645271963415E-4 0.0 18 1.0942587163178048E-4 0.0 0.0 1.8237645271963415E-4 0.0 19 1.459011621757073E-4 0.0 0.0 1.8237645271963415E-4 0.0 20 1.459011621757073E-4 0.0 0.0 1.8237645271963415E-4 0.0 21 1.459011621757073E-4 0.0 0.0 1.8237645271963415E-4 0.0 22 2.1885174326356096E-4 0.0 0.0 2.1885174326356096E-4 0.0 23 2.1885174326356096E-4 0.0 0.0 2.553270338074878E-4 0.0 24 2.1885174326356096E-4 0.0 0.0 3.2827761489534143E-4 0.0 25 2.1885174326356096E-4 0.0 0.0 3.647529054392683E-4 0.0 26 2.1885174326356096E-4 0.0 0.0 5.106540676149756E-4 0.0 27 2.1885174326356096E-4 0.0 0.0 5.471293581589024E-4 0.0 28 2.1885174326356096E-4 0.0 0.0 7.295058108785366E-4 0.0 29 2.553270338074878E-4 0.0 0.0 0.0011672092974056585 0.0 30 2.553270338074878E-4 0.0 0.0 0.0017508139461084877 0.0 31 2.553270338074878E-4 0.0 0.0 0.003756954926024463 3.647529054392683E-5 32 2.553270338074878E-4 0.0 0.0 0.006018422939747927 3.647529054392683E-5 33 2.918023243514146E-4 0.0 0.0 0.008498742696734951 3.647529054392683E-5 34 2.918023243514146E-4 0.0 0.0 0.011562667102424805 3.647529054392683E-5 35 3.2827761489534143E-4 0.0 0.0 0.015210196156817487 3.647529054392683E-5 36 3.2827761489534143E-4 0.0 0.0 0.019623706312632633 3.647529054392683E-5 37 3.2827761489534143E-4 0.0 0.0 0.02483967286041417 3.647529054392683E-5 38 4.012281959831951E-4 3.647529054392683E-5 0.0 0.03187940393539205 3.647529054392683E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1700 0.0 26.528906 1 GTATCAA 3980 0.0 25.3161 1 TATCAAC 5175 0.0 19.044004 2 TCAACGC 5265 0.0 18.634901 4 ATCAACG 5325 0.0 18.505863 3 CAACGCA 5475 0.0 17.95999 5 AACGCAG 5620 0.0 17.49597 6 CGTGCCG 240 0.0 17.419195 10 GTATATA 610 0.0 15.507901 1 GTACATG 7615 0.0 15.224923 1 GCGATAC 175 6.409937E-8 15.087355 14 GTATTAC 325 0.0 14.892024 1 TACATGG 7660 0.0 14.732593 2 ACGCAGA 6690 0.0 14.53432 7 GCACCGT 370 0.0 14.2684355 6 CGCAGAG 6825 0.0 14.150129 8 TGCGATA 175 1.0524964E-6 13.830328 13 ACATGGG 8085 0.0 13.712021 3 CTTACAC 645 0.0 13.641157 3 GAGTACT 3070 0.0 13.474023 12 >>END_MODULE