FastQCFastQC Report
Mon 6 Feb 2017
SRR2077417_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077417_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741582
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32010.11675740503110978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18000.031.9014281
GTATCAA45700.025.8042181
TATCAAC68000.017.1472022
ATCAACG71350.016.3427093
TCAACGC71950.016.1455634
CAACGCA73150.015.7609665
AACGCAG74450.015.5440146
GCGATAC2001.2769306E-915.40209414
GCACCGT4000.015.4006896
ACCGTAT1901.0764779E-815.0541318
TAATACC4550.014.9899554
GTGCCGA2355.0931703E-1114.98021411
ACCGTGC5200.014.8091668
TAGATCG1057.157518E-414.6681275
CGTGCCG2701.8189894E-1214.66812510
GCGCGTT1201.18308686E-414.66491536
TGCGATA2305.9480953E-1014.34977913
ATAATAC5900.014.1701263
CGCGTTG1251.6749756E-414.07729137
AGCACCG4400.014.0013935
Produced by FastQC (version 0.11.5)