FastQCFastQC Report
Mon 6 Feb 2017
SRR2077416_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077416_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3102295
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37890.12213538686681956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25500.025.4515291
GTATCAA56900.024.3977151
TATCAAC74650.018.5369762
TCAACGC74250.018.3707684
ATCAACG74700.018.230063
CAACGCA75650.018.058715
AACGCAG77750.017.57016
ACCGTCG1105.626306E-515.999838
CGTACAC2650.015.7734173
CGTGCCG1401.5855912E-615.71665510
TCGATCG1004.960553E-415.39437341
GTACATG97000.014.9920771
ACGCAGA91050.014.9078927
CGCGCGT1503.2486532E-614.66911532
CGCAGAG92750.014.5637268
TACATGG97450.014.5385842
AGAGTAC90000.014.17968611
ACATGGG100750.013.909533
GAGTACT44800.013.89919112
CAGAGTA96050.013.72200610
Produced by FastQC (version 0.11.5)