##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077416_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3102295
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.667779498725942	31.0	30.0	34.0	16.0	34.0
2	28.617913512415807	31.0	30.0	34.0	16.0	34.0
3	28.729796167031182	31.0	30.0	34.0	16.0	34.0
4	32.0454998638105	35.0	33.0	37.0	18.0	37.0
5	32.12759586048393	35.0	33.0	37.0	19.0	37.0
6	32.135581239050445	36.0	33.0	37.0	19.0	37.0
7	31.96758625469209	36.0	33.0	37.0	17.0	37.0
8	31.900960740355124	36.0	33.0	37.0	17.0	37.0
9	33.06927967843161	38.0	33.0	39.0	15.0	39.0
10	32.9593546068314	38.0	33.0	39.0	13.0	39.0
11	33.02309580487994	38.0	33.0	39.0	13.0	39.0
12	32.941191279359316	38.0	33.0	39.0	13.0	39.0
13	32.937705150541774	38.0	33.0	39.0	12.0	39.0
14	33.75555580626601	39.0	33.0	41.0	10.0	41.0
15	33.84947756419038	39.0	33.0	41.0	11.0	41.0
16	33.75029647406195	39.0	33.0	41.0	10.0	41.0
17	33.567313231011234	39.0	33.0	41.0	10.0	41.0
18	33.38199655416393	38.0	32.0	41.0	10.0	41.0
19	33.73437342354612	39.0	33.0	41.0	10.0	41.0
20	33.77139117975563	39.0	33.0	41.0	10.0	41.0
21	33.770422219679304	39.0	33.0	41.0	10.0	41.0
22	33.67308041304905	39.0	33.0	41.0	10.0	41.0
23	33.408844097676074	39.0	32.0	41.0	10.0	41.0
24	33.56597809041371	39.0	33.0	41.0	10.0	41.0
25	33.46358228343855	39.0	33.0	41.0	9.0	41.0
26	33.498088350721	39.0	33.0	41.0	8.0	41.0
27	33.47738722461919	39.0	33.0	41.0	8.0	41.0
28	33.36053985839516	39.0	33.0	41.0	8.0	41.0
29	33.31907668355201	39.0	33.0	41.0	7.0	41.0
30	33.092311337251935	38.0	32.0	41.0	2.0	41.0
31	33.01969896479864	38.0	32.0	41.0	2.0	41.0
32	33.02430071930619	38.0	32.0	41.0	2.0	41.0
33	32.99366630188296	38.0	32.0	41.0	2.0	41.0
34	32.97854201486319	38.0	32.0	41.0	2.0	41.0
35	32.90787239769268	38.0	32.0	40.0	2.0	41.0
36	32.774385737010824	38.0	32.0	40.0	2.0	41.0
37	32.67603113179114	38.0	32.0	40.0	2.0	41.0
38	32.558237047089335	38.0	31.0	40.0	2.0	41.0
39	32.49814669462447	38.0	31.0	40.0	2.0	41.0
40	32.45097452047597	38.0	31.0	40.0	2.0	41.0
41	32.32647991245191	38.0	31.0	40.0	2.0	41.0
42	32.14129668519596	38.0	31.0	40.0	2.0	41.0
43	32.02670055555645	38.0	31.0	40.0	2.0	41.0
44	31.94815934654828	38.0	31.0	40.0	2.0	41.0
45	31.857465843834966	38.0	31.0	40.0	2.0	41.0
46	31.687873654826507	38.0	31.0	40.0	2.0	41.0
47	31.496896007633058	38.0	30.0	40.0	2.0	41.0
48	31.29810801358349	38.0	30.0	40.0	2.0	41.0
49	31.165426240895854	38.0	30.0	40.0	2.0	41.0
50	30.961224835162355	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5406759023269032
1101	2	0.44678764922230485
1101	3	0.6239906603158261
1101	4	0.5739626149219887
1101	5	0.3965119921283673
1101	6	0.5756215029782794
1101	7	0.5899855798313709
1101	8	0.5232402051357958
1101	9	0.770787410402022
1101	10	0.7669812978587345
1101	11	0.7401088962215923
1101	12	0.7333502707106163
1101	13	0.7081781687346833
1101	14	1.0555166300801062
1101	15	1.0277135850255092
1101	16	0.7927110871790504
1101	17	1.0836918712483765
1101	18	1.2511117302598649
1101	19	0.8505615819002159
1101	20	0.9138955826082977
1101	21	0.9910252274086915
1101	22	0.8591260956571602
1101	23	0.8262566863101029
1101	24	0.852142636862439
1101	25	0.8609904170127649
1101	26	0.8964187379466964
1101	27	0.8862174301609116
1101	28	0.9363129633246174
1101	29	0.8789641429536914
1101	30	1.0582657809147094
1101	31	1.0828682240916763
1101	32	1.0298270399430862
1101	33	1.056371879136158
1101	34	0.9285262862468002
1101	35	0.9351534351625972
1101	36	0.940769870097256
1101	37	0.9687436086828853
1101	38	0.9714853819877192
1101	39	1.005879399727199
1101	40	0.8353370240083464
1101	41	0.8881562791478856
1101	42	1.0433579032760107
1101	43	1.1632921981087563
1101	44	0.9627768179606306
1101	45	1.1129689758262202
1101	46	1.1083678951782083
1101	47	1.1418093021077738
1101	48	1.1265495530168046
1101	49	1.2697236700212429
1101	50	1.2669051472274475
1102	1	0.43960530331919756
1102	2	0.4401252009261256
1102	3	0.3932359636196807
1102	4	0.47188034433627735
1102	5	0.39801144554921564
1102	6	0.4967842031351424
1102	7	0.5861720767699836
1102	8	0.5283071833785122
1102	9	0.7769306315437206
1102	10	0.7177870053562856
1102	11	0.46677431989485285
1102	12	0.4479114722598325
1102	13	0.5130855457559989
1102	14	0.9545000302627358
1102	15	0.8520808389406014
1102	16	0.9166348917191129
1102	17	0.9585213685338019
1102	18	0.9809609917369002
1102	19	0.8862412758354026
1102	20	0.7667179701087647
1102	21	0.7134015647899901
1102	22	0.8273619393980312
1102	23	1.0118084764401871
1102	24	0.8226036159143888
1102	25	0.766900948269921
1102	26	0.6656559088423677
1102	27	0.7371851406510785
1102	28	0.7797840573032104
1102	29	0.6287828580212675
1102	30	0.9411516212589461
1102	31	1.000558060657923
1102	32	0.7900886887910161
1102	33	0.6162955559431964
1102	34	0.5779342659066771
1102	35	0.7125135806481282
1102	36	0.6376180360304815
1102	37	0.6850506756209
1102	38	0.7697874002182985
1102	39	0.7137509365208459
1102	40	0.6850041997818437
1102	41	0.7533459450690927
1102	42	0.673485352750582
1102	43	0.80719459164456
1102	44	0.6279279471102654
1102	45	0.6539248948666909
1102	46	0.6419448465454884
1102	47	0.6736828934730212
1102	48	0.6603339350088113
1102	49	0.8242843489276837
1102	50	0.8781526689438266
1103	1	0.3903856683857292
1103	2	0.4042227393098763
1103	3	0.44401993118190575
1103	4	0.3809507835098671
1103	5	0.3510253579471083
1103	6	0.4308741584489937
1103	7	0.4849199761662817
1103	8	0.53679266010327
1103	9	0.5983938813944221
1103	10	0.6417305079459155
1103	11	0.6725529642059769
1103	12	0.4985538570217898
1103	13	0.6003597008698165
1103	14	0.8028241592283081
1103	15	0.6064747955993752
1103	16	0.8613290841447139
1103	17	1.0570639414068665
1103	18	0.7666214123122828
1103	19	0.687372186805888
1103	20	0.8176103271703141
1103	21	0.7543867964978475
1103	22	0.7440207447234144
1103	23	0.9713892613049637
1103	24	0.8863189174440649
1103	25	0.6284816845770393
1103	26	0.6640560756373972
1103	27	0.660899541294647
1103	28	0.9343299165752583
1103	29	0.8113699774578436
1103	30	0.9787986451648862
1103	31	0.919076477407728
1103	32	0.7179902172192953
1103	33	0.8182991832945419
1103	34	0.7775511743119878
1103	35	0.6959397407343317
1103	36	0.8088221080776705
1103	37	0.7948384361863461
1103	38	0.7238284696131174
1103	39	0.8724084651888688
1103	40	0.8119156659068736
1103	41	0.9043890458624659
1103	42	0.8398655939164286
1103	43	0.8997308550636589
1103	44	0.7310817669119629
1103	45	0.6601239142432362
1103	46	0.7125553353244491
1103	47	0.7482521422236772
1103	48	0.7481251295339852
1103	49	0.7530271413799383
1103	50	0.7715674064443334
1104	1	0.8233669982412444
1104	2	0.7875074483556368
1104	3	0.7797770314219008
1104	4	0.824699822400774
1104	5	0.7307398516623138
1104	6	0.8356787229172369
1104	7	0.8240807765363343
1104	8	0.8855449825588799
1104	9	1.1458230294847809
1104	10	1.146704032439935
1104	11	1.0044154475095155
1104	12	0.9233274564425074
1104	13	0.9476869880886056
1104	14	1.2323398465318718
1104	15	1.1583481840563294
1104	16	1.2370982153904322
1104	17	1.3093226569264829
1104	18	1.4574775706062937
1104	19	1.2691897541492665
1104	20	1.2425891686456865
1104	21	1.1353114273626161
1104	22	1.2527135880726732
1104	23	1.4323291199385935
1104	24	1.3347513375940707
1104	25	1.358904463692113
1104	26	1.209454477892713
1104	27	1.1676125384275196
1104	28	1.1849044761959675
1104	29	1.3303190169240011
1104	30	1.401054434084834
1104	31	1.5023458908869571
1104	32	1.3857736451457967
1104	33	1.25441769650795
1104	34	1.2065339307949614
1104	35	1.1879233332859087
1104	36	1.3178019102255618
1104	37	1.285938244945541
1104	38	1.3373227546285875
1104	39	1.1338735400893825
1104	40	1.2947593613445108
1104	41	1.4096990933650133
1104	42	1.428486401208005
1104	43	1.357782532712008
1104	44	1.3084050674219725
1104	45	1.3186605096309236
1104	46	1.31744763614849
1104	47	1.3352020963421083
1104	48	1.3369788629311294
1104	49	1.295782872415053
1104	50	1.3496586183555195
1105	1	0.6216710286207245
1105	2	0.6039493524068256
1105	3	0.5677075789465427
1105	4	0.5972038391866619
1105	5	0.5517996891651151
1105	6	0.47968754798668556
1105	7	0.47308275398492583
1105	8	0.61568902476996
1105	9	0.7719421336393779
1105	10	0.7895693616329993
1105	11	0.714951734518408
1105	12	0.8329177333191922
1105	13	0.8368878784088736
1105	14	1.0725052537983828
1105	15	0.8216341929647157
1105	16	0.8817570212953143
1105	17	0.8578354246349704
1105	18	1.1233180580141848
1105	19	0.9777778925272145
1105	20	0.8748510999597912
1105	21	0.8678278697251045
1105	22	0.7777455240369022
1105	23	1.1334759643822991
1105	24	0.9505892438146475
1105	25	0.9674265475932557
1105	26	0.9021020405841753
1105	27	0.870578671456137
1105	28	0.9564061152997425
1105	29	0.9063339866144844
1105	30	1.0843905877888602
1105	31	1.1357226160718312
1105	32	1.0056536508303822
1105	33	0.9340550785581527
1105	34	0.8606975852459371
1105	35	0.8610398787762961
1105	36	0.9635982324223136
1105	37	0.9277009387563311
1105	38	0.8478896770420832
1105	39	0.8899216406643973
1105	40	0.8564768455100733
1105	41	0.9818872286335818
1105	42	0.9996993659039433
1105	43	0.9898466394388166
1105	44	0.8863909081645147
1105	45	0.9670658242059034
1105	46	0.7899658644135421
1105	47	0.9003931211452283
1105	48	1.0764706899353733
1105	49	0.935236151031205
1105	50	0.940542622189632
1106	1	0.5694287896199448
1106	2	0.5470880423192312
1106	3	0.5413122186797352
1106	4	0.5866630292571173
1106	5	0.6286598359239548
1106	6	0.49467485892526497
1106	7	0.6325033101583202
1106	8	0.5971322913199266
1106	9	-0.7548185272895509
1106	10	-0.33707479278953656
1106	11	0.2940983142305953
1106	12	0.6718265077986558
1106	13	0.7266058560727728
1106	14	0.869265354284515
1106	15	0.8591273333282388
1106	16	0.9413484884043157
1106	17	0.951912113465653
1106	18	-0.9035497236924783
1106	19	0.23926766907645458
1106	20	0.4125534176367083
1106	21	0.5813249816119637
1106	22	0.8222121257462263
1106	23	-0.7116981457073805
1106	24	0.44818657444782417
1106	25	0.5948035219603796
1106	26	0.6725052208124467
1106	27	0.8157120457471265
1106	28	0.8929661618251714
1106	29	0.8974474957648013
1106	30	-0.0477639925576554
1106	31	0.6816912719824302
1106	32	0.8003162387082483
1106	33	0.8630462053307966
1106	34	0.7848452708921272
1106	35	0.8617230691484892
1106	36	0.9128317135639961
1106	37	0.8259068273026813
1106	38	0.9594396409026871
1106	39	0.9697918282236628
1106	40	0.9438534456055834
1106	41	1.035047542377363
1106	42	1.0931944868201953
1106	43	0.8887259274893395
1106	44	0.9062683959493185
1106	45	0.955234356008809
1106	46	0.9218148539236175
1106	47	0.9349122009425415
1106	48	1.0627701305633046
1106	49	1.0165371557864802
1106	50	1.0448839991792411
1107	1	0.3386327333798711
1107	2	0.5194833922125532
1107	3	0.4561717437008781
1107	4	0.39249986864684416
1107	5	0.3498983251443448
1107	6	0.39717869178444687
1107	7	0.43876343697760944
1107	8	0.22728051407154126
1107	9	0.5280868712238203
1107	10	0.6159753878503551
1107	11	0.43470373126279327
1107	12	0.5328381279723189
1107	13	0.5431908291143728
1107	14	0.7372892287141539
1107	15	0.6697139896164401
1107	16	0.584676625431733
1107	17	0.8782499146600529
1107	18	0.7657722510508975
1107	19	0.7216225109852488
1107	20	0.5880704274392343
1107	21	0.6204923673155136
1107	22	0.7103728527367252
1107	23	0.9325549422607153
1107	24	0.7023982631510464
1107	25	0.7491482377926175
1107	26	0.6992164684733666
1107	27	0.6310632321332292
1107	28	0.6609890883852287
1107	29	0.6548128314386616
1107	30	0.8482774918740148
1107	31	0.932829741156084
1107	32	0.897729219683093
1107	33	0.6649805100465329
1107	34	0.6166850022743802
1107	35	0.6385781595547328
1107	36	0.7712345703014307
1107	37	0.5762453369942264
1107	38	0.6440564108659146
1107	39	0.6644073489796725
1107	40	0.7444245158055125
1107	41	0.6841528737476139
1107	42	0.7912782199210611
1107	43	0.7036780183410798
1107	44	0.7290353177909665
1107	45	0.6434514741655661
1107	46	0.7132637404810289
1107	47	0.8026794432206898
1107	48	0.7558300134264364
1107	49	0.7242237980264044
1107	50	0.7627455261858138
1108	1	0.0281865532741854
1108	2	0.16375791971904619
1108	3	0.09695551582220219
1108	4	-0.002151477647153399
1108	5	0.03569596386834917
1108	6	0.0312794772512035
1108	7	0.07077332100653422
1108	8	0.05971142712711597
1108	9	0.12747636452626665
1108	10	0.015449959841006944
1108	11	0.030772526120159682
1108	12	-0.007136574859451628
1108	13	0.12576207909177128
1108	14	-0.041702261043077726
1108	15	-0.012152206570007706
1108	16	0.02242263733398886
1108	17	0.00491854987846807
1108	18	0.3014332142132332
1108	19	0.09580694529232403
1108	20	-0.057950139726457905
1108	21	0.10095718474877202
1108	22	0.07492722503076976
1108	23	-0.014268503665377352
1108	24	0.11632210539882948
1108	25	0.24218973606867422
1108	26	0.14548441027947234
1108	27	0.05941342689750684
1108	28	0.15759571057139254
1108	29	0.1116207882590814
1108	30	0.23296729066720445
1108	31	0.23348630246871238
1108	32	0.17151071263054263
1108	33	0.22672364253097754
1108	34	0.13561971264435613
1108	35	0.053653869360985595
1108	36	0.1383863169711006
1108	37	0.06320897740519626
1108	38	0.036374804206296574
1108	39	0.2564027665272164
1108	40	0.17042856393678818
1108	41	0.09506883101433772
1108	42	0.15110195053845388
1108	43	0.29028005150077263
1108	44	0.2723169319011376
1108	45	0.1754298029308714
1108	46	0.17202244442892578
1108	47	0.20858559151180245
1108	48	0.2612780899523308
1108	49	0.1210872997804806
1108	50	0.2254019808533343
1109	1	-0.12971669037061773
1109	2	-0.21350511206163603
1109	3	-0.13981569006003625
1109	4	-0.1303654370801688
1109	5	-0.25774265311157407
1109	6	-0.23419639020356087
1109	7	-0.2660434575677897
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2211	4	0.14485839222295027
2211	5	-0.07761764559910489
2211	6	-0.1369041809107827
2211	7	-0.01355096792229915
2211	8	-0.05139247438848926
2211	9	0.01371926572041815
2211	10	-0.16727955333271183
2211	11	0.043194989896903735
2211	12	0.024665480073693402
2211	13	-0.027895963840379068
2211	14	0.08619232541055766
2211	15	-0.09143440865828012
2211	16	-0.18421005824778547
2211	17	0.18312387651283046
2211	18	0.3623555529795581
2211	19	0.1001001602030911
2211	20	0.08125372250015772
2211	21	0.12927085374499825
2211	22	0.021906735228043317
2211	23	0.2848909500986281
2211	24	0.2555354922286881
2211	25	0.23524868145698008
2211	26	0.12177767281325202
2211	27	0.1487577770219204
2211	28	0.1440279132549449
2211	29	0.0856323130290022
2211	30	0.27407904998047883
2211	31	0.28858375106562306
2211	32	0.16146523265007318
2211	33	0.11366183976169708
2211	34	0.03826159946825669
2211	35	0.09838974020736657
2211	36	0.16888350126026808
2211	37	0.19143037680752428
2211	38	0.12256174835640365
2211	39	0.027584293559229422
2211	40	0.003478341894478376
2211	41	0.03272375463892274
2211	42	0.07987756279185732
2211	43	-0.05005783379919393
2211	44	0.024573072293783582
2211	45	0.09397718187911863
2211	46	-0.10062825265297448
2211	47	-0.05089436515125101
2211	48	-0.027346557339704702
2211	49	-0.041876280517879394
2211	50	0.040120469298798866
2212	1	-0.18520175626796842
2212	2	-0.12143384624194908
2212	3	-0.3124403551121304
2212	4	-0.15417322582274906
2212	5	-0.15771007569153284
2212	6	-0.07274279984799392
2212	7	-0.10989545581608695
2212	8	-0.03651113063390454
2212	9	-0.21271064873766932
2212	10	-0.0011616336936626226
2212	11	-0.0622680092280703
2212	12	-0.08459878693705036
2212	13	0.008523288776061122
2212	14	-0.07342590815134287
2212	15	-0.0990170049732697
2212	16	-0.11259601691543963
2212	17	0.10876082679767052
2212	18	0.17454243347629728
2212	19	-0.008635216842144189
2212	20	-0.14029399130107834
2212	21	0.08201458888309787
2212	22	0.20491452809499577
2212	23	0.2755433823003557
2212	24	0.11508789262615693
2212	25	0.03250354561325253
2212	26	0.09736551600673238
2212	27	-0.08293244621759754
2212	28	-0.060325315041943384
2212	29	-0.11695849801250802
2212	30	0.1649123379968671
2212	31	-0.05553081347147781
2212	32	-0.09524332335426067
2212	33	-0.06203385776498749
2212	34	-0.18180350862502337
2212	35	-0.12259711458230527
2212	36	-0.052792912669666237
2212	37	-1.1214210149171038
2212	38	-0.601061563999302
2212	39	-0.3058160264608105
2212	40	-0.1665428499577004
2212	41	-1.0303501020969037
2212	42	-0.5895016939113447
2212	43	-0.3835312376951343
2212	44	-0.23453069033110197
2212	45	-0.2763322257542349
2212	46	-0.28206124042398883
2212	47	-0.09917421624800582
2212	48	-0.11101680157333149
2212	49	-0.20885406862242917
2212	50	-0.1057471195265336
2213	1	-0.9055179466398684
2213	2	-0.7752274039352294
2213	3	-0.7812906220125342
2213	4	-0.9653028538047472
2213	5	-0.8831090900877641
2213	6	-0.9004324255877592
2213	7	-0.9118690098787745
2213	8	-0.9790155947261141
2213	9	-1.1190982522004802
2213	10	-1.0698649823413007
2213	11	-1.066257827284737
2213	12	-1.0484607424424617
2213	13	-1.0461239949798617
2213	14	-2.844113744995166
2213	15	-1.7913671527551713
2213	16	-1.4973832478029436
2213	17	-1.209387178676245
2213	18	-1.2932454625845793
2213	19	-1.2440600657767433
2213	20	-1.4056280135988857
2213	21	-1.3187649936513566
2213	22	-1.4049743283119724
2213	23	-2.015716594892769
2213	24	-1.6358641591615495
2213	25	-1.4707785683931007
2213	26	-1.5402939210956035
2213	27	-1.4742717651335226
2213	28	-1.3976589768881382
2213	29	-1.6768136720738127
2213	30	-1.4141988065738857
2213	31	-1.3188003866296327
2213	32	-1.4032937950537558
2213	33	-1.3810367136759183
2213	34	-1.340835265846195
2213	35	-1.347892409515481
2213	36	-1.3109946534619397
2213	37	-1.5558370718958372
2213	38	-1.5210637931937327
2213	39	-1.420943082395663
2213	40	-1.5174836417062956
2213	41	-1.6914525006644823
2213	42	-1.4655700532666138
2213	43	-1.595444359079007
2213	44	-1.4765422547660663
2213	45	-1.4878413056477164
2213	46	-1.562365248443875
2213	47	-1.528030160424283
2213	48	-1.4806726419812577
2213	49	-1.5912264203541113
2213	50	-1.4926269903896916
2214	1	-0.6840713461621988
2214	2	-0.6925921094221117
2214	3	-0.7991153367030286
2214	4	-0.857825210370315
2214	5	-0.6758689445543524
2214	6	-0.6959542183273051
2214	7	-0.8044062285435167
2214	8	-0.747079620338102
2214	9	-1.0398517497348507
2214	10	-1.0766347852325175
2214	11	-0.9114945867495265
2214	12	-1.0457178681187074
2214	13	-1.1017004646819544
2214	14	-1.56417723650533
2214	15	-1.3181653850524313
2214	16	-0.9941478232814802
2214	17	-1.5777758983585315
2214	18	-1.6600175593746584
2214	19	-1.1164618634838135
2214	20	-1.263464331620419
2214	21	-1.2919232644251082
2214	22	-1.1393893164043902
2214	23	-0.8648482358972842
2214	24	-0.9914376253189943
2214	25	-1.0936208989507747
2214	26	-1.2197859049773072
2214	27	-1.1608191998962951
2214	28	-0.947997607094365
2214	29	-0.8759566969794221
2214	30	-0.8919391948030437
2214	31	-0.9227640384481504
2214	32	-1.0112432906839075
2214	33	-1.016300413224343
2214	34	-1.0530042592743456
2214	35	-0.9502792782050236
2214	36	-1.1057021432284877
2214	37	-1.1319338208933445
2214	38	-1.1264051071371384
2214	39	-1.0246637272209078
2214	40	-1.110533299325546
2214	41	-1.0262398037941907
2214	42	-0.9724872321569542
2214	43	-1.0515391503256524
2214	44	-1.0417686621173736
2214	45	-1.159778752239479
2214	46	-1.1500841589944244
2214	47	-1.025935460373347
2214	48	-1.1860287246493897
2214	49	-1.0532067584972147
2214	50	-1.1023064032387317
2215	1	-0.5880817094405337
2215	2	-0.7462991185102936
2215	3	-0.6172094621855528
2215	4	-0.6046774475413095
2215	5	-0.6494746839521177
2215	6	-0.6978153685928383
2215	7	-0.6571897574995589
2215	8	-0.7122584014799322
2215	9	-0.723653176688245
2215	10	-1.0940103845526572
2215	11	-0.7990191581957475
2215	12	-0.8357272346638922
2215	13	-0.9663881459012842
2215	14	-0.9262296828644097
2215	15	-1.0145757438677805
2215	16	-0.9659081472941367
2215	17	-0.9441319775581931
2215	18	-0.9457136375844541
2215	19	-1.1618431897282449
2215	20	-1.110595176305992
2215	21	-1.0773090794018003
2215	22	-1.520316538577184
2215	23	-1.351353215700854
2215	24	-1.141537656245184
2215	25	-0.9969708979065501
2215	26	-0.9976273454455296
2215	27	-1.0604416772015952
2215	28	-1.1436788687685748
2215	29	-1.1346645228509402
2215	30	-0.9486443423118232
2215	31	-1.0550679911359424
2215	32	-1.0822726150653352
2215	33	-1.1305014575054457
2215	34	-1.0775934430031064
2215	35	-1.0043150032497152
2215	36	-0.982490948805161
2215	37	-0.9033469190083352
2215	38	-0.8877099035006069
2215	39	-1.0600185730920302
2215	40	-0.9637751424350576
2215	41	-1.138040921199572
2215	42	-0.9418691574712028
2215	43	-1.0955174974136526
2215	44	-1.031766427764353
2215	45	-1.0610593283306429
2215	46	-1.0951734315537358
2215	47	-1.0621484294050987
2215	48	-1.1936897518395497
2215	49	-1.171908264668577
2215	50	-1.1909877601804908
2216	1	-1.0063130773792892
2216	2	-1.3098985347066368
2216	3	-1.0778596205313953
2216	4	-2.3787212003707268
2216	5	-1.4204203384017973
2216	6	-1.1978924249976188
2216	7	-1.1778882738089038
2216	8	-1.1797260238487972
2216	9	-1.0230647090917273
2216	10	-1.4904043729364034
2216	11	-1.31297675993682
2216	12	-1.5039114562497211
2216	13	-1.4310754008226318
2216	14	-1.3279934127188469
2216	15	-1.4987681547244165
2216	16	-1.5470602013796224
2216	17	-1.372953404916565
2216	18	-1.1914406825068156
2216	19	-1.447509405716339
2216	20	-1.4472775482846316
2216	21	-1.6498728930043853
2216	22	-1.8746703014898358
2216	23	-3.4510835943266223
2216	24	-2.2180564405181755
2216	25	-1.8004202873105832
2216	26	-1.9433167746755586
2216	27	-1.9564913197764717
2216	28	-2.3366908414074707
2216	29	-2.02636440741842
2216	30	-1.9107979877134582
2216	31	-1.5963706043112715
2216	32	-1.7996155277264663
2216	33	-1.8004575961887248
2216	34	-2.095052421873934
2216	35	-2.173753165738354
2216	36	-1.9888478487246068
2216	37	-1.878855022351111
2216	38	-2.121174334112073
2216	39	-2.21725368789847
2216	40	-2.1853168687317215
2216	41	-2.0984059201527216
2216	42	-2.2819511363607887
2216	43	-2.3218699260338234
2216	44	-2.474671180471592
2216	45	-2.348813798235806
2216	46	-2.3530150613778105
2216	47	-2.4479583150321034
2216	48	-2.432994373550901
2216	49	-2.6713532811216396
2216	50	-2.7594357732151877
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	195826.0
3	9732.0
4	18197.0
5	13748.0
6	11192.0
7	9465.0
8	8223.0
9	7385.0
10	7214.0
11	7074.0
12	7079.0
13	7253.0
14	7714.0
15	8272.0
16	8777.0
17	9566.0
18	10508.0
19	12172.0
20	15116.0
21	20335.0
22	25205.0
23	28968.0
24	31013.0
25	32641.0
26	35060.0
27	39273.0
28	45559.0
29	51062.0
30	58023.0
31	69166.0
32	82915.0
33	102681.0
34	129084.0
35	164805.0
36	213775.0
37	289479.0
38	455682.0
39	853056.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.64055441339355	17.954829732566367	12.992789864912375	24.41182598912771
2	15.575964655764968	20.225233376265344	39.27530383830212	24.923498129667564
3	18.646471032209373	25.43562964062006	29.29175509782798	26.62614422934259
4	12.569990364177322	16.485708691047524	37.80613576412848	33.13816518064667
5	13.76446931670919	37.824030268257204	34.330876467739806	14.080623947293796
6	31.61125057371759	36.95515374986332	17.767553984285218	13.666041692133868
7	27.86350381865008	32.38244936684776	21.95286816385527	17.80117865064689
8	26.440960043348056	34.58365384770447	20.235640553010256	18.739745555937215
9	26.976768337777163	15.839432514135382	19.18377141109989	38.00002773698756
10	14.916590838201033	28.88408131219964	33.48530207254438	22.714025777054957
11	34.503090515913776	23.505981877788763	23.58751408994667	18.40341351635079
12	23.494963238365642	25.13861618324787	30.08841307735507	21.278007501031414
13	27.91456348759639	22.341527358772648	25.93876591919341	23.80514323443755
14	22.439182365329327	21.29864709208502	27.173182728530413	29.08898781405524
15	23.657873844415793	28.934147870260357	23.976341997116624	23.431636288207226
16	23.556248602821228	28.250640242555075	25.222282473176588	22.970828681447113
17	22.459064416158594	27.86268407865981	26.636858769412825	23.041392735768767
18	22.5124747625771	27.41405889371783	27.551830465162112	22.521635878542956
19	23.285921878351637	27.096073918646262	27.647012336440156	21.97099186656194
20	23.670770549983594	26.526003573840057	27.51156787957211	22.29165799660424
21	23.942805489888404	26.366374949674302	27.298884317996468	22.391935242440823
22	23.614907737935866	26.17018723382841	27.1801957048672	23.034709323368517
23	23.50059047682334	26.274008987634662	27.072805905219845	23.152594630322156
24	22.99812218626287	26.445048951527117	27.321060993789864	23.235767868420155
25	23.31512497826514	26.30516420262986	27.33611432003843	23.04359649906657
26	23.05519035858489	26.542056979330837	27.483259639721314	22.91949302236296
27	23.123645679190375	26.670260445751193	27.167421392534553	23.03867248252388
28	23.131038185564563	26.510389341366285	27.23505227420711	23.123520198862042
29	23.178422277973944	26.626930661380577	27.246652315761217	22.947994744884266
30	23.423908765618325	26.568929676078202	27.097530101126367	22.909631457177106
31	23.580977462478863	26.259312138829017	27.029864005837446	23.129846392854677
32	23.102922650338495	26.65349358255733	27.06365503080082	23.179928736303353
33	23.286562026688518	26.447214125913156	27.075989528474903	23.190234318923427
34	23.48132955007649	26.427760684598756	27.141749886382954	22.949159878941803
35	23.473753860438045	26.546973864999764	27.036986972664888	22.942285301897307
36	23.508793778135395	26.49325492551914	26.909545055510193	23.088406240835276
37	23.576739980988997	26.280387112011972	27.073641497416478	23.069231409582553
38	23.38351984314014	26.479231004727023	27.055325812292715	23.081923339840127
39	23.470302344985424	26.71803636215861	26.800344844157344	23.011316448698626
40	23.546354763203382	26.333191521890516	27.120261156684656	23.000192558221446
41	23.30795397334216	26.559376059287526	26.995169568247707	23.137500399122608
42	23.61125535819687	26.448898364399316	26.889294572618283	23.050551704785526
43	23.37749446319784	26.309290863663744	26.89221906775343	23.420995605384984
44	23.34243543292157	26.627252898633913	26.798030128508575	23.232281539935943
45	23.451719684424887	26.482608057710323	26.7414273759636	23.32424488190119
46	23.55936357245587	26.334833895506698	26.612738869934798	23.493063662102635
47	23.44505367832344	26.586884077714668	26.656047618049588	23.312014625912305
48	23.386761280851786	26.78635120291892	26.575958907069424	23.250928609159867
49	23.53194818731722	26.585670111471316	26.433511314190177	23.448870387021287
50	23.689897962540044	26.632899260823383	26.469694790549426	23.20750798608715
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1943.0
1	1659.5
2	1376.0
3	1448.5
4	1521.0
5	1490.5
6	1460.0
7	1398.0
8	1336.0
9	1508.0
10	1680.0
11	2116.0
12	2552.0
13	4329.0
14	6106.0
15	8708.0
16	11310.0
17	12570.5
18	13831.0
19	14535.5
20	15240.0
21	17527.0
22	19814.0
23	24191.0
24	28568.0
25	35185.5
26	41803.0
27	49179.5
28	56556.0
29	64641.5
30	72727.0
31	80885.0
32	89043.0
33	99365.5
34	109688.0
35	119404.5
36	129121.0
37	137807.0
38	146493.0
39	155424.5
40	164356.0
41	173676.5
42	182997.0
43	187967.5
44	192938.0
45	199782.5
46	206627.0
47	212627.5
48	218628.0
49	217676.0
50	216724.0
51	214386.5
52	212049.0
53	205089.0
54	198129.0
55	189014.5
56	179900.0
57	167519.0
58	155138.0
59	141881.5
60	128625.0
61	112670.0
62	96715.0
63	83672.5
64	70630.0
65	59225.5
66	47821.0
67	39106.0
68	30391.0
69	24999.5
70	19608.0
71	15508.5
72	11409.0
73	9470.0
74	7531.0
75	5837.5
76	4144.0
77	3319.0
78	2494.0
79	1913.5
80	1333.0
81	1068.5
82	804.0
83	627.0
84	450.0
85	352.0
86	254.0
87	207.0
88	160.0
89	132.5
90	105.0
91	83.0
92	61.0
93	43.0
94	25.0
95	25.0
96	25.0
97	22.0
98	19.0
99	28.0
100	37.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06243764696780931
2	0.0658867064544152
3	0.06301786258237853
4	0.07749101874579949
5	0.08206827526073439
6	0.06130944993948029
7	0.04709416738253454
8	0.05834390346501541
9	0.05615197780997616
10	0.06756288489650404
11	0.054153457359793315
12	0.07052843137096891
13	0.06537095924146478
14	0.06717607448679123
15	0.07916719718788832
16	0.074074193460003
17	0.08603308196029069
18	0.07207567300982015
19	0.06865884772402367
20	0.0821327436623532
21	0.08016645741297974
22	0.07481558007861921
23	0.07278482542762696
24	0.060632531722482876
25	0.07852251317170031
26	0.08864405222585216
27	0.08039209681864555
28	0.0986688886775758
29	0.09151289609788882
30	0.07771665815146529
31	0.07442876966890641
32	0.08187487005587799
33	0.07929613399112592
34	0.06343690719290074
35	0.08322870648987282
36	0.08941767304527777
37	0.09789526785815017
38	0.07923166558950713
39	0.05686113022778298
40	0.06234094436538112
41	0.05621644621159497
42	0.061406152541908496
43	0.03955136439313476
44	0.038004122754283526
45	0.04158211904412701
46	0.03980923799960996
47	0.03062249076893074
48	0.04580479935015851
49	0.05115567668451905
50	0.0411630744336048
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3102295.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.077336362395016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.50696334159967	43.09736344351514
2	12.553760993149506	14.33070477638217
3	4.822818427001973	8.258208887182453
4	2.439273773971103	5.569089987076692
5	1.36480579602025	3.8949739744397043
6	0.8150129756269898	2.791126184974689
7	0.5306403134193066	2.120127495953633
8	0.3694547874378645	1.6869996138630514
9	0.2649369639356205	1.3609706584846617
>10	1.2475331121721875	12.357516467999563
>50	0.06569310465089512	2.528477774781607
>100	0.018765428867430014	1.6848040297878766
>500	1.1366071623698264E-4	0.03516198403659981
>1k	2.2732143100413687E-4	0.2844747215222047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3789	0.12213538686681956	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	6.446840161880156E-5	0.0	0.0	0.0	0.0
3	6.446840161880156E-5	0.0	0.0	0.0	0.0
4	6.446840161880156E-5	0.0	0.0	0.0	0.0
5	6.446840161880156E-5	0.0	0.0	0.0	0.0
6	6.446840161880156E-5	0.0	0.0	0.0	3.223420080940078E-5
7	6.446840161880156E-5	0.0	0.0	0.0	3.223420080940078E-5
8	6.446840161880156E-5	0.0	0.0	0.0	3.223420080940078E-5
9	6.446840161880156E-5	0.0	0.0	0.0	3.223420080940078E-5
10	9.670260242820234E-5	0.0	0.0	0.0	3.223420080940078E-5
11	9.670260242820234E-5	0.0	0.0	0.0	3.223420080940078E-5
12	1.611710040470039E-4	0.0	0.0	0.0	3.223420080940078E-5
13	1.611710040470039E-4	0.0	0.0	0.0	3.223420080940078E-5
14	1.611710040470039E-4	0.0	0.0	0.0	3.223420080940078E-5
15	2.2563940566580547E-4	0.0	0.0	0.0	6.446840161880156E-5
16	2.2563940566580547E-4	0.0	0.0	0.0	9.670260242820234E-5
17	2.2563940566580547E-4	0.0	0.0	0.0	9.670260242820234E-5
18	2.2563940566580547E-4	0.0	0.0	0.0	1.2893680323760313E-4
19	3.545762089034086E-4	0.0	0.0	6.446840161880156E-5	1.2893680323760313E-4
20	3.545762089034086E-4	0.0	0.0	6.446840161880156E-5	1.2893680323760313E-4
21	3.545762089034086E-4	3.223420080940078E-5	0.0	9.670260242820234E-5	1.2893680323760313E-4
22	3.545762089034086E-4	3.223420080940078E-5	0.0	9.670260242820234E-5	1.2893680323760313E-4
23	3.545762089034086E-4	3.223420080940078E-5	0.0	1.611710040470039E-4	1.2893680323760313E-4
24	4.5127881133161094E-4	3.223420080940078E-5	0.0	1.934052048564047E-4	1.2893680323760313E-4
25	4.8351301214101175E-4	3.223420080940078E-5	0.0	1.934052048564047E-4	1.611710040470039E-4
26	4.8351301214101175E-4	3.223420080940078E-5	0.0	3.223420080940078E-4	1.611710040470039E-4
27	5.157472129504125E-4	3.223420080940078E-5	0.0	4.8351301214101175E-4	1.611710040470039E-4
28	5.157472129504125E-4	3.223420080940078E-5	0.0	7.41386618616218E-4	1.611710040470039E-4
29	5.802156145692141E-4	3.223420080940078E-5	0.0	0.0013538364339948329	1.611710040470039E-4
30	5.802156145692141E-4	3.223420080940078E-5	0.0	0.002546501863942662	1.611710040470039E-4
31	5.802156145692141E-4	3.223420080940078E-5	0.0	0.004802895920600717	1.611710040470039E-4
32	5.802156145692141E-4	3.223420080940078E-5	0.0	0.007091524178068172	1.934052048564047E-4
33	6.124498153786148E-4	3.223420080940078E-5	0.0	0.009992602250914242	1.934052048564047E-4
34	6.124498153786148E-4	3.223420080940078E-5	0.0	0.014279750958564546	2.2563940566580547E-4
35	7.091524178068172E-4	3.223420080940078E-5	0.0	0.019211583682402868	2.2563940566580547E-4
36	8.058550202350196E-4	3.223420080940078E-5	0.0	0.024917037225666804	2.2563940566580547E-4
37	8.058550202350196E-4	3.223420080940078E-5	0.0	0.033136758432064005	2.2563940566580547E-4
38	9.347918234726227E-4	3.223420080940078E-5	0.0	0.04171105584736461	2.2563940566580547E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2550	0.0	25.451529	1
GTATCAA	5690	0.0	24.397715	1
TATCAAC	7465	0.0	18.536976	2
TCAACGC	7425	0.0	18.370768	4
ATCAACG	7470	0.0	18.23006	3
CAACGCA	7565	0.0	18.05871	5
AACGCAG	7775	0.0	17.5701	6
ACCGTCG	110	5.626306E-5	15.99983	8
CGTACAC	265	0.0	15.773417	3
CGTGCCG	140	1.5855912E-6	15.716655	10
TCGATCG	100	4.960553E-4	15.394373	41
GTACATG	9700	0.0	14.992077	1
ACGCAGA	9105	0.0	14.907892	7
CGCGCGT	150	3.2486532E-6	14.669115	32
CGCAGAG	9275	0.0	14.563726	8
TACATGG	9745	0.0	14.538584	2
AGAGTAC	9000	0.0	14.179686	11
ACATGGG	10075	0.0	13.90953	3
GAGTACT	4480	0.0	13.899191	12
CAGAGTA	9605	0.0	13.722006	10
>>END_MODULE