FastQCFastQC Report
Mon 6 Feb 2017
SRR2077416_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077416_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3102295
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41170.13270820473230302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28100.026.9377441
GTATCAA60600.023.5294361
TATCAAC84300.016.9394072
ATCAACG86200.016.7699663
TCAACGC87550.016.4870434
CAACGCA89100.016.2254525
TGCGATA1501.8374885E-716.13550213
AACGCAG91500.015.7737826
ACCGTGC4350.015.6792588
GCGATAC1552.6670932E-715.61500314
GTACATG89700.014.3752131
TACATGG91650.013.8283742
GTACCGA2805.820766E-1113.3580786
CGAATCG1658.741952E-613.33082837
ACATGGG94300.012.9962363
ACGCAGA110500.012.9819047
CGCAGAG113200.012.6528288
TACCGAT2452.2158929E-812.5723097
GAGTACT68350.012.55472412
GCACCGT3357.2759576E-1212.4784856
Produced by FastQC (version 0.11.5)