FastQCFastQC Report
Mon 6 Feb 2017
SRR2077415_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077415_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3480269
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38750.11134196810648832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57900.025.0811481
GGTATCA24800.023.6887191
CGTGCCG2800.018.85890610
TATCAAC75250.018.8583642
TTAGTCG1203.1765012E-718.32766739
TCAACGC76650.018.3122024
ATCAACG77000.018.2287033
CAACGCA77400.018.2184585
TATACCG1002.4865692E-517.598865
AACGCAG81200.017.3643726
GTACATG93700.015.7801721
TACATGG93400.015.4528212
GCACCGT4500.015.1532676
ACGCAGA93700.015.0254937
GAGTACT49250.014.96608112
AGAGTAC89600.014.61095111
ACATGGG97050.014.5760973
AGTACTT51600.014.41240813
CGCAGAG96100.014.3297728
TGCGTCG1100.001017954814.00130810
Produced by FastQC (version 0.11.5)