##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077415_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3480269 Sequences flagged as poor quality 0 Sequence length 50 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.363696886648704 31.0 30.0 34.0 23.0 34.0 2 29.34018261232106 31.0 30.0 34.0 23.0 34.0 3 29.408330792820898 31.0 30.0 34.0 23.0 34.0 4 32.78240360156068 37.0 35.0 37.0 25.0 37.0 5 32.82950312174145 37.0 35.0 37.0 25.0 37.0 6 32.87317675731387 37.0 35.0 37.0 25.0 37.0 7 32.692308841644135 37.0 35.0 37.0 25.0 37.0 8 32.620469279817165 37.0 33.0 37.0 23.0 37.0 9 33.859903932713245 38.0 34.0 39.0 22.0 39.0 10 33.76157934918249 38.0 34.0 39.0 21.0 39.0 11 33.85007739344287 39.0 34.0 39.0 22.0 39.0 12 33.77269027193013 38.0 34.0 39.0 21.0 39.0 13 33.79437394063505 38.0 34.0 39.0 22.0 39.0 14 34.68191338083349 39.0 34.0 41.0 18.0 41.0 15 34.78741671979953 39.0 34.0 41.0 19.0 41.0 16 34.69545342615758 39.0 34.0 41.0 18.0 41.0 17 34.52740262318804 39.0 34.0 41.0 17.0 41.0 18 34.35193170412977 39.0 33.0 41.0 17.0 41.0 19 34.69912699276981 39.0 34.0 41.0 18.0 41.0 20 34.73619366778832 39.0 34.0 41.0 19.0 41.0 21 34.732760025158974 39.0 34.0 41.0 19.0 41.0 22 34.64536620588811 39.0 34.0 41.0 19.0 41.0 23 34.39241190838984 39.0 34.0 41.0 18.0 41.0 24 34.54109438092286 39.0 34.0 41.0 18.0 41.0 25 34.449027072332626 39.0 34.0 41.0 18.0 41.0 26 34.49157464552309 39.0 34.0 41.0 18.0 41.0 27 34.47409266352687 39.0 34.0 41.0 18.0 41.0 28 34.36208379294819 39.0 34.0 41.0 18.0 41.0 29 34.32473208249132 39.0 34.0 41.0 17.0 41.0 30 34.100685607922834 39.0 34.0 41.0 16.0 41.0 31 34.018217557321 39.0 33.0 41.0 16.0 41.0 32 34.0278579040873 39.0 33.0 41.0 16.0 41.0 33 34.016246732651986 39.0 33.0 41.0 16.0 41.0 34 33.99461018674131 39.0 33.0 41.0 16.0 41.0 35 33.932738819901566 39.0 33.0 41.0 15.0 41.0 36 33.80617475258378 39.0 33.0 41.0 10.0 41.0 37 33.712911559422565 39.0 33.0 41.0 9.0 41.0 38 33.616249491059456 38.0 33.0 41.0 9.0 41.0 39 33.55103729050829 38.0 33.0 41.0 9.0 41.0 40 33.5071421203361 38.0 33.0 41.0 8.0 41.0 41 33.38754504321361 38.0 33.0 41.0 7.0 41.0 42 33.20287914526147 38.0 33.0 40.0 2.0 41.0 43 33.099837110292334 38.0 33.0 40.0 2.0 41.0 44 33.032825623536574 38.0 33.0 40.0 2.0 41.0 45 32.94248461828669 38.0 33.0 40.0 2.0 41.0 46 32.77672386818375 38.0 32.0 40.0 2.0 41.0 47 32.594662653950024 38.0 32.0 40.0 2.0 41.0 48 32.40580340197841 38.0 32.0 40.0 2.0 41.0 49 32.270315024499546 38.0 32.0 40.0 2.0 41.0 50 32.07130224703895 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0146224240210664 1101 2 0.9887730682743232 1101 3 1.1061395329533887 1101 4 1.0689799800427338 1101 5 0.9096730636906258 1101 6 0.9606242161400687 1101 7 0.9691751197139666 1101 8 0.9815476361420608 1101 9 1.2166595457821145 1101 10 1.2487994547087027 1101 11 1.2192301397894028 1101 12 1.2157860765220505 1101 13 1.1791393681935673 1101 14 1.4870414810284558 1101 15 1.477049625611599 1101 16 1.3706344976252467 1101 17 1.5227473496376547 1101 18 1.6546846418186476 1101 19 1.302209631795769 1101 20 1.3744730295001233 1101 21 1.3827737859729368 1101 22 1.2927212009802034 1101 23 1.4209364369492476 1101 24 1.2500568049235454 1101 25 1.29866029999922 1101 26 1.2482372232369414 1101 27 1.334190101138752 1101 28 1.430030199459189 1101 29 1.3676112137937793 1101 30 1.5829447440764781 1101 31 1.6196308746745984 1101 32 1.6423247481049117 1101 33 1.5455089230279242 1101 34 1.47141326046102 1101 35 1.421572161541178 1101 36 1.537558360309447 1101 37 1.5729534130255942 1101 38 1.5595682014648062 1101 39 1.5671345071893938 1101 40 1.4832795318951568 1101 41 1.484667211822142 1101 42 1.5242837727415761 1101 43 1.5891118352116393 1101 44 1.5026882745005068 1101 45 1.5326119226585533 1101 46 1.4607661481046819 1101 47 1.566853945556879 1101 48 1.5359412980516822 1101 49 1.6893238370436592 1101 50 1.630776672828624 1102 1 0.5548566425998871 1102 2 0.6127778282002794 1102 3 0.5758308933100054 1102 4 0.5963189547697922 1102 5 0.5465462456633503 1102 6 0.5908618346660077 1102 7 0.5899287746571176 1102 8 0.5589723650907246 1102 9 0.825770648498299 1102 10 0.8889418747154991 1102 11 0.713161234194601 1102 12 0.6113162793855551 1102 13 0.7878670324299009 1102 14 1.090119188708563 1102 15 0.9993948335750247 1102 16 1.0441198211868752 1102 17 1.0384422610501431 1102 18 1.166807342132195 1102 19 0.9580870070365961 1102 20 0.8719812460389775 1102 21 0.8332203123603037 1102 22 0.9587659291733175 1102 23 1.2291567535220977 1102 24 0.99149422700809 1102 25 0.980856665833528 1102 26 0.8777302533453621 1102 27 1.0245214070962163 1102 28 0.996010328657178 1102 29 0.8071310657071891 1102 30 1.0899701681254328 1102 31 1.123831320445447 1102 32 1.0067314573338777 1102 33 0.8661129802981478 1102 34 0.7998020633018612 1102 35 0.8729079629842715 1102 36 0.9153257283726077 1102 37 0.9005972496770198 1102 38 1.1006342737703463 1102 39 0.9938652691553216 1102 40 0.9858419587018545 1102 41 0.9957449950566257 1102 42 0.9951998696022955 1102 43 0.965403248456326 1102 44 0.930220668441045 1102 45 0.9218850895204014 1102 46 0.9750776588145129 1102 47 1.0144762492099346 1102 48 0.9915036115268023 1102 49 1.0966703672087448 1102 50 1.0926674211947613 1103 1 0.5928560914426058 1103 2 0.5798876672563651 1103 3 0.7123784620773606 1103 4 0.6045452767235204 1103 5 0.5565880916807942 1103 6 0.6167561921933498 1103 7 0.6955609131174114 1103 8 0.7487858402871481 1103 9 0.8533371992422119 1103 10 0.9001606212520485 1103 11 0.8965383006599765 1103 12 0.7455943681877812 1103 13 0.8199482802020555 1103 14 1.0812737134402468 1103 15 1.037070777057444 1103 16 1.1646516280541803 1103 17 1.348942406328014 1103 18 0.9742710301532114 1103 19 0.9461733230593978 1103 20 1.0104460333791323 1103 21 1.0161537571232628 1103 22 1.072058854290809 1103 23 1.2504281295880944 1103 24 1.170428935838288 1103 25 0.8799823027347102 1103 26 0.9666873379629806 1103 27 1.0040912947380107 1103 28 1.1553123059339256 1103 29 1.096863065479539 1103 30 1.2360030338737076 1103 31 1.2327341580068136 1103 32 1.1426264738536176 1103 33 1.1703814538021575 1103 34 1.0496224878726963 1103 35 1.0172642921569661 1103 36 1.0831450326563612 1103 37 1.150087578963543 1103 38 1.0486817092415137 1103 39 1.1157445657108909 1103 40 1.183474678273626 1103 41 1.3356827801831699 1103 42 1.22260167224605 1103 43 1.2267495998849895 1103 44 1.2089284742060329 1103 45 1.062562893158642 1103 46 1.094372506991654 1103 47 1.0610452951387117 1103 48 1.1400376529774263 1103 49 1.1641311197181636 1103 50 1.273820388308046 1104 1 0.7555263473196518 1104 2 0.7207968989794686 1104 3 0.7755983712431984 1104 4 0.7944911607742 1104 5 0.6754670139344938 1104 6 0.7436536064870936 1104 7 0.7423783795607335 1104 8 0.801069187779234 1104 9 1.0347430009408782 1104 10 1.0706872016688749 1104 11 0.9055095085838687 1104 12 0.9260900684219564 1104 13 0.8581492346777821 1104 14 1.1148484581864153 1104 15 1.1500484021602162 1104 16 1.1871434240512428 1104 17 1.2330047880202741 1104 18 1.3500478161155272 1104 19 1.1886636588217527 1104 20 1.066132025903137 1104 21 1.0501607909749637 1104 22 1.0026362564023614 1104 23 1.2601562161410342 1104 24 1.1495026016251728 1104 25 1.1956757389651358 1104 26 1.102271476207065 1104 27 1.0574601463899427 1104 28 1.2402755317262049 1104 29 1.248777252837833 1104 30 1.386140905337271 1104 31 1.4289561788407568 1104 32 1.2257704420879847 1104 33 1.2176344817893607 1104 34 1.1133301721542423 1104 35 1.128112105302769 1104 36 1.2229433806422207 1104 37 1.174469135384669 1104 38 1.2059429517761728 1104 39 1.1479082714026205 1104 40 1.1244200013556522 1104 41 1.2543934118408515 1104 42 1.20832402374171 1104 43 1.1898918359086608 1104 44 1.0560821873254653 1104 45 1.089657773654281 1104 46 1.1568506634656899 1104 47 1.2147975383951959 1104 48 1.1650474448401695 1104 49 1.0285294557487106 1104 50 1.15679333891444 1105 1 0.7610827662016106 1105 2 0.840883917982179 1105 3 0.7478443976311837 1105 4 0.7539104136407815 1105 5 0.7628491868962399 1105 6 0.6518595490242305 1105 7 0.7329682650578633 1105 8 0.8211489777666685 1105 9 1.0586570348381557 1105 10 1.0915910205878419 1105 11 1.0614765878345835 1105 12 1.0998753807107562 1105 13 1.0423804740397955 1105 14 1.2997906701261002 1105 15 1.1517947574361571 1105 16 1.16133214978219 1105 17 1.1901097171411763 1105 18 1.4180800860385077 1105 19 1.1907950720025298 1105 20 1.202538314043089 1105 21 1.1645220215009502 1105 22 1.244669097052153 1105 23 1.5077149547559614 1105 24 1.300292767384562 1105 25 1.3707369831968848 1105 26 1.2990207334776898 1105 27 1.177201834831422 1105 28 1.2853753806190085 1105 29 1.1987759309812276 1105 30 1.4295180419178735 1105 31 1.4042877552869513 1105 32 1.289570478211573 1105 33 1.2930970545837184 1105 34 1.232317936633926 1105 35 1.1889330563503009 1105 36 1.1112821049295079 1105 37 1.1786971421087316 1105 38 1.220397328414748 1105 39 1.2693886132454821 1105 40 1.3632342465422127 1105 41 1.402404490503784 1105 42 1.4952529897467812 1105 43 1.324130888195505 1105 44 1.3239994879638743 1105 45 1.28871458418552 1105 46 1.2979374703265378 1105 47 1.3546705656495845 1105 48 1.4809698130970972 1105 49 1.4109699412147023 1105 50 1.4009191191949384 1106 1 0.46015791618723867 1106 2 0.43460708910503953 1106 3 0.43865387933915656 1106 4 0.49868281726702435 1106 5 0.4110312307042321 1106 6 0.4355019873008743 1106 7 0.4456719244446816 1106 8 0.5336143716495272 1106 9 -0.825366777627579 1106 10 -0.35540424120437564 1106 11 0.2935904029870713 1106 12 0.5045767549681415 1106 13 0.6185560790239677 1106 14 0.7350378313734822 1106 15 0.6971464033887642 1106 16 0.745248342638746 1106 17 0.8736833113053493 1106 18 -0.9258111940415787 1106 19 0.12254432919675651 1106 20 0.3270936602493748 1106 21 0.5149362065465013 1106 22 0.6229772049722371 1106 23 -0.7565854036132791 1106 24 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0.5652522666803463 1107 12 0.649912967468083 1107 13 0.5479106483922536 1107 14 0.8200764776416847 1107 15 0.7263104068263146 1107 16 0.6124805125270285 1107 17 0.8892373022228881 1107 18 0.9266864480954595 1107 19 0.7757503859163322 1107 20 0.8264125192269276 1107 21 0.624049920540557 1107 22 0.7892657934389931 1107 23 0.9488099975724111 1107 24 0.8491753418393273 1107 25 0.7661003541875218 1107 26 0.742587189369253 1107 27 0.7682709320260841 1107 28 0.7719647219247534 1107 29 0.707244539600957 1107 30 0.9025744573300329 1107 31 0.9455319810191511 1107 32 0.8622994602295435 1107 33 0.7805118582277473 1107 34 0.7031034839877748 1107 35 0.6435283593284922 1107 36 0.7655032688666807 1107 37 0.7622539323301751 1107 38 0.7168163387418431 1107 39 0.7205461125179156 1107 40 0.7874944788357752 1107 41 0.7101284897629867 1107 42 0.8212239398221826 1107 43 0.8094649623283701 1107 44 0.6792238355752431 1107 45 0.6968088068310507 1107 46 0.7856728990642665 1107 47 0.8263852167241836 1107 48 0.6963540323032049 1107 49 0.8341375647474294 1107 50 0.8450715475181738 1108 1 0.17514819171992002 1108 2 0.25629771587183114 1108 3 0.22632103947180937 1108 4 0.24015279414527413 1108 5 0.35202892668755936 1108 6 0.23072566940894035 1108 7 0.23434347488272067 1108 8 0.2847946417497269 1108 9 0.32485389231212736 1108 10 0.2740179818291111 1108 11 0.3152049321412207 1108 12 0.2954586347547945 1108 13 0.350510293511789 1108 14 0.22253430772766336 1108 15 0.27675832768969144 1108 16 0.37842625181846756 1108 17 0.23846004543172228 1108 18 0.6219580002260301 1108 19 0.37136039457404735 1108 20 0.27294231975667316 1108 21 0.4314752199173739 1108 22 0.31587466202178405 1108 23 0.19705752240975727 1108 24 0.34543490175326497 1108 25 0.31401134262390684 1108 26 0.31023515480698194 1108 27 0.3317646701860184 1108 28 0.41701784255840124 1108 29 0.31548362959829745 1108 30 0.46642156728297124 1108 31 0.628865662946815 1108 32 0.48390355421285847 1108 33 0.4942789229205644 1108 34 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-3.741185095795526 2216 24 -2.4992576583026036 2216 25 -2.109905566211495 2216 26 -2.305251743336896 2216 27 -2.0905213876754587 2216 28 -2.4950029389093693 2216 29 -2.222314552874863 2216 30 -2.1084196425462878 2216 31 -1.9035656447386415 2216 32 -1.8505180414431734 2216 33 -2.0067899401948353 2216 34 -2.2490277380069124 2216 35 -2.4316383242342923 2216 36 -2.311067603581158 2216 37 -2.3284808054083115 2216 38 -2.3545830246125092 2216 39 -2.417289697786014 2216 40 -2.4722559722905153 2216 41 -2.4211703890107614 2216 42 -2.7047068401701146 2216 43 -2.639757397315595 2216 44 -2.692031086263327 2216 45 -2.6666118861800356 2216 46 -2.562569188484648 2216 47 -2.551550672653562 2216 48 -2.7012046546658333 2216 49 -2.9785101917862953 2216 50 -3.004694840024662 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 168775.0 3 8432.0 4 16148.0 5 12391.0 6 10181.0 7 8717.0 8 7712.0 9 7004.0 10 6838.0 11 6530.0 12 6765.0 13 7198.0 14 7570.0 15 8070.0 16 8750.0 17 9476.0 18 10579.0 19 12161.0 20 15225.0 21 20465.0 22 25791.0 23 29524.0 24 31797.0 25 33343.0 26 35914.0 27 41216.0 28 47298.0 29 53577.0 30 62256.0 31 74430.0 32 90126.0 33 111351.0 34 141284.0 35 181349.0 36 239005.0 37 329186.0 38 526789.0 39 1067046.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.137739815177675 17.462751323086 12.807403473735086 24.59210538800124 2 15.753732469791615 19.641366619464193 39.392655103258406 25.212245807485782 3 18.75838119575943 25.014002203550064 28.842095396696514 27.38552120399399 4 12.684515838319365 16.171945339631645 37.52714197680147 33.61639684524753 5 14.041299360258497 37.56857967953684 34.179583141376376 14.21053781882829 6 32.440101308738925 36.68798972766879 17.39522455054957 13.476684413042708 7 28.571420358031034 31.963582288120318 21.543437846489873 17.921559507358772 8 26.70450216562231 34.69213375251386 19.701772317122433 18.901591764741397 9 27.045273159254158 15.746245828378754 18.735179388338548 38.47330162402854 10 14.77174479028146 28.726087710334557 33.451515265653455 23.050652233730528 11 35.17664507890071 23.08602993538045 23.312228553751925 18.425096431966914 12 23.75890328084695 24.726874041597092 29.92807790922456 21.58614476833139 13 28.215403875077666 21.908073361205922 25.667595082072324 24.208927681644084 14 22.592745623546346 20.856655777827424 26.787694662801087 29.76290393582514 15 23.835711453192797 28.73798658621347 23.523156366401178 23.903145594192555 16 23.897498185549036 27.972076433531285 24.87180947057139 23.258615910348293 17 22.842545144060388 27.623238661968326 26.240539597734653 23.293676596236633 18 22.891349397791295 27.305227097393427 27.00595580917832 22.797467695636957 19 23.658315673854098 26.893666452931857 27.128238010483546 22.319779862730496 20 23.895043393064757 26.407524704527724 27.02220826543057 22.675223636976945 21 24.13951364389789 26.171489929983217 26.948035515659168 22.74096091045973 22 23.860617719538883 25.921619033652227 26.915226749251648 23.302536497557245 23 23.714285303509325 26.05609685614344 26.803393588068097 23.42622425227914 24 23.277169247259025 26.18639492004639 26.9482672070318 23.58816862566278 25 23.62772531875363 25.998920497859256 26.955893106858348 23.417461076528763 26 23.33983001640132 26.39922419423443 27.023124338001224 23.237821451363025 27 23.478024066811486 26.35904213561176 26.779086961097757 23.383846836478998 28 23.549790497490804 26.201619416430244 26.873126154794857 23.375463931284095 29 23.596231404141037 26.300131922137464 26.83457907289827 23.269057600823224 30 23.65114730263968 26.35161326313828 26.79379263780112 23.203446796420927 31 23.849638762086194 25.983767657524893 26.68203155889436 23.484562021494554 32 23.403522323008918 26.342855811821465 26.788329627042568 23.46529223812705 33 23.621692657635247 26.176753923088203 26.689968914360644 23.511584504915902 34 23.780495168214006 26.129194568158226 26.732585587735514 23.35772467589225 35 23.75577276631796 26.272194181781998 26.680526002935018 23.29150704896502 36 23.769689129676404 26.293538684004442 26.606528584522373 23.33024360179678 37 23.825485114302815 25.980158539556225 26.736242835132035 23.458113511008925 38 23.726703144895716 26.13825762586312 26.77894630292727 23.356092926313902 39 23.827100900070523 26.37729592445468 26.537518655037555 23.25808452043724 40 23.827232124630612 26.052585727536144 26.847628012676054 23.27255413515719 41 23.622901365131487 26.124487640849352 26.794877802863663 23.457733191155498 42 23.79502354304667 26.196164166784307 26.61984412740645 23.38896816276257 43 23.59761820238381 26.00962664761842 26.727557823842986 23.66519732615479 44 23.67982242698641 26.27169970338385 26.523416693806418 23.525061175823325 45 23.730441829038785 26.32735127080142 26.374666586562164 23.567540313597636 46 23.793746938662878 26.157875311307805 26.336956346769874 23.711421403259447 47 23.745618971684916 26.315918057165916 26.311520502091568 23.626942469057596 48 23.62157937146072 26.500666784145626 26.340632839816287 23.53712100457737 49 23.844696534118352 26.21328856282054 26.311061153147353 23.630953749913754 50 23.91411144171805 26.323658217648855 26.186139019146616 23.576091321486484 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1944.0 1 1658.0 2 1372.0 3 1378.0 4 1384.0 5 1382.5 6 1381.0 7 1334.0 8 1287.0 9 1419.5 10 1552.0 11 1988.5 12 2425.0 13 4405.5 14 6386.0 15 9197.5 16 12009.0 17 13338.5 18 14668.0 19 15541.5 20 16415.0 21 18970.5 22 21526.0 23 26007.0 24 30488.0 25 37486.0 26 44484.0 27 52023.0 28 59562.0 29 67708.0 30 75854.0 31 84122.5 32 92391.0 33 103282.5 34 114174.0 35 125015.0 36 135856.0 37 144940.5 38 154025.0 39 164679.5 40 175334.0 41 185755.0 42 196176.0 43 203131.5 44 210087.0 45 219341.0 46 228595.0 47 234942.5 48 241290.0 49 242542.5 50 243795.0 51 243429.0 52 243063.0 53 237725.5 54 232388.0 55 223377.5 56 214367.0 57 201513.5 58 188660.0 59 172126.5 60 155593.0 61 137492.5 62 119392.0 63 102619.5 64 85847.0 65 72584.0 66 59321.0 67 48804.5 68 38288.0 69 31189.5 70 24091.0 71 19010.5 72 13930.0 73 11470.5 74 9011.0 75 7031.0 76 5051.0 77 3931.5 78 2812.0 79 2283.0 80 1754.0 81 1350.5 82 947.0 83 751.0 84 555.0 85 434.0 86 313.0 87 238.5 88 164.0 89 134.0 90 104.0 91 78.5 92 53.0 93 46.5 94 40.0 95 29.5 96 19.0 97 16.0 98 13.0 99 23.0 100 33.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06396057316259174 2 0.06735111567525384 3 0.06450650797395259 4 0.07821234508022225 5 0.08410269436069454 6 0.06286870353987005 7 0.04689292695478424 8 0.059018426449219875 9 0.05761048930413138 10 0.06602937876353811 11 0.054880815247327144 12 0.07120139276590401 13 0.0660868455857866 14 0.0688452530537151 15 0.08051101797016266 16 0.07565507149016354 17 0.08654503430625621 18 0.07148872687714657 19 0.07008078973205807 20 0.082464889926612 21 0.08094201913702648 22 0.07582747195690909 23 0.07281046378886229 24 0.06111596546129049 25 0.07872954648045884 26 0.08936090859643321 27 0.0799650831588018 28 0.09944633590104673 29 0.09246411699785274 30 0.078183611669098 31 0.07597113901253036 32 0.08183275488187838 33 0.07985014951430479 34 0.06490877572969216 35 0.0847635628165524 36 0.09079757915264595 37 0.09792346511146122 38 0.0793329481140682 39 0.057064554492770536 40 0.06364450564022493 41 0.05735188860401308 42 0.06240896896188197 43 0.039767040995968984 44 0.03913490595123538 45 0.042525448463897476 46 0.04014057534058431 47 0.03054361602508312 48 0.04689292695478424 49 0.05140407250129229 50 0.04258291528614598 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3480269.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.01932439973783 #Duplication Level Percentage of deduplicated Percentage of total 1 79.78820817412803 50.28198974199187 2 11.481556530261008 14.471198711888933 3 3.809062891706102 7.201337100942908 4 1.6895990065333006 4.259095515927873 5 0.887293092952326 2.795830563120468 6 0.5406939484002854 2.0444500401127614 7 0.3483351310017186 1.5366291234295733 8 0.25146784714652076 1.2677867068344224 9 0.18550669900680108 1.0521456158730709 >10 0.9470185576908643 10.603180538943269 >50 0.05195382936366449 2.2064326898172184 >100 0.018661722217141033 1.8465478516851386 >500 5.048761086834844E-4 0.20759114467137543 >1k 1.3769348340261106E-4 0.22578465476122117 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3875 0.11134196810648832 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.873341112425505E-5 0.0 0.0 0.0 2.873341112425505E-5 2 2.873341112425505E-5 2.873341112425505E-5 0.0 0.0 2.873341112425505E-5 3 2.873341112425505E-5 2.873341112425505E-5 0.0 0.0 2.873341112425505E-5 4 2.873341112425505E-5 2.873341112425505E-5 0.0 0.0 2.873341112425505E-5 5 2.873341112425505E-5 2.873341112425505E-5 0.0 0.0 2.873341112425505E-5 6 2.873341112425505E-5 2.873341112425505E-5 0.0 0.0 2.873341112425505E-5 7 2.873341112425505E-5 2.873341112425505E-5 0.0 0.0 2.873341112425505E-5 8 2.873341112425505E-5 2.873341112425505E-5 0.0 0.0 5.74668222485101E-5 9 2.873341112425505E-5 2.873341112425505E-5 0.0 0.0 8.620023337276514E-5 10 2.873341112425505E-5 2.873341112425505E-5 0.0 0.0 8.620023337276514E-5 11 2.873341112425505E-5 2.873341112425505E-5 0.0 0.0 8.620023337276514E-5 12 2.873341112425505E-5 2.873341112425505E-5 0.0 0.0 1.149336444970202E-4 13 2.873341112425505E-5 2.873341112425505E-5 0.0 0.0 1.149336444970202E-4 14 5.74668222485101E-5 2.873341112425505E-5 0.0 0.0 1.149336444970202E-4 15 5.74668222485101E-5 2.873341112425505E-5 0.0 0.0 1.724004667455303E-4 16 5.74668222485101E-5 2.873341112425505E-5 0.0 0.0 1.724004667455303E-4 17 5.74668222485101E-5 2.873341112425505E-5 0.0 0.0 1.724004667455303E-4 18 5.74668222485101E-5 2.873341112425505E-5 0.0 0.0 1.724004667455303E-4 19 8.620023337276514E-5 2.873341112425505E-5 0.0 0.0 1.724004667455303E-4 20 8.620023337276514E-5 2.873341112425505E-5 0.0 0.0 2.298672889940404E-4 21 8.620023337276514E-5 2.873341112425505E-5 0.0 0.0 2.298672889940404E-4 22 1.149336444970202E-4 2.873341112425505E-5 0.0 2.873341112425505E-5 2.298672889940404E-4 23 1.149336444970202E-4 2.873341112425505E-5 0.0 2.873341112425505E-5 2.298672889940404E-4 24 1.724004667455303E-4 2.873341112425505E-5 0.0 2.873341112425505E-5 2.298672889940404E-4 25 1.724004667455303E-4 2.873341112425505E-5 0.0 5.74668222485101E-5 2.298672889940404E-4 26 1.724004667455303E-4 2.873341112425505E-5 0.0 1.4366705562127526E-4 2.8733411124255053E-4 27 1.724004667455303E-4 2.873341112425505E-5 0.0 1.724004667455303E-4 2.8733411124255053E-4 28 1.724004667455303E-4 2.873341112425505E-5 0.0 3.448009334910606E-4 2.8733411124255053E-4 29 2.0113387786978534E-4 2.873341112425505E-5 0.0 6.896018669821212E-4 2.8733411124255053E-4 30 2.0113387786978534E-4 2.873341112425505E-5 0.0 0.0012642700894672223 2.8733411124255053E-4 31 2.0113387786978534E-4 2.873341112425505E-5 0.0 0.0024710733566859345 2.8733411124255053E-4 32 2.0113387786978534E-4 2.873341112425505E-5 0.0 0.004108877790768472 2.8733411124255053E-4 33 2.0113387786978534E-4 2.873341112425505E-5 0.0 0.006120216569466326 2.8733411124255053E-4 34 2.586007001182955E-4 2.873341112425505E-5 0.0 0.00847635628165524 2.8733411124255053E-4 35 3.160675223668056E-4 2.873341112425505E-5 0.0 0.01149336444970202 2.8733411124255053E-4 36 3.448009334910606E-4 2.873341112425505E-5 0.0 0.014452905795500291 2.8733411124255053E-4 37 3.448009334910606E-4 2.873341112425505E-5 0.0 0.01882038428638706 2.8733411124255053E-4 38 3.448009334910606E-4 2.873341112425505E-5 0.0 0.024710733566859343 2.8733411124255053E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5790 0.0 25.081148 1 GGTATCA 2480 0.0 23.688719 1 CGTGCCG 280 0.0 18.858906 10 TATCAAC 7525 0.0 18.858364 2 TTAGTCG 120 3.1765012E-7 18.327667 39 TCAACGC 7665 0.0 18.312202 4 ATCAACG 7700 0.0 18.228703 3 CAACGCA 7740 0.0 18.218458 5 TATACCG 100 2.4865692E-5 17.59886 5 AACGCAG 8120 0.0 17.364372 6 GTACATG 9370 0.0 15.780172 1 TACATGG 9340 0.0 15.452821 2 GCACCGT 450 0.0 15.153267 6 ACGCAGA 9370 0.0 15.025493 7 GAGTACT 4925 0.0 14.966081 12 AGAGTAC 8960 0.0 14.610951 11 ACATGGG 9705 0.0 14.576097 3 AGTACTT 5160 0.0 14.412408 13 CGCAGAG 9610 0.0 14.329772 8 TGCGTCG 110 0.0010179548 14.001308 10 >>END_MODULE