FastQCFastQC Report
Mon 6 Feb 2017
SRR2077415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3480269
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42820.12303646643406013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26850.025.2395821
GTATCAA60300.023.90011
TAACGCG600.00740776518.33636512
TACCGAT3550.017.3527367
CGAATCG3050.016.58670637
TATCAAC87950.016.2352752
TGCGATA2051.05501385E-1016.10022213
ATCAACG89000.016.0194763
TCAACGC89350.015.9815784
CAACGCA90000.015.6957015
GCGATAC1857.565177E-915.46201614
GTATACG1004.9424224E-415.4018821
GTACCGA4150.015.3744896
AACGCAG92800.015.3156466
AACGTCG1057.157757E-414.66824822
TATTACG1057.160097E-414.6676152
GTACATG91250.014.3469581
TACATGG89550.014.1270992
ACTACGC1251.6711192E-414.0813153
ACATGGG93800.013.1355553
Produced by FastQC (version 0.11.5)