##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077415_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3480269
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.50555776004671	31.0	31.0	34.0	27.0	34.0
2	30.4338860013407	31.0	31.0	34.0	26.0	34.0
3	30.612926184728824	31.0	31.0	34.0	27.0	34.0
4	34.085722109411655	37.0	35.0	37.0	30.0	37.0
5	33.71714542755172	37.0	35.0	37.0	28.0	37.0
6	33.6768505537934	37.0	35.0	37.0	28.0	37.0
7	33.49409054300113	37.0	35.0	37.0	28.0	37.0
8	33.45718908509658	37.0	35.0	37.0	27.0	37.0
9	34.90001491264037	39.0	35.0	39.0	27.0	39.0
10	34.755759396759274	39.0	35.0	39.0	27.0	39.0
11	34.71611045008302	39.0	35.0	39.0	27.0	39.0
12	34.633122612073954	39.0	35.0	39.0	27.0	39.0
13	34.60624537930833	39.0	35.0	39.0	27.0	39.0
14	35.76213562802186	40.0	36.0	41.0	27.0	41.0
15	35.77197164931791	40.0	36.0	41.0	27.0	41.0
16	35.71888983294107	40.0	36.0	41.0	27.0	41.0
17	35.689281202113975	40.0	36.0	41.0	27.0	41.0
18	35.68465799626408	40.0	36.0	41.0	27.0	41.0
19	35.72243151319625	40.0	36.0	41.0	27.0	41.0
20	35.71319745686325	40.0	36.0	41.0	27.0	41.0
21	35.65309233280531	40.0	36.0	41.0	26.0	41.0
22	35.659997258832576	40.0	36.0	41.0	27.0	41.0
23	35.595028142939526	40.0	36.0	41.0	26.0	41.0
24	35.582007597688566	40.0	36.0	41.0	26.0	41.0
25	35.56244761539985	40.0	36.0	41.0	26.0	41.0
26	35.47960603045339	40.0	35.0	41.0	26.0	41.0
27	35.42291558497346	40.0	35.0	41.0	25.0	41.0
28	35.35532942999521	40.0	35.0	41.0	25.0	41.0
29	35.27512212418063	40.0	35.0	41.0	25.0	41.0
30	35.14706219547972	39.0	35.0	41.0	24.0	41.0
31	35.06685230365814	39.0	35.0	41.0	24.0	41.0
32	34.98015124692947	39.0	35.0	41.0	24.0	41.0
33	34.88678001614243	39.0	35.0	41.0	24.0	41.0
34	34.85990881739314	39.0	35.0	41.0	24.0	41.0
35	34.79000646214416	39.0	34.0	41.0	23.0	41.0
36	34.68041608277981	39.0	34.0	41.0	23.0	41.0
37	34.59260361770886	39.0	34.0	41.0	22.0	41.0
38	34.505395128939746	39.0	34.0	41.0	21.0	41.0
39	34.413957369387255	39.0	34.0	41.0	20.0	41.0
40	34.31911498795064	39.0	34.0	41.0	19.0	41.0
41	34.25222102084638	39.0	34.0	41.0	18.0	41.0
42	34.13631503771691	39.0	34.0	41.0	18.0	41.0
43	34.2323708311053	39.0	34.0	41.0	18.0	41.0
44	34.16297878123789	39.0	34.0	41.0	15.0	41.0
45	34.09043467617015	39.0	34.0	41.0	15.0	41.0
46	33.937701654670946	39.0	34.0	41.0	9.0	41.0
47	33.78333226540822	39.0	33.0	41.0	2.0	41.0
48	33.62175768597198	39.0	33.0	41.0	2.0	41.0
49	33.46603983772519	39.0	33.0	41.0	2.0	41.0
50	33.316843899135385	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6516540990984474
1101	2	0.8227876697655674
1101	3	0.7011093536617423
1101	4	0.7596932212505934
1101	5	0.8072608886668107
1101	6	0.8209964704189829
1101	7	0.7695859152124811
1101	8	0.789423732574555
1101	9	0.8273982941924487
1101	10	0.9731641319721263
1101	11	0.9981403943695
1101	12	0.9449041348809786
1101	13	1.0034984791577344
1101	14	1.0958857043016295
1101	15	1.1696407311267478
1101	16	1.1899763653902085
1101	17	1.2417089766897575
1101	18	1.1568043809272552
1101	19	1.140273087919475
1101	20	1.1221816238754059
1101	21	1.1371626001049506
1101	22	1.1730201301938052
1101	23	1.263725742836641
1101	24	1.243869790033898
1101	25	1.242002143687536
1101	26	1.2120802937370598
1101	27	1.1645119066817742
1101	28	1.1855751275826236
1101	29	1.281049324855239
1101	30	1.3534364280242386
1101	31	1.3844362526379683
1101	32	1.3264927914436413
1101	33	1.3029078076325362
1101	34	1.274969500460557
1101	35	1.254616419600687
1101	36	1.2506206721307933
1101	37	1.234522051620182
1101	38	1.386270395899949
1101	39	1.2913559701553226
1101	40	1.2341410639983579
1101	41	1.3164701473203948
1101	42	1.270319248000007
1101	43	1.2940273783928475
1101	44	1.3674577196330873
1101	45	1.3107461805106695
1101	46	1.348657321574592
1101	47	1.296886083040988
1101	48	1.3715257548452584
1101	49	1.3937465505389497
1101	50	1.4588618469533614
1102	1	0.4496394415486762
1102	2	0.5394470328421406
1102	3	0.4608622908382749
1102	4	0.4955913739459987
1102	5	0.49409667122130685
1102	6	0.4243201076068459
1102	7	0.5413130883453405
1102	8	0.4865934654898183
1102	9	0.544949576728051
1102	10	0.5535717478536597
1102	11	0.5691811559576507
1102	12	0.5997350819551386
1102	13	0.622207254799001
1102	14	0.7246054353280371
1102	15	0.67556155013623
1102	16	0.7211387695305902
1102	17	0.766480235803499
1102	18	0.8201393512721609
1102	19	0.8061893736661361
1102	20	0.7413467479734734
1102	21	0.7935790558489728
1102	22	0.659112079874582
1102	23	0.6233747927403144
1102	24	0.664164074345031
1102	25	0.6264575854917709
1102	26	0.6521630257834516
1102	27	0.6493432315278298
1102	28	0.69520377780362
1102	29	0.6691064439667187
1102	30	0.846651266904523
1102	31	0.7816433849714599
1102	32	0.716422148098097
1102	33	0.7659632267704666
1102	34	0.82895499401954
1102	35	0.6679442122794654
1102	36	0.6569438182151828
1102	37	0.7509536182402528
1102	38	0.7432912866860875
1102	39	0.6865076455737054
1102	40	0.7655826415738147
1102	41	0.7353122764428264
1102	42	0.6717200262101244
1102	43	0.72911785476321
1102	44	0.6939633295458236
1102	45	0.6060667199689433
1102	46	0.7622552211628175
1102	47	0.7957040347617408
1102	48	0.8775738829854873
1102	49	0.9312370117984443
1102	50	0.8465774602660474
1103	1	0.5872650619750459
1103	2	0.7392437935949516
1103	3	0.6373298219831511
1103	4	0.5945323432040013
1103	5	0.6745539520389343
1103	6	0.6667655085887461
1103	7	0.7015879780390009
1103	8	0.7645428309100879
1103	9	0.8065779469499859
1103	10	0.840069892190968
1103	11	0.904243595487614
1103	12	0.8704578468476143
1103	13	0.8296435347269906
1103	14	0.9741287142345882
1103	15	0.9199502298449573
1103	16	0.9557184971270942
1103	17	0.9536235936094144
1103	18	0.949473349588736
1103	19	0.930972729914437
1103	20	0.9751184610242234
1103	21	0.9222452252759226
1103	22	0.9333823206764009
1103	23	1.037485916831578
1103	24	0.9516761683532948
1103	25	0.9952229931834111
1103	26	1.1052065222773138
1103	27	0.9597867633676458
1103	28	1.057349606831039
1103	29	1.0949787851570392
1103	30	1.1352740626498274
1103	31	1.031017192419874
1103	32	1.0545740638189613
1103	33	1.1157095146962277
1103	34	1.0099900913557178
1103	35	1.1152164330539023
1103	36	1.0668823222425274
1103	37	1.1236852615576254
1103	38	1.0027442331309757
1103	39	1.0824325264308712
1103	40	1.0640477883643698
1103	41	1.0720699739233766
1103	42	1.0647149369915354
1103	43	1.1333885748322245
1103	44	1.074136195234182
1103	45	1.1019011391420293
1103	46	1.132470112593822
1103	47	1.1498014697835899
1103	48	1.0687155274111575
1103	49	1.1472060451464543
1103	50	1.168995033796115
1104	1	0.4930448716703921
1104	2	0.6454597829614173
1104	3	0.5099587040585831
1104	4	0.4763404875612949
1104	5	0.47771173141754275
1104	6	0.5059210915067354
1104	7	0.5435721839425653
1104	8	0.5033723989758627
1104	9	0.5821395809991685
1104	10	0.4790548210852421
1104	11	0.5078252501691551
1104	12	0.5919125853165284
1104	13	0.6250491258260737
1104	14	0.7852381188297031
1104	15	0.7526953242053551
1104	16	0.7667383096323732
1104	17	0.8129659033138523
1104	18	0.7488793874847346
1104	19	0.6615315736645186
1104	20	0.7904841080136435
1104	21	0.8227620087395451
1104	22	0.8173181680859471
1104	23	0.7319055149848737
1104	24	0.7426662220314597
1104	25	0.672033527875115
1104	26	0.7457169892077218
1104	27	0.8345908715722032
1104	28	0.798171944407926
1104	29	0.7846815997594447
1104	30	0.8625471237773752
1104	31	0.7607032768630333
1104	32	0.7658867886445648
1104	33	0.7865466858725298
1104	34	0.8126151177715641
1104	35	0.8878642152788032
1104	36	0.8620199006410587
1104	37	0.8496830171012419
1104	38	0.8633250880285885
1104	39	0.9007951451785701
1104	40	0.9227451004707774
1104	41	0.8400608945795511
1104	42	0.883948569745705
1104	43	0.8601108482256876
1104	44	0.9219796656361439
1104	45	0.9394160669149514
1104	46	0.9110278457510503
1104	47	0.9590404213408874
1104	48	1.0079796534730434
1104	49	1.0277114317981315
1104	50	0.9772119301370523
1105	1	0.41380924009052933
1105	2	0.5380699668742999
1105	3	0.4370188713175054
1105	4	0.4780086061208664
1105	5	0.5700281550882735
1105	6	0.5119264401182946
1105	7	0.5384135400141261
1105	8	0.5070175708211622
1105	9	0.5207148388095675
1105	10	0.634813770609739
1105	11	0.6559610275544614
1105	12	0.7095409868494187
1105	13	0.6755837039829444
1105	14	0.713086922219901
1105	15	0.7674850010569045
1105	16	0.7863421237726058
1105	17	0.8420842192241125
1105	18	0.7959932944837718
1105	19	0.7600606517515729
1105	20	0.8562528507402973
1105	21	0.9399765509390505
1105	22	0.7779582218027556
1105	23	0.8576597882177239
1105	24	0.804719902879107
1105	25	0.8105550373479531
1105	26	0.7313990253053717
1105	27	0.7585943260241379
1105	28	0.8427439462239903
1105	29	0.8795308296543851
1105	30	0.9944032519010335
1105	31	0.9918321975739204
1105	32	0.9885827657083084
1105	33	1.0148721242471126
1105	34	0.8693154584666729
1105	35	1.0230540012892035
1105	36	1.0016991630608345
1105	37	1.0255947990420466
1105	38	1.0685450460429848
1105	39	0.9948472131462154
1105	40	1.0360825906670144
1105	41	0.9828482395184253
1105	42	1.069697329152831
1105	43	0.988277169454328
1105	44	1.0307187068537829
1105	45	0.9994170369929236
1105	46	1.1120483477112373
1105	47	1.1388540445742095
1105	48	1.1183813682040693
1105	49	1.1542903429479452
1105	50	1.2481219284670004
1106	1	0.30954786208577545
1106	2	0.45407876253127455
1106	3	0.32694789103154775
1106	4	0.3544880556411272
1106	5	0.35095246732470997
1106	6	0.4195687341338754
1106	7	0.4048389962024004
1106	8	0.40874676164249024
1106	9	0.42117694371083303
1106	10	0.4364839339000355
1106	11	0.5244194764193537
1106	12	0.506423112540503
1106	13	0.38906362021762675
1106	14	0.5183183705348569
1106	15	0.6039424614347269
1106	16	0.6253425055522612
1106	17	0.5869032516122203
1106	18	0.546486310946392
1106	19	0.46751830867442834
1106	20	0.5568077436766075
1106	21	0.5805224681054568
1106	22	0.6657835054326071
1106	23	0.6847368648141625
1106	24	0.5870589765651388
1106	25	0.6301793012910721
1106	26	0.6314843655427467
1106	27	0.6030417367834104
1106	28	0.593117456477728
1106	29	0.6092042640003399
1106	30	0.7504071051226546
1106	31	0.7654026098852782
1106	32	0.6957316271235712
1106	33	0.7408935768920273
1106	34	0.7099936220574392
1106	35	0.6659580320415657
1106	36	0.7070139242756923
1106	37	0.693517668027603
1106	38	0.706382653750623
1106	39	0.6421079921260997
1106	40	0.6724198619433324
1106	41	0.656395369459446
1106	42	0.7565282144228149
1106	43	0.6967459342658913
1106	44	0.6060953274700083
1106	45	0.6364916188972458
1106	46	0.6108282106589513
1106	47	0.6366479812986583
1106	48	0.6737989581095505
1106	49	0.7543754057602001
1106	50	0.7929015451465631
1107	1	0.3344438936344609
1107	2	0.5502006298016937
1107	3	0.31349499376108625
1107	4	0.4352985497671895
1107	5	0.3927384230989617
1107	6	0.3599027698093664
1107	7	0.36953985515377497
1107	8	0.3268786885465573
1107	9	0.35850418717054566
1107	10	0.4120216618885877
1107	11	0.3897935892216111
1107	12	0.4563089666714504
1107	13	0.46495524140654965
1107	14	0.4817579102171905
1107	15	0.38410720211342664
1107	16	0.4968727302581257
1107	17	0.57383993175862
1107	18	0.5059784608550046
1107	19	0.4859068652958527
1107	20	0.5074008607234504
1107	21	0.524964812794046
1107	22	0.44008357566681866
1107	23	0.5706257202581853
1107	24	0.5106541657394743
1107	25	0.46446319584377704
1107	26	0.5328118358458909
1107	27	0.5046527962730991
1107	28	0.4167279837578377
1107	29	0.3941087062054365
1107	30	0.6104976156512407
1107	31	0.5679702332204926
1107	32	0.5309895175669226
1107	33	0.5979107428323616
1107	34	0.5176816250936511
1107	35	0.6085960989865526
1107	36	0.5926166080079653
1107	37	0.6009758786691748
1107	38	0.5586488108920449
1107	39	0.5521389911532921
1107	40	0.49530972509566595
1107	41	0.49719717373719163
1107	42	0.38706343404651733
1107	43	0.42356903565181625
1107	44	0.45789980588280343
1107	45	0.47989587570145886
1107	46	0.4851973983413558
1107	47	0.5088309915982236
1107	48	0.5227878907675816
1107	49	0.6148122538579841
1107	50	0.7325714879558447
1108	1	0.3896945301048582
1108	2	0.4972137878092191
1108	3	0.3774103710573087
1108	4	0.37155176640713705
1108	5	0.36508037699879026
1108	6	0.3985297424389955
1108	7	0.3895815493222514
1108	8	0.4081692190671333
1108	9	0.43703796510452264
1108	10	0.4255515224222748
1108	11	0.45624459600205824
1108	12	0.434042086276456
1108	13	0.4841284556463421
1108	14	0.4794286600093187
1108	15	0.47882778089432065
1108	16	0.3849223106591211
1108	17	0.4113827874319895
1108	18	0.4028876406011008
1108	19	0.5023910456848526
1108	20	0.44933953669358573
1108	21	0.47511614273858527
1108	22	0.4551896197782881
1108	23	0.5438118439177373
1108	24	0.5419847274621006
1108	25	0.5306886906620036
1108	26	0.5007097262786857
1108	27	0.5143992881854302
1108	28	0.4390659431094477
1108	29	0.5352079784433244
1108	30	0.646206442231275
1108	31	0.6884921646616959
1108	32	0.5862903859096988
1108	33	0.5152015819446447
1108	34	0.5093966920837687
1108	35	0.5260992834815355
1108	36	0.6621159179626162
1108	37	0.6503997208073713
1108	38	0.6660692070895067
1108	39	0.6156469245317666
1108	40	0.5799154906174024
1108	41	0.5777270942748274
1108	42	0.6040973534183607
1108	43	0.5022700360032673
1108	44	0.6425052971007972
1108	45	0.6336446306196422
1108	46	0.6205238112199183
1108	47	0.6058595620471436
1108	48	0.5762047724483779
1108	49	0.6417843691684197
1108	50	0.5150933679651075
1109	1	0.08446470717584376
1109	2	0.1937120191207633
1109	3	0.1493382198166202
1109	4	0.13374689548540175
1109	5	0.14496132493522396
1109	6	0.09925393216626333
1109	7	0.11252588257836749
1109	8	0.08848245042715064
1109	9	0.21572010055012214
1109	10	0.13117055375620623
1109	11	0.14291660080830582
1109	12	0.1894414350151763
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2211	4	-0.16188859776056574
2211	5	-0.20970684127097883
2211	6	-0.176330275802961
2211	7	-0.23002061248391215
2211	8	-0.16416737763207578
2211	9	-0.3711925879448046
2211	10	-0.28652751823261013
2211	11	-0.3395071226567552
2211	12	-0.36624747511336864
2211	13	-0.2842975636631664
2211	14	-0.35983992473124715
2211	15	-0.3922001746946364
2211	16	-0.3787462004955202
2211	17	-0.37846667181673865
2211	18	-0.37590308325708577
2211	19	-0.3185799687364721
2211	20	-0.2731446381377083
2211	21	-0.22956207805407303
2211	22	-0.35261858686332914
2211	23	-0.2612274327662476
2211	24	-0.2507461586820696
2211	25	-0.19259801561015166
2211	26	-0.18523978584160972
2211	27	-0.192132034918437
2211	28	-0.23434978496154457
2211	29	-0.17712955872762137
2211	30	-0.24211327180137943
2211	31	-0.26018878376541466
2211	32	-0.22957816971087652
2211	33	-0.2655465486679631
2211	34	-0.203895836854052
2211	35	-0.2582831842603497
2211	36	-0.27735859811738806
2211	37	-0.21123091065013
2211	38	-0.19232652130385475
2211	39	-0.216026951308109
2211	40	-0.2176863330984986
2211	41	-0.1820090860858059
2211	42	-0.29116972923165463
2211	43	-0.25566865776141157
2211	44	-0.2577991040271854
2211	45	-0.3089839958746765
2211	46	-0.24243393775432764
2211	47	-0.22651327345249683
2211	48	-0.1814838837174193
2211	49	-0.27071266188486476
2211	50	-0.2382391456747328
2212	1	-0.8922056342859221
2212	2	-2.3071511743944733
2212	3	-1.1357984395098235
2212	4	-0.5563426091742087
2212	5	-0.6041442511682646
2212	6	-0.529272329991251
2212	7	-0.6129290758226915
2212	8	-0.6745283033353218
2212	9	-0.7096574141373466
2212	10	-0.646995772403038
2212	11	-0.6656673014799566
2212	12	-0.7072002435304867
2212	13	-2.1967809562657052
2212	14	-1.6152284442382694
2212	15	-1.2386035166672542
2212	16	-1.0360357213293838
2212	17	-0.8465671941999204
2212	18	-1.0127878954169773
2212	19	-0.8221558479833533
2212	20	-0.8307937061440427
2212	21	-0.7988908169121487
2212	22	-0.7993132350385537
2212	23	-0.837098479715003
2212	24	-0.7867600230318175
2212	25	-0.7814109191421039
2212	26	-0.8648163046837567
2212	27	-0.8869943831431044
2212	28	-0.844674388966645
2212	29	-0.8635347450144479
2212	30	-0.8727716751696946
2212	31	-0.8243109644733906
2212	32	-0.8677563206501162
2212	33	-0.8056620629597546
2212	34	-0.8819431678443976
2212	35	-0.8855100810827352
2212	36	-0.789675410970375
2212	37	-0.8573511732444459
2212	38	-0.8287720990127596
2212	39	-0.7738676088787173
2212	40	-0.7809864026837161
2212	41	-0.7578765035532484
2212	42	-0.8071105455651804
2212	43	-0.8327694603539939
2212	44	-0.9055423832863951
2212	45	-0.9789339204587577
2212	46	-0.876500894559932
2212	47	-0.8976426450367114
2212	48	-0.7843201620499727
2212	49	-0.9011227146339991
2212	50	-0.9635637305242426
2213	1	-0.8294199558680013
2213	2	-1.994096438355367
2213	3	-1.1329149819970645
2213	4	-0.7068433963840377
2213	5	-0.8183513406398788
2213	6	-0.6275493040959859
2213	7	-0.6737041263655783
2213	8	-0.597829724624134
2213	9	-0.629565446790437
2213	10	-0.5547211032324313
2213	11	-0.6446022117282553
2213	12	-0.6309817762142345
2213	13	-0.6765041093596764
2213	14	-0.7145840936322259
2213	15	-0.8719753665754055
2213	16	-0.9235741637279133
2213	17	-0.9727030254026303
2213	18	-0.8749300875188979
2213	19	-0.8745618851078163
2213	20	-1.0838115848615573
2213	21	-0.909183006515903
2213	22	-0.9896658161340071
2213	23	-0.8552823157213751
2213	24	-0.9256630365012839
2213	25	-1.0196779838688528
2213	26	-0.8109401825141873
2213	27	-0.8900018025737992
2213	28	-0.8838251093386233
2213	29	-0.8866790893363046
2213	30	-0.9512514489061417
2213	31	-0.9365378208435757
2213	32	-0.86425818419373
2213	33	-0.8531432652539976
2213	34	-1.033729715614136
2213	35	-0.9563948458427873
2213	36	-0.9890524196914754
2213	37	-1.0547395917648075
2213	38	-0.9964537670112747
2213	39	-1.0101989738230515
2213	40	-1.0674334017154692
2213	41	-0.9682546159830636
2213	42	-1.195042722005546
2213	43	-1.157863515598379
2213	44	-1.1334670641627937
2213	45	-1.072902047163815
2213	46	-1.0697132426682785
2213	47	-1.1056688555446357
2213	48	-1.109532049984125
2213	49	-1.025878402049834
2213	50	-1.1558839660298474
2214	1	-0.8786744934184441
2214	2	-1.837420273231082
2214	3	-1.0333184837310938
2214	4	-0.6440405975196342
2214	5	-0.7506368998984527
2214	6	-0.6559202956061156
2214	7	-0.6498623616284931
2214	8	-0.7160529040007049
2214	9	-0.8122723326172974
2214	10	-0.7473957527720998
2214	11	-0.7208315297774348
2214	12	-0.7200740062647668
2214	13	-0.758462785249236
2214	14	-0.8274595520431163
2214	15	-0.8825783684020081
2214	16	-0.843361935864543
2214	17	-0.7766100206417619
2214	18	-0.9164858734303252
2214	19	-0.9168673765102113
2214	20	-0.9662652619475693
2214	21	-1.0006251844859761
2214	22	-0.9220285554361283
2214	23	-0.950418071391482
2214	24	-0.8328236324381422
2214	25	-0.8943010840419845
2214	26	-0.9811238026690035
2214	27	-0.8644104092212572
2214	28	-0.9423157058926463
2214	29	-0.9142645768083
2214	30	-0.8713445576113727
2214	31	-0.8567110533292208
2214	32	-0.8891921429797947
2214	33	-0.8620732432957539
2214	34	-0.928764247339366
2214	35	-0.9833690172925174
2214	36	-1.0211138421484378
2214	37	-0.8591102086421287
2214	38	-1.0568241933239193
2214	39	-1.07486542881135
2214	40	-0.952560803940095
2214	41	-0.9663008882783117
2214	42	-0.8819541438128624
2214	43	-0.9034789379643939
2214	44	-0.9082532608926641
2214	45	-1.0671982355456535
2214	46	-0.9832434761316478
2214	47	-0.9477706335887746
2214	48	-0.8541346238522962
2214	49	-0.8093183730969997
2214	50	-0.9028040390399354
2215	1	-0.5262852361545463
2215	2	-0.2574477862393252
2215	3	-0.5254611599755101
2215	4	-0.4552118573621513
2215	5	-0.6057714143341499
2215	6	-0.6663556688160384
2215	7	-0.7567987478696807
2215	8	-0.6878399832473789
2215	9	-0.9349058109982877
2215	10	-0.941652268405015
2215	11	-0.8349212981616532
2215	12	-0.7361904127665824
2215	13	-0.7177990770943339
2215	14	-0.8561697790542127
2215	15	-0.9052437918030307
2215	16	-0.9733436322314475
2215	17	-1.0013043845041736
2215	18	-0.9766354682178147
2215	19	-0.8911789757182049
2215	20	-0.9815355571080389
2215	21	-0.9149638937920486
2215	22	-0.9961772638212665
2215	23	-0.9628150415070635
2215	24	-1.0017405523626195
2215	25	-0.9904921440410632
2215	26	-1.0487991837068975
2215	27	-0.9834959758280633
2215	28	-1.1757995549509914
2215	29	-1.0195924881220222
2215	30	-1.0272322262580573
2215	31	-1.0635818227725125
2215	32	-0.9950967914118962
2215	33	-0.9612959144750164
2215	34	-1.0018331564888783
2215	35	-1.0389136367150869
2215	36	-0.9332471939005202
2215	37	-0.9225892803371138
2215	38	-0.9415400841007298
2215	39	-0.9235168309387163
2215	40	-0.853688952395224
2215	41	-0.9023299783920464
2215	42	-0.8707656163919424
2215	43	-0.8673243701143178
2215	44	-0.8524887676385262
2215	45	-0.9272306985930356
2215	46	-0.9039176599507783
2215	47	-0.9150511626673747
2215	48	-0.9171771136068685
2215	49	-0.7734633977320797
2215	50	-0.8767446731054136
2216	1	-1.0877029403713365
2216	2	-0.6966201564759587
2216	3	-0.8525280795748174
2216	4	-2.293352207516726
2216	5	-1.3706207950839584
2216	6	-1.255618083376909
2216	7	-1.1982682590369365
2216	8	-1.0329767888363008
2216	9	-1.1729495951939626
2216	10	-1.2034297685365871
2216	11	-1.3504640697877335
2216	12	-1.323817308140491
2216	13	-1.39711908798661
2216	14	-1.4659688593130298
2216	15	-1.4849069703486037
2216	16	-1.4842561195218877
2216	17	-1.4896720002784463
2216	18	-1.5522962699898741
2216	19	-1.4352051965033752
2216	20	-1.5801306978418665
2216	21	-1.5311659225924998
2216	22	-1.5434556064260008
2216	23	-1.4759207812320554
2216	24	-1.6109320145893875
2216	25	-1.6889319096893658
2216	26	-1.8803306389620005
2216	27	-1.8019974338519944
2216	28	-1.7670384443356753
2216	29	-1.7221581983585423
2216	30	-1.6794789866074282
2216	31	-1.6155136377472559
2216	32	-1.5722619169133196
2216	33	-1.6667755368456696
2216	34	-1.6369569264361203
2216	35	-1.7212395491146637
2216	36	-1.7184825181249224
2216	37	-1.735278779589983
2216	38	-1.8352835100260236
2216	39	-1.869787409365756
2216	40	-1.7364200451847367
2216	41	-1.9372595485514879
2216	42	-1.9385212053565795
2216	43	-1.739774773273382
2216	44	-1.7034179289563127
2216	45	-1.6235054340854447
2216	46	-1.5598435375682982
2216	47	-1.5427076227851018
2216	48	-1.5378223969394753
2216	49	-1.5677193249826722
2216	50	-1.5158038491216672
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	87581.0
3	13542.0
4	23462.0
5	14006.0
6	10473.0
7	8241.0
8	6872.0
9	6188.0
10	5868.0
11	5810.0
12	6070.0
13	6422.0
14	6853.0
15	7509.0
16	8319.0
17	8904.0
18	9623.0
19	10601.0
20	12551.0
21	15769.0
22	19676.0
23	23940.0
24	28098.0
25	31424.0
26	34576.0
27	39185.0
28	45864.0
29	52765.0
30	61020.0
31	72547.0
32	88715.0
33	109573.0
34	138062.0
35	176023.0
36	229609.0
37	310129.0
38	492752.0
39	1251647.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.47984575743869	18.215088941217584	13.973562195053962	26.331503106289773
2	14.735615637359906	20.0245096982836	38.12775213310519	27.112122531251302
3	17.778229213927386	24.575497571870535	29.3621638209428	28.28410939325928
4	12.30132173932033	16.471471216920243	36.67409251284486	34.55311453091458
5	13.76385237129831	37.66338777268903	34.293948375313356	14.27881148069931
6	32.60704240653782	36.891382861373565	17.05041959324259	13.451155138846016
7	28.285733830223748	31.779343637888523	21.62355665234744	18.31136587954029
8	26.773691152028782	34.53514101210736	19.534133669861326	19.15703416600253
9	26.590080337179895	15.092013677813734	18.782160668036518	39.53574531696986
10	14.358207007596931	28.724145750980046	33.73597760613388	23.181669635289136
11	35.281380366167156	22.66375348780923	23.286507269676676	18.76835887634694
12	23.588066224400308	24.511979221012165	30.220336951432046	21.67961760315548
13	28.325313246179263	21.477229055816185	25.873181539494556	24.324276158509996
14	22.40315672887506	20.387628920507154	27.126924267655056	30.082290082962732
15	23.71453110753661	28.58931566983734	23.758136042798945	23.9380171798271
16	23.728516355439062	27.55658568262	25.162306816440726	23.55259114550021
17	22.53601859781084	27.365194186660553	26.61838985893824	23.48039735659037
18	22.648736397853497	26.83282033768309	27.548225759482225	22.97021750498119
19	23.476030281896236	26.528454391731643	27.636082534093582	22.35943279227854
20	23.725571025131543	25.945307613055157	27.561247792250796	22.767873569562497
21	23.856312770597928	25.793420932726853	27.56724684567729	22.783019450997934
22	23.55081941765435	25.544213482661576	27.499978440159943	23.404988659524133
23	23.342776448267685	25.674952748492625	27.411451034502583	23.57081976873711
24	23.10248668866849	25.788123098663533	27.46378567570512	23.645604536962857
25	23.37619966908852	25.69554074361949	27.521590306754252	23.406669280537738
26	23.178977566719176	26.036202554933368	27.542342673826305	23.24247720452115
27	23.260771579954376	26.09627387040989	27.288874503274705	23.354080046361027
28	23.285295585364114	25.94736908674046	27.408882163693605	23.358453164201826
29	23.344944430008024	26.02910267053307	27.36517406871684	23.26077883074206
30	23.344129675684314	26.02783708675821	27.348290694104783	23.27974254345269
31	23.408996185718433	25.6751450835434	27.346587985731656	23.56927074500651
32	23.192335616836026	26.01388036990559	27.33063574577527	23.46314826748311
33	23.36916254487034	25.86319180794776	27.211902962232752	23.555742684949145
34	23.49176506022019	25.904308700745265	27.307789136799897	23.29613710223465
35	23.49315267307423	25.91988637555672	27.18361918107976	23.403341770289288
36	23.501241702464466	25.998614167507544	27.060261871184256	23.439882258843735
37	23.594594377113008	25.679898702048142	27.253252926455524	23.472253994383323
38	23.53799623405855	25.83647353380036	27.2231527229504	23.402377509190693
39	23.61228056764606	26.111638284555262	27.00721368686587	23.268867460932807
40	23.653942031525908	25.78489336909967	27.25851617602899	23.302648423345435
41	23.35628345895558	25.873402662636025	27.15543920850802	23.614874669900374
42	23.61023907476952	25.912309653823044	27.015400105918292	23.462051165489143
43	23.44426418587972	25.77074903467803	27.072690942380667	23.712295837061582
44	23.48690496202015	26.03594400791923	26.862090120067183	23.615060909993435
45	23.593803202990646	26.064964937208796	26.694098158574903	23.647133701225652
46	23.6520976564554	25.95007069912687	26.62295513442891	23.77487650998882
47	23.620375271028756	26.0535282154369	26.579581168982298	23.74651534455204
48	23.516919445006344	26.34610787252432	26.487275648687913	23.649697033781422
49	23.66474286735554	26.061858490126905	26.565842955682122	23.707555686835434
50	23.75579086982794	26.123600429048032	26.442110840473838	23.678497860650186
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	575.0
1	449.0
2	323.0
3	502.0
4	681.0
5	772.0
6	863.0
7	856.5
8	850.0
9	1107.5
10	1365.0
11	2120.0
12	2875.0
13	5432.5
14	7990.0
15	10941.0
16	13892.0
17	16039.0
18	18186.0
19	19412.5
20	20639.0
21	22574.5
22	24510.0
23	28410.0
24	32310.0
25	38375.5
26	44441.0
27	51664.5
28	58888.0
29	66282.5
30	73677.0
31	82261.5
32	90846.0
33	100928.0
34	111010.0
35	122501.5
36	133993.0
37	143525.5
38	153058.0
39	163149.0
40	173240.0
41	184297.0
42	195354.0
43	202520.5
44	209687.0
45	219841.5
46	229996.0
47	236727.5
48	243459.0
49	243385.0
50	243311.0
51	244554.0
52	245797.0
53	240319.0
54	234841.0
55	225126.5
56	215412.0
57	202229.5
58	189047.0
59	172430.0
60	155813.0
61	137010.5
62	118208.0
63	101515.5
64	84823.0
65	71375.0
66	57927.0
67	47472.0
68	37017.0
69	30025.0
70	23033.0
71	18244.5
72	13456.0
73	11098.0
74	8740.0
75	6638.5
76	4537.0
77	3511.0
78	2485.0
79	1977.5
80	1470.0
81	1128.0
82	786.0
83	611.0
84	436.0
85	333.0
86	230.0
87	164.0
88	98.0
89	76.0
90	54.0
91	34.5
92	15.0
93	12.0
94	9.0
95	9.0
96	9.0
97	6.0
98	3.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015947043173961555
2	4.022677557395707E-4
3	5.17201400236591E-4
4	0.0023561397121889143
5	0.03884757183999283
6	0.02721054033466953
7	0.029624146869106957
8	0.02344646347739212
9	0.03039994896946184
10	0.03166421905892906
11	0.039249839595732396
12	0.03936477324022942
13	0.037238500817034545
14	0.042467981641648965
15	0.03749710151715284
16	0.043617318086619165
17	0.04539878957632298
18	0.048329597510997
19	0.050427136523067614
20	0.046346992143423395
21	0.046835460132535735
22	0.04537005616519873
23	0.0443643867758498
24	0.042123180708157903
25	0.040025641696087286
26	0.04335871738650087
27	0.042669115519518755
28	0.04243924823052471
29	0.040600309918572386
30	0.03772696880614688
31	0.035600696382952005
32	0.031922819759047365
33	0.022957995488279787
34	0.020257054842599813
35	0.02114779058745172
36	0.0221821933879249
37	0.018188249241653447
38	0.01899278475313259
39	0.014223038506506249
40	0.007872954648045884
41	0.007441953481182058
42	0.006551217736330151
43	0.002384873123313169
44	0.0038502770906501766
45	0.0024136065344374244
46	8.045355114791414E-4
47	3.160675223668055E-4
48	3.7353434461531563E-4
49	8.620023337276516E-5
50	1.7240046674553032E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3480269.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.48992621461867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.62283501686572	48.3451232397121
2	12.227660263497208	15.03755854759736
3	3.924738999382688	7.239957344510334
4	1.7428316757124778	4.2866636457624185
5	0.9466937234972556	2.9106063602844423
6	0.5986457824002975	2.208641099309218
7	0.37325701476297835	1.6066082407805056
8	0.25571851141697555	1.2579289918993557
9	0.19737918449577616	1.0923148341852194
>10	1.0314101186701385	11.148007398683562
>50	0.05840064860520633	2.458112142293884
>100	0.019725366938716384	1.923593674290262
>500	5.629550052501398E-4	0.2139272611657824
>1k	1.4073874938812863E-4	0.27095721952560414
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4282	0.12303646643406013	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	2.873341112425505E-5	0.0	0.0	0.0	0.0
7	2.873341112425505E-5	0.0	0.0	0.0	0.0
8	8.620023337276514E-5	0.0	0.0	0.0	0.0
9	8.620023337276514E-5	0.0	0.0	0.0	0.0
10	1.149336444970202E-4	0.0	0.0	0.0	0.0
11	1.149336444970202E-4	0.0	0.0	0.0	0.0
12	1.149336444970202E-4	0.0	0.0	0.0	2.873341112425505E-5
13	1.149336444970202E-4	0.0	0.0	0.0	2.873341112425505E-5
14	1.149336444970202E-4	0.0	0.0	2.873341112425505E-5	5.74668222485101E-5
15	1.149336444970202E-4	0.0	0.0	2.873341112425505E-5	8.620023337276514E-5
16	1.149336444970202E-4	0.0	0.0	2.873341112425505E-5	8.620023337276514E-5
17	1.149336444970202E-4	0.0	0.0	2.873341112425505E-5	8.620023337276514E-5
18	1.149336444970202E-4	0.0	0.0	2.873341112425505E-5	8.620023337276514E-5
19	1.149336444970202E-4	0.0	0.0	2.873341112425505E-5	8.620023337276514E-5
20	1.149336444970202E-4	0.0	0.0	2.873341112425505E-5	8.620023337276514E-5
21	1.149336444970202E-4	0.0	0.0	2.873341112425505E-5	8.620023337276514E-5
22	1.149336444970202E-4	0.0	0.0	5.74668222485101E-5	8.620023337276514E-5
23	1.149336444970202E-4	0.0	0.0	5.74668222485101E-5	8.620023337276514E-5
24	1.724004667455303E-4	0.0	0.0	5.74668222485101E-5	8.620023337276514E-5
25	1.724004667455303E-4	0.0	0.0	5.74668222485101E-5	8.620023337276514E-5
26	1.724004667455303E-4	0.0	0.0	1.4366705562127526E-4	8.620023337276514E-5
27	1.724004667455303E-4	0.0	0.0	1.4366705562127526E-4	8.620023337276514E-5
28	2.0113387786978534E-4	0.0	2.873341112425505E-5	2.586007001182955E-4	1.149336444970202E-4
29	2.298672889940404E-4	0.0	2.873341112425505E-5	5.746682224851011E-4	1.149336444970202E-4
30	2.298672889940404E-4	0.0	2.873341112425505E-5	0.0011780698560944572	1.149336444970202E-4
31	2.298672889940404E-4	0.0	2.873341112425505E-5	0.002384873123313169	1.149336444970202E-4
32	2.298672889940404E-4	0.0	2.873341112425505E-5	0.004051410968519962	1.4366705562127526E-4
33	2.298672889940404E-4	0.0	2.873341112425505E-5	0.00597654951384505	1.4366705562127526E-4
34	2.298672889940404E-4	0.0	2.873341112425505E-5	0.008390156048282475	1.4366705562127526E-4
35	2.586007001182955E-4	0.0	2.873341112425505E-5	0.011378430805205	1.4366705562127526E-4
36	3.160675223668056E-4	0.0	2.873341112425505E-5	0.01422303850650625	1.4366705562127526E-4
37	3.160675223668056E-4	0.0	2.873341112425505E-5	0.01853305017514451	1.4366705562127526E-4
38	3.448009334910606E-4	0.0	2.873341112425505E-5	0.024250998988871262	1.724004667455303E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2685	0.0	25.239582	1
GTATCAA	6030	0.0	23.9001	1
TAACGCG	60	0.007407765	18.336365	12
TACCGAT	355	0.0	17.352736	7
CGAATCG	305	0.0	16.586706	37
TATCAAC	8795	0.0	16.235275	2
TGCGATA	205	1.05501385E-10	16.100222	13
ATCAACG	8900	0.0	16.019476	3
TCAACGC	8935	0.0	15.981578	4
CAACGCA	9000	0.0	15.695701	5
GCGATAC	185	7.565177E-9	15.462016	14
GTATACG	100	4.9424224E-4	15.401882	1
GTACCGA	415	0.0	15.374489	6
AACGCAG	9280	0.0	15.315646	6
AACGTCG	105	7.157757E-4	14.668248	22
TATTACG	105	7.160097E-4	14.667615	2
GTACATG	9125	0.0	14.346958	1
TACATGG	8955	0.0	14.127099	2
ACTACGC	125	1.6711192E-4	14.081315	3
ACATGGG	9380	0.0	13.135555	3
>>END_MODULE