FastQCFastQC Report
Mon 6 Feb 2017
SRR2077414_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077414_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3203150
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42000.024.2006111
GGTATCA18700.023.1770551
TATCAAC55350.018.045062
ATCAACG54500.018.0439283
TCAACGC54700.017.8584124
CAACGCA55900.017.4742285
AACGCAG57850.016.997446
GTACATG76200.014.6092921
ACGCAGA67600.014.3192167
TACATGG76400.014.2826352
TATACCG1100.001018569114.0001345
CGCAGAG69200.013.829188
GTATACG1453.8387636E-513.6555171
GTATTAT7900.013.6459141
TTAGACA7750.013.6265754
GTATAGG6050.013.4548851
GTATTAG6450.013.3026611
TAATGCG1000.00876407113.2001275
ACATGGG77800.013.1490213
AGAGTAC68950.012.98715911
Produced by FastQC (version 0.11.5)