##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077414_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3203150
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.182367669325508	31.0	30.0	34.0	23.0	34.0
2	29.156932706866677	31.0	30.0	34.0	22.0	34.0
3	29.269378268267175	31.0	30.0	34.0	22.0	34.0
4	32.642214070524325	37.0	33.0	37.0	24.0	37.0
5	32.71038665064078	37.0	35.0	37.0	25.0	37.0
6	32.73945834569096	37.0	35.0	37.0	25.0	37.0
7	32.586449276493454	37.0	33.0	37.0	23.0	37.0
8	32.49188205360348	37.0	33.0	37.0	22.0	37.0
9	33.72787287513854	39.0	34.0	39.0	19.0	39.0
10	33.63558122473191	38.0	34.0	39.0	19.0	39.0
11	33.69131667265036	38.0	34.0	39.0	19.0	39.0
12	33.61171471832415	38.0	34.0	39.0	18.0	39.0
13	33.62201738913257	38.0	34.0	39.0	18.0	39.0
14	34.498108112326925	39.0	34.0	41.0	17.0	41.0
15	34.593128951188675	39.0	34.0	41.0	17.0	41.0
16	34.49005884832118	39.0	34.0	41.0	17.0	41.0
17	34.31217551472769	39.0	33.0	41.0	17.0	41.0
18	34.13142281816337	39.0	33.0	41.0	17.0	41.0
19	34.4789872469288	39.0	34.0	41.0	18.0	41.0
20	34.513531679752745	39.0	34.0	41.0	18.0	41.0
21	34.51219892917909	39.0	34.0	41.0	18.0	41.0
22	34.418617922982065	39.0	34.0	41.0	18.0	41.0
23	34.163881179463964	39.0	34.0	41.0	17.0	41.0
24	34.31442548741083	39.0	34.0	41.0	18.0	41.0
25	34.220440503879	39.0	34.0	41.0	17.0	41.0
26	34.25784056319561	39.0	34.0	41.0	17.0	41.0
27	34.23631706289122	39.0	34.0	41.0	17.0	41.0
28	34.12836832493015	39.0	34.0	41.0	16.0	41.0
29	34.09242651764669	39.0	34.0	41.0	16.0	41.0
30	33.87781746093689	39.0	33.0	41.0	14.0	41.0
31	33.7959876996082	39.0	33.0	41.0	12.0	41.0
32	33.81433932222968	39.0	33.0	41.0	15.0	41.0
33	33.797482478185536	39.0	33.0	41.0	12.0	41.0
34	33.788340851973835	39.0	33.0	41.0	10.0	41.0
35	33.7211700981846	39.0	33.0	41.0	9.0	41.0
36	33.60596881195074	39.0	33.0	41.0	9.0	41.0
37	33.51269125704385	38.0	33.0	41.0	9.0	41.0
38	33.40795092330987	38.0	33.0	41.0	8.0	41.0
39	33.34356586485178	38.0	33.0	41.0	8.0	41.0
40	33.309132884816506	38.0	33.0	41.0	2.0	41.0
41	33.190715077345736	38.0	33.0	41.0	2.0	41.0
42	33.00877230226496	38.0	32.0	40.0	2.0	41.0
43	32.91544448433573	38.0	32.0	40.0	2.0	41.0
44	32.8494544432824	38.0	32.0	40.0	2.0	41.0
45	32.764011988199115	38.0	32.0	40.0	2.0	41.0
46	32.61653622215631	38.0	32.0	40.0	2.0	41.0
47	32.43724052885441	38.0	32.0	40.0	2.0	41.0
48	32.257257387259415	38.0	32.0	40.0	2.0	41.0
49	32.129107597209	38.0	32.0	40.0	2.0	41.0
50	31.924824001373647	38.0	31.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.0017796755329904
1101	2	1.004896626862191
1101	3	1.0581997482540935
1101	4	1.0501485040025145
1101	5	0.94141741656356
1101	6	1.0500808670851285
1101	7	1.1014151505052183
1101	8	1.1935180394107618
1101	9	1.326347603122251
1101	10	1.370653832629941
1101	11	1.2847060724330177
1101	12	1.2729988827192358
1101	13	1.282473701717926
1101	14	1.5619607900688308
1101	15	1.5559050794294649
1101	16	1.4123718102671745
1101	17	1.6173655015680808
1101	18	1.7847074889935612
1101	19	1.3411988974709885
1101	20	1.447427279671679
1101	21	1.4804477094543884
1101	22	1.2795507095436633
1101	23	1.4477175017805948
1101	24	1.3869573835985207
1101	25	1.487458192929438
1101	26	1.4006060298838676
1101	27	1.4116542993474468
1101	28	1.5218570885633795
1101	29	1.4421713106497478
1101	30	1.6040275838043243
1101	31	1.5825357678523133
1101	32	1.5660465139189341
1101	33	1.5703023736204003
1101	34	1.488793334864745
1101	35	1.4864836409010564
1101	36	1.5924179987277967
1101	37	1.599281239366519
1101	38	1.6153429211032702
1101	39	1.5443134606722353
1101	40	1.4962042289800195
1101	41	1.5695350593231083
1101	42	1.6912930941365616
1101	43	1.6622553173528942
1101	44	1.5695237085234908
1101	45	1.6752565448490557
1101	46	1.5983760804588627
1101	47	1.5679374797178411
1101	48	1.5186475864099762
1101	49	1.7081569629194675
1101	50	1.6845227939918175
1102	1	0.597043170305767
1102	2	0.6022936825481295
1102	3	0.48000474078662947
1102	4	0.5389473092084032
1102	5	0.5509757694545385
1102	6	0.6139810164343942
1102	7	0.6416327737183565
1102	8	0.6424192555404815
1102	9	0.8537638608564109
1102	10	0.9930135509999047
1102	11	0.7412881078501314
1102	12	0.6975775044388826
1102	13	0.7087591721681079
1102	14	1.0586537706534287
1102	15	0.9614950090219594
1102	16	1.1567712128701189
1102	17	1.1878796897481507
1102	18	1.2372357586756593
1102	19	0.9144867148381763
1102	20	0.846403170433355
1102	21	0.8971321557953331
1102	22	1.0600072915790832
1102	23	1.2354281909207643
1102	24	1.0287325062782742
1102	25	0.9792439834137596
1102	26	0.9523448820281004
1102	27	0.9038668020143987
1102	28	0.8517056057385446
1102	29	0.875070819930734
1102	30	1.0384205857245306
1102	31	1.063867109862123
1102	32	1.0480179242027035
1102	33	0.8508656337014742
1102	34	0.8015733647346011
1102	35	0.9018879506941033
1102	36	0.8864226925618084
1102	37	0.9634800872436244
1102	38	1.1423324666547927
1102	39	1.054661231101079
1102	40	1.0592509539640176
1102	41	1.0067016904304253
1102	42	1.026262157503318
1102	43	1.0829935294288546
1102	44	1.1056875660016203
1102	45	1.1467735066583131
1102	46	1.1533152739728365
1102	47	1.0853260011601265
1102	48	1.1026032083429769
1102	49	1.035658563090152
1102	50	1.0911795040408947
1103	1	0.5065022879089547
1103	2	0.5025046393293451
1103	3	0.623306808453318
1103	4	0.5647239567632383
1103	5	0.49893508944109044
1103	6	0.5384641776621137
1103	7	0.6514107858975038
1103	8	0.6231712242545484
1103	9	0.8000878662744881
1103	10	0.8316856757483606
1103	11	0.772445174924556
1103	12	0.7998430897274318
1103	13	0.7094957915294913
1103	14	1.041559109056088
1103	15	0.8550326787024787
1103	16	0.9232850878489742
1103	17	1.0339163165745475
1103	18	0.8499766027814459
1103	19	0.8326440660461145
1103	20	0.8535209753575188
1103	21	0.8929273091978231
1103	22	0.9624175120881446
1103	23	1.161339186487119
1103	24	0.8838043829763436
1103	25	0.7987603100958083
1103	26	0.8987856924500619
1103	27	0.8404368328684626
1103	28	0.9214324062238077
1103	29	0.8619711654536459
1103	30	1.0014734803630745
1103	31	1.1406590996028712
1103	32	0.9656393256214812
1103	33	1.0761897865057435
1103	34	0.9827451370426559
1103	35	0.9326350874023817
1103	36	1.0438655995534631
1103	37	0.9816076540658614
1103	38	0.8945648377460671
1103	39	0.9346294889851592
1103	40	0.9544702773932912
1103	41	0.9371565965099009
1103	42	0.9949427029844671
1103	43	1.070097170856009
1103	44	1.0677222895830667
1103	45	0.9887033514541059
1103	46	0.8985115680313953
1103	47	0.9217987286384286
1103	48	0.9558806325576086
1103	49	0.974930822511574
1103	50	1.0663881316217143
1104	1	0.6083402715986139
1104	2	0.6088949632762315
1104	3	0.6275509625319025
1104	4	0.7232422525277684
1104	5	0.6799496811651977
1104	6	0.7292751959892811
1104	7	0.7153446324542898
1104	8	0.7326804924822454
1104	9	0.9135820133056711
1104	10	0.9138180478205484
1104	11	0.9098365505206232
1104	12	0.8192941747012128
1104	13	0.7706283772469291
1104	14	1.0153967833076578
1104	15	1.000491849559495
1104	16	1.0547008288454904
1104	17	1.2420001794774151
1104	18	1.2750937288544577
1104	19	1.115038571206128
1104	20	1.09269123576685
1104	21	0.9863215355268409
1104	22	0.9603208186422165
1104	23	1.1930351787587625
1104	24	1.0790166919124644
1104	25	1.18066873239367
1104	26	0.9867359150507937
1104	27	1.0902526615160824
1104	28	1.183542312089095
1104	29	1.0930594710465584
1104	30	1.3209343345667506
1104	31	1.3951027285777968
1104	32	1.2116048775814576
1104	33	1.1947609543958109
1104	34	1.1102492144981824
1104	35	1.0644616281854198
1104	36	1.1911966377437224
1104	37	1.129647576587189
1104	38	1.1989160648291204
1104	39	1.099733488977563
1104	40	1.077893985384577
1104	41	1.2347405921370154
1104	42	1.3449972194315478
1104	43	1.2967312601844156
1104	44	1.251205867455809
1104	45	1.1471131194061925
1104	46	1.2788671446881423
1104	47	1.2096583473518194
1104	48	1.182975900334874
1104	49	1.2086829808185087
1104	50	1.294204975526732
1105	1	0.7465922154936102
1105	2	0.7825656120449409
1105	3	0.7785780834270852
1105	4	0.7405693829388227
1105	5	0.7354077498914222
1105	6	0.6719697878280542
1105	7	0.7023225424382602
1105	8	0.6801195562293998
1105	9	0.9454649898278973
1105	10	0.9483737212238452
1105	11	0.9448121411392663
1105	12	1.003457420227825
1105	13	0.9327880876801444
1105	14	1.0483766242335335
1105	15	1.0077556554385367
1105	16	1.0621075420349229
1105	17	1.038537758851085
1105	18	1.3430266829754558
1105	19	1.1328292191079115
1105	20	1.0838372474168452
1105	21	1.1069491898523438
1105	22	1.0467686508703906
1105	23	1.4104837211211319
1105	24	1.2795378294952897
1105	25	1.2313888057572768
1105	26	1.1081045624060124
1105	27	1.0319220542582173
1105	28	1.0740450884530404
1105	29	1.0382484219409633
1105	30	1.3856830716826565
1105	31	1.4697762006124933
1105	32	1.2064402344755365
1105	33	1.2843023743187842
1105	34	1.1740635934795662
1105	35	1.1384001181021617
1105	36	1.1237798600868416
1105	37	1.1235640259297668
1105	38	1.10990495971199
1105	39	1.2449814991964487
1105	40	1.322377111316058
1105	41	1.3831274835406617
1105	42	1.3265316985293225
1105	43	1.2124899828639712
1105	44	1.1789831965317248
1105	45	1.2223442516621645
1105	46	1.2129528409870787
1105	47	1.3850067177851741
1105	48	1.4440526137918326
1105	49	1.3618160373451218
1105	50	1.419567789080439
1106	1	0.4177834904556761
1106	2	0.409059243514168
1106	3	0.4585302925220702
1106	4	0.4371544225497317
1106	5	0.5017552665173355
1106	6	0.40144093981022877
1106	7	0.35961524499703756
1106	8	0.4257715017818384
1106	9	-0.7241199033152554
1106	10	-0.45140766239277497
1106	11	0.18812561461551525
1106	12	0.4972162203056314
1106	13	0.6021676525781174
1106	14	0.7083812999350272
1106	15	0.7256573457493616
1106	16	0.8467074017307397
1106	17	0.9148725788432017
1106	18	-0.7951929848608543
1106	19	0.2698208525514758
1106	20	0.42717374317243184
1106	21	0.6049471876844876
1106	22	0.7604461381464134
1106	23	-0.7291383257889805
1106	24	0.35721660714563797
1106	25	0.6096200849741962
1106	26	0.7056355617104515
1106	27	0.7282812617947485
1106	28	0.7711158824176394
1106	29	0.7521213376335254
1106	30	-0.08401280723821003
1106	31	0.5637508418079094
1106	32	0.7228053553992524
1106	33	0.7125835420445767
1106	34	0.7704307623008901
1106	35	0.7607844094020066
1106	36	0.8879607225831094
1106	37	0.9582103775405315
1106	38	0.9224333544087955
1106	39	0.9019019847668943
1106	40	0.9828970337477898
1106	41	1.0469491852242783
1106	42	1.1380929752377327
1106	43	1.040309222099836
1106	44	1.0711304453403443
1106	45	1.0307603652363113
1106	46	1.026876324507299
1106	47	1.0450571232390757
1106	48	1.0939374692576536
1106	49	1.1221454937012112
1106	50	1.0224200498367892
1107	1	0.4540209883740971
1107	2	0.5430445195199312
1107	3	0.47448679358815227
1107	4	0.48554049621512263
1107	5	0.4364695738854678
1107	6	0.48671464598133696
1107	7	0.4946361943454818
1107	8	0.29826886450314305
1107	9	0.643101901309187
1107	10	0.7083807720409041
1107	11	0.5973496181056603
1107	12	0.6066272503247632
1107	13	0.6082975921683342
1107	14	0.8843845410910092
1107	15	0.7753508365162602
1107	16	0.67873038390875
1107	17	0.9859324893764807
1107	18	0.9121761969461772
1107	19	0.7670462928157775
1107	20	0.7276445301465202
1107	21	0.7109088732440725
1107	22	0.8328539434702193
1107	23	0.9826556270076594
1107	24	0.9376455097186991
1107	25	0.7612033119838202
1107	26	0.8109220745158794
1107	27	0.8043213576619266
1107	28	0.6980485889336023
1107	29	0.6359038065299316
1107	30	0.8923576167968292
1107	31	0.9562687105019592
1107	32	0.8469548797373889
1107	33	0.7623958109342297
1107	34	0.6903569074692371
1107	35	0.6921030021851422
1107	36	0.7968227754767057
1107	37	0.7356434573212383
1107	38	0.6989446783792701
1107	39	0.6686390292412554
1107	40	0.7510766524103829
1107	41	0.683619406384814
1107	42	0.8238786036812229
1107	43	0.7824478810172195
1107	44	0.7318703913828912
1107	45	0.8261326584746627
1107	46	0.8579144040464115
1107	47	0.8798831955925195
1107	48	0.8005771949112273
1107	49	0.8525320213212311
1107	50	0.8880462125236903
1108	1	0.10443173240768999
1108	2	0.21915163853685726
1108	3	0.16474263272144896
1108	4	0.1445776844390636
1108	5	0.14412042638243605
1108	6	0.11970998058308169
1108	7	0.2301796817777202
1108	8	0.243520232891143
1108	9	0.3367142695820533
1108	10	0.17004868884355773
1108	11	0.24266786050686306
1108	12	0.2549351759171543
1108	13	0.33761578681683346
1108	14	0.33092237933845325
1108	15	0.36677190221487166
1108	16	0.35623091050366895
1108	17	0.25105025542426773
1108	18	0.5617192582462778
1108	19	0.34195212793258634
1108	20	0.29259166123112124
1108	21	0.42079022195026994
1108	22	0.4191396919806252
1108	23	0.19481888701159988
1108	24	0.32818015581959514
1108	25	0.3588935786684786
1108	26	0.27890694500388236
1108	27	0.27889771938781394
1108	28	0.34464814147462164
1108	29	0.3394594199103196
1108	30	0.46389688661354
1108	31	0.5247082635771676
1108	32	0.3982185153082156
1108	33	0.4394117285681105
1108	34	0.3618204811638961
1108	35	0.3238416718961119
1108	36	0.33811722570251135
1108	37	0.3119365083524812
1108	38	0.3187296334057379
1108	39	0.44402740943124996
1108	40	0.38476767909139653
1108	41	0.35650916705273517
1108	42	0.32944715521033174
1108	43	0.46527234796253936
1108	44	0.3727825569698311
1108	45	0.3342305652674682
1108	46	0.3646782299198321
1108	47	0.40374107141890647
1108	48	0.3402029715755006
1108	49	0.27641047532354435
1108	50	0.21936431449957894
1109	1	-0.032032943590120766
1109	2	0.0670440449060834
1109	3	-0.013853560942944654
1109	4	0.0663070504560963
1109	5	-0.0012040299093953877
1109	6	0.0035509793538182066
1109	7	-0.10253518243366244
1109	8	-0.045192728869437815
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2210	50	-0.04916446650591766
2211	1	-0.14204710873775994
2211	2	-0.18544224495459005
2211	3	-0.21734020018777755
2211	4	-0.15664686661322946
2211	5	-0.3382122748155538
2211	6	-0.35700017338056966
2211	7	-0.2602882751679303
2211	8	-0.28496211976808894
2211	9	-0.08238918256836314
2211	10	-0.25643114234605235
2211	11	-0.2364467257116445
2211	12	-0.11825218876207799
2211	13	-0.27166835232208797
2211	14	-0.1275434607984849
2211	15	-0.2986036997182282
2211	16	-0.2791855228936626
2211	17	-0.13137209977293196
2211	18	0.19521752036518336
2211	19	-0.08921571951889007
2211	20	-0.08653097891807704
2211	21	-0.1354552084262508
2211	22	-0.16117595138504015
2211	23	0.09946808725628387
2211	24	0.03644010998684166
2211	25	-0.016065650762861594
2211	26	-0.01114452550635292
2211	27	-0.06679167940001207
2211	28	-0.03901483334878719
2211	29	-0.08557592898792166
2211	30	0.021668831512080544
2211	31	-0.05662225363055029
2211	32	-0.08100568294263155
2211	33	-0.16021814420728475
2211	34	-0.265481872430378
2211	35	-0.25835183380705473
2211	36	-0.1668084404459549
2211	37	-0.15223839002066342
2211	38	-0.16734307748054533
2211	39	-0.18584344087918225
2211	40	-0.2756040353468663
2211	41	-0.11259399044168106
2211	42	0.010001548511660019
2211	43	-0.15299840632931705
2211	44	-0.14377923180424546
2211	45	-0.17964246785462024
2211	46	-0.3245499717238758
2211	47	-0.21456244862025642
2211	48	-0.21504770014196595
2211	49	-0.19214874719320463
2211	50	-0.18416931812567583
2212	1	-0.22520455760659175
2212	2	-0.24767899033606255
2212	3	-0.3515645078784324
2212	4	-0.3464850686873575
2212	5	-0.24611304599278583
2212	6	-0.31442950475447873
2212	7	-0.3949345004143012
2212	8	-0.2828613696117728
2212	9	-0.40400438113238124
2212	10	-0.16382840577632152
2212	11	-0.26249569623912095
2212	12	-0.33531397605123914
2212	13	-0.2409846801764104
2212	14	-0.44458447967532777
2212	15	-0.49679692675390186
2212	16	-0.494802043313463
2212	17	-0.14588600056367795
2212	18	0.0192457054363544
2212	19	-0.2668021364722293
2212	20	-0.33036003026560934
2212	21	-0.18792480306029802
2212	22	-0.12647147673907
2212	23	0.1634893221688003
2212	24	-0.026707068302407322
2212	25	-0.2585647188671558
2212	26	-0.2244876465063399
2212	27	-0.14603303581090188
2212	28	-0.24638640454862326
2212	29	-0.17039027421638053
2212	30	-0.035668004675962095
2212	31	-0.10065760033204896
2212	32	-0.1582803527706389
2212	33	-0.1194069514849403
2212	34	-0.3688067753033266
2212	35	-0.27046663578276764
2212	36	-0.3252887860474871
2212	37	-1.4331810360615833
2212	38	-0.866948351394754
2212	39	-0.3642824516588732
2212	40	-0.383242960401617
2212	41	-1.3347954074281567
2212	42	-0.6697122892794667
2212	43	-0.5411379384971582
2212	44	-0.44994129549004924
2212	45	-0.38247592002240793
2212	46	-0.3908155681544869
2212	47	-0.24272802967971785
2212	48	-0.17741260682772264
2212	49	-0.08164232934348092
2212	50	0.020984404422854652
2213	1	-0.9165881294218359
2213	2	-0.975292804791124
2213	3	-0.9907115478929249
2213	4	-0.9401671129665772
2213	5	-0.8699838781159102
2213	6	-0.9995865586037027
2213	7	-0.90789360440019
2213	8	-1.0166647701243186
2213	9	-1.042286366869476
2213	10	-1.1256913061363747
2213	11	-1.0179651706242012
2213	12	-1.0543073583375815
2213	13	-0.9725326106898535
2213	14	-2.769108187215945
2213	15	-1.7726930966687746
2213	16	-1.5077798756114404
2213	17	-1.2577861315165535
2213	18	-1.2181508670565009
2213	19	-1.1441596636633946
2213	20	-1.379427562137245
2213	21	-1.3337301212874166
2213	22	-1.3929841972185315
2213	23	-2.019251012528734
2213	24	-1.6836605757519933
2213	25	-1.4398834778064966
2213	26	-1.6580738631790268
2213	27	-1.4737223528103485
2213	28	-1.2949376565927935
2213	29	-1.7713174709707005
2213	30	-1.5131280370856288
2213	31	-1.3591217076026538
2213	32	-1.3804997271772095
2213	33	-1.5054332250464952
2213	34	-1.4086043577688514
2213	35	-1.4809128039136041
2213	36	-1.334084311789148
2213	37	-1.5801112638963382
2213	38	-1.478837100054509
2213	39	-1.4383637431891074
2213	40	-1.6738289482982864
2213	41	-1.8036857032725244
2213	42	-1.6293456728832503
2213	43	-1.652864832563207
2213	44	-1.6692358645540075
2213	45	-1.4828269386407733
2213	46	-1.5746542134129697
2213	47	-1.535230642359977
2213	48	-1.6156922838160028
2213	49	-1.7638119752379176
2213	50	-1.7029382373153545
2214	1	-0.9860375731686979
2214	2	-0.8795167585624135
2214	3	-1.0296806609596736
2214	4	-1.202533024920271
2214	5	-1.014596064223852
2214	6	-0.8906798959087503
2214	7	-1.088082084878856
2214	8	-1.0455598594286606
2214	9	-1.3240603589445428
2214	10	-1.3854578545868108
2214	11	-1.2542152919254477
2214	12	-1.2233631462608017
2214	13	-1.3330568021519795
2214	14	-1.8701683183794486
2214	15	-1.5076158783421931
2214	16	-1.20448694317637
2214	17	-1.8358642365641913
2214	18	-1.912329146388494
2214	19	-1.5170814981340257
2214	20	-1.3999818148496388
2214	21	-1.5719120419218697
2214	22	-1.4754355052493011
2214	23	-1.1574257087351825
2214	24	-1.1833078186154111
2214	25	-1.2321644824645404
2214	26	-1.5205727316301463
2214	27	-1.2926223943051056
2214	28	-1.3009569403316874
2214	29	-1.1891861359560068
2214	30	-1.1827013372528228
2214	31	-1.114692007843999
2214	32	-1.1630738504841247
2214	33	-1.1864016496864522
2214	34	-1.2764795040754393
2214	35	-1.381440179210557
2214	36	-1.3809624647770988
2214	37	-1.4505583542599467
2214	38	-1.4702881935777903
2214	39	-1.3779946663160274
2214	40	-1.3620252184429376
2214	41	-1.3111940616337172
2214	42	-1.2374276063213898
2214	43	-1.2980984069042023
2214	44	-1.4104944060095477
2214	45	-1.2770096209794914
2214	46	-1.2828779252573206
2214	47	-1.1816167871627883
2214	48	-1.2555518056478832
2214	49	-1.2543565580256129
2214	50	-1.2979927547247563
2215	1	-0.5643378015143483
2215	2	-0.8407744471807348
2215	3	-0.6160786555593702
2215	4	-0.4117679160398424
2215	5	-0.6204629242723954
2215	6	-0.586805836650079
2215	7	-0.5518153613773649
2215	8	-0.6884196712221033
2215	9	-0.5856442150655568
2215	10	-1.0709515537833454
2215	11	-0.876262215327273
2215	12	-0.9696856723095166
2215	13	-0.9049131186637354
2215	14	-0.9169605686766502
2215	15	-0.9161516234487834
2215	16	-0.7896099655532893
2215	17	-0.8964496671014075
2215	18	-0.8460039868469522
2215	19	-1.092876321243125
2215	20	-1.048167126057649
2215	21	-1.0175721144938734
2215	22	-1.4395401909235517
2215	23	-1.3549985759273326
2215	24	-1.1905764531179912
2215	25	-1.1508998558929306
2215	26	-1.072801231522682
2215	27	-1.1719484891700347
2215	28	-1.0611163637970336
2215	29	-1.1304806187436967
2215	30	-1.0456183206510588
2215	31	-1.1028699117929577
2215	32	-1.1869745166890624
2215	33	-1.1682853264250568
2215	34	-1.2977653600069416
2215	35	-1.1606175459987185
2215	36	-1.1200086591109368
2215	37	-1.1280587600450218
2215	38	-1.1062184965908841
2215	39	-1.231544067622373
2215	40	-1.1421480719295616
2215	41	-1.3219330926578188
2215	42	-1.303503316400434
2215	43	-1.3258329574034455
2215	44	-1.1304704953294937
2215	45	-1.2185728336255188
2215	46	-1.1202896352431004
2215	47	-1.2950383489824624
2215	48	-1.1727036457897846
2215	49	-1.277480852011891
2215	50	-1.359243059997688
2216	1	-1.4764942212567043
2216	2	-1.727478042566947
2216	3	-1.5256135018448198
2216	4	-2.7238908591880353
2216	5	-1.8324815913911117
2216	6	-1.7003200528063118
2216	7	-1.6727425063367178
2216	8	-1.6591238050828956
2216	9	-1.6606585672649743
2216	10	-2.068725289044739
2216	11	-1.8429318497771092
2216	12	-1.9972385475215937
2216	13	-2.036768234813273
2216	14	-2.0412902889120446
2216	15	-2.2329537397393437
2216	16	-2.314145246322198
2216	17	-2.0801638026289027
2216	18	-1.9509838795224184
2216	19	-2.14888294818423
2216	20	-2.33493268875646
2216	21	-2.3745583947884157
2216	22	-2.506747231549891
2216	23	-4.2190114508829915
2216	24	-2.944271414391981
2216	25	-2.49401656954554
2216	26	-2.628929228924328
2216	27	-2.6887981849603904
2216	28	-3.0958584444457102
2216	29	-2.8631453698843927
2216	30	-2.5235345949767876
2216	31	-2.296576953131563
2216	32	-2.487311989248937
2216	33	-2.672590630114744
2216	34	-2.816725933126232
2216	35	-2.9956140487630307
2216	36	-2.7917237148886365
2216	37	-2.7396445106971044
2216	38	-2.8762907775435558
2216	39	-2.9574642109876663
2216	40	-2.9966125675085173
2216	41	-3.005981139405389
2216	42	-3.2273952055202564
2216	43	-3.2317633716727343
2216	44	-3.2824396313342135
2216	45	-3.2543286852629514
2216	46	-3.25528108090489
2216	47	-3.2501148251934247
2216	48	-3.317242901013916
2216	49	-3.57100056687678
2216	50	-3.5416209096037328
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	163307.0
3	8050.0
4	16220.0
5	12413.0
6	9941.0
7	8466.0
8	7499.0
9	7011.0
10	6454.0
11	6484.0
12	6419.0
13	6781.0
14	7196.0
15	7559.0
16	8255.0
17	9033.0
18	9958.0
19	11317.0
20	14412.0
21	18763.0
22	24221.0
23	27739.0
24	30187.0
25	31815.0
26	34021.0
27	38953.0
28	44258.0
29	50304.0
30	58231.0
31	68647.0
32	83479.0
33	102479.0
34	129762.0
35	167063.0
36	220315.0
37	299890.0
38	476087.0
39	970161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.39625455880171	17.875563260939174	12.828963912252341	24.899218268006777
2	15.5131645907326	20.167763754944083	39.39914696713483	24.91992468718848
3	18.633341299183265	24.9166787716347	29.370932145015953	27.07904778416608
4	12.48284752354482	16.47968696886685	38.29504384035512	32.74242166723321
5	13.739212050883564	37.694379924525755	34.73324372407096	13.833164300519721
6	31.73000059040231	37.18459943390103	17.806721027412472	13.278678948284195
7	27.95186081602732	32.18198338160557	21.934056776651165	17.93209902571594
8	26.01582462343893	35.00437316873559	20.2818507250089	18.69795148281657
9	26.584892499602507	15.80586486208731	19.27663348157348	38.3326091567367
10	14.182274624668622	29.072225946708535	34.36960136055867	22.37589806806417
11	34.793291315030444	23.220797248257202	23.734156457268586	18.251754979443767
12	23.116978380541582	25.118909676756246	30.551812753615398	21.21229918908677
13	27.742135616125534	22.25697406742476	25.922172597059333	24.078717719390376
14	21.902262436653835	21.423889946523996	27.43002166156107	29.243825955261098
15	23.576451386439324	29.12289603368783	23.821055581609038	23.47959699826381
16	23.38990622905853	28.26533911715041	25.417595446259394	22.927159207531666
17	22.33890548874128	27.825428363157346	26.75234502641309	23.08332112168829
18	22.29346642989874	27.63714648828261	27.534456106500578	22.53493097531807
19	23.245766947828802	27.155857544517335	27.55135895032802	22.047016557325836
20	23.492161687170473	26.815731302479982	27.412739699277484	22.279367311072058
21	23.783753891375177	26.553300748468757	27.261121740836604	22.401823619319465
22	23.277322082137687	26.609348468919926	27.198474152918994	22.914855296023394
23	23.188091121701166	26.638864121595574	27.000230559722777	23.17281419698048
24	22.715300138820687	26.738538546793567	27.367573686795044	23.178587627590705
25	23.234477397811514	26.497804265997495	27.299334656355757	22.968383679835238
26	22.942573887427457	26.75695208444581	27.360149829258013	22.94032419886872
27	23.076502486387046	26.9458368795124	26.985391616895182	22.992269017205373
28	23.01468258357973	26.741170169350003	27.24365164034576	23.000495606724503
29	23.059956198581844	26.92952933629509	27.18312636137326	22.827388103749804
30	23.41484675227294	26.77714250007811	27.072421657762362	22.735589089886588
31	23.44248108320889	26.62887473991365	26.996682141674427	22.93196203520304
32	23.06785766730091	26.882090312340857	26.996566343921742	23.053485676436498
33	22.998846503769173	26.742522894963106	27.037301793605405	23.221328807662314
34	23.173352334832064	26.787083770806184	27.186721276190973	22.852842618170776
35	23.430531978944927	26.65974912507471	27.022467012793705	22.887251883186654
36	23.305382643033774	26.816727169558284	26.970926740986588	22.90696344642135
37	23.380968987451737	26.50134807276218	27.00626600719222	23.111416932593862
38	23.176235455920438	26.72808237945375	27.104531163641965	22.991151000983848
39	23.32324806145373	27.013045672974805	26.79857485924767	22.865131406323798
40	23.432309109555387	26.660546085384297	27.185314603396645	22.72183020166368
41	23.32312001991643	26.70225968387978	26.975985646176632	22.99863465002716
42	23.323485016981003	26.798655764170203	26.811744507725795	23.066114711122996
43	23.15573450497174	26.528327885522902	27.14964686570278	23.16629074380258
44	23.239829428089397	26.823444830530548	26.843682599329032	23.093043142051023
45	23.384317861616875	26.801404575708638	26.762676614339153	23.051600948335338
46	23.38148864629394	26.619854322266228	26.592276619042295	23.40638041239754
47	23.27979176718397	26.850728486955	26.66619823651663	23.2032815093444
48	23.238605201447722	26.87998715691921	26.814147627965433	23.06726001366764
49	23.344816373905875	26.72682118222739	26.66241453703585	23.265947906830885
50	23.367293079270294	26.884719051292542	26.681023039949032	23.06696482948814
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1738.0
1	1425.5
2	1113.0
3	1100.5
4	1088.0
5	1045.0
6	1002.0
7	978.5
8	955.0
9	1103.5
10	1252.0
11	1594.0
12	1936.0
13	3293.5
14	4651.0
15	6716.5
16	8782.0
17	10162.5
18	11543.0
19	12757.5
20	13972.0
21	16815.5
22	19659.0
23	24810.5
24	29962.0
25	37511.5
26	45061.0
27	53455.0
28	61849.0
29	69994.0
30	78139.0
31	87463.0
32	96787.0
33	107347.0
34	117907.0
35	128435.5
36	138964.0
37	148136.5
38	157309.0
39	166676.5
40	176044.0
41	184357.5
42	192671.0
43	198837.0
44	205003.0
45	211402.5
46	217802.0
47	221516.0
48	225230.0
49	225201.0
50	225172.0
51	221669.0
52	218166.0
53	211159.0
54	204152.0
55	193926.0
56	183700.0
57	170509.0
58	157318.0
59	142497.5
60	127677.0
61	110855.0
62	94033.0
63	79897.0
64	65761.0
65	54871.5
66	43982.0
67	35691.5
68	27401.0
69	22789.5
70	18178.0
71	14285.5
72	10393.0
73	8658.0
74	6923.0
75	5496.5
76	4070.0
77	3254.0
78	2438.0
79	1956.5
80	1475.0
81	1138.5
82	802.0
83	632.5
84	463.0
85	354.5
86	246.0
87	195.0
88	144.0
89	126.0
90	108.0
91	72.5
92	37.0
93	34.5
94	32.0
95	28.0
96	24.0
97	16.0
98	8.0
99	18.0
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060097091925136195
2	0.06559168318686293
3	0.061970248037088484
4	0.07636233083058865
5	0.08079546696220907
6	0.060659038758721875
7	0.04735963036386057
8	0.05650687604389429
9	0.0566005338494919
10	0.06593509514072085
11	0.05269812528292462
12	0.06840141735479138
13	0.06371852707491063
14	0.06518583269593994
15	0.07708037400683702
16	0.0707116432261992
17	0.08282471941682407
18	0.07074286249473175
19	0.06712142734495731
20	0.07882865304465916
21	0.07748622449776002
22	0.07196041396750075
23	0.07005603858701591
24	0.05925417167475766
25	0.0748638059410268
26	0.08451055991758114
27	0.07873499523906155
28	0.09465682219065606
29	0.08732029408550958
30	0.0764872079047188
31	0.07149212493951267
32	0.07742378596069494
33	0.07636233083058865
34	0.06240731779654403
35	0.08088912476780669
36	0.08591542700154536
37	0.09446950657946085
38	0.07645598863618626
39	0.055632736524983226
40	0.060221968999266344
41	0.05535176310819038
42	0.06012831119366873
43	0.039086524202737935
44	0.03796263053556655
45	0.041490407879743375
46	0.04021041786990931
47	0.030126594133899443
48	0.044518676927399595
49	0.050481557217114406
50	0.041209434462950535
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3203150.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.64281647130443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.28312785574514	48.412327945957976
2	12.673189256572398	15.877685374111433
3	4.523993409038852	8.50187066519435
4	1.9785709039592845	4.957730160487374
5	1.1100125455581793	3.476715608612324
6	0.6299880701074335	2.3678536232910736
7	0.40966812496707206	1.796393561651899
8	0.27919544307251026	1.3991671120012628
9	0.20701944662132551	1.167145308062163
>10	0.8558751498810114	9.043951956410957
>50	0.03674944597974926	1.555246704242492
>100	0.01236004236698744	1.21859209054707
>500	1.0012245200781406E-4	0.046537886701512245
>1k	1.5018367798762743E-4	0.17878200272804803
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.121926853253828E-5
2	0.0	0.0	0.0	0.0	3.121926853253828E-5
3	0.0	0.0	0.0	0.0	6.243853706507656E-5
4	0.0	0.0	0.0	0.0	6.243853706507656E-5
5	0.0	0.0	0.0	0.0	6.243853706507656E-5
6	0.0	0.0	0.0	0.0	6.243853706507656E-5
7	0.0	0.0	0.0	0.0	6.243853706507656E-5
8	0.0	0.0	0.0	3.121926853253828E-5	6.243853706507656E-5
9	0.0	0.0	0.0	3.121926853253828E-5	6.243853706507656E-5
10	0.0	0.0	0.0	3.121926853253828E-5	6.243853706507656E-5
11	0.0	0.0	0.0	3.121926853253828E-5	1.2487707413015312E-4
12	0.0	0.0	0.0	3.121926853253828E-5	1.873156111952297E-4
13	0.0	0.0	0.0	3.121926853253828E-5	2.1853487972776797E-4
14	0.0	0.0	0.0	3.121926853253828E-5	2.1853487972776797E-4
15	0.0	0.0	0.0	3.121926853253828E-5	2.1853487972776797E-4
16	0.0	3.121926853253828E-5	0.0	6.243853706507656E-5	2.4975414826030625E-4
17	0.0	3.121926853253828E-5	0.0	6.243853706507656E-5	2.4975414826030625E-4
18	0.0	3.121926853253828E-5	0.0	6.243853706507656E-5	2.4975414826030625E-4
19	0.0	3.121926853253828E-5	0.0	6.243853706507656E-5	2.4975414826030625E-4
20	0.0	3.121926853253828E-5	0.0	9.365780559761485E-5	2.8097341679284455E-4
21	0.0	3.121926853253828E-5	0.0	1.5609634266269143E-4	2.8097341679284455E-4
22	0.0	3.121926853253828E-5	0.0	2.4975414826030625E-4	2.8097341679284455E-4
23	0.0	3.121926853253828E-5	0.0	2.8097341679284455E-4	2.8097341679284455E-4
24	0.0	3.121926853253828E-5	0.0	3.1219268532538285E-4	2.8097341679284455E-4
25	0.0	3.121926853253828E-5	0.0	3.434119538579211E-4	2.8097341679284455E-4
26	0.0	3.121926853253828E-5	0.0	3.746312223904594E-4	2.8097341679284455E-4
27	0.0	3.121926853253828E-5	0.0	3.746312223904594E-4	2.8097341679284455E-4
28	0.0	3.121926853253828E-5	0.0	6.243853706507657E-4	2.8097341679284455E-4
29	0.0	3.121926853253828E-5	0.0	0.00109267439863884	2.8097341679284455E-4
30	0.0	3.121926853253828E-5	0.0	0.001779498306354682	2.8097341679284455E-4
31	0.0	3.121926853253828E-5	0.0	0.003871189298034747	2.8097341679284455E-4
32	0.0	3.121926853253828E-5	0.0	0.0060253188267798885	2.8097341679284455E-4
33	0.0	3.121926853253828E-5	0.0	0.00874139518911072	2.8097341679284455E-4
34	0.0	3.121926853253828E-5	0.0	0.012113076190624853	3.1219268532538285E-4
35	0.0	3.121926853253828E-5	0.0	0.016077923294257217	3.1219268532538285E-4
36	0.0	3.121926853253828E-5	0.0	0.020792032842670498	3.1219268532538285E-4
37	0.0	3.121926853253828E-5	0.0	0.027004667280645614	3.1219268532538285E-4
38	0.0	3.121926853253828E-5	0.0	0.03324852098715327	3.1219268532538285E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4200	0.0	24.200611	1
GGTATCA	1870	0.0	23.177055	1
TATCAAC	5535	0.0	18.04506	2
ATCAACG	5450	0.0	18.043928	3
TCAACGC	5470	0.0	17.858412	4
CAACGCA	5590	0.0	17.474228	5
AACGCAG	5785	0.0	16.99744	6
GTACATG	7620	0.0	14.609292	1
ACGCAGA	6760	0.0	14.319216	7
TACATGG	7640	0.0	14.282635	2
TATACCG	110	0.0010185691	14.000134	5
CGCAGAG	6920	0.0	13.82918	8
GTATACG	145	3.8387636E-5	13.655517	1
GTATTAT	790	0.0	13.645914	1
TTAGACA	775	0.0	13.626575	4
GTATAGG	605	0.0	13.454885	1
GTATTAG	645	0.0	13.302661	1
TAATGCG	100	0.008764071	13.200127	5
ACATGGG	7780	0.0	13.149021	3
AGAGTAC	6895	0.0	12.987159	11
>>END_MODULE