FastQCFastQC Report
Mon 6 Feb 2017
SRR2077414_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077414_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3203150
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33620.1049591808063937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21050.028.538731
GTATCAA50250.024.610721
ATCAACG72950.016.8001543
TCAACGC73350.016.7390584
TATCAAC73450.016.6857912
CAACGCA75200.016.3570335
CGCGCAT953.346896E-416.21152710
AACGCAG77750.015.7327116
TCTATAC6000.014.6687173
GTACATG73200.014.4596611
TACATGG74050.013.8763492
TATACTG9650.013.45355
ACGCAGA90500.013.3701557
ACATGGG75850.013.2279533
CGCAGAG92900.013.0012698
ACGCCCG2054.4233275E-712.87884321
GGCGTGC4450.012.8541248
TAGGACA8450.012.7595794
CTATACT7950.012.7317374
GTACCGA1953.5524936E-612.4104416
Produced by FastQC (version 0.11.5)