##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077414_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3203150 Sequences flagged as poor quality 0 Sequence length 50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.48292586984687 31.0 31.0 34.0 27.0 34.0 2 30.43831759361878 31.0 31.0 34.0 26.0 34.0 3 30.632599160201675 31.0 31.0 34.0 27.0 34.0 4 34.1094457018872 37.0 35.0 37.0 30.0 37.0 5 33.73391317921421 37.0 35.0 37.0 28.0 37.0 6 33.67736228400169 37.0 35.0 37.0 28.0 37.0 7 33.50762530633907 37.0 35.0 37.0 28.0 37.0 8 33.4767981518193 37.0 35.0 37.0 27.0 37.0 9 34.93146933487348 39.0 35.0 39.0 27.0 39.0 10 34.79255170691351 39.0 35.0 39.0 27.0 39.0 11 34.74548616205922 39.0 35.0 39.0 27.0 39.0 12 34.66940948753571 39.0 35.0 39.0 27.0 39.0 13 34.63528495387353 39.0 35.0 39.0 27.0 39.0 14 35.78854003090708 40.0 36.0 41.0 27.0 41.0 15 35.79852426517647 40.0 36.0 41.0 27.0 41.0 16 35.734805425908874 40.0 36.0 41.0 27.0 41.0 17 35.71125454630598 40.0 36.0 41.0 27.0 41.0 18 35.70725286046548 40.0 36.0 41.0 27.0 41.0 19 35.74528261242839 40.0 36.0 41.0 27.0 41.0 20 35.73493717122208 40.0 36.0 41.0 27.0 41.0 21 35.679248864399106 40.0 36.0 41.0 27.0 41.0 22 35.68062719510482 40.0 36.0 41.0 27.0 41.0 23 35.61686870736619 40.0 36.0 41.0 26.0 41.0 24 35.60268017420352 40.0 36.0 41.0 26.0 41.0 25 35.58161622153193 40.0 36.0 41.0 26.0 41.0 26 35.50278194901893 40.0 35.0 41.0 26.0 41.0 27 35.44443438490236 40.0 35.0 41.0 25.0 41.0 28 35.38527074910635 40.0 35.0 41.0 25.0 41.0 29 35.30529759767729 40.0 35.0 41.0 25.0 41.0 30 35.18461608104522 39.0 35.0 41.0 24.0 41.0 31 35.09518068151663 39.0 35.0 41.0 24.0 41.0 32 35.01991539578228 39.0 35.0 41.0 24.0 41.0 33 34.92038867989323 39.0 35.0 41.0 24.0 41.0 34 34.89732763061362 39.0 35.0 41.0 24.0 41.0 35 34.834091753430215 39.0 35.0 41.0 24.0 41.0 36 34.72301422037682 39.0 34.0 41.0 23.0 41.0 37 34.63341710503723 39.0 34.0 41.0 22.0 41.0 38 34.55282737305465 39.0 34.0 41.0 22.0 41.0 39 34.4603702605248 39.0 34.0 41.0 20.0 41.0 40 34.371473393378395 39.0 34.0 41.0 19.0 41.0 41 34.313467992444934 39.0 34.0 41.0 19.0 41.0 42 34.19548756692631 39.0 34.0 41.0 18.0 41.0 43 34.29776938326335 39.0 34.0 41.0 18.0 41.0 44 34.23124486833274 39.0 34.0 41.0 16.0 41.0 45 34.1630635468211 39.0 34.0 41.0 15.0 41.0 46 34.01319700919407 39.0 34.0 41.0 9.0 41.0 47 33.871972277289544 39.0 34.0 41.0 7.0 41.0 48 33.71613037166539 39.0 33.0 41.0 2.0 41.0 49 33.56426423988886 39.0 33.0 41.0 2.0 41.0 50 33.42055976148479 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8093249910615441 1101 2 0.9457945741194074 1101 3 0.8491449191548632 1101 4 0.9029485652576454 1101 5 0.9272209208251496 1101 6 0.9557501013707537 1101 7 0.9386877825179027 1101 8 0.8839947237939683 1101 9 1.033853329406348 1101 10 1.074458303530072 1101 11 1.133419309380841 1101 12 1.1591314174200917 1101 13 1.1454916483449438 1101 14 1.2701049212355713 1101 15 1.22622085787485 1101 16 1.2501112261816445 1101 17 1.3465330587734456 1101 18 1.260111359524096 1101 19 1.2563970595564626 1101 20 1.1900165351084624 1101 21 1.2892070444641064 1101 22 1.229906780904173 1101 23 1.3928313201653992 1101 24 1.258964788280494 1101 25 1.3160844874395679 1101 26 1.265628522411447 1101 27 1.3557673377852097 1101 28 1.3845470433215183 1101 29 1.3798519069993915 1101 30 1.383604267780548 1101 31 1.460719149727126 1101 32 1.3988576321911808 1101 33 1.4683900389005373 1101 34 1.4266000536584613 1101 35 1.3933287956659584 1101 36 1.37147673690815 1101 37 1.4834598882511827 1101 38 1.4572526421990233 1101 39 1.4398251311456463 1101 40 1.5213823407732079 1101 41 1.4069914003678576 1101 42 1.4367381312006273 1101 43 1.47299414168711 1101 44 1.5757190627938726 1101 45 1.4664803186727653 1101 46 1.4557631560646485 1101 47 1.4956897787915935 1101 48 1.572382580616221 1101 49 1.60060824044578 1101 50 1.6627124557879043 1102 1 0.5667391152347889 1102 2 0.729024785341938 1102 3 0.6304549255555436 1102 4 0.6539620066914011 1102 5 0.6195614371468849 1102 6 0.637422813126669 1102 7 0.6833005412121622 1102 8 0.6941078025223675 1102 9 0.7462493183118326 1102 10 0.7589046444731053 1102 11 0.7687937493211052 1102 12 0.8258692027838634 1102 13 0.8794366024733833 1102 14 0.9795139248626228 1102 15 0.8831442630380693 1102 16 1.0369684909565535 1102 17 0.9447835388246517 1102 18 0.9959765184743858 1102 19 1.0182916616473534 1102 20 0.9860481118099784 1102 21 1.0613427389381869 1102 22 0.9527572182841553 1102 23 0.9112866220642744 1102 24 0.9344501733882424 1102 25 0.8200315751289224 1102 26 0.8510776369836677 1102 27 0.7459956288029232 1102 28 0.8867872822803378 1102 29 0.9047078916377487 1102 30 0.9950828254933768 1102 31 0.9260487848882093 1102 32 0.9260605338340255 1102 33 0.9836449994296572 1102 34 0.9004639324722064 1102 35 0.9469878526320343 1102 36 0.9355902423487308 1102 37 0.8512857896806665 1102 38 0.9072206387853257 1102 39 0.910275312498328 1102 40 0.9254787777264823 1102 41 0.9593703207860358 1102 42 0.8500088800805088 1102 43 0.8909587245759525 1102 44 0.9674408464507991 1102 45 0.8765507261828986 1102 46 1.0375852170844908 1102 47 1.0435882212921257 1102 48 1.067582068561606 1102 49 1.0938875235693075 1102 50 1.151511260993793 1103 1 0.4828278070904126 1103 2 0.7310430102165846 1103 3 0.5534191765917562 1103 4 0.5640968928784105 1103 5 0.6380238390450401 1103 6 0.6611028019589753 1103 7 0.6986887992199939 1103 8 0.6687425988131466 1103 9 0.8249976583253229 1103 10 0.8047315564617392 1103 11 0.7895271271655702 1103 12 0.8123131897321514 1103 13 0.9040869097030395 1103 14 0.9492871580077704 1103 15 0.9218860879675788 1103 16 1.0599170240633171 1103 17 0.9730536883221461 1103 18 0.895668663395746 1103 19 0.9366268021790418 1103 20 0.9613409155578552 1103 21 0.9941331664120057 1103 22 1.0170761340488355 1103 23 1.0689108795078397 1103 24 0.9247396891019619 1103 25 0.9475336738575493 1103 26 0.8797265569758466 1103 27 0.8577886118758542 1103 28 0.9261251510368282 1103 29 0.9835664930821224 1103 30 1.0425378852800407 1103 31 1.1053851962529961 1103 32 1.0295531948607817 1103 33 1.0156605209628893 1103 34 1.0004075286534473 1103 35 1.0289781547007237 1103 36 0.9663745038181588 1103 37 1.059757927258083 1103 38 1.002391076827685 1103 39 1.0395152417725413 1103 40 0.9716737768252912 1103 41 1.0128163555165628 1103 42 0.9109696074694256 1103 43 0.9075951134922846 1103 44 0.9391588116880456 1103 45 0.974705567685767 1103 46 1.03271657619662 1103 47 0.9532440467124488 1103 48 1.0278176656788887 1103 49 1.0714408869636074 1103 50 1.0956214910867388 1104 1 0.493435515785535 1104 2 0.6983245080937976 1104 3 0.6054149637786423 1104 4 0.6104450895575724 1104 5 0.6633398291677182 1104 6 0.6328136505398376 1104 7 0.6585968812568339 1104 8 0.6997529318782725 1104 9 0.6510554192100599 1104 10 0.7401004813055394 1104 11 0.7660273158804998 1104 12 0.7149450927906571 1104 13 0.7522523126509668 1104 14 0.8744226958198453 1104 15 0.741052505439022 1104 16 0.84237643438086 1104 17 0.8536936432464088 1104 18 0.8473887234581596 1104 19 0.7835195719085348 1104 20 0.7959447883948556 1104 21 0.7949435333132868 1104 22 0.8229401240069478 1104 23 0.8157796859164534 1104 24 0.8189135457362084 1104 25 0.7863932701660303 1104 26 0.818170781860843 1104 27 0.8262150911252633 1104 28 0.888504593538002 1104 29 0.7613489763219334 1104 30 0.8926502702762846 1104 31 0.8473712871662329 1104 32 0.9222187998198024 1104 33 0.9185730249684667 1104 34 0.9456648308721896 1104 35 0.9384557279722188 1104 36 1.0151379839416208 1104 37 0.945875687532407 1104 38 0.9082204060934274 1104 39 0.9358566327994993 1104 40 0.9464240948648808 1104 41 0.9066907461862073 1104 42 0.9624609604922227 1104 43 0.9230724141923261 1104 44 0.9185686163772644 1104 45 0.8963841018298737 1104 46 0.9340810695312456 1104 47 1.0236176850046164 1104 48 0.9751666105265144 1104 49 1.0511122980742584 1104 50 1.1575654884043658 1105 1 0.47559258988365016 1105 2 0.6018889110341661 1105 3 0.4894517346423477 1105 4 0.5013927157215292 1105 5 0.5788659888249796 1105 6 0.5438844123475519 1105 7 0.611702287589388 1105 8 0.5748541437796746 1105 9 0.5066262898914786 1105 10 0.634675354068932 1105 11 0.7010216757827408 1105 12 0.6169618129459735 1105 13 0.6202920154850347 1105 14 0.6556706745449432 1105 15 0.6898534216712946 1105 16 0.6517209385280438 1105 17 0.6996168312443629 1105 18 0.6628177518611551 1105 19 0.6786895672657423 1105 20 0.7273538510888358 1105 21 0.7893207063425436 1105 22 0.70437340813546 1105 23 0.7245144465619475 1105 24 0.663623249176446 1105 25 0.7046674747530872 1105 26 0.6513744149574947 1105 27 0.6725824516374033 1105 28 0.7148275105538957 1105 29 0.7366718554098171 1105 30 0.7576123016325838 1105 31 0.7474258256077917 1105 32 0.8014641120705832 1105 33 0.8120687274205451 1105 34 0.7479050299535857 1105 35 0.7070314771434738 1105 36 0.7786393383439574 1105 37 0.7357382763965461 1105 38 0.7345307509910768 1105 39 0.7986790335542651 1105 40 0.8388842272810351 1105 41 0.7911716049033686 1105 42 0.7653910598534424 1105 43 0.6964206579732348 1105 44 0.8071586105627659 1105 45 0.7345504621940222 1105 46 0.7224055422726963 1105 47 0.7738557509742918 1105 48 0.708725285321961 1105 49 0.7226859429184387 1105 50 0.8411751797550551 1106 1 0.4291681097530642 1106 2 0.5557708699509547 1106 3 0.4317810077480537 1106 4 0.4555330228862857 1106 5 0.5116592830538522 1106 6 0.4682027490835594 1106 7 0.4827261601770161 1106 8 0.49967724751098785 1106 9 0.5547872165353169 1106 10 0.6313014593288813 1106 11 0.5503294665088134 1106 12 0.44764077716045136 1106 13 0.44584017603887816 1106 14 0.4936295750956532 1106 15 0.5628284303616056 1106 16 0.563092991817129 1106 17 0.6249336423297862 1106 18 0.5339598424862615 1106 19 0.6123242226638936 1106 20 0.5606196943769532 1106 21 0.6058572475627813 1106 22 0.6016486024951959 1106 23 0.6693161915165149 1106 24 0.6238749815221922 1106 25 0.6658594008670562 1106 26 0.710416661627093 1106 27 0.5948566187345747 1106 28 0.5927694993805943 1106 29 0.605682914719651 1106 30 0.7355962011146104 1106 31 0.6833500752670574 1106 32 0.6631476413073045 1106 33 0.7738773424419776 1106 34 0.7738159467390275 1106 35 0.7061989802559125 1106 36 0.6426166040295129 1106 37 0.6796639900706367 1106 38 0.7100114435913767 1106 39 0.7023549849070889 1106 40 0.8436082266858875 1106 41 0.7193554312177923 1106 42 0.7861630957121974 1106 43 0.7625812542361885 1106 44 0.7040767483412083 1106 45 0.6283852797008294 1106 46 0.6420454436687422 1106 47 0.6059414849815496 1106 48 0.5704703674681753 1106 49 0.6615491161854905 1106 50 0.6446030637698215 1107 1 0.37068234211288953 1107 2 0.5558711451543097 1107 3 0.42981863631424133 1107 4 0.4194939922644494 1107 5 0.4636933849554339 1107 6 0.41975561169962816 1107 7 0.5134894080528696 1107 8 0.42157274865888184 1107 9 0.48914580221463666 1107 10 0.6578717332737867 1107 11 0.5566129027239626 1107 12 0.6320876397372288 1107 13 0.677226290181963 1107 14 0.577013246853376 1107 15 0.631615422670393 1107 16 0.7611814891157991 1107 17 0.612078279569019 1107 18 0.6554262801306265 1107 19 0.5321955534440974 1107 20 0.5307631712421568 1107 21 0.5834810912171093 1107 22 0.651539866188628 1107 23 0.7377891243391161 1107 24 0.5854060642007113 1107 25 0.6046961831840534 1107 26 0.722848172744186 1107 27 0.6494491352308671 1107 28 0.6504215736701795 1107 29 0.7307509272442871 1107 30 0.8007246801995578 1107 31 0.8338655245308075 1107 32 0.744226739735609 1107 33 0.8313115479190571 1107 34 0.7504492919488115 1107 35 0.7544917632636796 1107 36 0.7119389556419193 1107 37 0.7282044181121847 1107 38 0.7692016292181307 1107 39 0.7427376677593287 1107 40 0.5862128079025339 1107 41 0.6347427653281912 1107 42 0.6040937970100089 1107 43 0.6722082578460515 1107 44 0.7436976664103199 1107 45 0.7667444334565374 1107 46 0.7583183201493426 1107 47 0.6966321352439806 1107 48 0.7841225974600334 1107 49 0.7745441576073162 1107 50 0.8711387172412657 1108 1 0.27526148199369516 1108 2 0.3880105328554393 1108 3 0.2513568033673472 1108 4 0.2659231728868079 1108 5 0.24020848603839084 1108 6 0.2270680877600455 1108 7 0.1570903338886751 1108 8 0.18384593092506663 1108 9 0.16381779074428238 1108 10 0.24069193080404716 1108 11 0.18504206756364994 1108 12 0.1680235086714461 1108 13 0.2754971973153033 1108 14 0.2853080728281725 1108 15 0.29543678386917804 1108 16 0.34954719142749724 1108 17 0.3635126512740072 1108 18 0.2821044991734709 1108 19 0.2790455041434825 1108 20 0.17640099251310914 1108 21 0.32519018055896964 1108 22 0.25650545895239674 1108 23 0.3101869715017429 1108 24 0.2144493773153613 1108 25 0.30091390635558213 1108 26 0.28189396050083815 1108 27 0.2595428418095551 1108 28 0.323249174892247 1108 29 0.32713904256867465 1108 30 0.4096486479875239 1108 31 0.35234409925032395 1108 32 0.30117080169756605 1108 33 0.31590634501966264 1108 34 0.3231070271070777 1108 35 0.28771928524864165 1108 36 0.43021296265387576 1108 37 0.36365246084842795 1108 38 0.3332217913790174 1108 39 0.40009420377852223 1108 40 0.4683734313909085 1108 41 0.3338706513060785 1108 42 0.35861594728168456 1108 43 0.26808630959257584 1108 44 0.23306862374469972 1108 45 0.16103802635951325 1108 46 0.25422391063473526 1108 47 0.19167048814816923 1108 48 0.18829505026327098 1108 49 0.26345879338472855 1108 50 0.39138911944646537 1109 1 0.20118014345863244 1109 2 0.38763936374843766 1109 3 0.19244241126331474 1109 4 0.3242051275673674 1109 5 0.22558132417153587 1109 6 0.24779205473642918 1109 7 0.20140568661883407 1109 8 0.18595926058840462 1109 9 0.22663551317288722 1109 10 0.2352172462260853 1109 11 0.2742092912362537 1109 12 0.3329598187932348 1109 13 0.3219560735468576 1109 14 0.2286296579170397 1109 15 0.28009500794569675 1109 16 0.377680120795425 1109 17 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45 -1.8010091738422247 2216 46 -1.7604856243975817 2216 47 -1.8691446605114237 2216 48 -1.9725456950350093 2216 49 -1.8972844950818022 2216 50 -1.8955148817821694 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 80986.0 3 12902.0 4 21783.0 5 12822.0 6 9661.0 7 7449.0 8 6266.0 9 5730.0 10 5393.0 11 5223.0 12 5402.0 13 5855.0 14 6134.0 15 6629.0 16 7252.0 17 7933.0 18 8647.0 19 9595.0 20 11335.0 21 14048.0 22 17481.0 23 21595.0 24 25504.0 25 28538.0 26 31275.0 27 35914.0 28 41652.0 29 48331.0 30 55638.0 31 66052.0 32 80918.0 33 100005.0 34 126221.0 35 160923.0 36 209073.0 37 284158.0 38 450874.0 39 1167953.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.88237286960714 18.700183629927462 14.004802917096129 26.412640583369267 2 14.532633559247849 20.327592176275136 38.44184857993543 26.697925684541584 3 17.494370366069035 24.6097968274246 30.03350775598561 27.862325050520752 4 11.992886850851773 16.79554254308819 37.56272864697189 33.64884195908815 5 13.426911601704372 37.78916968546321 34.85776963118265 13.926149081649767 6 31.836312417031536 37.53795353360162 17.37098452941678 13.25474951995006 7 27.546664609721127 32.23743730401895 21.999126214532133 18.216771871727794 8 26.163146899659317 34.92023145213419 20.10398483632537 18.812636811881127 9 26.24440804523218 15.195127016421806 19.366612067848273 39.19385287049774 10 13.854672745573154 29.1408386530011 34.49027844443868 22.514210156987062 11 35.0597088203401 22.762902452065543 23.727338257119584 18.45005047047477 12 23.105889304756715 24.93488184412119 30.733520890279188 21.225707960842907 13 27.86361638994372 21.861378371043298 26.19297244575865 24.08203279325434 14 21.79765230297185 20.971766919359755 27.848231731389077 29.382349046279316 15 23.468666885702184 28.919809264203057 24.131520002398563 23.480003847696192 16 23.224013677554282 28.074498195045074 25.651098683154355 23.05038944424629 17 22.08667156202398 27.580955331790598 27.154178456075933 23.17819465010949 18 21.991276141512042 27.288841550929 28.115374824499895 22.604507483059066 19 22.989115733802446 26.80385643988353 28.25255145865909 21.954476367654934 20 23.23013811072721 26.318998361461677 28.13657026380904 22.314293264002067 21 23.412165570499084 26.14513514923278 27.993861836345413 22.448837443922727 22 22.930879283774676 26.238430271164397 27.969555926056184 22.861134519004743 23 22.79173839930237 26.19158423139678 27.741914930299615 23.274762439001233 24 22.467772674376114 26.357164407615002 28.043004747975036 23.13205817003385 25 22.81064100974471 26.196538926535656 28.082205257665986 22.910614806053648 26 22.64133794707317 26.405852315974336 28.08069109899895 22.87211863795354 27 22.803209868600025 26.57255358083749 27.763370730578362 22.860865819984127 28 22.6942767942252 26.402754474709628 27.982036197455656 22.920932533609513 29 22.765113574928158 26.533391925247212 27.893967375297763 22.807527124526867 30 23.024966481184958 26.461671746410687 27.792524078423874 22.72083769398048 31 22.94165117627071 26.30597154764046 27.84314350102936 22.909233775059462 32 22.7676450904603 26.545701086435468 27.69425159495747 22.992402228146762 33 22.755317315186762 26.376044227271816 27.749496622499347 23.119141835042072 34 22.887079524430323 26.47970710153865 27.86800833730162 22.765205036729405 35 23.1202528562489 26.284484513741347 27.780775480787845 22.81448714922191 36 23.027068921046535 26.436523901626835 27.674775747575737 22.861631429750894 37 23.130432936267184 26.12302670384094 27.661664542853515 23.084875817038363 38 22.914895915755867 26.36713419694976 27.74224653099358 22.975723356300794 39 23.083849872795785 26.69774229686979 27.35892946129607 22.859478369038357 40 23.18662163668397 26.330488310136147 27.708082324066634 22.774807729113245 41 23.08302327781422 26.34435878999201 27.5549412551855 23.017676677008268 42 23.113929907588115 26.513147439714036 27.343134111346973 23.029788541350875 43 22.873219403016297 26.25581902856292 27.67945367396082 23.19150789445997 44 23.026036037217352 26.464788565298647 27.381141934085996 23.128033463398005 45 23.127910505355516 26.547274287148632 27.247762446566586 23.077052760929266 46 23.180663341156958 26.406738683994785 27.06478500011551 23.347812974732747 47 23.14359561442971 26.608694403399664 26.98345155583223 23.264258426338397 48 23.064034241507525 26.6769066506823 27.061748976782084 23.197310131028086 49 23.125327412487596 26.54209330451999 26.97257633588978 23.36000294710263 50 23.25155214834439 26.62399188796247 26.952107322156593 23.17234864153654 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 508.0 1 372.5 2 237.0 3 348.5 4 460.0 5 577.0 6 694.0 7 704.0 8 714.0 9 959.0 10 1204.0 11 1791.0 12 2378.0 13 4342.5 14 6307.0 15 9013.5 16 11720.0 17 14028.5 18 16337.0 19 17703.5 20 19070.0 21 21412.0 22 23754.0 23 27976.0 24 32198.0 25 39171.0 26 46144.0 27 53873.5 28 61603.0 29 69802.5 30 78002.0 31 86820.5 32 95639.0 33 105709.5 34 115780.0 35 126749.0 36 137718.0 37 147051.5 38 156385.0 39 165540.5 40 174696.0 41 183338.0 42 191980.0 43 197602.5 44 203225.0 45 210232.0 46 217239.0 47 221438.5 48 225638.0 49 225317.0 50 224996.0 51 221665.5 52 218335.0 53 211570.0 54 204805.0 55 194805.0 56 184805.0 57 170972.5 58 157140.0 59 141386.5 60 125633.0 61 109164.0 62 92695.0 63 78421.0 64 64147.0 65 53495.5 66 42844.0 67 34490.5 68 26137.0 69 21568.5 70 17000.0 71 13434.5 72 9869.0 73 8271.0 74 6673.0 75 5164.5 76 3656.0 77 2891.0 78 2126.0 79 1666.5 80 1207.0 81 954.0 82 701.0 83 542.0 84 383.0 85 289.0 86 195.0 87 151.0 88 107.0 89 69.5 90 32.0 91 27.0 92 22.0 93 14.5 94 7.0 95 4.5 96 2.0 97 2.0 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01601548475719214 2 6.556046391833039E-4 3 3.434119538579211E-4 4 0.002122910260212603 5 0.039554813230726 6 0.027441737040101152 7 0.030938295115745435 8 0.023726644084729093 9 0.03137536487520098 10 0.032436820005307274 11 0.03989822518458393 12 0.03989822518458393 13 0.03780653419290386 14 0.042895274963707604 15 0.03852457736915224 16 0.0439567300938139 17 0.04611085962255904 18 0.049482540624073174 19 0.051449354541623096 20 0.04732841109532803 21 0.048108892808641494 22 0.0460796403540265 23 0.04514306229805035 24 0.042583082278382214 25 0.04092846104615769 26 0.043987949362346436 27 0.04367575667702106 28 0.04302015203783775 29 0.041927477639198914 30 0.03799384980409909 31 0.03640166710893964 32 0.03206218878291681 33 0.024413467992444936 34 0.02107300625946334 35 0.02179104943571172 36 0.022852504565818024 37 0.01801351794327459 38 0.020230086009084807 39 0.013299408394861309 40 0.007711159327536956 41 0.007149212493951267 42 0.006462388586235425 43 0.002528760751135601 44 0.0039960663721649 45 0.003059488316188752 46 9.99016593041225E-4 47 2.8097341679284455E-4 48 6.243853706507657E-4 49 1.2487707413015312E-4 50 2.8097341679284455E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3203150.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.6288392473234 #Duplication Level Percentage of deduplicated Percentage of total 1 75.49741374763794 45.773205616942036 2 13.643369930843773 16.54363364657786 3 4.821656510959184 8.769943125262635 4 2.234578888059512 5.419196967584912 5 1.2035732175320555 3.6485623564067393 6 0.68734990057946 2.500393597733748 7 0.4179217743689285 1.7736678453318948 8 0.2958613442291729 1.4350183903014029 9 0.2009165079948424 1.0963201198817578 >10 0.9422232729663342 9.688729052430048 >50 0.04091644062366467 1.6795420110912764 >100 0.013959783061680978 1.399960199955762 >500 1.0347245796015652E-4 0.034001909406114784 >1k 1.5520868542500042E-4 0.2378251610936867 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3362 0.1049591808063937 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.243853706507656E-5 7 0.0 0.0 0.0 3.121926853253828E-5 6.243853706507656E-5 8 0.0 0.0 0.0 3.121926853253828E-5 6.243853706507656E-5 9 0.0 0.0 0.0 3.121926853253828E-5 1.873156111952297E-4 10 0.0 0.0 0.0 3.121926853253828E-5 1.873156111952297E-4 11 0.0 0.0 0.0 3.121926853253828E-5 1.873156111952297E-4 12 0.0 0.0 0.0 3.121926853253828E-5 1.873156111952297E-4 13 0.0 0.0 0.0 3.121926853253828E-5 2.1853487972776797E-4 14 0.0 0.0 0.0 3.121926853253828E-5 2.1853487972776797E-4 15 0.0 0.0 0.0 3.121926853253828E-5 2.4975414826030625E-4 16 0.0 0.0 0.0 3.121926853253828E-5 3.1219268532538285E-4 17 0.0 0.0 0.0 6.243853706507656E-5 3.1219268532538285E-4 18 0.0 0.0 0.0 6.243853706507656E-5 3.746312223904594E-4 19 0.0 0.0 0.0 6.243853706507656E-5 3.746312223904594E-4 20 0.0 0.0 0.0 6.243853706507656E-5 3.746312223904594E-4 21 0.0 0.0 0.0 9.365780559761485E-5 3.746312223904594E-4 22 0.0 0.0 0.0 1.873156111952297E-4 3.746312223904594E-4 23 0.0 0.0 0.0 2.1853487972776797E-4 3.746312223904594E-4 24 0.0 0.0 0.0 2.4975414826030625E-4 4.058504909229977E-4 25 0.0 0.0 0.0 2.8097341679284455E-4 4.058504909229977E-4 26 0.0 0.0 0.0 3.434119538579211E-4 4.6828902798807425E-4 27 0.0 0.0 0.0 3.746312223904594E-4 4.6828902798807425E-4 28 0.0 0.0 0.0 5.931661021182274E-4 4.6828902798807425E-4 29 0.0 0.0 0.0 9.677973245086868E-4 4.6828902798807425E-4 30 0.0 0.0 0.0 0.0016234019636919908 4.6828902798807425E-4 31 0.0 0.0 0.0 0.0036838736868395174 4.6828902798807425E-4 32 0.0 0.0 0.0 0.005838003215584659 4.6828902798807425E-4 33 0.0 0.0 0.0 0.008585298846448028 4.6828902798807425E-4 34 0.0 0.0 0.0 0.0119881991164947 4.995082965206125E-4 35 0.0 0.0 0.0 0.016077923294257217 4.995082965206125E-4 36 0.0 0.0 0.0 0.020604717231475268 4.995082965206125E-4 37 0.0 0.0 0.0 0.02672369386385277 4.995082965206125E-4 38 0.0 0.0 0.0 0.0330299861074255 4.995082965206125E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2105 0.0 28.53873 1 GTATCAA 5025 0.0 24.61072 1 ATCAACG 7295 0.0 16.800154 3 TCAACGC 7335 0.0 16.739058 4 TATCAAC 7345 0.0 16.685791 2 CAACGCA 7520 0.0 16.357033 5 CGCGCAT 95 3.346896E-4 16.211527 10 AACGCAG 7775 0.0 15.732711 6 TCTATAC 600 0.0 14.668717 3 GTACATG 7320 0.0 14.459661 1 TACATGG 7405 0.0 13.876349 2 TATACTG 965 0.0 13.4535 5 ACGCAGA 9050 0.0 13.370155 7 ACATGGG 7585 0.0 13.227953 3 CGCAGAG 9290 0.0 13.001269 8 ACGCCCG 205 4.4233275E-7 12.878843 21 GGCGTGC 445 0.0 12.854124 8 TAGGACA 845 0.0 12.759579 4 CTATACT 795 0.0 12.731737 4 GTACCGA 195 3.5524936E-6 12.410441 6 >>END_MODULE