FastQCFastQC Report
Mon 6 Feb 2017
SRR2077413_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077413_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3130559
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20000.025.7404671
GTATCAA48600.025.3502521
TATCAAC63250.019.0606062
TCAACGC63450.018.6543984
ATCAACG64200.018.4692653
CAACGCA64350.018.289765
AACGCAG67100.017.6054696
ACGCAGA76900.015.3342347
GTACATG91050.015.2710081
TACATGG91850.014.8979982
CGCAGAG80500.014.621158
GAGTACT37800.014.60989812
CATTGCG2153.5470293E-914.3267589
ACATGGG93700.014.3214213
AGAGTAC78700.014.00623411
ACCGTCG1751.0541153E-613.8286018
AGTACTT41350.013.46202213
CAGAGTA84300.013.44119910
GCAGAGT86250.013.3413859
CGTTAAC1000.00875981513.2010849
Produced by FastQC (version 0.11.5)