FastQCFastQC Report
Mon 6 Feb 2017
SRR2077413_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077413_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3130559
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36020.11505932327101964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.028.6818451
GTATCAA54350.025.4233231
TATCAAC78550.017.505932
TCAACGC79050.017.2290364
ATCAACG79700.017.1434633
CAACGCA80650.016.8059255
GCGTATC1053.7737434E-516.7638171
AACGCAG84350.016.0925086
GCACCGT3750.015.2533676
TAATACT8900.013.8442764
GTGCCGA2104.019057E-813.61994711
GTATTAG5500.013.6015511
TCTATAC4900.013.4707093
ACGCAGA100000.013.4422457
TAGGACC4750.013.4331114
GTACATG86750.013.2141871
TACATGG85800.013.0008592
ATTACAC7450.012.9945623
CGCAGAG103550.012.8541338
TCGCTAA1404.3583446E-412.57306314
Produced by FastQC (version 0.11.5)