FastQCFastQC Report
Mon 6 Feb 2017
SRR2077412_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077412_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2852233
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38550.021.7427831
GGTATCA19850.019.0626161
TATGCGA951.5966765E-518.5285712
TCAACGC48250.016.7795494
ATCAACG48550.016.4928683
CAACGCA48950.016.4488415
GTACATG77450.016.4180261
TATCAAC49350.016.2712252
TACATGG79050.015.75176052
GTATTAG4300.015.348551
AACGCAG51900.015.343266
CGCGATC1301.3688921E-515.23235423
GTACCGT1901.0795702E-815.0510286
ACATGGG82750.014.647643
GTATAGA4750.013.4313261
GCACCGT3800.013.3143716
CGTGCCG2155.4631528E-813.30441110
TAAGGCG2653.4924597E-1013.2825375
GTATAAA8950.013.2734941
TCGCGTC3203.6379788E-1213.06317217
Produced by FastQC (version 0.11.5)