FastQCFastQC Report
Mon 6 Feb 2017
SRR2077412_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077412_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2852233
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19900.023.329471
GTATCAA41500.023.0630441
GTACCGT1753.6252459E-916.3448456
TATCAAC57600.016.272242
ATCAACG57950.016.2498953
TCAACGC58250.016.0912274
CAACGCA59450.015.8053765
TATACCG1301.3670759E-515.2344915
AACGCAG62350.015.0683936
TGTACCG3500.014.4606775
ATACCGT1851.2655983E-714.2720066
GTACATG70900.014.2132941
CCCGATT1251.6695575E-414.08269913
TAGCGCG1251.6705526E-414.0817124
TACATGG71550.013.4686712
TAGCGTG1150.001422073313.3945765
ACATGGG72750.013.0348043
TCGTATG3451.8189894E-1212.74801740
ACGCAGA73400.012.6498177
TACCGTG2105.9210834E-712.5727377
Produced by FastQC (version 0.11.5)