FastQCFastQC Report
Mon 6 Feb 2017
SRR2077411_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077411_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3164454
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34170.10798071326048664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53700.019.6247331
GGTATCA27200.017.3905011
TATCAAC66800.015.6768672
ATCAACG66500.015.647353
TTACGCG850.002988321215.52398243
TCAACGC67300.015.3976784
TACGCGA1004.959998E-415.39461444
CAACGCA67400.015.3743465
GTACATG66750.014.7991781
AACGCAG70050.014.7913286
TACATGG67250.014.19796852
GTGCCGA2754.3655746E-1113.6006211
TGGACCG4050.013.5806525
AGAGTAC68100.013.40736711
TGCGATA1851.9678737E-613.08209213
ACATGGG68900.013.0588643
GGGTACG1353.2085826E-413.0376331
ACGCAGA78300.013.0368077
AGCGTAC2054.4215813E-712.87924923
CGTACAA2054.422418E-712.87904525
Produced by FastQC (version 0.11.5)