FastQCFastQC Report
Mon 6 Feb 2017
SRR2077411_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077411_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3164454
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39420.12457125305028924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66500.020.3801331
GGTATCA34350.019.3431931
TATAGCG902.2168827E-417.1110252
TATCAAC90600.014.93372
ATCAACG92750.014.6824063
TCAACGC92650.014.6270194
CAACGCA92750.014.5410095
AACGCAG95800.014.0773966
GCGTAGT950.006239066413.89598511
GTACATG74650.013.380551
TACATGG74750.013.2146492
GTATAAT6700.013.1350931
AGAGTAC98600.012.45142311
TCTATAC4600.012.4347213
TATACTG9400.012.4049785
ACATGGG76100.012.3442213
GCACCGT3750.012.3201326
GAGTACT65900.012.28716212
ACGCAGA109250.012.2436097
TCGAACT4500.012.223919
Produced by FastQC (version 0.11.5)