FastQCFastQC Report
Mon 6 Feb 2017
SRR2077410_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077410_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3109793
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31320.10071409897700585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19300.027.9262961
GTATCAA46600.026.1062091
TCAACGC59150.019.7867954
ATCAACG60150.019.6391323
TATCAAC60150.019.5659872
CAACGCA60100.019.3272915
AACGCAG62650.018.5751446
ACGCAGA73150.015.7589957
GTACATG86700.015.5795131
CGTGCCG2550.015.53051510
GTATTCG850.002982178215.5287641
TACATGG86850.015.2479092
CGCAGAG76100.015.1194358
AGAGTAC74750.014.54016211
ACATGGG91650.013.9452813
TATACCG1901.7561433E-713.8943815
CAGAGTA79000.013.86933810
GCAGAGT82550.013.5125529
GAGTACT38850.013.365170512
GTATATG7550.013.1120361
Produced by FastQC (version 0.11.5)