FastQCFastQC Report
Mon 6 Feb 2017
SRR2077410_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077410_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3109793
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33100.10643795262257004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20500.025.9741881
GTATCAA48400.025.4124741
ATCAACG70250.017.225663
TCGATCG1154.2869124E-617.21331441
TATCAAC70950.017.055162
TCAACGC70950.016.9629524
CAACGCA71950.016.7279995
AACGCAG73950.016.2740156
TATTCCG1401.5856203E-615.716635
GTACATG80700.014.2869071
ATCGTCG1554.5846573E-614.18973244
CGTACTA2204.9076334E-914.00006129
CGCGATC1453.8371247E-513.65611123
TACATGG81900.013.6466272
ACGCAGA88300.013.5048917
GCCTTAT5250.013.4113091
ACCGTAC2351.2374585E-813.10728427
ACATGGG86200.012.9662973
GTGGTAT9000.012.9572791
CGCAGAG92400.012.8342148
Produced by FastQC (version 0.11.5)