##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077410_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3109793 Sequences flagged as poor quality 0 Sequence length 50 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.502460774720376 31.0 31.0 34.0 27.0 34.0 2 30.456704031425886 31.0 31.0 34.0 26.0 34.0 3 30.62093940014657 31.0 31.0 34.0 27.0 34.0 4 34.097688174100334 37.0 35.0 37.0 30.0 37.0 5 33.70564857532318 37.0 35.0 37.0 28.0 37.0 6 33.646935342641775 37.0 35.0 37.0 28.0 37.0 7 33.466856475656094 37.0 35.0 37.0 27.0 37.0 8 33.43473472350089 37.0 35.0 37.0 27.0 37.0 9 34.891768680423425 39.0 35.0 39.0 27.0 39.0 10 34.74620400779087 39.0 35.0 39.0 27.0 39.0 11 34.690054289787135 39.0 35.0 39.0 27.0 39.0 12 34.61694910240006 39.0 35.0 39.0 27.0 39.0 13 34.58615380509249 39.0 35.0 39.0 27.0 39.0 14 35.74154453367154 40.0 36.0 41.0 27.0 41.0 15 35.74347938914262 40.0 36.0 41.0 27.0 41.0 16 35.68142767058772 40.0 36.0 41.0 27.0 41.0 17 35.65434387433504 40.0 36.0 41.0 27.0 41.0 18 35.64684498292973 40.0 36.0 41.0 27.0 41.0 19 35.67765314282976 40.0 36.0 41.0 26.0 41.0 20 35.679391522200994 40.0 36.0 41.0 26.0 41.0 21 35.61826108683118 40.0 36.0 41.0 26.0 41.0 22 35.61533259609241 40.0 36.0 41.0 26.0 41.0 23 35.549347818327455 40.0 36.0 41.0 26.0 41.0 24 35.52861782118617 40.0 36.0 41.0 26.0 41.0 25 35.50806532782085 40.0 36.0 41.0 26.0 41.0 26 35.42805678705946 40.0 35.0 41.0 25.0 41.0 27 35.368748659476694 40.0 35.0 41.0 25.0 41.0 28 35.30225902495761 40.0 35.0 41.0 25.0 41.0 29 35.225790591206554 40.0 35.0 41.0 24.0 41.0 30 35.103841959898936 39.0 35.0 41.0 24.0 41.0 31 35.015260179696845 39.0 35.0 41.0 24.0 41.0 32 34.937107711027714 39.0 35.0 41.0 24.0 41.0 33 34.84268856480158 39.0 35.0 41.0 23.0 41.0 34 34.80985518971841 39.0 35.0 41.0 23.0 41.0 35 34.744099366099285 39.0 34.0 41.0 23.0 41.0 36 34.63719546606478 39.0 34.0 41.0 22.0 41.0 37 34.549056802173006 39.0 34.0 41.0 21.0 41.0 38 34.46913218982743 39.0 34.0 41.0 20.0 41.0 39 34.378312318536956 39.0 34.0 41.0 19.0 41.0 40 34.2866907861713 39.0 34.0 41.0 18.0 41.0 41 34.21942618045638 39.0 34.0 41.0 18.0 41.0 42 34.102950260676515 39.0 34.0 41.0 16.0 41.0 43 34.20601499842594 39.0 34.0 41.0 16.0 41.0 44 34.14021672825169 39.0 34.0 41.0 15.0 41.0 45 34.073027368702675 39.0 34.0 41.0 14.0 41.0 46 33.921027862626225 39.0 34.0 41.0 9.0 41.0 47 33.77529436846761 39.0 33.0 41.0 2.0 41.0 48 33.619404249736235 39.0 33.0 41.0 2.0 41.0 49 33.46919360870643 39.0 33.0 41.0 2.0 41.0 50 33.32402124514397 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6961943347075703 1101 2 0.8439207669275035 1101 3 0.734648783279674 1101 4 0.7181956697229097 1101 5 0.8188455695065855 1101 6 0.868476072147196 1101 7 0.8593342640092914 1101 8 0.8904179736022542 1101 9 0.9467849391380341 1101 10 0.9539110680646843 1101 11 0.9710931317774509 1101 12 0.9965313939046752 1101 13 0.906950187716248 1101 14 1.1210993823840099 1101 15 1.041746160674748 1101 16 1.048332648422715 1101 17 1.1496406654108853 1101 18 1.1241558535866432 1101 19 1.0837459351065917 1101 20 1.0350520202264306 1101 21 1.0870468926550387 1101 22 1.070726640170264 1101 23 1.1851873798986716 1101 24 1.0489007079958412 1101 25 1.0397539607816313 1101 26 1.060642716125102 1101 27 1.1129398454925905 1101 28 1.1253162404767636 1101 29 1.1809208805315095 1101 30 1.2879204074588486 1101 31 1.2799864677831678 1101 32 1.2583633579527316 1101 33 1.260730417969107 1101 34 1.2189356783637706 1101 35 1.2295153344805527 1101 36 1.2137844033599237 1101 37 1.2979148346866438 1101 38 1.2451939006010022 1101 39 1.2456473051461003 1101 40 1.3345738945539267 1101 41 1.3010840334532006 1101 42 1.40831633627365 1101 43 1.4123110130740173 1101 44 1.3642480015641354 1101 45 1.311179407475251 1101 46 1.2825828944762776 1101 47 1.3282339184024252 1101 48 1.4091305260108271 1101 49 1.4510288030208187 1101 50 1.4831373465703095 1102 1 0.588295498317617 1102 2 0.7754525995657175 1102 3 0.5729401238117724 1102 4 0.6512918056234227 1102 5 0.5950030853610073 1102 6 0.6641400535599047 1102 7 0.6511794714975281 1102 8 0.7035076344223015 1102 9 0.7730302494657906 1102 10 0.8121040445068175 1102 11 0.8417562561942162 1102 12 0.7997213232501181 1102 13 0.8700265174882844 1102 14 0.9001356428346057 1102 15 0.8796697547252919 1102 16 0.9516965479446995 1102 17 0.9701815250730377 1102 18 0.9073632085162728 1102 19 0.9954946829402971 1102 20 0.898232522616496 1102 21 0.9642327084199565 1102 22 0.8206846473690277 1102 23 0.8975797316859868 1102 24 0.8887872036442062 1102 25 0.8937930778853698 1102 26 0.8452942044413945 1102 27 0.8695947998813622 1102 28 0.9473791249346633 1102 29 0.9518105375426842 1102 30 0.9343792049983151 1102 31 0.9403779244858299 1102 32 1.018161106910057 1102 33 1.0455218822895134 1102 34 0.9821732185949728 1102 35 1.0866374468803315 1102 36 0.999450860538694 1102 37 0.9881934535900712 1102 38 0.9457964639710923 1102 39 0.9842077424044788 1102 40 0.9385933959727311 1102 41 0.9938867910757025 1102 42 0.9518554438552016 1102 43 0.9356977658211676 1102 44 1.0300063477524048 1102 45 0.9715253481711343 1102 46 0.9214657526529706 1102 47 1.0254150683005392 1102 48 1.0765195450361347 1102 49 1.0302558879015038 1102 50 0.9801157693168889 1103 1 0.44447464696319017 1103 2 0.6094532824703371 1103 3 0.5595161159204309 1103 4 0.4921431600509223 1103 5 0.5662524214259577 1103 6 0.6667961731440926 1103 7 0.6330919838277538 1103 8 0.6085156016428783 1103 9 0.7248179420069505 1103 10 0.7405883756396818 1103 11 0.7937044313213164 1103 12 0.7706827011998882 1103 13 0.7897055523164198 1103 14 0.8272268485406471 1103 15 0.877351293537032 1103 16 0.9347825180486495 1103 17 0.9170720326988047 1103 18 0.9525972930446898 1103 19 0.9940271967880818 1103 20 0.9348271049065033 1103 21 1.0099949058552724 1103 22 0.9555852468075301 1103 23 0.9989390912732077 1103 24 0.9780314558636434 1103 25 1.0084971525496087 1103 26 0.9490983625717533 1103 27 0.854605422842134 1103 28 0.8966487963816618 1103 29 0.9972620133754475 1103 30 1.1045510693279326 1103 31 1.06468781142717 1103 32 1.0532589899057285 1103 33 1.0638225879576169 1103 34 1.0107952688263993 1103 35 1.0551858951692736 1103 36 1.0050480226906018 1103 37 1.0475367269795512 1103 38 0.9914076636068714 1103 39 1.031865379554489 1103 40 1.0789200520164357 1103 41 1.0523677076854625 1103 42 1.0429344331563684 1103 43 1.1121648725819213 1103 44 1.1395969843712663 1103 45 1.2335991018702046 1103 46 1.2550286178172456 1103 47 1.2026603069377586 1103 48 1.2503032160278664 1103 49 1.1942148377459532 1103 50 1.259249998014056 1104 1 0.5326842626343691 1104 2 0.689743251693109 1104 3 0.5008652068512482 1104 4 0.5779212402106424 1104 5 0.5768655422130422 1104 6 0.6227075876828536 1104 7 0.5034110702284451 1104 8 0.5082018072374552 1104 9 0.48880947145333664 1104 10 0.6373024088384511 1104 11 0.6081092181681882 1104 12 0.5997594702369753 1104 13 0.6944161656986338 1104 14 0.7710512170397976 1104 15 0.8697568415050299 1104 16 0.8124387859632876 1104 17 0.880920553162241 1104 18 0.7135533945060999 1104 19 0.7095042569035783 1104 20 0.8018314117018122 1104 21 0.7800697556570881 1104 22 0.8378703766742888 1104 23 0.8237903402699871 1104 24 0.9032588678762821 1104 25 0.8445654148070005 1104 26 0.796059330652092 1104 27 0.7725335600112473 1104 28 0.856364135960078 1104 29 0.8496743527996173 1104 30 0.8286929314006812 1104 31 0.8453096320752138 1104 32 0.8601272584373305 1104 33 0.9460274229067451 1104 34 0.9707799231605136 1104 35 0.9732024967970574 1104 36 0.8844017811836551 1104 37 0.8689558350785447 1104 38 0.8393743722136335 1104 39 0.8375170400347116 1104 40 0.7934137794895122 1104 41 0.7960269300796057 1104 42 0.8256257152570612 1104 43 0.8671451648479547 1104 44 0.8309167116374425 1104 45 0.8105706035233453 1104 46 0.7440394647269599 1104 47 0.7770611107832508 1104 48 0.8715043327621075 1104 49 0.9431208661763293 1104 50 1.003157107624837 1105 1 0.3761030641832477 1105 2 0.5042915586804106 1105 3 0.45609889586344465 1105 4 0.40592508802375704 1105 5 0.4804309225606218 1105 6 0.4952943805497654 1105 7 0.5352281819665734 1105 8 0.5884534602314417 1105 9 0.5912876463264638 1105 10 0.566638702098146 1105 11 0.6187877883514616 1105 12 0.6659851561898833 1105 13 0.7170203129228341 1105 14 0.7418731526739322 1105 15 0.7609083526787117 1105 16 0.7744564220012791 1105 17 0.8145654672872311 1105 18 0.7749811647138998 1105 19 0.6457750459536769 1105 20 0.8394704260290098 1105 21 0.8409030793048018 1105 22 0.767469617503771 1105 23 0.7467147967413155 1105 24 0.8764058543827318 1105 25 0.7058493820016807 1105 26 0.6994524208603394 1105 27 0.7248740053265621 1105 28 0.8682662851884047 1105 29 0.8689802833407114 1105 30 0.9402705324975074 1105 31 0.9039499483780347 1105 32 0.88752444441063 1105 33 0.835849474084668 1105 34 0.8133988517847683 1105 35 0.8523259684342506 1105 36 0.9640348305345299 1105 37 0.968680558280532 1105 38 0.9749662560718448 1105 39 0.919165838436335 1105 40 1.0367236918471576 1105 41 0.8845780907932621 1105 42 1.0498442848979437 1105 43 0.9253956405259842 1105 44 0.9531222897813905 1105 45 0.9547005576909768 1105 46 1.0638651599780644 1105 47 1.1840711316426678 1105 48 1.0980276248620413 1105 49 1.1842515817478727 1105 50 1.28299950316881 1106 1 0.3842429962667566 1106 2 0.590939337328912 1106 3 0.4673198521865771 1106 4 0.42143536591874664 1106 5 0.4810987010751617 1106 6 0.5010391511809047 1106 7 0.45240087337520407 1106 8 0.5240185267748672 1106 9 0.4713909276172785 1106 10 0.451841078505538 1106 11 0.526921692344068 1106 12 0.43469534567287127 1106 13 0.4679369176341197 1106 14 0.4696110780729583 1106 15 0.6028973547617511 1106 16 0.6543795303528697 1106 17 0.5712188051751212 1106 18 0.5043493538084078 1106 19 0.4410342873519255 1106 20 0.47337106969516185 1106 21 0.490470061441556 1106 22 0.4600014826620722 1106 23 0.5428454321690737 1106 24 0.5159588379528373 1106 25 0.542579879453136 1106 26 0.5552718728398958 1106 27 0.5122431455198608 1106 28 0.46492538325204436 1106 29 0.49068074080583557 1106 30 0.58063797812747 1106 31 0.5216644981801082 1106 32 0.5796249661753876 1106 33 0.6687481150850658 1106 34 0.6303310893451339 1106 35 0.5111862573807429 1106 36 0.6522318285452542 1106 37 0.7305542228909516 1106 38 0.6080095712539304 1106 39 0.666458220938793 1106 40 0.6934708000379928 1106 41 0.7074479978936807 1106 42 0.726278468192092 1106 43 0.693123251191686 1106 44 0.6107742813956492 1106 45 0.5832020412711287 1106 46 0.6027436671548401 1106 47 0.646724429343287 1106 48 0.6720103845220535 1106 49 0.6406865687874799 1106 50 0.7402871301773359 1107 1 0.3734848154547876 1107 2 0.4770217711942948 1107 3 0.31632553808922736 1107 4 0.37121817974381344 1107 5 0.4358481086185293 1107 6 0.38624102881610867 1107 7 0.4312743364938143 1107 8 0.4437039242284442 1107 9 0.42073711295744687 1107 10 0.49839160287998396 1107 11 0.5100732740937914 1107 12 0.4982166946823625 1107 13 0.4431773840258941 1107 14 0.518049943228192 1107 15 0.5137469311613359 1107 16 0.6311807998048451 1107 17 0.6290506682604615 1107 18 0.562722025731432 1107 19 0.5072198221643092 1107 20 0.47077961106847255 1107 21 0.5698645147999457 1107 22 0.5115157252886036 1107 23 0.5282408507993566 1107 24 0.4868788942411939 1107 25 0.5225955219893308 1107 26 0.5266547464746623 1107 27 0.5675779095082305 1107 28 0.563821779319646 1107 29 0.5673202763428336 1107 30 0.6342575044010488 1107 31 0.5743556027705523 1107 32 0.4800659299785792 1107 33 0.6348106241814904 1107 34 0.5363607650412021 1107 35 0.49661547379850646 1107 36 0.5306629122465409 1107 37 0.5553079613288148 1107 38 0.5591067385658022 1107 39 0.5758633843028775 1107 40 0.4047284272121203 1107 41 0.4384078329392409 1107 42 0.40763005233066707 1107 43 0.44542284999779724 1107 44 0.4319434194149281 1107 45 0.48076904295172795 1107 46 0.45004176850661537 1107 47 0.5130256906112436 1107 48 0.42765131930515565 1107 49 0.5462705029826509 1107 50 0.5580379139630693 1108 1 0.4402963582090571 1108 2 0.566462345002023 1108 3 0.4989812194567804 1108 4 0.4432481482185011 1108 5 0.40347847992577357 1108 6 0.39917023933228535 1108 7 0.47809944785051073 1108 8 0.4159953182688838 1108 9 0.5699931308186876 1108 10 0.4883281357986249 1108 11 0.5324040410780455 1108 12 0.5839548554333476 1108 13 0.6807057421528384 1108 14 0.5451234610392177 1108 15 0.5890520660418161 1108 16 0.5738331094538083 1108 17 0.5251013581107813 1108 18 0.5615701378081184 1108 19 0.5152805162288416 1108 20 0.4693544122279363 1108 21 0.569267551937628 1108 22 0.5233573870189829 1108 23 0.5942944089579782 1108 24 0.6204222513860955 1108 25 0.5565443722011238 1108 26 0.6176865836535157 1108 27 0.5700309260704302 1108 28 0.4911207275573304 1108 29 0.5797103422756749 1108 30 0.6663627774889562 1108 31 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0.404193339962184 1109 19 0.39890288747589864 1109 20 0.25960728789166865 1109 21 0.29584653190688215 1109 22 0.39778417069706506 1109 23 0.3647677282192774 1109 24 0.3153255540703199 1109 25 0.3599793410660723 1109 26 0.4249627007580159 1109 27 0.37044667045770296 1109 28 0.368246162346189 1109 29 0.2974876283992529 1109 30 0.41560519084260505 1109 31 0.4312846845609357 1109 32 0.49210267848307154 1109 33 0.3343464525625137 1109 34 0.2948295814434445 1109 35 0.40398541741333815 1109 36 0.31675260833588936 1109 37 0.39685227202138407 1109 38 0.31278079157473115 1109 39 0.3705770909527928 1109 40 0.45448318363703777 1109 41 0.23308258769566947 1109 42 0.2252499260161187 1109 43 0.22110257587461035 1109 44 0.23382550129855417 1109 45 0.23747796986693004 1109 46 0.17547232494821685 1109 47 0.19585851767219253 1109 48 0.18492305507513862 1109 49 0.196589847167715 1109 50 0.007536323989718596 1110 1 0.1116632227765244 1110 2 0.15357798021639013 1110 3 0.13668181767318188 1110 4 0.13142120412717873 1110 5 0.11631720511484644 1110 6 0.1865743998406657 1110 7 0.14127573634858948 1110 8 0.06912749279752717 1110 9 0.09505558375661849 1110 10 0.17277585317701494 1110 11 0.17463220270768431 1110 12 0.16878476351409688 1110 13 0.10955987385003851 1110 14 0.17917267616824262 1110 15 0.06895854018829795 1110 16 0.1746307169672363 1110 17 0.24749265416247113 1110 18 0.1358860111960567 1110 19 0.26772227341016475 1110 20 0.22532626040158732 1110 21 0.3056943161065959 1110 22 0.27616060842676404 1110 23 0.263817857657493 1110 24 0.17748540106441624 1110 25 0.17148181244344585 1110 26 0.19082261575319137 1110 27 0.06778590242751648 1110 28 0.14517930491993525 1110 29 0.17022771250109514 1110 30 0.11455623596850728 1110 31 0.1870577870425265 1110 32 0.28855062049530744 1110 33 0.194108679733354 1110 34 0.2142420493270052 1110 35 0.1338597196034712 1110 36 0.1807400277886444 1110 37 0.22355292417883987 1110 38 0.22449238884996703 1110 39 0.21566998640269475 1110 40 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-1.7413380009838804 2216 47 -1.7073839728009688 2216 48 -1.7644225522469839 2216 49 -1.629386164305192 2216 50 -1.69747223215834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 79596.0 3 13149.0 4 22266.0 5 13222.0 6 9960.0 7 7598.0 8 6515.0 9 5695.0 10 5605.0 11 5441.0 12 5633.0 13 5759.0 14 6329.0 15 6814.0 16 7413.0 17 7862.0 18 8630.0 19 9520.0 20 11115.0 21 13881.0 22 17268.0 23 20710.0 24 24358.0 25 27790.0 26 30344.0 27 34497.0 28 40505.0 29 46508.0 30 53380.0 31 63766.0 32 77682.0 33 96249.0 34 121887.0 35 154843.0 36 202463.0 37 275191.0 38 437257.0 39 1133092.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.56867134162579 18.077516328266007 13.994613634053874 26.35919869605433 2 14.66959462805479 19.92674093973026 38.2405091540061 27.163155278208855 3 17.556312737637693 24.533889257618554 29.51260798205401 28.39719002268974 4 12.168017375164244 16.417298501540007 36.79655377997933 34.61813034331642 5 13.732658854941574 37.63978671899504 34.391545965595164 14.236008460468222 6 32.62374893694218 36.81132830442117 17.068269862937914 13.49665289569875 7 28.33893939184371 31.75227150714463 21.637788254715023 18.27100084629664 8 26.981528845980655 34.23895423188458 19.670968497271204 19.10854842486356 9 26.666233488848455 14.87106955463316 18.771406918664646 39.69129003785374 10 14.346978281610168 28.673787970512038 33.805199057898335 23.174034689979457 11 35.27981928505389 22.500966691136874 23.430791670178493 18.788422353630736 12 23.49516636947396 24.454043112941058 30.352291579339834 21.698498938245148 13 28.215310736971322 21.422730568524777 25.96062846735005 24.40133022715385 14 22.365351745933907 20.387163896101306 27.10012157180538 30.14736278615941 15 23.765743461991796 28.446898145526976 23.743997282370927 24.0433611101103 16 23.774205129788907 27.598577031763927 25.012031789661155 23.61518604878601 17 22.59912597221436 27.275856655325235 26.60376763626443 23.52124973619597 18 22.750011018909735 26.852101780623645 27.458447450095598 22.939439750371022 19 23.56582548160788 26.40615217515798 27.585246155855703 22.442776187378428 20 23.887740542766466 25.851814556667797 27.40689147083567 22.853553429730063 21 23.947243459159235 25.76974155780815 27.304045019875716 22.9789699631569 22 23.685983328882983 25.584856371667463 27.275774762978667 23.453385536470883 23 23.44853411675914 25.696668928281085 27.182803752027972 23.671993202931798 24 23.138187586598264 25.894051175774006 27.320704798480794 23.647056439146937 25 23.447458978238288 25.680023342200325 27.389061013667863 23.483456665893527 26 23.291883804057278 25.899720084582643 27.298621591094435 23.50977452026564 27 23.345603137775683 26.108947113089375 27.129811571319927 23.41563817781501 28 23.356155988445728 25.901080952921717 27.294407103708235 23.44835595492432 29 23.422546733368957 26.13488201370483 27.086776135026774 23.35579511789944 30 23.559099525868156 26.101442147004356 27.02419619710168 23.31526213002581 31 23.533657929004136 25.643133051628208 27.212766285970158 23.610442733397498 32 23.4152159724704 26.123979067157443 26.96417491127603 23.49663004909613 33 23.45010988912022 25.929972663613853 26.99110305003823 23.6288143972277 34 23.58300500136693 25.883054854220607 27.18820256340157 23.345737581010887 35 23.605474471571075 25.919150165223492 26.98189392375082 23.493481439454612 36 23.591953496166227 26.03656969576501 26.893640781492383 23.477836026576377 37 23.653697898424664 25.677472847857626 27.05501249662699 23.61381675709072 38 23.501963696619086 25.856727176589896 27.002460771899532 23.638848354891486 39 23.67935930264794 26.079176371229856 26.749506812008377 23.491957514113828 40 23.729830656951208 25.805857189441806 27.001240695587075 23.463071458019915 41 23.55805023030612 25.849662829785725 26.906983856542908 23.68530308336525 42 23.752144986969284 25.957365248509767 26.74055884146263 23.54993092305832 43 23.554992015322348 25.793531607168006 26.88048323539267 23.77099314211697 44 23.633577004758088 25.987409523638018 26.70945173286271 23.669561738741184 45 23.67026519286915 26.03116893307037 26.563567727937652 23.734998146122823 46 23.783051130873233 25.8491558043775 26.46167797245129 23.906115092297973 47 23.713608404956744 26.067083758872002 26.41890883739835 23.800398998772906 48 23.69816822583778 26.244078344007214 26.31797443671112 23.739778993443892 49 23.776646083289844 25.91389582129474 26.438400522736096 23.871057572679323 50 23.837685294100623 26.118710419302165 26.28743585571483 23.756168430882383 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 521.0 1 371.0 2 221.0 3 373.5 4 526.0 5 613.5 6 701.0 7 676.5 8 652.0 9 860.5 10 1069.0 11 1633.0 12 2197.0 13 4045.0 14 5893.0 15 8240.5 16 10588.0 17 12149.0 18 13710.0 19 14884.5 20 16059.0 21 17624.0 22 19189.0 23 22807.5 24 26426.0 25 31365.5 26 36305.0 27 42970.0 28 49635.0 29 56126.5 30 62618.0 31 70734.0 32 78850.0 33 88712.0 34 98574.0 35 109183.5 36 119793.0 37 128922.5 38 138052.0 39 147443.5 40 156835.0 41 167077.5 42 177320.0 43 184860.5 44 192401.0 45 201093.5 46 209786.0 47 214894.5 48 220003.0 49 222984.5 50 225966.0 51 225198.5 52 224431.0 53 219256.0 54 214081.0 55 204333.0 56 194585.0 57 181290.5 58 167996.0 59 153019.5 60 138043.0 61 120878.5 62 103714.0 63 89326.5 64 74939.0 65 62696.5 66 50454.0 67 40951.0 68 31448.0 69 25378.0 70 19308.0 71 15169.0 72 11030.0 73 9148.0 74 7266.0 75 5592.0 76 3918.0 77 3046.0 78 2174.0 79 1678.5 80 1183.0 81 902.0 82 621.0 83 495.5 84 370.0 85 272.5 86 175.0 87 132.0 88 89.0 89 68.0 90 47.0 91 34.0 92 21.0 93 13.0 94 5.0 95 4.0 96 3.0 97 2.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014824137812388156 2 2.25095368083985E-4 3 5.46660179632535E-4 4 0.002154484237375285 5 0.03916659404661339 6 0.02691497472661364 7 0.030677283021731675 8 0.023442074761889295 9 0.03167413393753218 10 0.03212432467370015 11 0.040227757924723606 12 0.0405171662551173 13 0.03701210980923811 14 0.043153997709815414 15 0.03861993386698086 16 0.04376497085175766 17 0.045822985645668377 18 0.04823472173228251 19 0.05013195412041895 20 0.04688414952377859 21 0.04749512266572084 22 0.04575867268335866 23 0.04482613472986787 24 0.04276811993595715 25 0.0405171662551173 26 0.04353987548367367 27 0.043379093077899396 28 0.04283243289826686 29 0.04032422736818817 30 0.03743014406425122 31 0.035532911676114776 32 0.03196354226792587 33 0.024117360866141253 34 0.020676617382571767 35 0.021576998854907707 36 0.023024040506876182 37 0.01781469055978967 38 0.01948682757984213 39 0.013859443377742505 40 0.007942650845249186 41 0.00758892955254578 42 0.006977956410603536 43 0.002797613860472385 44 0.004534063842834555 45 0.00302270922855637 46 9.0038147233594E-4 47 2.8940833039369505E-4 48 6.75286104251955E-4 49 6.431296230971E-5 50 2.25095368083985E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3109793.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.20379562126179 #Duplication Level Percentage of deduplicated Percentage of total 1 77.73346972185776 47.57583393788124 2 12.417189191192898 15.199582188966222 3 4.188157441391626 7.689933962177993 4 1.9063249909171707 4.66697300527193 5 1.0250808718554283 3.1369420088152236 6 0.6397701095613247 2.349381541811015 7 0.4357660573582465 1.866937569926601 8 0.2832669461481623 1.3869609822648874 9 0.22321329389701924 1.2295350737659625 >10 1.0872848833195452 11.530400363269706 >50 0.04880177805060086 1.9930134005316298 >100 0.011516366549747194 1.1414901797059018 >500 0.0 0.0 >1k 1.5834790049512338E-4 0.23301578561171454 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3310 0.10643795262257004 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.2156481154855E-5 0.0 5 3.2156481154855E-5 0.0 0.0 3.2156481154855E-5 0.0 6 3.2156481154855E-5 0.0 0.0 3.2156481154855E-5 9.646944346456501E-5 7 6.431296230971E-5 0.0 0.0 3.2156481154855E-5 9.646944346456501E-5 8 6.431296230971E-5 0.0 0.0 3.2156481154855E-5 1.2862592461942E-4 9 6.431296230971E-5 0.0 0.0 3.2156481154855E-5 1.6078240577427501E-4 10 6.431296230971E-5 0.0 0.0 3.2156481154855E-5 1.6078240577427501E-4 11 6.431296230971E-5 0.0 0.0 3.2156481154855E-5 1.6078240577427501E-4 12 9.646944346456501E-5 0.0 0.0 3.2156481154855E-5 2.25095368083985E-4 13 9.646944346456501E-5 0.0 0.0 3.2156481154855E-5 2.25095368083985E-4 14 9.646944346456501E-5 0.0 0.0 3.2156481154855E-5 2.25095368083985E-4 15 1.2862592461942E-4 0.0 0.0 3.2156481154855E-5 2.89408330393695E-4 16 1.2862592461942E-4 0.0 0.0 3.2156481154855E-5 3.53721292703405E-4 17 1.6078240577427501E-4 0.0 0.0 3.2156481154855E-5 3.53721292703405E-4 18 1.6078240577427501E-4 0.0 0.0 3.2156481154855E-5 4.5019073616797E-4 19 2.89408330393695E-4 0.0 0.0 3.2156481154855E-5 4.8234721732282504E-4 20 2.89408330393695E-4 0.0 0.0 3.2156481154855E-5 4.8234721732282504E-4 21 2.89408330393695E-4 0.0 0.0 3.2156481154855E-5 4.8234721732282504E-4 22 3.2156481154855003E-4 0.0 0.0 9.646944346456501E-5 4.8234721732282504E-4 23 3.2156481154855003E-4 0.0 0.0 1.9293888692913002E-4 5.1450369847768E-4 24 3.2156481154855003E-4 0.0 0.0 2.25095368083985E-4 5.1450369847768E-4 25 3.53721292703405E-4 0.0 0.0 2.5725184923884E-4 5.1450369847768E-4 26 3.53721292703405E-4 0.0 0.0 3.2156481154855003E-4 5.46660179632535E-4 27 3.53721292703405E-4 0.0 0.0 3.8587777385826004E-4 5.7881666078739E-4 28 3.53721292703405E-4 0.0 0.0 7.717555477165201E-4 5.7881666078739E-4 29 3.8587777385826004E-4 0.0 0.0 0.00135057220850391 5.7881666078739E-4 30 3.8587777385826004E-4 0.0 0.0 0.001897232388136445 6.109731419422451E-4 31 4.18034255013115E-4 0.0 0.0 0.003569369408188905 6.109731419422451E-4 32 4.18034255013115E-4 0.0 0.0 0.0053379758717059305 6.109731419422451E-4 33 4.18034255013115E-4 0.0 0.0 0.007621086033700635 6.109731419422451E-4 34 4.8234721732282504E-4 0.0 0.0 0.010643795262257005 6.109731419422451E-4 35 6.109731419422451E-4 0.0 0.0 0.014245321151600765 6.109731419422451E-4 36 6.109731419422451E-4 0.0 0.0 0.01845782018288677 6.109731419422451E-4 37 6.109731419422451E-4 3.2156481154855E-5 0.0 0.02418167382845096 6.109731419422451E-4 38 6.431296230971001E-4 3.2156481154855E-5 0.0 0.030548657097112252 6.109731419422451E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2050 0.0 25.974188 1 GTATCAA 4840 0.0 25.412474 1 ATCAACG 7025 0.0 17.22566 3 TCGATCG 115 4.2869124E-6 17.213314 41 TATCAAC 7095 0.0 17.05516 2 TCAACGC 7095 0.0 16.962952 4 CAACGCA 7195 0.0 16.727999 5 AACGCAG 7395 0.0 16.274015 6 TATTCCG 140 1.5856203E-6 15.71663 5 GTACATG 8070 0.0 14.286907 1 ATCGTCG 155 4.5846573E-6 14.189732 44 CGTACTA 220 4.9076334E-9 14.000061 29 CGCGATC 145 3.8371247E-5 13.656111 23 TACATGG 8190 0.0 13.646627 2 ACGCAGA 8830 0.0 13.504891 7 GCCTTAT 525 0.0 13.411309 1 ACCGTAC 235 1.2374585E-8 13.107284 27 ACATGGG 8620 0.0 12.966297 3 GTGGTAT 900 0.0 12.957279 1 CGCAGAG 9240 0.0 12.834214 8 >>END_MODULE