FastQCFastQC Report
Mon 6 Feb 2017
SRR2077409_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077409_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2662760
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32120.12062671814207813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40550.024.035441
GGTATCA18650.022.5316871
TCAACGC51550.018.5652074
TATCAAC51550.018.564162
ATCAACG52000.018.5297343
CAACGCA53150.018.0470455
AACGCAG54250.017.6395666
GTACATG61100.015.8794911
ACGCAGA62950.014.9576287
TACATGG62200.014.8550332
CGCAGAG64850.014.3500448
GAGTACT34350.014.34724912
AGAGTAC59900.014.25122711
ACATGGG64400.014.1762253
GTATTAC3300.014.0005321
GACCGCG1901.7556704E-713.8944817
TACCGTC950.006243195413.8944817
TATACTG6200.013.8387165
TACACCG2602.582965E-1013.5384675
CAGAGTA65200.013.39722210
Produced by FastQC (version 0.11.5)