FastQCFastQC Report
Mon 6 Feb 2017
SRR2077409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2662760
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39010.14650212561402454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51500.026.018091
GGTATCA21500.024.1512091
TATCAAC70950.018.7291152
ATCAACG73350.018.1166423
TCAACGC73850.018.024454
CAACGCA75450.017.7310355
AACGCAG77150.017.2525046
ACGCAGA90000.014.7156187
CGCAGAG91750.014.4354818
TACACCG2153.5415724E-914.3277955
GAGTACT63550.014.195209512
TAACACG1402.7550781E-514.1450425
AGAGTAC90700.013.68135111
GTATTAC4050.013.5815811
AGTACTT66050.013.55798213
CAGAGTA94850.013.36085210
GGCGAAT3300.013.33163732
GTACATG57600.013.0637831
GCAGAGT99100.013.0540279
CGCGTCC3051.8189894E-1112.98512618
Produced by FastQC (version 0.11.5)