##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077409_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2662760
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.43520294731782	31.0	31.0	34.0	27.0	34.0
2	30.394601841698087	31.0	31.0	34.0	26.0	34.0
3	30.56087217774039	31.0	31.0	34.0	27.0	34.0
4	34.021503252264566	37.0	35.0	37.0	30.0	37.0
5	33.65274038967087	37.0	35.0	37.0	28.0	37.0
6	33.59400959906262	37.0	35.0	37.0	28.0	37.0
7	33.40764244618366	37.0	35.0	37.0	27.0	37.0
8	33.36930703480599	37.0	35.0	37.0	27.0	37.0
9	34.81029796151362	39.0	35.0	39.0	27.0	39.0
10	34.666847932220705	39.0	35.0	39.0	27.0	39.0
11	34.61423335186048	39.0	35.0	39.0	27.0	39.0
12	34.53585602908261	39.0	35.0	39.0	27.0	39.0
13	34.50922201024501	39.0	35.0	39.0	26.0	39.0
14	35.646127326533374	40.0	36.0	41.0	27.0	41.0
15	35.64977466989139	40.0	36.0	41.0	27.0	41.0
16	35.59396641079181	40.0	36.0	41.0	26.0	41.0
17	35.57032289804564	40.0	36.0	41.0	26.0	41.0
18	35.567300845739005	40.0	36.0	41.0	26.0	41.0
19	35.6079977166549	40.0	36.0	41.0	26.0	41.0
20	35.61147643798164	40.0	36.0	41.0	26.0	41.0
21	35.548577415914316	40.0	36.0	41.0	26.0	41.0
22	35.54848202616834	40.0	36.0	41.0	26.0	41.0
23	35.48491114482717	40.0	35.0	41.0	25.0	41.0
24	35.46791562138533	40.0	35.0	41.0	25.0	41.0
25	35.44374821613664	40.0	35.0	41.0	25.0	41.0
26	35.363700070603436	40.0	35.0	41.0	25.0	41.0
27	35.30068387687963	40.0	35.0	41.0	25.0	41.0
28	35.239573975874656	40.0	35.0	41.0	25.0	41.0
29	35.15529413090177	39.0	35.0	41.0	24.0	41.0
30	35.01841885862789	39.0	35.0	41.0	24.0	41.0
31	34.93007668734696	39.0	35.0	41.0	24.0	41.0
32	34.8413653502381	39.0	34.0	41.0	24.0	41.0
33	34.73905308777359	39.0	34.0	41.0	23.0	41.0
34	34.70841532845619	39.0	34.0	41.0	23.0	41.0
35	34.63696352656642	39.0	34.0	41.0	22.0	41.0
36	34.52253639081254	39.0	34.0	41.0	21.0	41.0
37	34.427970226381646	39.0	34.0	41.0	19.0	41.0
38	34.345472742567864	39.0	34.0	41.0	19.0	41.0
39	34.248385509771815	39.0	34.0	41.0	18.0	41.0
40	34.153322492451444	39.0	34.0	41.0	16.0	41.0
41	34.085611921464945	39.0	33.0	41.0	16.0	41.0
42	33.96381311120792	39.0	33.0	41.0	15.0	41.0
43	34.05100121678259	39.0	34.0	41.0	15.0	41.0
44	33.97861842599408	39.0	34.0	41.0	13.0	41.0
45	33.8977132749478	39.0	33.0	41.0	9.0	41.0
46	33.73392269675074	39.0	33.0	41.0	5.0	41.0
47	33.57155357598882	39.0	33.0	41.0	2.0	41.0
48	33.40476723399781	39.0	33.0	41.0	2.0	41.0
49	33.25152623593564	39.0	33.0	41.0	2.0	41.0
50	33.10162350343253	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5794687532051377
1101	2	0.7663417288138064
1101	3	0.6717864084795906
1101	4	0.6749022291894207
1101	5	0.6969625401390829
1101	6	0.7690058972535638
1101	7	0.6956715152831379
1101	8	0.7600504536490291
1101	9	0.8124315715427315
1101	10	0.8101330449755935
1101	11	0.9394637541564137
1101	12	0.9801059150919258
1101	13	0.9885260088766827
1101	14	1.0616539361185318
1101	15	1.1128409861545947
1101	16	1.0623048520278786
1101	17	1.0497253445684507
1101	18	1.1212633234466693
1101	19	1.0237591009534697
1101	20	0.9751796066233069
1101	21	1.0750016010685357
1101	22	1.1401706090531007
1101	23	1.1471715335672812
1101	24	1.037471140034782
1101	25	1.086511315798525
1101	26	1.1543626848956094
1101	27	1.1243952694860084
1101	28	1.2445515903296638
1101	29	1.1745190899914846
1101	30	1.2101729536554657
1101	31	1.2318438629279598
1101	32	1.0744549294349781
1101	33	1.172748089484685
1101	34	1.182514473613793
1101	35	1.0953844183763124
1101	36	1.135981865443327
1101	37	1.194160422422435
1101	38	1.2511038090024513
1101	39	1.1833621826578309
1101	40	1.2836649135172138
1101	41	1.3761326564194718
1101	42	1.3812724359661601
1101	43	1.3270549998934484
1101	44	1.3522805246978535
1101	45	1.3685613594713786
1101	46	1.384106616157439
1101	47	1.464510990004534
1101	48	1.5001141612898365
1101	49	1.5094201889661107
1101	50	1.580782770583447
1102	1	0.5329689382622895
1102	2	0.7217782303327951
1102	3	0.5895598905677595
1102	4	0.6234245035219033
1102	5	0.6656795604403811
1102	6	0.6552080505735063
1102	7	0.67320253813363
1102	8	0.6838875874375674
1102	9	0.6905794877413314
1102	10	0.8245244493513368
1102	11	0.7554160061554143
1102	12	0.7813633992336335
1102	13	0.838740787327275
1102	14	0.8957645818431672
1102	15	0.8379240267068937
1102	16	0.8607057990573992
1102	17	0.9456040150947231
1102	18	0.8829417321071205
1102	19	1.0003069511610079
1102	20	0.8967198971648429
1102	21	1.025756319483186
1102	22	0.8874478449251697
1102	23	0.910164698210977
1102	24	0.8926410362472055
1102	25	0.8398684840587123
1102	26	0.8038070973816716
1102	27	0.8556049137312769
1102	28	0.8860772571956801
1102	29	0.8895560417257613
1102	30	0.8783265898023558
1102	31	0.9175218504612985
1102	32	1.054438185652117
1102	33	0.970628235403062
1102	34	1.0337221931199707
1102	35	0.9048904225130485
1102	36	0.890161751123955
1102	37	0.8018631414058248
1102	38	0.8327033395397763
1102	39	0.8859673821306302
1102	40	0.8548476982456421
1102	41	0.8944956441210365
1102	42	0.7250275802112398
1102	43	0.8427910081799581
1102	44	0.7520748815220486
1102	45	0.7712397496803902
1102	46	0.8617114447923413
1102	47	0.8274247048920671
1102	48	0.8230557125354707
1102	49	0.8851079123507191
1102	50	0.9427098757939518
1103	1	0.5538693928994292
1103	2	0.6626373035011213
1103	3	0.6454861753905021
1103	4	0.6076865253898944
1103	5	0.5784555874834894
1103	6	0.5786400491449868
1103	7	0.5977007628999473
1103	8	0.5415745489575841
1103	9	0.6683658189078514
1103	10	0.7499079617212985
1103	11	0.8373911576907389
1103	12	0.8603621139109237
1103	13	0.7586061770861505
1103	14	0.929159887682502
1103	15	0.9414589401106568
1103	16	0.8998543467922815
1103	17	0.9679975543348718
1103	18	0.8309415001923952
1103	19	0.8741313324848505
1103	20	0.8264894939150906
1103	21	0.978249227776395
1103	22	0.9499718308605054
1103	23	0.9202391026046328
1103	24	0.9936074405974225
1103	25	0.9587873911089133
1103	26	0.9062778022724913
1103	27	0.9132280096849286
1103	28	0.8688366994870407
1103	29	0.8963758065340741
1103	30	0.9827287503598683
1103	31	0.9134136127468011
1103	32	0.9463105535044889
1103	33	0.8905936191029582
1103	34	0.9061848765383829
1103	35	0.8649015102685027
1103	36	0.9470394916478071
1103	37	0.9107350014551372
1103	38	0.8506153105710865
1103	39	0.8589970875710264
1103	40	0.8677306564948779
1103	41	0.810595920774901
1103	42	0.8380048888265463
1103	43	0.8535955167020219
1103	44	0.8180376501875202
1103	45	0.7425860556401318
1103	46	0.8461545239387078
1103	47	0.918910318731136
1103	48	0.8931735410041455
1103	49	0.9943665738882004
1103	50	0.9597499115282915
1104	1	0.47153329883241213
1104	2	0.5860311118719999
1104	3	0.501938039055112
1104	4	0.46093886950382057
1104	5	0.4871926636107773
1104	6	0.5221862531258594
1104	7	0.5454235626389163
1104	8	0.49204034949347175
1104	9	0.5781961481663274
1104	10	0.567607810626491
1104	11	0.6775624043616304
1104	12	0.5943950774045632
1104	13	0.7139987746719711
1104	14	0.7328066294765208
1104	15	0.735548362525769
1104	16	0.7898335038744975
1104	17	0.781449993758649
1104	18	0.731788030534986
1104	19	0.6132485092253575
1104	20	0.7357735795440874
1104	21	0.8092738393157006
1104	22	0.8496138209476456
1104	23	0.7960688294162495
1104	24	0.8511886123006107
1104	25	0.6965443198812054
1104	26	0.785126950837622
1104	27	0.759833407752609
1104	28	0.8048674583291415
1104	29	0.7184510834071887
1104	30	0.7985065545700536
1104	31	0.7082931207222103
1104	32	0.650004431787714
1104	33	0.7157008073380311
1104	34	0.7747524262616068
1104	35	0.8339595068846464
1104	36	0.8855037210852146
1104	37	0.8721412865913649
1104	38	0.8922401709245946
1104	39	0.838697232102902
1104	40	0.8630805160545307
1104	41	0.8620577027792677
1104	42	0.8777725090571451
1104	43	0.8698681804073587
1104	44	0.845901495310251
1104	45	0.8009099937833426
1104	46	0.7286984757250465
1104	47	0.8604822219665991
1104	48	0.8469375407800399
1104	49	0.8548535826040009
1104	50	0.8210360784444788
1105	1	0.5900017110143843
1105	2	0.6983092343390034
1105	3	0.562094166108718
1105	4	0.5934399992491208
1105	5	0.6219111091230687
1105	6	0.6289258787701826
1105	7	0.6376536511067314
1105	8	0.7434382622574418
1105	9	0.7847698803202334
1105	10	0.7880054774867844
1105	11	0.7375970609216722
1105	12	0.8288812302039901
1105	13	0.8182399770799762
1105	14	0.7870689105878199
1105	15	0.8902310151973651
1105	16	0.9198363846596607
1105	17	0.9606510385791722
1105	18	0.8225761579357496
1105	19	0.8515279572369465
1105	20	0.8207999623427469
1105	21	0.9966627273622564
1105	22	0.9242815764918504
1105	23	0.9731240117012874
1105	24	0.906320531522212
1105	25	0.8455215191550352
1105	26	0.8881849922975746
1105	27	1.0234078225462895
1105	28	0.9450686322883044
1105	29	0.9179746472015964
1105	30	1.025313402020366
1105	31	1.0255625476794918
1105	32	0.9991013936485942
1105	33	0.9950592409716563
1105	34	0.8953794231397936
1105	35	0.95728307900945
1105	36	1.0376055178900003
1105	37	1.0394342146907718
1105	38	0.9700133536205797
1105	39	1.044919100660671
1105	40	1.0728016759182424
1105	41	1.1454563809641982
1105	42	1.183001717953104
1105	43	1.0879322892923184
1105	44	1.1318608133257513
1105	45	1.1091735251519168
1105	46	1.09644002555261
1105	47	1.1481567660906222
1105	48	1.0596907575260985
1105	49	1.2321658571015206
1105	50	1.2073443147887701
1106	1	0.45170894408436624
1106	2	0.5921782517953993
1106	3	0.5025078389351023
1106	4	0.4976954007138872
1106	5	0.48154389681187126
1106	6	0.48060942477242463
1106	7	0.5194585246233387
1106	8	0.5648972542614317
1106	9	0.468558903269809
1106	10	0.4961810551323822
1106	11	0.6199767013347071
1106	12	0.5664022459067368
1106	13	0.5852312655327765
1106	14	0.5853781742677526
1106	15	0.5787347544778711
1106	16	0.6548938352704283
1106	17	0.666940798471849
1106	18	0.6131288196916742
1106	19	0.6651099832879623
1106	20	0.5104427465507371
1106	21	0.6149083207635044
1106	22	0.6193767365797314
1106	23	0.5990012003850254
1106	24	0.6171340301531814
1106	25	0.6316948790074264
1106	26	0.5740427290079069
1106	27	0.5731835929577471
1106	28	0.6498185350370349
1106	29	0.5932044694187653
1106	30	0.6256949674554448
1106	31	0.7024982720843838
1106	32	0.6270413352078492
1106	33	0.7220505248391618
1106	34	0.6131738061861682
1106	35	0.5270230004277892
1106	36	0.6570490772767243
1106	37	0.7372133206760552
1106	38	0.6348508309441385
1106	39	0.5949278041262929
1106	40	0.7313171185945038
1106	41	0.7149516768227073
1106	42	0.6812076921013244
1106	43	0.6299089864724152
1106	44	0.5849218511284846
1106	45	0.5264678185844076
1106	46	0.610422628823656
1106	47	0.6952144301469403
1106	48	0.6818896788007436
1106	49	0.7443499351987484
1106	50	0.7366096534504436
1107	1	0.3785393644826165
1107	2	0.49920954834298215
1107	3	0.39730464465450765
1107	4	0.4217607485099535
1107	5	0.34927180631743227
1107	6	0.4004015759097399
1107	7	0.44235812718994794
1107	8	0.4473530337586382
1107	9	0.39145946409129095
1107	10	0.5431949554083815
1107	11	0.5259985045640576
1107	12	0.5913176615951627
1107	13	0.4921469514490724
1107	14	0.5422667951208524
1107	15	0.46025533359299686
1107	16	0.6738891523916308
1107	17	0.6805835857466533
1107	18	0.6532242889330178
1107	19	0.6251368888299069
1107	20	0.6031287084495034
1107	21	0.673728315574273
1107	22	0.5895009427571765
1107	23	0.7439170692716317
1107	24	0.5737519158224416
1107	25	0.5688563014928079
1107	26	0.5793787430498369
1107	27	0.6622098667842593
1107	28	0.6858594024651197
1107	29	0.597089403752662
1107	30	0.7709704073888446
1107	31	0.7127331668451689
1107	32	0.7370375512284824
1107	33	0.657426380300052
1107	34	0.6018748539476775
1107	35	0.5580007309792592
1107	36	0.6473230722181569
1107	37	0.6137897556218519
1107	38	0.6066864803137193
1107	39	0.48403702900350964
1107	40	0.5516990079608206
1107	41	0.632727152408826
1107	42	0.6323963311669019
1107	43	0.6186695587715363
1107	44	0.5932466057320909
1107	45	0.6505943481353214
1107	46	0.7604898504025641
1107	47	0.8067842697626944
1107	48	0.7941034656870016
1107	49	0.7548935571304511
1107	50	0.8081380806729328
1108	1	0.38124792715251843
1108	2	0.5116838090162119
1108	3	0.4341720445799204
1108	4	0.3950146687425615
1108	5	0.45844033226725855
1108	6	0.4588664314533446
1108	7	0.41558130215329214
1108	8	0.4315224800992965
1108	9	0.4462265124695648
1108	10	0.4810548879430314
1108	11	0.479095163025427
1108	12	0.4544365752473283
1108	13	0.5177922718870036
1108	14	0.5883893079929763
1108	15	0.6212315492887655
1108	16	0.5159141133909486
1108	17	0.5272741835116861
1108	18	0.5761035187612151
1108	19	0.5509882362961349
1108	20	0.5684930103815375
1108	21	0.4797584914658799
1108	22	0.5234454905445673
1108	23	0.6800304794081882
1108	24	0.5314052817408879
1108	25	0.5277056415234043
1108	26	0.47735908237756064
1108	27	0.47713278861905195
1108	28	0.4291405634559524
1108	29	0.49774947152153004
1108	30	0.6594513016208978
1108	31	0.5986097063217883
1108	32	0.4762855174457812
1108	33	0.5503808517273256
1108	34	0.5765277682422862
1108	35	0.5721497376175932
1108	36	0.5352153659754322
1108	37	0.48267635813189
1108	38	0.5377986974551376
1108	39	0.6109729481620008
1108	40	0.6466336545911631
1108	41	0.6221755108952891
1108	42	0.6405152181207043
1108	43	0.5227893405419479
1108	44	0.45461747939517494
1108	45	0.48309934845710956
1108	46	0.5672610603525641
1108	47	0.45537854490362406
1108	48	0.3065302594865713
1108	49	0.37094309354856136
1108	50	0.4185900108378888
1109	1	0.24311611456369064
1109	2	0.3175411144666178
1109	3	0.28615841359821914
1109	4	0.27024296363071443
1109	5	0.16877967747553413
1109	6	0.14302417736806206
1109	7	0.17548816885962282
1109	8	0.15831108492081825
1109	9	0.16838155691458212
1109	10	0.10974019972950089
1109	11	0.12629681467569753
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2211	15	-0.4571573348610798
2211	16	-0.3357405559272806
2211	17	-0.30866237412096353
2211	18	-0.41184513496759223
2211	19	-0.2709036701062928
2211	20	-0.2809234559962235
2211	21	-0.2703742728916225
2211	22	-0.3221094584740811
2211	23	-0.21875072662034967
2211	24	-0.2666461631871755
2211	25	-0.23603196061848308
2211	26	-0.3128528473100616
2211	27	-0.11926650791926363
2211	28	-0.28347751478985117
2211	29	-0.324703797959188
2211	30	-0.3309725495783695
2211	31	-0.33212509936095813
2211	32	-0.30232294638761914
2211	33	-0.36596356760473014
2211	34	-0.36043800954045935
2211	35	-0.3997695719586929
2211	36	-0.5154398165220755
2211	37	-0.3826153078420873
2211	38	-0.4564418122030176
2211	39	-0.4481642729245152
2211	40	-0.3101676060365932
2211	41	-0.31742364239978116
2211	42	-0.3519693081477797
2211	43	-0.45864779581411597
2211	44	-0.42699808419040863
2211	45	-0.5255214638378263
2211	46	-0.5895553530993283
2211	47	-0.552220105840945
2211	48	-0.49584889742228455
2211	49	-0.44664786359484054
2211	50	-0.49542376242516895
2212	1	-0.8383624224630424
2212	2	-2.1873848593066967
2212	3	-1.1808653653587236
2212	4	-0.6621626310500304
2212	5	-0.5950306793862197
2212	6	-0.5251430402348589
2212	7	-0.49974596433595764
2212	8	-0.5262663487368826
2212	9	-0.49569395220751744
2212	10	-0.4553403119576984
2212	11	-0.5122135034616164
2212	12	-0.4844130702018177
2212	13	-1.9256559529789001
2212	14	-1.19774772380908
2212	15	-0.9589747982042169
2212	16	-0.792283492225657
2212	17	-0.6987408881223445
2212	18	-0.6979418967258155
2212	19	-0.5974491419682266
2212	20	-0.605902153821873
2212	21	-0.6117857489844454
2212	22	-0.5682486219762097
2212	23	-0.6790761470621689
2212	24	-0.5494523718064457
2212	25	-0.6609168868269251
2212	26	-0.7613202075404715
2212	27	-0.8614835210319427
2212	28	-0.6569045902507469
2212	29	-0.7679573339453754
2212	30	-0.589065942308217
2212	31	-0.6006429731726826
2212	32	-0.5789731365330368
2212	33	-0.6097635467544578
2212	34	-0.6432074653215807
2212	35	-0.6515364523652281
2212	36	-0.5796525950306304
2212	37	-0.5388035109892826
2212	38	-0.6259786680091821
2212	39	-0.5962031758856128
2212	40	-0.5179954035351173
2212	41	-0.5912292284052185
2212	42	-0.5383230721817753
2212	43	-0.6785206943422608
2212	44	-0.5563639786815813
2212	45	-0.6170763333404068
2212	46	-0.5342437515608225
2212	47	-0.5270482890531341
2212	48	-0.39581553589356844
2212	49	-0.39973518664535135
2212	50	-0.3177365142237534
2213	1	-0.8569913506285864
2213	2	-1.9176534821496674
2213	3	-1.0534454713954098
2213	4	-0.7243516218159556
2213	5	-0.743107157446083
2213	6	-0.6580421819650795
2213	7	-0.6938011562081954
2213	8	-0.6533812972355548
2213	9	-0.6257880756221539
2213	10	-0.5627932202529351
2213	11	-0.7093726596248899
2213	12	-0.5747096678057915
2213	13	-0.78068875304335
2213	14	-0.8053936442421445
2213	15	-0.8422699919277221
2213	16	-1.0142623637388795
2213	17	-0.9555041122821137
2213	18	-0.9904103094152461
2213	19	-0.9915989543556307
2213	20	-0.9860267032173766
2213	21	-1.0909666644379357
2213	22	-0.966554709057938
2213	23	-0.9658618435942898
2213	24	-1.0567417511678627
2213	25	-0.9509126529016996
2213	26	-1.0872930247120323
2213	27	-1.1047629789523867
2213	28	-0.9688691144141188
2213	29	-1.007016751172138
2213	30	-0.9921656212498391
2213	31	-1.0770864074174256
2213	32	-0.9167366640955521
2213	33	-0.9239120563956362
2213	34	-0.9477530211571121
2213	35	-1.009576367823854
2213	36	-1.049542701530136
2213	37	-0.9010252418247688
2213	38	-0.9530908738842285
2213	39	-0.8596846280362556
2213	40	-1.017520586819515
2213	41	-0.9304950280599869
2213	42	-0.9267944537612891
2213	43	-0.9956890040118935
2213	44	-0.9512015039437216
2213	45	-0.9993439002732813
2213	46	-0.9558369416263517
2213	47	-0.9765336185879363
2213	48	-1.0342396929224833
2213	49	-1.0325277088820286
2213	50	-1.0044521024393802
2214	1	-0.9815023556292175
2214	2	-1.7656580114137626
2214	3	-1.0214529903861873
2214	4	-0.6539916690330188
2214	5	-0.6995940911377261
2214	6	-0.6203662992654557
2214	7	-0.7837652966703672
2214	8	-0.7407104690068067
2214	9	-0.8895844328521036
2214	10	-0.8587740997480466
2214	11	-0.8292223829033532
2214	12	-0.7117425301712643
2214	13	-0.7294832116250376
2214	14	-0.9209105548120888
2214	15	-0.9170497000456308
2214	16	-0.8714484467330053
2214	17	-0.9645019868269955
2214	18	-0.993069963397339
2214	19	-0.9865640140728402
2214	20	-0.8976180797459605
2214	21	-0.9349716610616028
2214	22	-0.8313766799980229
2214	23	-0.8612003900487437
2214	24	-0.7721077435423354
2214	25	-0.9472071100515222
2214	26	-0.9527599060433687
2214	27	-0.9577581753436348
2214	28	-1.039463663374903
2214	29	-0.9295578584821342
2214	30	-0.8186500749574748
2214	31	-0.8940086040761486
2214	32	-0.8173188016813953
2214	33	-0.8123570491475576
2214	34	-0.9271776877768119
2214	35	-0.9655442231073366
2214	36	-1.0370862515957455
2214	37	-1.0240324486697503
2214	38	-1.0437849200325502
2214	39	-1.0196494386095765
2214	40	-1.0759322286548354
2214	41	-1.0112098018344753
2214	42	-0.9391515325119499
2214	43	-0.9065803721211338
2214	44	-0.8277925167794962
2214	45	-1.0072837437650932
2214	46	-1.0242209829006157
2214	47	-0.9789544380823259
2214	48	-0.9715153417132214
2214	49	-1.0016285972661407
2214	50	-1.127580387487484
2215	1	-0.48327669382920746
2215	2	-0.3378488383326754
2215	3	-0.6393879409108258
2215	4	-0.577611498184261
2215	5	-0.7723353060388405
2215	6	-0.8192055002035232
2215	7	-0.8201430563538921
2215	8	-0.8328035669238858
2215	9	-0.9627499393635048
2215	10	-1.0633882417887222
2215	11	-0.9380881072155489
2215	12	-0.9828079852366756
2215	13	-1.007111911882859
2215	14	-1.1212392013084909
2215	15	-1.1592730103472917
2215	16	-1.1653530688955698
2215	17	-1.2355919320286475
2215	18	-1.1549469252868434
2215	19	-1.0604868223879294
2215	20	-1.126783750341275
2215	21	-1.1233013653092598
2215	22	-1.3154540561381864
2215	23	-1.2155456109127627
2215	24	-1.1957293259646988
2215	25	-1.2369240302365583
2215	26	-1.2754555219805894
2215	27	-1.3044202282170616
2215	28	-1.5411079255558349
2215	29	-1.4080782557321783
2215	30	-1.2726067542441655
2215	31	-1.2636991773273394
2215	32	-1.231324332110752
2215	33	-1.2131113564755012
2215	34	-1.1934653955596133
2215	35	-1.2490533390317893
2215	36	-1.2543246135907964
2215	37	-1.3273042139861246
2215	38	-1.2010113163696658
2215	39	-1.0967127729405135
2215	40	-1.1914127944237407
2215	41	-1.4063512482341665
2215	42	-1.345611262351234
2215	43	-1.1530657453594344
2215	44	-1.2264105606554665
2215	45	-1.1369306288396217
2215	46	-1.1128441068100727
2215	47	-1.0422587686007603
2215	48	-1.019847738762671
2215	49	-1.0163745535689443
2215	50	-0.9978872494511108
2216	1	-1.5123198046283903
2216	2	-1.1707744535806128
2216	3	-1.2285608704056799
2216	4	-2.6897028090742445
2216	5	-1.9851394275837322
2216	6	-1.866147143082891
2216	7	-1.8109093193886352
2216	8	-1.704987180159275
2216	9	-1.8355994173002514
2216	10	-1.943393710958638
2216	11	-1.9496708257162965
2216	12	-1.9824930364865736
2216	13	-2.0097145497470237
2216	14	-2.2863489955680194
2216	15	-2.204342876972291
2216	16	-2.1834892943331354
2216	17	-2.187863658171416
2216	18	-2.2006527512998915
2216	19	-2.173896122401537
2216	20	-2.366756108132755
2216	21	-2.2458674836669417
2216	22	-2.3488428669709123
2216	23	-2.329435955403113
2216	24	-2.4594863762770203
2216	25	-2.443223544073156
2216	26	-2.646558906804053
2216	27	-2.5544649514784084
2216	28	-2.678842638485037
2216	29	-2.5722916988440545
2216	30	-2.7396462494640303
2216	31	-2.475389730077218
2216	32	-2.441446607123787
2216	33	-2.321147331759164
2216	34	-2.30672503429804
2216	35	-2.3339972935110467
2216	36	-2.47934822127619
2216	37	-2.5809617550419404
2216	38	-2.6636223331099096
2216	39	-2.8076344762501684
2216	40	-2.734991234578761
2216	41	-2.7199296075640156
2216	42	-2.8915045354069555
2216	43	-2.704962510062547
2216	44	-2.583223516391566
2216	45	-2.621048617424414
2216	46	-2.6021642728249788
2216	47	-2.6598974477558173
2216	48	-2.5797866079915863
2216	49	-2.6135537550970795
2216	50	-2.64477729892959
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	70280.0
3	10768.0
4	18815.0
5	11039.0
6	8321.0
7	6464.0
8	5661.0
9	4939.0
10	4613.0
11	4686.0
12	4723.0
13	5091.0
14	5361.0
15	5897.0
16	6468.0
17	7121.0
18	7435.0
19	8630.0
20	9890.0
21	12362.0
22	15576.0
23	18820.0
24	22090.0
25	24791.0
26	26691.0
27	30320.0
28	35852.0
29	41416.0
30	47101.0
31	55736.0
32	68577.0
33	84377.0
34	106771.0
35	136049.0
36	175370.0
37	236021.0
38	371217.0
39	947421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.150126863275005	18.483763191057406	14.367270589693556	25.99883935597403
2	14.660462531077012	20.328158212968596	38.239783080586164	26.771596175368227
3	17.567521384854523	24.695906373450896	29.81554025557798	27.921031986116603
4	12.121340720775057	16.93799979494629	37.00430800569494	33.93635147858372
5	13.438499812904903	37.8181931572786	34.655910326597414	14.087396703219088
6	32.17354458557812	37.386170435754714	17.087713656993326	13.352571321673839
7	27.740709158048553	32.22574501414334	21.78149757891534	18.252048248892763
8	26.448901092709388	34.5735653728697	19.821237402289093	19.156296132131818
9	26.254478300738203	15.203739439812802	19.046109264339673	39.495672995109324
10	14.105348334259341	29.049098618353558	33.99761605037474	22.84793699701236
11	34.98575582061538	22.69586357145514	23.6758060086656	18.642574599263877
12	23.433501290106378	24.715417710448133	30.537990138988864	21.313090860456622
13	28.15345411895707	21.718796431505748	25.98962150774029	24.138127941796892
14	21.9784604310168	20.783362249624865	27.620894268563095	29.61728305079524
15	23.483631792630426	28.762843104217122	24.11379810258855	23.639727000563894
16	23.326704685275722	27.980571661721793	25.46569289534914	23.227030757653345
17	22.25558531182466	27.492388105908272	27.086357802604727	23.16566877966234
18	22.313677279828433	26.902227208478706	28.167773670712197	22.616321840980667
19	23.066185883165403	26.57328285031424	28.318884654417133	22.041646612103225
20	23.28737220548415	26.111992161097263	28.15538941470102	22.44524621871756
21	23.50353812663014	25.859809481442962	28.12741163324915	22.509240758677745
22	23.07789125499085	25.876185978394368	28.092955383204444	22.952967383410336
23	22.904878910153815	25.871636497524104	27.942186455069397	23.281298137252694
24	22.64507713427623	26.1415354420935	28.104287374864846	23.109100048765423
25	22.937273270998826	25.892339227160292	28.105755272435463	23.06463222940542
26	22.836003811103897	26.12740147635192	28.04238350284931	22.99421120969487
27	22.908403580135456	26.21837203247414	27.888440676909042	22.98478371048136
28	22.876170345938878	26.260194093052107	27.93276359998242	22.930871961026604
29	22.98275465002305	26.255512797730525	27.912423391882292	22.849309160364133
30	23.162589543188858	26.231026311083593	27.835477961894085	22.77090618383346
31	23.088167231176577	25.996808290030867	27.84887163134848	23.066152847444076
32	22.931923955823844	26.15140840705355	27.81329229118637	23.10337534593624
33	22.91619634533562	26.035924598835468	27.85844871152928	23.18943034429963
34	23.081921744330355	26.06819499911353	27.893381833698438	22.956501422857674
35	23.226311726800976	26.059758060426674	27.781131267525218	22.932798945247132
36	23.10105863293125	26.201193220512152	27.675796810691278	23.021951335865317
37	23.167356229446927	25.945126703639502	27.760163617002497	23.127353449911073
38	22.99852646263514	26.02725987782749	27.729509172704365	23.244704486833008
39	23.184788638653924	26.266971801334943	27.59722223787208	22.95101732213905
40	23.389241176656167	26.047962214320158	27.72204269817877	22.840753910844906
41	23.138251125589836	26.066963018158162	27.588969294720506	23.205816561531492
42	23.19215388174637	26.24080979007112	27.44060728246206	23.12642904572045
43	23.11844534035877	25.9672400810454	27.568206016062614	23.346108562533214
44	23.162138197217608	26.286554901784786	27.344026097083646	23.207280803913964
45	23.302418771817486	26.302664761343213	27.14225893495924	23.252657531880054
46	23.416332925733755	26.176793563331664	27.050779196197606	23.35609431473698
47	23.318237450882677	26.31359151435899	26.93768150830136	23.430489526456977
48	23.285864988806715	26.46752970699166	26.878832728634265	23.367772575567354
49	23.408877784283145	26.29100117584806	26.797468340168972	23.502652699699823
50	23.43844239694332	26.459332767003236	26.811824431660707	23.290400404392738
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	611.0
1	461.5
2	312.0
3	461.5
4	611.0
5	693.0
6	775.0
7	737.5
8	700.0
9	989.0
10	1278.0
11	1904.5
12	2531.0
13	4827.5
14	7124.0
15	9847.0
16	12570.0
17	14696.5
18	16823.0
19	17765.0
20	18707.0
21	20094.5
22	21482.0
23	24271.0
24	27060.0
25	32090.0
26	37120.0
27	43186.0
28	49252.0
29	55365.0
30	61478.0
31	67342.0
32	73206.0
33	80120.5
34	87035.0
35	96516.5
36	105998.0
37	113818.5
38	121639.0
39	129475.0
40	137311.0
41	145665.0
42	154019.0
43	160302.0
44	166585.0
45	173803.5
46	181022.0
47	186011.5
48	191001.0
49	189573.0
50	188145.0
51	186178.5
52	184212.0
53	178297.5
54	172383.0
55	163199.0
56	154015.0
57	143726.5
58	133438.0
59	121060.0
60	108682.0
61	95636.5
62	82591.0
63	70789.5
64	58988.0
65	49937.0
66	40886.0
67	33014.0
68	25142.0
69	20245.5
70	15349.0
71	12212.0
72	9075.0
73	7501.5
74	5928.0
75	4681.5
76	3435.0
77	2697.0
78	1959.0
79	1494.5
80	1030.0
81	806.0
82	582.0
83	456.0
84	330.0
85	257.0
86	184.0
87	135.5
88	87.0
89	58.5
90	30.0
91	24.0
92	18.0
93	13.0
94	8.0
95	7.5
96	7.0
97	5.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016711983055175832
2	7.511003620303745E-4
3	4.1310519911670596E-4
4	0.0016148657783653052
5	0.03800567831873695
6	0.02501164205561147
7	0.027415163214108668
8	0.02140636031786567
9	0.028429148702849672
10	0.029706019318301313
11	0.03646592257657468
12	0.03620303744986405
13	0.03413751145428052
14	0.039582989079000735
15	0.03466328170770178
16	0.04059697456774174
17	0.042662500563325274
18	0.04589223212005589
19	0.0477324280070303
20	0.043676486052066275
21	0.044390031395995136
22	0.042775165617629826
23	0.0413105199116706
24	0.038982108789376434
25	0.03725457795670657
26	0.03995853926001593
27	0.04029653442292959
28	0.0393201039522901
29	0.037517463083417205
30	0.03440039658099116
31	0.03244753563971218
32	0.029330469137286125
33	0.02151902537217023
34	0.019228169267977588
35	0.01971638450329733
36	0.020767925010139854
37	0.016711983055175832
38	0.017237753308597094
39	0.01235560095539966
40	0.006159022968649071
41	0.005858582823836922
42	0.005407922606618696
43	0.00206552599558353
44	0.0032672865748321293
45	0.0016899758145683425
46	6.008802896242996E-4
47	1.1266505430455617E-4
48	1.1266505430455617E-4
49	3.7555018101518726E-5
50	7.511003620303745E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2662760.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.88765137259257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.64919390677245	43.37028054654485
2	13.952280465102046	16.43234055763126
3	5.404619238356558	9.547960005298433
4	2.516122715028419	5.926742290130186
5	1.4329950503660407	4.219285647230307
6	0.842096924789815	2.9753466077372956
7	0.5437513043371082	2.2414166070237274
8	0.3639917968792851	1.7147697629688696
9	0.25680044603270313	1.3610137624470084
>10	0.9958848769987712	9.509611167956015
>50	0.03150973110239799	1.2569912462772757
>100	0.01030488755970966	1.0393689930008998
>500	2.5637524270724117E-4	0.08167658728884478
>1k	1.92281431974035E-4	0.3231962184650627
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3901	0.14650212561402454	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.7555018101518726E-5	0.0	0.0	3.7555018101518726E-5	0.0
9	3.7555018101518726E-5	0.0	0.0	3.7555018101518726E-5	0.0
10	3.7555018101518726E-5	0.0	0.0	3.7555018101518726E-5	0.0
11	3.7555018101518726E-5	0.0	0.0	3.7555018101518726E-5	0.0
12	3.7555018101518726E-5	0.0	0.0	3.7555018101518726E-5	7.511003620303745E-5
13	3.7555018101518726E-5	0.0	0.0	3.7555018101518726E-5	1.1266505430455618E-4
14	3.7555018101518726E-5	0.0	0.0	3.7555018101518726E-5	1.1266505430455618E-4
15	3.7555018101518726E-5	0.0	0.0	7.511003620303745E-5	1.1266505430455618E-4
16	3.7555018101518726E-5	0.0	0.0	1.1266505430455618E-4	1.1266505430455618E-4
17	3.7555018101518726E-5	0.0	0.0	1.1266505430455618E-4	1.1266505430455618E-4
18	3.7555018101518726E-5	0.0	0.0	1.1266505430455618E-4	1.1266505430455618E-4
19	1.502200724060749E-4	0.0	0.0	1.8777509050759363E-4	1.1266505430455618E-4
20	1.502200724060749E-4	0.0	0.0	1.8777509050759363E-4	1.502200724060749E-4
21	1.502200724060749E-4	0.0	0.0	1.8777509050759363E-4	1.502200724060749E-4
22	1.502200724060749E-4	0.0	0.0	2.2533010860911236E-4	1.502200724060749E-4
23	1.502200724060749E-4	0.0	0.0	2.2533010860911236E-4	1.502200724060749E-4
24	2.2533010860911236E-4	0.0	0.0	3.379951629136685E-4	1.502200724060749E-4
25	2.2533010860911236E-4	0.0	0.0	3.7555018101518726E-4	1.502200724060749E-4
26	2.2533010860911236E-4	0.0	0.0	4.1310519911670596E-4	1.502200724060749E-4
27	2.2533010860911236E-4	0.0	0.0	4.506602172182247E-4	1.502200724060749E-4
28	3.004401448121498E-4	0.0	0.0	6.384353077258184E-4	1.502200724060749E-4
29	3.7555018101518726E-4	0.0	0.0	0.0012017605792485992	1.502200724060749E-4
30	4.506602172182247E-4	0.0	0.0	0.001915305923177455	1.502200724060749E-4
31	4.506602172182247E-4	0.0	0.0	0.0039057218825579473	1.502200724060749E-4
32	4.506602172182247E-4	0.0	0.0	0.005783472787633884	1.502200724060749E-4
33	4.506602172182247E-4	0.0	0.0	0.00826210398233412	1.502200724060749E-4
34	4.506602172182247E-4	0.0	0.0	0.011153840376151062	1.502200724060749E-4
35	4.882152353197434E-4	0.0	0.0	0.014421126950983191	1.502200724060749E-4
36	5.257702534212621E-4	0.0	0.0	0.017876188616322914	1.502200724060749E-4
37	5.257702534212621E-4	0.0	0.0	0.0234718863134492	1.502200724060749E-4
38	6.008802896242996E-4	0.0	0.0	0.03030689960792561	1.502200724060749E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5150	0.0	26.01809	1
GGTATCA	2150	0.0	24.151209	1
TATCAAC	7095	0.0	18.729115	2
ATCAACG	7335	0.0	18.116642	3
TCAACGC	7385	0.0	18.02445	4
CAACGCA	7545	0.0	17.731035	5
AACGCAG	7715	0.0	17.252504	6
ACGCAGA	9000	0.0	14.715618	7
CGCAGAG	9175	0.0	14.435481	8
TACACCG	215	3.5415724E-9	14.327795	5
GAGTACT	6355	0.0	14.1952095	12
TAACACG	140	2.7550781E-5	14.145042	5
AGAGTAC	9070	0.0	13.681351	11
GTATTAC	405	0.0	13.581581	1
AGTACTT	6605	0.0	13.557982	13
CAGAGTA	9485	0.0	13.360852	10
GGCGAAT	330	0.0	13.331637	32
GTACATG	5760	0.0	13.063783	1
GCAGAGT	9910	0.0	13.054027	9
CGCGTCC	305	1.8189894E-11	12.985126	18
>>END_MODULE