FastQCFastQC Report
Mon 6 Feb 2017
SRR2077381_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077381_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604540
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86240.3311141314781113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39780.15273330415351655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37840.14528477197508965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17950.027.9165631
GTATCAA47950.025.3738541
TATCAAC63050.018.9210912
ATCAACG65000.018.1298833
TCAACGC64800.018.0273064
CAACGCA66450.017.5164415
AACGCAG68900.017.0051166
TCGCGTA750.002444413715.99863327
ACGCAGA79550.014.6279677
CGCAGAG79400.014.6052398
GTTCGAC1101.271534E-414.541141511
TCTATAC4200.013.814713
AGAGTAC78450.013.66070211
GTACCGA2352.3646862E-1013.6129826
GAGTACT43800.013.51197212
CGAATCG1508.700343E-613.33680337
TAGTACT3450.013.3352664
ACCGATC3000.013.3293798
CAGAGTA82450.013.11921210
GCAGAGT85750.012.84759
Produced by FastQC (version 0.11.5)