FastQCFastQC Report
Mon 6 Feb 2017
SRR2077381_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077381_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604540
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28110.10792692759565989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25850.042.240551
GTATCAA62150.040.046351
TATCAAC86550.028.3851472
ATCAACG88000.027.6806473
TCAACGC88950.027.4234394
CAACGCA90350.026.8829365
AACGCAG93150.026.147746
ACGCAGA105500.022.9256427
CGCAGAG108950.022.1282678
GAGTACT77050.021.97934212
AGAGTAC110050.021.02995311
AGTACTT81550.020.89338513
CAGAGTA116700.020.097210
GTACTTT85550.019.83128514
GCAGAGT119500.019.7706159
GTACATG71600.019.4622291
TACATGG73600.018.6435812
CGTCGTA1150.001112825117.9949610
TACTTTT94900.017.91374815
ACATGGG76300.017.7110233
Produced by FastQC (version 0.11.5)