FastQCFastQC Report
Mon 6 Feb 2017
SRR2077380_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077380_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432464
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62820.2582566484026074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28610.11761736247689586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28050.11531517013201428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.026.6890661
GTATCAA41250.024.0098671
TATCAAC55400.017.558552
ATCAACG55750.017.329913
TCAACGC56100.017.2556744
CAACGCA57200.016.9217455
AACGCAG58500.016.5734186
CGGCCTA800.00374363415.00715339
CGAATAG800.003762633814.99573620
GTATAAA7600.014.50894451
ACGCAGA67400.014.2069827
CGCAGAG68500.013.8329248
GAGTACT32200.013.65874712
TAAGGCG2201.2787496E-913.635295
GTATAGA4200.013.3658151
CGAATCG1050.001467119813.33914237
GCGATAC1354.8208814E-513.32817414
TGCGATA1354.8227434E-513.32762513
AGAGTAC67350.013.17893911
ACTATAC3250.012.9249853
Produced by FastQC (version 0.11.5)