FastQCFastQC Report
Mon 6 Feb 2017
SRR2077380_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077380_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432464
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28990.11917956442520836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25600.041.8595621
GTATCAA58400.038.0646061
TATCAAC82900.026.681342
TCAACGC83250.026.4409524
ATCAACG84500.026.1728843
CAACGCA86250.025.520745
AACGCAG88850.024.7338946
ACGCAGA102800.021.211427
CGCAGAG106250.020.4493148
CGCGAGT850.00412689420.2895212
GTGGTAT10550.020.0518071
AGAGTAC103850.019.49630411
GAGTACT69650.018.8184212
CAGAGTA110450.018.64204810
GCAGAGT112950.018.4427959
AGTACTT73400.018.3269113
GTACATG70950.018.0364111
CCCGCGA1402.0831315E-417.25070421
GTACTTT75650.017.18379814
TACATGG74200.016.9139942
Produced by FastQC (version 0.11.5)