FastQCFastQC Report
Mon 6 Feb 2017
SRR2077379_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077379_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3000045
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46300.15433101836805782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18000.025.170831
GTATCAA40500.022.8195721
TATCAAC53000.016.8806742
CAACGCA55550.016.4878065
CGATCGT852.9661728E-416.47039625
TCAACGC55450.016.3768334
ATCAACG55100.016.3727743
AACGCAG57200.016.0081946
CGAATCG1151.8452165E-413.91775437
ACCGTTA1303.3595355E-513.8503835
GTACATG84900.013.4122691
ACGCAGA66850.013.3683877
GTACTAG3050.013.1459431
CGCAGAG68050.013.044518
TACATGG86300.012.8950232
GTATATA6600.012.4537891
TATACTA4900.012.243535
ACATGGG90050.012.1062643
TAACCGT1501.2856473E-412.00186133
CGTATTA1501.291656E-411.99585928
Produced by FastQC (version 0.11.5)