##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077379_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3000045
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.317606569234794	31.0	31.0	34.0	26.0	34.0
2	30.442469362959557	31.0	31.0	34.0	26.0	34.0
3	30.528427740250564	33.0	31.0	34.0	26.0	34.0
4	33.96958778951649	37.0	35.0	37.0	30.0	37.0
5	34.004185270554274	37.0	35.0	37.0	30.0	37.0
6	34.1275460868087	37.0	35.0	37.0	30.0	37.0
7	34.07125993110103	37.0	35.0	37.0	30.0	37.0
8	34.03357716300922	37.0	35.0	37.0	30.0	37.0
9	35.569930784371564	39.0	37.0	39.0	30.0	39.0
10	35.49207828549238	39.0	37.0	39.0	30.0	39.0
11	35.52787274857544	39.0	37.0	39.0	30.0	39.0
12	35.44663496714216	39.0	37.0	39.0	30.0	39.0
13	35.41588342841524	39.0	37.0	39.0	29.0	39.0
14	36.49656955145673	40.0	37.0	41.0	27.0	41.0
15	36.62783491580959	40.0	37.0	41.0	29.0	41.0
16	36.599912334648316	40.0	37.0	41.0	29.0	41.0
17	36.61124616464086	40.0	37.0	41.0	29.0	41.0
18	36.571247764616864	40.0	37.0	41.0	29.0	41.0
19	36.656893479931135	40.0	37.0	41.0	29.0	41.0
20	36.64957225641616	40.0	37.0	41.0	29.0	41.0
21	36.66084408733869	40.0	37.0	41.0	29.0	41.0
22	36.610764505199086	40.0	37.0	41.0	29.0	41.0
23	36.56266089341993	40.0	37.0	41.0	29.0	41.0
24	36.58479322810158	40.0	37.0	41.0	29.0	41.0
25	36.52266749332093	40.0	37.0	41.0	29.0	41.0
26	36.5487491020968	40.0	37.0	41.0	29.0	41.0
27	36.52534178653987	40.0	37.0	41.0	29.0	41.0
28	36.45690281312447	40.0	37.0	41.0	29.0	41.0
29	36.41978570321445	40.0	37.0	41.0	29.0	41.0
30	36.33929924384468	40.0	37.0	41.0	28.0	41.0
31	36.310584674563216	40.0	37.0	41.0	28.0	41.0
32	36.28642903689778	40.0	37.0	41.0	29.0	41.0
33	36.243951007401556	40.0	37.0	41.0	28.0	41.0
34	36.18744852160551	40.0	37.0	41.0	28.0	41.0
35	36.16501252481213	40.0	37.0	41.0	28.0	41.0
36	36.10584741228882	40.0	37.0	41.0	28.0	41.0
37	36.021657675134875	40.0	36.0	41.0	27.0	41.0
38	35.99173112403314	40.0	36.0	41.0	27.0	41.0
39	35.909235694797914	40.0	36.0	41.0	27.0	41.0
40	35.88386374204387	40.0	36.0	41.0	27.0	41.0
41	35.75134672979905	40.0	36.0	41.0	27.0	41.0
42	35.704747428788565	40.0	36.0	41.0	27.0	41.0
43	35.60522658826784	40.0	36.0	41.0	26.0	41.0
44	35.52216750082082	40.0	36.0	41.0	26.0	41.0
45	35.42392730775705	40.0	35.0	41.0	26.0	41.0
46	35.319030881203446	40.0	35.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.25502875847558215
1101	2	0.36022087566862027
1101	3	0.5104005927500523
1101	4	0.49022852048256027
1101	5	0.4684076411443385
1101	6	0.41175576403387026
1101	7	0.4926956997751617
1101	8	0.561639600267732
1101	9	0.5699559803906027
1101	10	0.6032050483675278
1101	11	0.6034158384797266
1101	12	0.5898868765681726
1101	13	0.7019209037677925
1101	14	0.9604665478312029
1101	15	0.8265048160093187
1101	16	0.7681843063558773
1101	17	0.7108059260500141
1101	18	0.6411514048856333
1101	19	0.6842259130726163
1101	20	0.6326183756467998
1101	21	0.6394506569197702
1101	22	0.7736027267262244
1101	23	0.84257276703066
1101	24	0.7049954375069021
1101	25	0.7860868961271734
1101	26	0.7882161747019936
1101	27	0.7664107513267595
1101	28	0.6771686664557777
1101	29	0.6883221067975995
1101	30	0.7044424381259233
1101	31	0.6624471490654784
1101	32	0.7102309093083576
1101	33	0.7032994555639647
1101	34	0.6162536204804923
1101	35	0.6741464057183109
1101	36	0.6329542003558402
1101	37	0.7421091336540186
1101	38	0.5956705705121479
1101	39	0.7395384230688649
1101	40	0.6453571514237311
1101	41	0.6414989973009853
1101	42	0.5463840245911555
1101	43	0.6077741609540439
1101	44	0.6569244999613275
1101	45	0.5664135942189432
1101	46	0.5645617940745282
1102	1	0.5716945111654823
1102	2	0.6503868453214032
1102	3	0.6526063861317581
1102	4	0.6440798980717233
1102	5	0.6536437202307326
1102	6	0.7334929754318011
1102	7	0.7628372356588002
1102	8	0.7687674622061493
1102	9	0.8650252221951504
1102	10	0.8744045911492648
1102	11	0.8568574059428329
1102	12	0.8757723760060472
1102	13	0.9376349741638776
1102	14	1.2186593298915724
1102	15	1.1125905931552325
1102	16	1.1350908427951296
1102	17	1.0435110254953273
1102	18	1.1059176005446645
1102	19	1.1105380657637625
1102	20	1.0509498982679446
1102	21	1.0364426759560033
1102	22	1.063121144767031
1102	23	1.0807762654222586
1102	24	1.0512303778857728
1102	25	1.0320751406432862
1102	26	1.0773028360509116
1102	27	1.00816429862261
1102	28	1.1269064700661602
1102	29	1.156277228573238
1102	30	1.1282160527920553
1102	31	1.0181325165125017
1102	32	1.0573775276073007
1102	33	1.0865319344346887
1102	34	1.0992881909379264
1102	35	1.0368396575775662
1102	36	1.0145476389444426
1102	37	1.0496123341485202
1102	38	0.9783791905292176
1102	39	1.1653065834627796
1102	40	1.0304528637010222
1102	41	1.108392218971204
1102	42	1.1133241093540676
1102	43	0.9996167459013279
1102	44	1.0480233154585576
1102	45	1.0481635731121983
1102	46	0.9470231954267163
1103	1	0.5371319493259001
1103	2	0.49896056890168694
1103	3	0.508366784306844
1103	4	0.5435727190106476
1103	5	0.5704703607601758
1103	6	0.5394998463884022
1103	7	0.5892322426599961
1103	8	0.6251597823376045
1103	9	0.7625920931776733
1103	10	0.8031451987144393
1103	11	0.7181265350406605
1103	12	0.7279083078563175
1103	13	0.7758339031741457
1103	14	0.9955125302579759
1103	15	0.910739126079001
1103	16	0.9565176786193987
1103	17	0.8357010899953181
1103	18	0.7885388881989073
1103	19	0.7596020998740514
1103	20	0.677500437013407
1103	21	0.8145198167637417
1103	22	0.820587899613713
1103	23	0.7670692124107958
1103	24	0.837257110943753
1103	25	0.8293081075846374
1103	26	0.8220544766289493
1103	27	0.8024074750210133
1103	28	0.8540000416007203
1103	29	0.9037754591053613
1103	30	0.8723828564300433
1103	31	0.9065796353954738
1103	32	0.8168028463436983
1103	33	0.8528300456559776
1103	34	0.996463856710335
1103	35	0.9000845754736133
1103	36	0.9197772723530306
1103	37	0.931232935614247
1103	38	0.9668921204418695
1103	39	0.9819941936022971
1103	40	0.9476017558100267
1103	41	1.009147857887875
1103	42	1.0273650124101223
1103	43	1.0329115220779528
1103	44	1.0004642631805822
1103	45	0.9580804335005837
1103	46	0.9319377393618922
1104	1	0.4511941089010989
1104	2	0.37206677758291207
1104	3	0.4620163154027921
1104	4	0.47912924195901496
1104	5	0.4720839573828073
1104	6	0.47310912643757064
1104	7	0.5417481102998067
1104	8	0.5676604760875179
1104	9	0.5689186962507691
1104	10	0.7215252869348276
1104	11	0.6535375842094453
1104	12	0.6285987115072302
1104	13	0.7319879704875945
1104	14	0.9511881134092448
1104	15	0.7952091251220352
1104	16	0.8138116015369548
1104	17	0.6975411336781079
1104	18	0.6790569166273315
1104	19	0.6860100890375875
1104	20	0.7778665555811557
1104	21	0.7398648726463151
1104	22	0.796813335081211
1104	23	0.7679672823578869
1104	24	0.7613952191945899
1104	25	0.5734277561458399
1104	26	0.6738723620125029
1104	27	0.7627301401381814
1104	28	0.7390686149485859
1104	29	0.857883355094792
1104	30	0.9189046518527348
1104	31	0.8561596534798781
1104	32	0.8797761052294817
1104	33	0.7882851904240198
1104	34	0.8348911699623471
1104	35	0.7709400321841358
1104	36	0.7557031767350111
1104	37	0.6605427696417507
1104	38	0.7657316973159283
1104	39	0.746907054234974
1104	40	0.7795130321284844
1104	41	0.8554118869079943
1104	42	0.8153650466024516
1104	43	0.7847102680950115
1104	44	0.7671293765980067
1104	45	0.7881163959115369
1104	46	0.7859478431960838
1105	1	0.1628635597332888
1105	2	0.013876734226183629
1105	3	0.2557486991373601
1105	4	0.3185705209717611
1105	5	0.391175277089566
1105	6	0.4337446233642268
1105	7	0.3492300593448121
1105	8	0.3822150664861894
1105	9	0.5353582020683092
1105	10	0.4260109450233145
1105	11	0.40935181656843156
1105	12	0.5026266749216646
1105	13	0.5161617958879745
1105	14	0.8554400307322894
1105	15	0.6116774257602557
1105	16	0.5832530250618788
1105	17	0.44711705192234064
1105	18	0.3180332046382617
1105	19	0.453008488027109
1105	20	0.5622328589073078
1105	21	0.4737292421227579
1105	22	0.37432090582837674
1105	23	0.5512508204834248
1105	24	0.40431023281102085
1105	25	0.49513520753209406
1105	26	0.5928376179020631
1105	27	0.5876425642193226
1105	28	0.7665017926699775
1105	29	0.5684156180540754
1105	30	0.682893815284018
1105	31	0.6581807331949534
1105	32	0.6350116739202747
1105	33	0.6734916322235875
1105	34	0.567869095573009
1105	35	0.6105159842503696
1105	36	0.6425519840944318
1105	37	0.6604202417608889
1105	38	0.6166512406205413
1105	39	0.6134597407625364
1105	40	0.4667679187156324
1105	41	0.5355972068687223
1105	42	0.5773763917310575
1105	43	0.6472235316996802
1105	44	0.642854589453691
1105	45	0.5323928680063901
1105	46	0.620486777083606
1106	1	0.09611453710913764
1106	2	0.2291325367117132
1106	3	0.26571348040798526
1106	4	0.3759558067142521
1106	5	0.3662574029302519
1106	6	0.27559098539348525
1106	7	0.3275007766049072
1106	8	0.3745885304307137
1106	9	0.24587814916109352
1106	10	0.4039434681593619
1106	11	0.3888806780239449
1106	12	0.25086447151765157
1106	13	0.25643563797903823
1106	14	0.6051363024161418
1106	15	0.47733565339758144
1106	16	0.4443530267890452
1106	17	0.4823967493223691
1106	18	0.48904804453437123
1106	19	0.3998263760761631
1106	20	0.408778774758197
1106	21	0.4005392244963275
1106	22	0.4211792013527429
1106	23	0.4905650910447221
1106	24	0.4980198945000254
1106	25	0.5112978356748314
1106	26	0.5100973395093504
1106	27	0.47760329750278174
1106	28	0.4884201228225251
1106	29	0.5438629314947647
1106	30	0.6201401890828464
1106	31	0.554704397527253
1106	32	0.4996861910343995
1106	33	0.525200903593138
1106	34	0.5914762347992593
1106	35	0.45218657841607524
1106	36	0.33775178907949055
1106	37	0.3249027638989048
1106	38	0.4011985719224356
1106	39	0.5007832321297414
1106	40	0.4725431263559656
1106	41	0.5650110978355585
1106	42	0.4150690162963784
1106	43	0.5313051686585268
1106	44	0.5405621917783563
1106	45	0.4789494054280894
1106	46	0.4376339534781124
1107	1	0.31086401960178733
1107	2	0.2212960864436191
1107	3	0.29963920468873795
1107	4	0.2959323805981029
1107	5	0.30627024986613094
1107	6	0.2299521528681936
1107	7	0.35350837127830914
1107	8	0.3498024633205006
1107	9	0.4234923307815279
1107	10	0.36294982193896885
1107	11	0.43639030858498273
1107	12	0.4189701846512861
1107	13	0.4658967846275175
1107	14	0.6121224948449395
1107	15	0.5321725428175554
1107	16	0.5250894412898148
1107	17	0.4824464191908362
1107	18	0.5468984399943224
1107	19	0.49749023157878725
1107	20	0.553977196099261
1107	21	0.382670012685395
1107	22	0.32383559107277193
1107	23	0.4234647111003298
1107	24	0.5197775546097176
1107	25	0.389453484084612
1107	26	0.34845543226867903
1107	27	0.4340485925897184
1107	28	0.4587340479306974
1107	29	0.508047645005739
1107	30	0.39792552604599507
1107	31	0.44309921337772096
1107	32	0.5007804834597778
1107	33	0.5617852702980457
1107	34	0.5314517540764072
1107	35	0.4743023632945693
1107	36	0.5946525267573151
1107	37	0.5870686581489437
1107	38	0.5956115044309698
1107	39	0.5914460945862032
1107	40	0.5874089115982031
1107	41	0.6635613347181817
1107	42	0.5780043469805562
1107	43	0.47505514639488666
1107	44	0.4973120785536338
1107	45	0.480279955843983
1107	46	0.5136087334267998
1108	1	0.23533953682707676
1108	2	0.2186214734086036
1108	3	0.14186741913470158
1108	4	0.09860227050899084
1108	5	0.191577964283006
1108	6	0.11118286077810069
1108	7	0.15289496993199236
1108	8	0.24681646968750215
1108	9	0.07083925980045791
1108	10	0.32594083245137284
1108	11	0.19205330561459988
1108	12	0.006039393971505547
1108	13	0.07624696396223385
1108	14	0.1937619833881925
1108	15	0.2221545949458701
1108	16	0.16444795463466022
1108	17	0.13603867188489005
1108	18	0.25111725542327434
1108	19	0.17247449263162196
1108	20	0.09988437864627997
1108	21	0.02739949575097711
1108	22	0.14017964532187932
1108	23	0.13966687002885436
1108	24	0.2167136870276849
1108	25	0.1985118313713201
1108	26	0.10810802920936169
1108	27	0.10001111056782719
1108	28	0.1201941902349759
1108	29	0.19694395457542413
1108	30	-0.10351099487773041
1108	31	0.037449377736678
1108	32	0.22077698143603186
1108	33	0.17758173014371437
1108	34	0.3042666035786681
1108	35	0.20266800682887265
1108	36	0.21410955179966606
1108	37	0.2571411581043037
1108	38	0.13066039740554913
1108	39	0.15259039236420335
1108	40	0.2191764428845815
1108	41	0.18422428278858405
1108	42	0.1541333320445375
1108	43	0.24039637195343744
1108	44	0.19972473099678467
1108	45	0.11642228085367634
1108	46	0.04341222266354805
1109	1	0.10866146127591136
1109	2	0.036703878924548405
1109	3	0.1435415489254197
1109	4	0.12672990571329024
1109	5	0.13166553761778488
1109	6	0.09284691196926786
1109	7	0.028464850476908055
1109	8	0.08707855837015899
1109	9	0.1401611977776156
1109	10	0.14560999734808888
1109	11	0.09236423153802065
1109	12	0.06385887655829947
1109	13	0.16225946864077656
1109	14	0.2129541622145652
1109	15	0.1710429051232225
1109	16	0.06898929944001253
1109	17	0.10575045099445646
1109	18	0.021222595953673817
1109	19	0.08577373459466742
1109	20	-0.0831470476446583
1109	21	-0.023450646521865792
1109	22	-0.0752487289797159
1109	23	-0.009969894537768198
1109	24	0.04880861525994362
1109	25	-2.6543383338406557E-4
1109	26	0.10305069167323211
1109	27	0.0740807002538375
1109	28	-0.012929566850480967
1109	29	0.09544715853290597
1109	30	0.1416763047340055
1109	31	0.24790327547158597
1109	32	0.1464794395320581
1109	33	0.1212537450068325
1109	34	0.20618119928477796
1109	35	0.2808115378109832
1109	36	0.1868764768572717
1109	37	0.20754069453381874
1109	38	0.2739957825343211
1109	39	0.33553106616480477
1109	40	0.2862628257270572
1109	41	0.3039707845901063
1109	42	0.27183536625314986
1109	43	0.2575801587110149
1109	44	0.2664390849968825
1109	45	0.2452321944076985
1109	46	0.12219327579828132
1110	1	0.2607540193130582
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2216	11	-1.9314112118733107
2216	12	-2.2160501935594468
2216	13	-2.1644591692248554
2216	14	-3.8733740213171117
2216	15	-2.856309008505221
2216	16	-2.459984249664494
2216	17	-2.4773699502642117
2216	18	-2.724971243211634
2216	19	-2.443068703556449
2216	20	-2.258949091792573
2216	21	-2.453674974405722
2216	22	-2.3958130384094574
2216	23	-2.48422088624919
2216	24	-2.4277875866627525
2216	25	-2.365129472760522
2216	26	-2.2530289133772
2216	27	-2.5567640880364806
2216	28	-2.51676727317772
2216	29	-2.7245631331840343
2216	30	-2.484350895366937
2216	31	-2.480610300919764
2216	32	-2.3987715837248587
2216	33	-2.282851900847696
2216	34	-2.248133041139198
2216	35	-2.2651246141121035
2216	36	-2.589536958368001
2216	37	-2.4545035716785932
2216	38	-2.511518527289738
2216	39	-2.5069120139370895
2216	40	-2.530891613118058
2216	41	-2.6827456056591785
2216	42	-2.559034461271864
2216	43	-2.6214921672170135
2216	44	-2.5919324972461624
2216	45	-2.626245554190497
2216	46	-2.780518453604479
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	92200.0
3	3011.0
4	8806.0
5	6828.0
6	5450.0
7	4654.0
8	3985.0
9	3578.0
10	3486.0
11	3472.0
12	3443.0
13	3518.0
14	3604.0
15	3848.0
16	4208.0
17	4552.0
18	4948.0
19	6135.0
20	8333.0
21	10971.0
22	14069.0
23	16443.0
24	18214.0
25	19106.0
26	21424.0
27	24174.0
28	28252.0
29	31290.0
30	35309.0
31	42573.0
32	52841.0
33	66299.0
34	86314.0
35	117468.0
36	161856.0
37	235925.0
38	428659.0
39	1410799.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.15674234111005	17.968945671622652	13.64457567789416	24.229736309373138
2	15.545926614395588	20.155612671117414	38.65565218957253	25.642808524914468
3	18.277767012019588	25.315482112702036	29.399847708066435	27.006903167211938
4	12.899201155794463	16.791136241073325	37.703125307449795	32.606537295682415
5	14.2617196774271	37.57112636417232	34.17099938377158	13.996154574628997
6	31.929621055684166	36.68614970775438	17.83579912967972	13.548430106881732
7	28.19534373651062	31.916787914847944	21.97997030044549	17.907898048195943
8	26.89812986138541	33.81819272710909	20.198597021044684	19.08508039046081
9	27.098126861430412	15.585032891173297	19.02831457528137	38.28852567211492
10	14.897209875185204	28.635437135109644	33.60709589356159	22.860257096143556
11	34.30531875355203	23.17958563954874	23.885441718374224	18.629653888525006
12	23.703477781166615	24.53856525485451	30.055182505595752	21.702774458383125
13	27.85101556809981	22.087535353636362	25.926444436666785	24.135004641597043
14	22.750992068452305	20.993951757390306	26.807064560698258	29.44799161345913
15	23.76793391559185	28.649932234282055	23.615902710695384	23.966231139430715
16	23.808176210690174	27.981946937462602	24.995025074623882	23.21485177722334
17	22.758785974972977	27.713158204382143	26.339108484328694	23.188947336316186
18	22.84459439865041	27.58351841500093	26.932357608781523	22.639529577567135
19	23.880145278450364	27.10227757570586	26.832013791852887	22.185563353990894
20	24.152970200164468	26.525783646107325	26.68148452841233	22.639761625315877
21	24.17736704788146	26.3990885245224	26.64685758629	22.77668684130613
22	24.088705336086626	26.081175449034934	26.608167544153506	23.221951670724938
23	23.855331165805232	26.19866815020343	26.482097893426683	23.463902790564653
24	23.53011567876609	26.356918859532165	26.549883467909673	23.563081993792064
25	23.65829496144367	26.262705723217067	26.56416977719526	23.514829538144003
26	23.506794283508587	26.60297157820843	26.57420512249577	23.31602901578722
27	23.566283506508924	26.603457910396806	26.38287075379726	23.447387829297014
28	23.662571261811134	26.40474475413404	26.489310603330836	23.443373380723987
29	23.723348049926418	26.591733707998127	26.383901413826266	23.301016828249193
30	23.758112886919413	26.565469259946045	26.344203404587518	23.332214448547024
31	23.835301552931263	26.209431720757703	26.433998088002546	23.52126863830848
32	23.66873431885977	26.528080970099875	26.3530821950913	23.450102515949055
33	23.780925206168043	26.39860932406216	26.213108087387248	23.60735738238255
34	23.90031070403915	26.28760840557594	26.453877233737373	23.358203656647536
35	23.857897293007166	26.408888619256654	26.332854550927326	23.400359536808857
36	23.7608179910005	26.5083167864544	26.294816200436472	23.436049022108634
37	23.85043131223125	26.22732389736779	26.443738397139278	23.478506393261682
38	23.84948857639484	26.310062520293926	26.304528570847552	23.535920332463682
39	23.891069425782316	26.51228265360946	26.184551793782017	23.412096126826206
40	23.98321889447933	26.26058320475909	26.42250673764587	23.333691163115706
41	23.78134113509807	26.310277570993655	26.315013594388283	23.593367699519995
42	23.748601274403697	26.419545970753084	26.326125275784495	23.50572747905872
43	23.798538497880966	26.226917925623233	26.34854861710105	23.625994959394752
44	23.790945604138756	26.38690407520501	26.20111884586428	23.62103147479196
45	23.88612631692661	26.4354908512819	26.059259446962507	23.61912338482898
46	23.887335382916532	26.239043726404983	26.10147870726224	23.772142183416246
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	810.0
1	743.0
2	676.0
3	859.5
4	1043.0
5	1022.0
6	1001.0
7	1001.0
8	1045.0
9	1089.0
10	1225.5
11	1362.0
12	1807.5
13	2253.0
14	4071.0
15	5889.0
16	8369.5
17	10850.0
18	11683.0
19	12516.0
20	12516.0
21	14805.0
22	17094.0
23	22171.0
24	27248.0
25	34308.5
26	41369.0
27	48493.0
28	55617.0
29	64685.0
30	73753.0
31	73753.0
32	83141.5
33	92530.0
34	104448.0
35	116366.0
36	127546.0
37	138726.0
38	148742.0
39	158758.0
40	169685.5
41	180613.0
42	190607.0
43	200601.0
44	200601.0
45	208027.5
46	215454.0
47	222313.0
48	229172.0
49	232880.5
50	236589.0
51	234818.5
52	233048.0
53	225178.5
54	217309.0
55	205309.0
56	193309.0
57	193309.0
58	178513.0
59	163717.0
60	145698.5
61	127680.0
62	110104.0
63	92528.0
64	77402.5
65	62277.0
66	50521.0
67	38765.0
68	30760.5
69	22756.0
70	22756.0
71	17745.0
72	12734.0
73	9925.5
74	7117.0
75	5395.0
76	3673.0
77	2792.0
78	1911.0
79	1411.0
80	911.0
81	911.0
82	677.0
83	443.0
84	324.5
85	206.0
86	167.5
87	129.0
88	90.5
89	52.0
90	42.5
91	33.0
92	26.5
93	20.0
94	20.0
95	15.5
96	11.0
97	14.5
98	18.0
99	18.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18503055787496522
2	0.04819927701084484
3	0.06186573868058646
4	0.052932539345243154
5	0.038566088175344036
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	9.999850002249967E-5
16	0.0
17	2.6666266672666577E-4
18	5.999910001349979E-4
19	4.333268334308318E-4
20	0.0020666356671316596
21	0.0024666296672216583
22	0.0
23	2.3332983338583257E-4
24	4.333268334308318E-4
25	6.333238334758312E-4
26	7.66655166839164E-4
27	0.02589961150582741
28	5.333253334533315E-4
29	9.999850002249966E-4
30	0.0011333163335883294
31	0.001366646166974162
32	7.999880001799974E-4
33	0.002166634167154159
34	0.002799958000629991
35	0.0024666296672216583
36	0.032999505007424886
37	0.008199877001844973
38	0.01263314383617579
39	0.05159922601160982
40	0.01603309283694078
41	0.058299125513117306
42	0.05933244334668313
43	0.02673293233934824
44	0.029432891839955736
45	0.027566253172869072
46	0.024532965338853248
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	3000045.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.52586263886085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.75043608531888	46.4538635197268
2	12.921615453741381	15.64183844050665
3	4.458406005027108	8.09546608345629
4	1.9740691544730002	4.779289539329799
5	1.08982462368442	3.298128773678571
6	0.6329769447385312	2.2986885366486094
7	0.4320138736654442	1.8303608662889805
8	0.31392269209437434	1.5200353392740407
9	0.24015525580350824	1.308204362228727
>10	1.1349686317641996	11.84791053325971
>50	0.04140485658568449	1.674680176689104
>100	0.00998526557977829	0.9346596644871051
>500	5.528938091410976E-5	0.020614054843505585
>1k	1.6586814271781945E-4	0.2962601095821234
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4630	0.15433101836805782	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.333283334083322E-5	0.0	0.0	0.0	0.0
7	3.333283334083322E-5	0.0	0.0	0.0	0.0
8	3.333283334083322E-5	0.0	0.0	0.0	0.0
9	3.333283334083322E-5	0.0	0.0	3.333283334083322E-5	0.0
10	3.333283334083322E-5	0.0	0.0	6.666566668166644E-5	0.0
11	3.333283334083322E-5	0.0	0.0	9.999850002249966E-5	0.0
12	6.666566668166644E-5	0.0	0.0	9.999850002249966E-5	0.0
13	6.666566668166644E-5	3.333283334083322E-5	0.0	9.999850002249966E-5	0.0
14	6.666566668166644E-5	3.333283334083322E-5	0.0	9.999850002249966E-5	0.0
15	6.666566668166644E-5	3.333283334083322E-5	0.0	9.999850002249966E-5	3.333283334083322E-5
16	6.666566668166644E-5	3.333283334083322E-5	0.0	9.999850002249966E-5	3.333283334083322E-5
17	9.999850002249966E-5	3.333283334083322E-5	0.0	9.999850002249966E-5	3.333283334083322E-5
18	9.999850002249966E-5	3.333283334083322E-5	0.0	9.999850002249966E-5	3.333283334083322E-5
19	1.3333133336333288E-4	3.333283334083322E-5	0.0	9.999850002249966E-5	3.333283334083322E-5
20	1.3333133336333288E-4	3.333283334083322E-5	0.0	9.999850002249966E-5	3.333283334083322E-5
21	1.3333133336333288E-4	3.333283334083322E-5	0.0	9.999850002249966E-5	3.333283334083322E-5
22	1.3333133336333288E-4	3.333283334083322E-5	0.0	1.3333133336333288E-4	3.333283334083322E-5
23	1.3333133336333288E-4	3.333283334083322E-5	0.0	2.3332983338583254E-4	3.333283334083322E-5
24	1.666641667041661E-4	6.666566668166644E-5	0.0	2.9999550006749897E-4	3.333283334083322E-5
25	1.666641667041661E-4	6.666566668166644E-5	0.0	3.333283334083322E-4	3.333283334083322E-5
26	1.666641667041661E-4	6.666566668166644E-5	0.0	4.333268334308319E-4	3.333283334083322E-5
27	1.666641667041661E-4	6.666566668166644E-5	0.0	6.333238334758312E-4	3.333283334083322E-5
28	1.666641667041661E-4	6.666566668166644E-5	0.0	0.001066650666906663	3.333283334083322E-5
29	1.666641667041661E-4	6.666566668166644E-5	0.0	0.0016333088337008278	3.333283334083322E-5
30	1.999970000449993E-4	6.666566668166644E-5	0.0	0.0024999625005624917	3.333283334083322E-5
31	1.999970000449993E-4	6.666566668166644E-5	0.0	0.00383327583419582	3.333283334083322E-5
32	1.999970000449993E-4	6.666566668166644E-5	0.0	0.005733247334623314	3.333283334083322E-5
33	1.999970000449993E-4	6.666566668166644E-5	0.0	0.008099878501822472	6.666566668166644E-5
34	1.999970000449993E-4	6.666566668166644E-5	0.0	0.010599841002384964	6.666566668166644E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1800	0.0	25.17083	1
GTATCAA	4050	0.0	22.819572	1
TATCAAC	5300	0.0	16.880674	2
CAACGCA	5555	0.0	16.487806	5
CGATCGT	85	2.9661728E-4	16.470396	25
TCAACGC	5545	0.0	16.376833	4
ATCAACG	5510	0.0	16.372774	3
AACGCAG	5720	0.0	16.008194	6
CGAATCG	115	1.8452165E-4	13.917754	37
ACCGTTA	130	3.3595355E-5	13.85038	35
GTACATG	8490	0.0	13.412269	1
ACGCAGA	6685	0.0	13.368387	7
GTACTAG	305	0.0	13.145943	1
CGCAGAG	6805	0.0	13.04451	8
TACATGG	8630	0.0	12.895023	2
GTATATA	660	0.0	12.453789	1
TATACTA	490	0.0	12.24353	5
ACATGGG	9005	0.0	12.106264	3
TAACCGT	150	1.2856473E-4	12.001861	33
CGTATTA	150	1.291656E-4	11.995859	28
>>END_MODULE