FastQCFastQC Report
Mon 6 Feb 2017
SRR2077379_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077379_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3000045
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25300.041.521711
GTATCAA57050.037.7996521
TCAACGC78900.027.1409114
ATCAACG79050.027.0451243
CAACGCA80150.026.7595165
TATCAAC80450.026.6206742
AACGCAG83950.025.6299346
ACGCAGA96800.022.1341937
CGCAGAG100350.021.172558
GTACATG89900.020.6322781
AGAGTAC101550.019.63300111
TACATGG93400.019.3677982
GAGTACT59900.019.0607512
TATACTG9650.019.0300395
CAGAGTA109100.018.81120310
ACATGGG95250.018.5164833
GCAGAGT113350.018.5014769
AGTACTT65050.017.49868613
CGTTCGG1402.0917231E-417.24163615
GTACTTT66200.017.24163614
Produced by FastQC (version 0.11.5)