FastQCFastQC Report
Mon 6 Feb 2017
SRR2077378_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077378_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2214775
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50430.2276980731677033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22960.1036674154259462No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22550.10181621157905431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13450.024.7397291
GTATCAA35600.024.042911
AATACCG659.359614E-418.464765
ATCAACG46300.017.8505443
TATCAAC47300.017.8196682
TCAACGC46900.017.662854
CAACGCA47550.017.4583555
AACGCAG48700.016.9562856
CGTTACG1402.4849578E-715.7120624
CTAGGCG800.003740894615.00871853
CGCCTAA800.00375102815.00261839
CGCGCCG800.003762314614.99584412
ACGCAGA56600.014.5542697
CGCAGAG57600.014.1280288
GTATAAC2751.8189894E-1213.849371
GTCTAGC2201.2350938E-913.6671411
AGAGTAC56600.013.423840511
TAACTCG900.00819104913.335665
GAGTACT27100.013.20667212
CAGAGTA59150.013.14935610
Produced by FastQC (version 0.11.5)