FastQCFastQC Report
Mon 6 Feb 2017
SRR2077378_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077378_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2214775
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21750.042.2409931
GTATCAA48550.038.2756961
TATCAAC69300.026.6577342
TCAACGC70100.026.2534314
ATCAACG70150.026.2347163
CAACGCA71050.025.7072775
AACGCAG73300.025.0116026
ACGCAGA86000.021.1462637
CGCAGAG88350.020.5703228
GTACATG58650.020.3347111
AGAGTAC89250.019.39778311
GAGTACT62550.019.07679612
TACATGG60850.019.0245842
CAGAGTA94600.018.51866110
GCAGAGT96650.018.3042749
AGTACTT66200.018.28545413
ACATGGG61950.018.0145783
CGAATCG1351.5839383E-417.87977437
GTACTTT68450.017.33054414
GTATAGG6350.015.8333141
Produced by FastQC (version 0.11.5)