FastQCFastQC Report
Mon 6 Feb 2017
SRR2077377_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077377_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1674371
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51510.30763791298344273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24710.14757780683014696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23450.1400525928841338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14000.025.503981
GTATCAA33350.023.3373661
GTTCTAG2850.017.5958841
TTAGTCG801.8616454E-417.5057439
TATCAAC45500.016.946862
ATCAACG45350.016.504773
TCAACGC45250.016.405134
CAACGCA46300.016.1559775
AACGCAG46550.016.0629736
GTAGCCC2800.014.2950983
GTCTTAC3100.014.235641
TAGTCGG1000.001016224514.00375640
GTGTACG1151.8038471E-413.9543031
ACGCAGA53100.013.780367
CGCAGAG54300.013.3285468
GTCGGTA900.00821889213.32854528
GAGTACT25600.013.19838312
GTACATG57900.013.06106951
CCCTATA1551.2048931E-512.9213582
CGTACAC1253.725536E-412.8084083
Produced by FastQC (version 0.11.5)